LOCUS Exported 9414 bp ds-DNA circular SYN 11-FEB-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJC49_pDBR3_K18-mScarletI (1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9414) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Feb 11, 2020 from SnapGene Viewer 3.3.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9414 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 844..1024 /note="HIV-1 5 LTR" misc_feature 1135..1179 /note="HIV-1 psi pack" misc_feature 1695..1928 /note="RRE" misc_feature 2432..2608 /note="cppt" misc_feature 2663..3332 /note="UCOE" misc_feature 3339..5050 /note="cagg" misc_feature 5057..5062 /note="XhoI" misc_feature 5062..5067 /note="NheI" /note="note=""direct strand translation""translation=""AS""" misc_feature 5072..5473 /note="K18" misc_feature 5091..5098 /note="NotI" /note="note=""direct strand translation""translation=""AAX""" misc_feature 5487..5494 /note="NotI" /note="note=""direct strand translation"" translation=""AAX""" misc_feature 5501..6196 /note="mScarletI" misc_feature 6197..6204 /note="PmeI" /note="note=""direct strand translation""translation=""V*""" misc_feature 6205..6212 /note="AsisI" /note="note=""direct strand translation""translation=""AIX""" misc_feature 6303..6891 /note="WPRE" CDS complement(6774..6785) /note="FactorXa site" LTR 7420..7600 /note="HIV-1 5 LTR" rep_origin complement(7644..8272) /direction=LEFT /note="ColE1 origin" CDS complement(8424..9083) /note="AmpR" promoter complement(9323..9351) /note="Amp prom" ORIGIN 1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctagg gccgcgtgga 2641 taaccgtatt accgccatgc atcgggaggt ggtccctgca gttacgccaa tgataacccc 2701 cgccagaaaa atcttagtag ccttcccttt ttgttttccg tgccccaact cggcggattg 2761 actcggcccc ttccggaaac acccgaatca acttctagtc aaattattgt tcacgccgca 2821 atgacccacc cctggcccgc gtctgtggaa ctgacccctg gtgtacagga gagttcgctg 2881 ctgaaagtgg tcccaaaggg gtactagttt ttaagctccc aactccccct cccccagcgt 2941 ctggaggatt ccacaccctc gcaccgcagg ggcgaggaag tgggcggagt ccggttttgg 3001 cgccagccgc tgaggctgcc aagcagaaaa gccaccgctg aggagactcc ggtcactgtc 3061 ctcgccccgc ctcccccttc cctccccttg gggaccaccg ggcgccacgc cgcgaacggt 3121 aagtgccgcg gtcgtcggcg cctccgccct ccccctaggg ccccaattcc cagcgggcgc 3181 ggcgcgcggc ccctcccccc gccgggcgcg cgcccgctgc cccgcccttc gtggccgccc 3241 ggcgtgggcg gtgccacccc tccccccggc ggcccgcgcg cagctcccgg ctccctcccc 3301 cttcggatgt ggcttgagct gtaggcgcgg agatgcatat tgattattga ctagttatta 3361 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 3421 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 3481 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 3541 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 3601 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 3661 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg 3721 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt 3781 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc 3841 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg 3901 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg 3961 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt 4021 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 4081 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 4141 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg 4201 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg 4261 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 4321 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga 4381 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc 4441 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg 4501 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt 4561 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg 4621 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg 4681 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag 4741 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg 4801 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc 4861 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag 4921 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 4981 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca 5041 ttttggcaaa gaattcctcg agctagccac catgctgcag acagcccccg tgcccatgcc 5101 agacctgaag aatgtcaagt ccaagatcgg ctccactgag aacctgaagc accagccggg 5161 aggcgggaag gtgcagataa ttaataagaa gctggatctt agcaacgtcc agtccaagtg 5221 tggctcaaag gataatatca aacacgtcct gggaggcggc agtgtgcaaa tagtctacaa 5281 accagttgac ctgagcaagg tgacctccaa gtgtggctca ttaggcaaca tccatcataa 5341 accaggaggt ggccagatgg aagtaaaatc tgagaagctt gacttcaagg acagagtcca 5401 gtcgaagatt gggtccctgg acaatatcac ccacgtccct ggcggaggaa ataaaaagat 5461 tgaaacccac aagtctagtg gtagtagcgg ccgcagtagc gtgagcaagg gcgaggcagt 5521 gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt 5581 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 5641 ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta 5701 cggctccagg gccttcatca agcaccccgc cgacatcccc gactactata agcagtcctt 5761 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt 5821 gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac 5881 caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcgtccac 5941 cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg ccctgcgcct 6001 gaaggacggc ggacgctacc tggcggactt caagaccacc tacaaggcca agaagcccgt 6061 gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc acaacgagga 6121 ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg gcggcatgga 6181 cgagctgtac aagtaagttt aaacgcgatc gcacctagag ttgaaccgac tagtaagcca 6241 ggtcgagaca tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg 6301 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 6361 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 6421 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 6481 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 6541 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 6601 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 6661 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 6721 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 6781 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 6841 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc 6901 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 6961 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 7021 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7081 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt 7141 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg 7201 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa 7261 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg 7321 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca 7381 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag 7441 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 7501 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 7561 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag 7621 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 7681 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 7741 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 7801 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 7861 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 7921 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 7981 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 8041 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 8101 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 8161 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 8221 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 8281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 8341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 8401 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 8461 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 8521 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 8581 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 8641 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 8701 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 8761 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 8821 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 8881 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 8941 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 9001 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 9061 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 9121 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 9181 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 9241 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 9301 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 9361 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac //