LOCUS       Exported                9199 bp ds-DNA     circular SYN 11-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 11, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pBICMVp-((H2B-mC 7573 bp DNA circular SYN 23-FEB-2014 
            DEFINITION P230 pBICMVp-((H2B-mCherry)(H2B-Citrine)) ACCESSION 
            pBICMVp-((H2B-mC KEYWORDS . SOURCE Unknown. ORGANISM Unknown 
            Unclassified. REFERENCE 1 (bases 1 to 7573) AUTHORS Self JOURNAL 
            Unpublished. COMMENT SECID/File created by SciEd Central, Scientific
            & Educational Software
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1171..3410
                     /label=pCSDest
     misc_feature    complement(1..1149)
                     /label=beta-Globin intron
     primer_bind     complement(1187..1200)
                     /label=Set 1 (7)
                     /note="712"
     CDS             1196..1202
                     /codon_start=1
                     /label=attR1
                     /translation="S"
     misc_feature    complement(1209..3218)
                     /label=Untitled
     misc_feature    complement(1209..1802)
                     /label=mCherry Exon 2
                     /note="mCherry Exon 2"
     misc_feature    1212..1230
                     /label=Primer 462
                     /note="Primer 462"
     misc_feature    1531..1550
                     /label=mCherry qPCR rev
                     /note="mCherry qPCR rev"
     misc_feature    complement(1643..1664)
                     /label=mCherry qPCR fwd
                     /note="mCherry qPCR fwd"
     misc_feature    complement(1803..1824)
                     /label=3' splice site
                     /note="3' splice site"
     CDS             complement(1803..1822)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="LSFFFL"
     misc_feature    complement(1826..2094)
                     /label=Untitled
     misc_feature    complement(1851..1855)
                     /label=Branch Point?
                     /note="Branch Point?"
     misc_feature    complement(2090..2096)
                     /label=5' SS
                     /note="5' SS"
     misc_feature    complement(2095..2277)
                     /label=CFTR Exon 9
                     /note="CFTR Exon 9"
     misc_feature    2123..2144
                     /label=E9 qPCR rev
                     /note="E9 qPCR rev"
     misc_feature    complement(2145..2156)
                     /label=Splicing Silencer
                     /note="Splicing Silencer"
     misc_feature    complement(2200..2220)
                     /label=E9 qPCR fwd
                     /note="E9 qPCR fwd"
     misc_feature    complement(2209..2214)
                     /label=Splicing Enhancer
                     /note="Splicing Enhancer"
     misc_feature    complement(2242..2263)
                     /label=E9 fwd
                     /note="E9 fwd"
     misc_feature    2265..2284
                     /label=E9 fwd
                     /note="E9 fwd"
     misc_feature    complement(2277..2280)
                     /label=3' SS
                     /note="3' SS"
     misc_feature    complement(2278..2373)
                     /label=Untitled
     misc_feature    complement(2278..2317)
                     /label=Polymorphic region
                     /note="Polymorphic region"
     misc_feature    complement(2292..2317)
                     /label=TDP43 binding site
                     /note="TDP43 binding site"
     misc_feature    complement(2339..2343)
                     /label=Branch Point
                     /note="Branch Point"
     misc_feature    complement(2377..2384)
                     /label=5' splice site
                     /note="5' splice site"
     misc_feature    complement(2383..2493)
                     /label=mCherry Exon 1
                     /note="mCherry Exon 1"
     misc_feature    complement(2506..3213)
                     /label=shsfGFP2
                     /note="shsfGFP2"
     misc_feature    2709..2729
                     /label=GFP qPCR rev
                     /note="GFP qPCR rev"
     misc_feature    complement(2786..2805)
                     /label=GFP qPCR fwd
                     /note="GFP qPCR fwd"
     misc_feature    complement(3097..3114)
                     /label=Primer 571
                     /note="Primer 571"
     misc_feature    complement(3219..3390)
                     /label=Untitled
     misc_feature    complement(3220..3390)
                     /label=IBB (RchI 2-59)
                     /note="IBB (RchI 2-59)"
     misc_feature    complement(3396..3405)
                     /label=Kozak
                     /note="Kozak"
     misc_feature    3427..4094
                     /label=BICMVp
     misc_signal     complement(3427..3495)
                     /label=CMVp_min2
     misc_signal     3559..3968
                     /label=CAG enhancer
     misc_signal     3969..4094
                     /label=CMVp_min1
     primer_bind     4020..4040
                     /label=Set 1 (2)
                     /note="1179"
     misc_feature    4121..4138
                     /label=Primer 550
                     /note="Primer 550"
     misc_feature    4131..4140
                     /label=Kozak
                     /note="Kozak"
     misc_feature    4143..4838
                     /label=mTagBFP
                     /note="mTagBFP"
     CDS             4143..4838
                     /label=Untitled
     misc_feature    4659..4661
                     /label=Untitled
     CDS             4839..4844
                     /label=Untitled
     misc_feature    4845..5327
                     /label=TDP43
                     /note="TDP43"
     misc_feature    4854..5312
                     /label=RRM domains
                     /note="RRM domains"
     misc_feature    4857..4874
                     /label=RNP-2
                     /note="RNP-2"
     misc_feature    4974..4997
                     /label=RNP-1
                     /note="RNP-1"
     misc_feature    4980..4982
                     /label=Untitled
     misc_feature    4986..4988
                     /label=Untitled
     misc_feature    5118..5135
                     /label=RNP-2
                     /note="RNP-2"
     misc_feature    5121..5123
                     /label=Untitled
     misc_feature    5220..5243
                     /label=RNP-1
                     /note="RNP-1"
     misc_feature    5226..5228
                     /label=Untitled
     misc_feature    5232..5234
                     /label=Untitled
     misc_feature    5328..5783
                     /label=Low-complexity region
                     /note="Low-complexity region"
     misc_feature    5328..5783
                     /label=TDP43
                     /note="TDP43"
     misc_feature    5499..5585
                     /label=Highly conserved region
                     /note="Highly conserved region"
     primer_bind     5784..5803
                     /label=Set 1 (7)
                     /note="712"
     misc_feature    5784..5797
                     /label=Primer 746
                     /note="Primer 746"
     misc_feature    5797..5820
                     /label=Primer 551
                     /note="Primer 551"
     misc_feature    5865..7013
                     /label=beta-Globin intron
     misc_feature    7192..7865
                     /label=pUC ori
     primer_bind     7233..7250
                     /label=Set 1 (4)
                     /note="583._MP1"
     CDS             complement(8010..8870)
                     /label=AmpR
     primer_bind     9050..9068
                     /label=Set 1 (3)
                     /note="283._pQE-fwd"
     primer_bind     9053..9068
                     /label=Set 1 (6)
                     /note="587._MP"
     primer_bind     complement(9059..9075)
                     /label=Set 1 (5)
                     /note="584._MP2"
     primer_bind     complement(9082..9093)
                     /label=Set 1
                     /note="18"
ORIGIN
        1 cgagttcata agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat
       61 ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa
      121 caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca
      181 gcagggggct gtttcatata ctgatgacct ctttatagcc acctttgttc atggcagcca
      241 gcatatggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
      301 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttcccacaca ctattgcaat
      361 gaaaataaat ttcctttatt acccagaagt cagatgctca aggggcttca tgatgtcccc
      421 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac
      481 accagccacc accttctgat aggcagcctg cacctgagga gtgaattctt tgccaaaatg
      541 atgagacagc acaacaacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg
      601 aacatggtta gcagaggggc ccggtttgga ctcagagtat tttatcctca tctcaaacag
      661 tgtatatcat tgtaaccata aagagaaagg caggatgatt accaggatgt agttgtttct
      721 accaataaga atatttccac gccagccaga atttatatgc agaaatattc taccttatca
      781 tttaattata acaattgttc tctaaaactg tgctgaagta caatataatt accctgattg
      841 ccttgaaaaa aaagtgatta gagaaagtac ttacaatctg acaaataaac gaaagtgaat
      901 ttaaaaattc gttacaaatg caagctaaag tttaacgaaa aaagttacag aaaatgaaaa
      961 gaaaataaga ggagacaatg gttgtcaaca gagtagaaag tgaaagaaac aaaattatca
     1021 tgagggtcca tggtgataca agggacatct tcccattcta aacaacaccc tgaaaacttt
     1081 gccccctcca tataacatga atttttcaat agcgaaaaag aaagaacaat caagggtccc
     1141 caaactcacc ctgaagttct cagtctagag gaggatcaac cactttgtac aagaaagctg
     1201 aagctagttt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
     1261 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
     1321 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     1381 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
     1441 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
     1501 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
     1561 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     1621 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     1681 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     1741 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     1801 ccctgaggaa aaaaaaggaa agggcatgct cgccatgtgc aagatacagt gttgaatgtg
     1861 gtgcaaaata taaagatgtg gaagaaaaag ccactgaaaa taatatgagg aaataaaaaa
     1921 ctagagtaaa ttatcagtgg aacaatttct attatacaag acactacacc catacattct
     1981 cctaatgctc atgtaagaat ttctccaaaa ataccttcca gcactacaaa ctagaaaaaa
     2041 aaagagacat ggacaccaaa ttaagttctt aatagtgaag aacaaaagaa ctaccttgcc
     2101 tgctccagtg gatccagcaa ccgccaacaa ctgtcctctt tctatcttga aattaatatc
     2161 tttcaggaca ggagtaccaa gaagtgagaa attactgaag aagaggctgt catcaccatt
     2221 agaagttttt ctattgttat tgttttgttt tgctttctca aataattccc caaatccctg
     2281 ttaaaaaaaa acacacacac acacacacac acacacatca aaaataaaag atgagtttgt
     2341 cagatatttt caatagatgc ttgttttatg taccatactc acctcgccct cgccctcgat
     2401 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
     2461 gatgatggcc atgttatcct cctcgccctt gctggttccg gtgcctccac tttcatcttc
     2521 acctttagtg ataccggctg ccgtcacgaa ctccttcagg accatgtgat cgcgcttctc
     2581 gttcgggtct ttgctcagcg tggtctgggt gctcagatag tggttgtccg gcaggcgcac
     2641 cgggccgtcg ccgatcgggg tgttctgctg tttgtggtcg gcttcctgct ggctgccgtc
     2701 ctcaacgttg tggcggatct tgaagttagc cttgatgccg ttcttctgct tgtcggcttc
     2761 gatacggacg ttgtggctgt tgtggttgta ctccagcttg tgacccagga tgttgccgtc
     2821 ctccttgaag tcgttgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     2881 cacctccgcg cgggtcttgt aggtgccgtc gtccttgaac tcgatggtgc gctcctggac
     2941 gtagccttcc ggcatggcgg acttgaagaa gtcgtgacgc ttcatgtggt ccgggtagcg
     3001 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtcggccacg gcaccggcag
     3061 cttgccggtg gtgcagatga acttcagggt cagcttgccc tcggtggcat cgccctcgcc
     3121 ctcgccacgc acgctgaact tgtggccgtt tacgtcgccg tccagctcga ctttgatagg
     3181 cacagtacct gtaaatagtt cctctccttt actggtacca tcatcaggga atgagcttac
     3241 atttctcctc ttcagcatct ggtcatcctt cttagctttc ctcagctcca cattgacctc
     3301 tattctgcga cgcctcattt ctgtactgtc ttttcccttg ttcttgaatc tgtgaagacg
     3361 ggcagctggt gtattagcat tctcgttggt ggatcccatg gtggcaccgg ccggtgaatt
     3421 ctccaggcga tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca
     3481 cgccacctcg acatactcga gtagttatta atagtaatca attacggggt cattagttca
     3541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3841 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3901 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3961 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4021 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     4081 ccgtcagatc cgctagggat cctctagtca gctgacgcgt taccaccggt gccaccatgg
     4141 gaagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     4201 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     4261 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     4321 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     4381 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     4441 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     4501 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     4561 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     4621 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
     4681 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     4741 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     4801 actgcgacct ccctagcaaa ctggggcaca agcttaattc cggaatgaca tccgatttaa
     4861 tagtgttggg tctcccatgg aaaacaaccg aacaggacct gaaagagtat tttagtacct
     4921 ttggagaagt tcttatggtg caggtcaaga aagatcttaa gactggtcat tcaaaggggt
     4981 ttggctttgt tcgttttacg gaatatgaaa cacaagtgaa agtaatgtca cagcgacata
     5041 tgatagatgg acgatggtgt gactgcaaac ttcctaattc taagcaaagc caagatgagc
     5101 ctttgagaag cagaaaagtg tttgtggggc gctgtacaga ggacatgact gaggatgagc
     5161 tgcgggagtt cttctctcag tacggggatg tgatggatgt cttcatcccc aagccattca
     5221 gggcctttgc ctttgttaca tttgcagatg atcagattgc gcagtctctt tgtggagagg
     5281 acttgatcat taaaggaatc agcgttcata tatccaatgc cgaacctaag cacaatagca
     5341 atagacagtt agaaagaagt ggaagatttg gtggtaatcc aggtggcttt gggaatcagg
     5401 gtggatttgg taatagcaga gggggtggag ctggtttggg aaacaatcaa ggtagtaata
     5461 tgggtggtgg gatgaacttt ggtgcgttca gcattaatcc agccatgatg gctgccgccc
     5521 aggcagcact acagagcagt tggggtatga tgggcatgtt agccagccag cagaaccagt
     5581 caggcccatc gggtaataac caaaaccaag gcaacatgca gagggagcca aaccaggcct
     5641 tcggttctgg aaataactct tatagtggct ctaattctgg tgcagcaatt ggttggggat
     5701 cagcatccaa tgcagggtcg ggcagtggtt ttaatggagg ctttggctca agcatggatt
     5761 ctaagtcttc tggctgggga atgtagaccc agctttcttg tacaaagtgg ttgatcctct
     5821 actagtaagc ttgcatgcga tatctctaga ctgagaactt cagggtgagt ttggggaccc
     5881 ttgattgttc tttctttttc gctattgaaa aattcatgtt atatggaggg ggcaaagttt
     5941 tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat
     6001 tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat
     6061 tttctgtaac ttttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact
     6121 ttcgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag
     6181 ggtaattata ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag
     6241 gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca
     6301 actacatcct ggtaatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg
     6361 agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct
     6421 tcttcttttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct catcattttg
     6481 gcaaagaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     6541 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     6601 tcatgaagcc ccttgagcat ctgacttctg ggtaataaag gaaatttatt ttcattgcaa
     6661 tagtgtgtgg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     6721 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata tgctggctgc
     6781 catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     6841 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     6901 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     6961 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaac tcgactgcat
     7021 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7081 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7141 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7441 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7681 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7921 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7981 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8041 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8101 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8161 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8221 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8281 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8341 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8401 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8461 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8521 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8581 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8641 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8701 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8761 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8821 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8881 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8941 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9001 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9061 ccctttcgtc ttcactcgac gctggtcgag ctgatacttc ccgtccgcca ggggacatgc
     9121 cggcgatgct gaaggtcgcg cgcattcccg atgaagaggc cggttaccgc ctgttgacct
     9181 ggtgggacgg gcagggcgc
//
