LOCUS       pCS2FA_CO_Tol2_T        6034 bp    DNA     circular UNA 04-SEP-2019
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...6y-ai3fq98
VERSION     urn.local...6y-ai3fq98
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..4063
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of 396.pCS2-transposase with
                     BamHI and XbaI - ""Backbone"" (concatenated sequence 1)"
     misc_feature    1..4
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     polyA_signal    29..226
     misc_feature    complement(274..293)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="T3 promoter (20pb)"
     promoter        complement(275..294)
                     /standard_name="T3"
     misc_feature    complement(275..294)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="T3"
     misc_feature    complement(277..293)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="T3 promoter (17bp)"
     primer_bind     complement(310..331)
                     /standard_name="M13R"
     misc_feature    complement(315..331)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="M13-R (-26); M13-R (-26)"
     misc_feature    complement(328..351)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="M13-R (-46); M13-R (-46)"
     misc_feature    complement(395..414)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pCNH200 8249 F; pCNH200_8249F"
     misc_feature    complement(598..617)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="VR"
     rep_origin      complement(658..1331)
     CDS             complement(1479..2336)
                     /standard_name="ampR"
     primer_bind     2253..2275
                     /standard_name="pStuH Flank Rev"
     rep_origin      complement(2599..2905)
     promoter        3003..3980
                     /standard_name="sCMV IE94"
     promoter        4015..4031
                     /standard_name="SP6"
     misc_feature    4060..4
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of CO Tol2 transposase  BamHI
                     XbaI with BamHI and XbaI- Insert (concatenated sequence
                     2)"
     misc_feature    4060..4063
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <4060..4075
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="GGGACAAGTTTGTACAAAAAAGCAGGCTTA"
     primer_bind     4071..4104
                     /standard_name="CO Tol2 TPase NcoI For"
     CDS             4076..6022
                     /standard_name="CO Tol2 TPase"
     misc_feature    4150
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    4156
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="C"
     primer_bind     4900..4929
                     /standard_name="CO Tol2 TPase 845 For "
     misc_feature    complement(5076..5100)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="CO Tol2 TPase 1045 Rev"
     misc_feature    5584
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     misc_feature    5593
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="A"
     misc_feature    complement(5991..2)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="CO Tol2 TPase AscI Rev"
ORIGIN      
        1 ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt
       61 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
      121 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      181 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc
      241 gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc
      301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1141 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2461 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     2941 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagtcgacca
     3001 tagccaattc aatatggcgt atatggactc atgccaattc aatatggtgg atctggacct
     3061 gtgccaattc aatatggcgt atatggactc gtgccaattc aatatggtgg atctggaccc
     3121 cagccaattc aatatggcgg acttggcacc atgccaattc aatatggcgg acttggcact
     3181 gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg gtcttggccc
     3241 tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca tattggctat
     3301 atgccaggat caatatatag gcaatatcca atatggccct atgccaatat ggctattggc
     3361 caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg ggttttccta
     3421 ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg gcttcctaat
     3481 accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc tttcctattg
     3541 acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg tcaattacgg
     3601 taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac cattgacgtc
     3661 aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga cgtcaatgac
     3721 ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg caagtacatt
     3781 actattggaa gtacgccagg gtacattggc agtactccca ttgacgtcaa tggcggtaaa
     3841 tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg gcaatgacgc
     3901 aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag gtctatataa
     3961 gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
     4021 gtgacactat agaatacaag ctacttgttc tttttgcagg atccggccgg ccaccatgga
     4081 agaggtatgt gacagtagtg cggcggcttc ttctactgtt caaaaccaac ctcaggatca
     4141 ggaacatccc tggccatatc ttcgagaatt cttttccctg tctggcgtta ataaggatag
     4201 ctttaagatg aagtgcgtgt tgtgccttcc gttgaacaag gaaatatccg cattcaagtc
     4261 ttctcctagt aatctccgaa agcacataga gaggatgcac ccaaattatc tgaagaatta
     4321 ttcaaaattg acagcacaga agaggaaaat aggaactagc actcatgcaa gcagttcaaa
     4381 acaacttaag gtcgattctg tgtttccagt aaagcatgtt agtccagtca cagtaaacaa
     4441 agcaatcctt cgatacataa ttcaaggtct gcatccattc tcaacggtgg atcttcccag
     4501 cttcaaggaa cttatttcca cgctgcagcc cggcatttca gtaatcacca ggccaacatt
     4561 gcggtccaag atcgccgagg ctgcgctgat tatgaaacag aaggttactg ccgcgatgtc
     4621 agaagtcgag tggatagcta ctactaccga ctgctggact gcaaggcgaa aaagtttcat
     4681 tggcgtgaca gcacactgga tcaatcctgg tagcctcgag aggcactccg ctgccctggc
     4741 ttgtaaacgc cttatgggga gtcatacttt tgaagtcctt gcatctgcaa tgaatgatat
     4801 ccacagtgag tatgagattc gtgacaaggt tgtttgtact acaacagatt caggtagcaa
     4861 ctttatgaaa gctttccgtg tattcggagt tgagaacaat gatatcgaaa cagaagccag
     4921 aaggtgcgag agtgacgaca cggactccga agggtgtggt gagggttccg atggcgttga
     4981 atttcaggac gcgtctaggg ttcttgacca agatgacggc tttgaatttc agttgccgaa
     5041 acatcagaaa tgtgcgtgcc atctgctcaa tcttgtaagt tcagtcgacg cccagaaagc
     5101 tctttctaac gaacactata agaaactcta tagaagcgtc ttcgggaagt gtcaagcgct
     5161 ctggaacaag tccagccgtt ctgcacttgc ggcagaggct gtagaatccg aaagtcgcct
     5221 gcaactgttg cgacccaatc aaacgcgatg gaactcaacc ttcatggccg tggaccggat
     5281 tctccagatc tgcaaggaag cgggcgaggg tgccctccgc aatatttgta cttctttgga
     5341 ggtaccaatg tttaatccgg cagaaatgct tttcttgacg gaatgggcca atacaatgcg
     5401 tccggtggca aaggttcttg acattctgca ggcggaaaca aatactcaac tcggttggct
     5461 gctgccgagc gtacaccaac tctccctgaa gctccagcgt ctccatcata gtttgcggta
     5521 ctgcgacccg ctggtagatg cgctccagca agggattcag actcgcttta aacatatgtt
     5581 cgaagatcca gagataatcg ctgcggcgat actgcttccc aaattcagaa caagctggac
     5641 taatgacgag accatcatca agcgggggat ggattacata cgggtacacc ttgaaccact
     5701 cgaccataag aaagaactgg caaactcttc ttccgatgat gaggacttct tcgcttcact
     5761 taaaccaacg acgcatgaag cgtcaaagga gctcgacggt tatctcgcgt gcgtttcaga
     5821 caccagagaa tccctcttga cctttcccgc gatatgcagc ttgtccataa agaccaatac
     5881 gccactgcct gctagcgctg cctgtgaacg tctcttttcc actgccgggt tgctctttag
     5941 cccaaagaga gccaggcttg acacgaacaa ctttgagaac caacttctct tgaagctcaa
     6001 ccttaggttt tacaacttcg agtagggcgc gcct
//
