LOCUS       Exported               10377 bp ds-DNA     circular SYN 09-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    dTKf_G6s
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 18, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    6508..6602
                     /label=5xUAS
     misc_feature    6636..6730
                     /label=5xUAS
                     /label=5xUAS(1)
     misc_feature    6764..6858
                     /label=5xUAS
                     /label=5xUAS(2)
     misc_feature    6892..6986
                     /label=5xUAS
                     /label=5xUAS(3)
     misc_feature    7009..7236
                     /label=hsp70 promoter
     misc_feature    7264..7330
                     /label=IVS
     misc_feature    7331..7338
                     /label=NotI
     misc_feature    7341..7346
                     /label=XhoI
     misc_feature    7347..7353
                     /label=fly kozak
     misc_feature    7354..8187
                     /label=dTK1_4
     misc_feature    8188..8193
                     /label=KR dibasic
     misc_feature    8194..8220
                     /label=dTK5
     misc_feature    8221..8226
                     /label=BamHI
     misc_feature    8227..9576
                     /label=opGCaMP6s
     primer_bind     8227..8248
                     /label=Ins_GCaMP_Fw
     misc_feature    8227..8235
                     /label=GCaMP_clone_F
     misc_feature    8227..8229
                     /label=start codon
     primer_bind     complement(8267..8287)
                     /label=Ins_GCaMP_Rv
     misc_feature    8611..8630
                     /label=G6s_GTF
     misc_feature    9123..9142
                     /label=G6s_GTR
     misc_feature    complement(9548..9576)
                     /label=Primer_Reverse_GC6s
     misc_feature    complement(9555..9576)
                     /label=GCaMP_clone_R
     misc_feature    9577..9604
                     /label=Primer_Forward_GC
     misc_feature    9577..9582
                     /label=XbaI
     misc_feature    9584..10276
                     /label=SV40
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
     6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     6721 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg
     6781 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
     6841 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact
     6901 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     6961 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata
     7021 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     7081 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     7141 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     7201 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa
     7261 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     7321 tttacttcag gcggccgcgg ctcgagaatc aaaatgcgcc cactcagcgg cctgattgct
     7381 ttggctctgc tcttgctctt gctgttgacc gcccctagct cggctgctga tacagaaacc
     7441 gagagtagtg gctcgccact gacccccggt gctgaagagc cccggcgagt tgtaaaacga
     7501 gcgcctacgt cgtcgtttat aggaatgcga ggtaaaaaag atgaggaaca cgacaccagt
     7561 gaaggcaatt ggctcggctc cggccctgac ccactggatt acgcggatga ggaggcggac
     7621 agttcgtatg cggagaatgg ccgccgcctg aagaaagcgc cactcgcttt cgttggtttg
     7681 cggggtaaga agtttattcc gattaataat cgtctgagcg atgtgctcca gagcctcgaa
     7741 gaagagcgcc tccgggactc gttgctgcag gattttttcg atcgcgtagc cggaagggat
     7801 ggatcggccg tcggtaaaag ggctccaacc ggttttactg gcatgcgtgg taaaaggccc
     7861 gcactcttgg cgggagatga cgacgctgag gcggacgagg ctacggagct gcaacaaaaa
     7921 agggctcctg tgaactcctt tgtgggtatg aggggaaaaa aagatgttag ccatcaacac
     7981 tacaaaaggg ctgctctgtc ggacttttgg cacacctttt tcaagaaaag ctacgatctg
     8041 cgaggtaagc agcagcgctt cgctgacttt aactcgaaat tcgttgcagt ccggggaaag
     8101 aaatccgatt tggaaggtaa tggagtaggt attggtgatg accacgagca agcgctcgtc
     8161 catccctggc tgtatttgtg gggcgaaaag cgtgctccaa acggcttctt gggaatgcga
     8221 ggatccatgg gatcgcatca ccaccatcat cacggaatgg cgtccatgac aggcggacaa
     8281 cagatgggac gagatttgta cgatgatgac gacaaggatc tggcgacgat ggtggatagc
     8341 tcgcgcagga agtggaacaa gacgggtcac gccgttcgtg ccataggacg gctcagttcg
     8401 cttgagaatg tgtatattaa ggcggacaaa cagaagaacg gtataaaagc caatttccac
     8461 atacgtcata acattgagga tggcggtgtg cagctcgcat atcactacca gcagaacact
     8521 cccataggtg atggcccggt gctccttccc gacaaccact atctctccgt acagtcgaag
     8581 ttgtcgaaag atcccaacga gaagcgggac cacatggtgc tgttggagtt tgttacggcc
     8641 gcgggtatca ccctgggaat ggacgagttg tataagggtg gaaccggcgg cagtatggtg
     8701 agtaagggcg aggagctctt caccggcgta gttccgatac tcgtagagtt ggatggcgac
     8761 gtgaacggcc acaagttctc cgtcagtgga gagggcgagg gcgatgccac gtacggaaag
     8821 ttgacgctga aatttatctg cacgacgggc aaattgccgg tgccatggcc gaccctcgtg
     8881 acaacgttga cttacggagt gcagtgcttc tcgcgctatc cagaccacat gaagcaacac
     8941 gactttttca agtcggccat gccggagggc tatattcaag agcgtacgat atttttcaag
     9001 gatgatggta attacaagac ccgagccgag gtcaagtttg agggagatac actggtgaac
     9061 cgaatagagc tcaagggcat cgattttaag gaggacggca atatcctggg ccataaactg
     9121 gaatacaacc tccctgacca gctcaccgag gaacagattg ccgagttcaa ggaagcattc
     9181 tcgctgttcg ataaggatgg tgacggcacc ataaccacca aggaactggg tacggtgatg
     9241 cgctccttgg gtcaaaaccc caccgaggcc gagctccagg acatgatcaa cgaagtagac
     9301 gccgacggcg acggcacgat tgacttccca gagttcctta caatgatggc acgcaaaatg
     9361 aagtaccgcg acacagagga ggagattcga gaggccttcg gagtattcga caaggacggc
     9421 aacggttaca tcagcgccgc agagctgcgg catgtgatga cgaacctggg tgaaaagctg
     9481 accgatgaag aagtcgacga aatgatcagg gaggcagata tagatggtga tggtcaggtg
     9541 aactacgagg aatttgttca gatgatgacc gctaagtcta gaggatcttt gtgaaggaac
     9601 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     9661 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     9721 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     9781 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     9841 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     9901 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     9961 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
    10021 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
    10081 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    10141 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10261 gtatcttatc atgtctggat cgatctggcc ggccgtttaa acgaattctt gaagacgaaa
    10321 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttctta
//
