LOCUS       Exported                9043 bp ds-DNA     circular SYN 11-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 11, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pBICMVp-((H2B-mC 7573 bp DNA circular SYN 23-FEB-2014 
            DEFINITION P230 pBICMVp-((H2B-mCherry)(H2B-Citrine)) ACCESSION 
            pBICMVp-((H2B-mC KEYWORDS . SOURCE Unknown. ORGANISM Unknown 
            Unclassified. REFERENCE 1 (bases 1 to 7573) AUTHORS Self JOURNAL 
            Unpublished. COMMENT SECID/File created by SciEd Central, Scientific
            & Educational Software
FEATURES             Location/Qualifiers
     source          1..9043
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1171..3410
                     /label=pCSDest
     misc_feature    complement(1..1149)
                     /label=beta-Globin intron
     primer_bind     complement(1187..1200)
                     /label=Set 1 (7)
                     /note="712"
     CDS             1196..1202
                     /codon_start=1
                     /label=attR1
                     /translation="S"
     misc_feature    complement(1209..3218)
                     /label=Untitled
     misc_feature    complement(1209..1802)
                     /label=mCherry Exon 2
                     /note="mCherry Exon 2"
     misc_feature    1212..1230
                     /label=Primer 462
                     /note="Primer 462"
     misc_feature    1531..1550
                     /label=mCherry qPCR rev
                     /note="mCherry qPCR rev"
     misc_feature    complement(1643..1664)
                     /label=mCherry qPCR fwd
                     /note="mCherry qPCR fwd"
     misc_feature    complement(1803..1824)
                     /label=3' splice site
                     /note="3' splice site"
     CDS             complement(1803..1822)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="LSFFFL"
     misc_feature    complement(1826..2094)
                     /label=Untitled
     misc_feature    complement(1851..1855)
                     /label=Branch Point?
                     /note="Branch Point?"
     misc_feature    complement(2090..2096)
                     /label=5' SS
                     /note="5' SS"
     misc_feature    complement(2095..2277)
                     /label=CFTR Exon 9
                     /note="CFTR Exon 9"
     misc_feature    2123..2144
                     /label=E9 qPCR rev
                     /note="E9 qPCR rev"
     misc_feature    complement(2145..2156)
                     /label=Splicing Silencer
                     /note="Splicing Silencer"
     misc_feature    complement(2200..2220)
                     /label=E9 qPCR fwd
                     /note="E9 qPCR fwd"
     misc_feature    complement(2209..2214)
                     /label=Splicing Enhancer
                     /note="Splicing Enhancer"
     misc_feature    complement(2242..2263)
                     /label=E9 fwd
                     /note="E9 fwd"
     misc_feature    2265..2284
                     /label=E9 fwd
                     /note="E9 fwd"
     misc_feature    complement(2277..2280)
                     /label=3' SS
                     /note="3' SS"
     misc_feature    complement(2278..2373)
                     /label=Untitled
     misc_feature    complement(2278..2317)
                     /label=Polymorphic region
                     /note="Polymorphic region"
     misc_feature    complement(2292..2317)
                     /label=TDP43 binding site
                     /note="TDP43 binding site"
     misc_feature    complement(2339..2343)
                     /label=Branch Point
                     /note="Branch Point"
     misc_feature    complement(2377..2384)
                     /label=5' splice site
                     /note="5' splice site"
     misc_feature    complement(2383..2493)
                     /label=mCherry Exon 1
                     /note="mCherry Exon 1"
     misc_feature    complement(2506..3213)
                     /label=shsfGFP2
                     /note="shsfGFP2"
     misc_feature    2709..2729
                     /label=GFP qPCR rev
                     /note="GFP qPCR rev"
     misc_feature    complement(2786..2805)
                     /label=GFP qPCR fwd
                     /note="GFP qPCR fwd"
     misc_feature    complement(3097..3114)
                     /label=Primer 571
                     /note="Primer 571"
     misc_feature    complement(3219..3390)
                     /label=Untitled
     misc_feature    complement(3220..3390)
                     /label=IBB (RchI 2-59)
                     /note="IBB (RchI 2-59)"
     misc_feature    complement(3396..3405)
                     /label=Kozak
                     /note="Kozak"
     misc_feature    3427..4094
                     /label=BICMVp
     misc_signal     complement(3427..3495)
                     /label=CMVp_min2
     misc_signal     3559..3968
                     /label=CAG enhancer
     misc_signal     3969..4094
                     /label=CMVp_min1
     primer_bind     4020..4040
                     /label=Set 1 (2)
                     /note="1179"
     misc_feature    4121..4138
                     /label=Primer 550
                     /note="Primer 550"
     misc_feature    4131..4140
                     /label=Kozak
                     /note="Kozak"
     misc_feature    4143..4838
                     /label=mTagBFP
                     /note="mTagBFP"
     CDS             4143..4838
                     /label=Untitled
     misc_feature    4659..4661
                     /label=Untitled
     CDS             4839..4844
                     /label=Untitled
     misc_feature    4845..5171
                     /label=TDP43
                     /note="TDP43"
     misc_feature    4857..5075
                     /label=Ub-like domain
                     /note="Ub-like domain"
     misc_feature    5172..5627
                     /label=Low-complexity region
                     /note="Low-complexity region"
     misc_feature    5172..5627
                     /label=TDP43
                     /note="TDP43"
     misc_feature    5343..5429
                     /label=Highly conserved region
                     /note="Highly conserved region"
     primer_bind     5628..5647
                     /label=Set 1 (7)
                     /note="712"
     misc_feature    5628..5641
                     /label=Primer 746
                     /note="Primer 746"
     misc_feature    5641..5664
                     /label=Primer 551
                     /note="Primer 551"
     misc_feature    5709..6857
                     /label=beta-Globin intron
     misc_feature    7036..7709
                     /label=pUC ori
     primer_bind     7077..7094
                     /label=Set 1 (4)
                     /note="583._MP1"
     CDS             complement(7854..8714)
                     /label=AmpR
     primer_bind     8894..8912
                     /label=Set 1 (3)
                     /note="283._pQE-fwd"
     primer_bind     8897..8912
                     /label=Set 1 (6)
                     /note="587._MP"
     primer_bind     complement(8903..8919)
                     /label=Set 1 (5)
                     /note="584._MP2"
     primer_bind     complement(8926..8937)
                     /label=Set 1
                     /note="18"
ORIGIN
        1 cgagttcata agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat
       61 ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa
      121 caaaatataa aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca
      181 gcagggggct gtttcatata ctgatgacct ctttatagcc acctttgttc atggcagcca
      241 gcatatggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
      301 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttcccacaca ctattgcaat
      361 gaaaataaat ttcctttatt acccagaagt cagatgctca aggggcttca tgatgtcccc
      421 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac
      481 accagccacc accttctgat aggcagcctg cacctgagga gtgaattctt tgccaaaatg
      541 atgagacagc acaacaacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg
      601 aacatggtta gcagaggggc ccggtttgga ctcagagtat tttatcctca tctcaaacag
      661 tgtatatcat tgtaaccata aagagaaagg caggatgatt accaggatgt agttgtttct
      721 accaataaga atatttccac gccagccaga atttatatgc agaaatattc taccttatca
      781 tttaattata acaattgttc tctaaaactg tgctgaagta caatataatt accctgattg
      841 ccttgaaaaa aaagtgatta gagaaagtac ttacaatctg acaaataaac gaaagtgaat
      901 ttaaaaattc gttacaaatg caagctaaag tttaacgaaa aaagttacag aaaatgaaaa
      961 gaaaataaga ggagacaatg gttgtcaaca gagtagaaag tgaaagaaac aaaattatca
     1021 tgagggtcca tggtgataca agggacatct tcccattcta aacaacaccc tgaaaacttt
     1081 gccccctcca tataacatga atttttcaat agcgaaaaag aaagaacaat caagggtccc
     1141 caaactcacc ctgaagttct cagtctagag gaggatcaac cactttgtac aagaaagctg
     1201 aagctagttt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
     1261 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
     1321 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     1381 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
     1441 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
     1501 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
     1561 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     1621 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     1681 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     1741 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     1801 ccctgaggaa aaaaaaggaa agggcatgct cgccatgtgc aagatacagt gttgaatgtg
     1861 gtgcaaaata taaagatgtg gaagaaaaag ccactgaaaa taatatgagg aaataaaaaa
     1921 ctagagtaaa ttatcagtgg aacaatttct attatacaag acactacacc catacattct
     1981 cctaatgctc atgtaagaat ttctccaaaa ataccttcca gcactacaaa ctagaaaaaa
     2041 aaagagacat ggacaccaaa ttaagttctt aatagtgaag aacaaaagaa ctaccttgcc
     2101 tgctccagtg gatccagcaa ccgccaacaa ctgtcctctt tctatcttga aattaatatc
     2161 tttcaggaca ggagtaccaa gaagtgagaa attactgaag aagaggctgt catcaccatt
     2221 agaagttttt ctattgttat tgttttgttt tgctttctca aataattccc caaatccctg
     2281 ttaaaaaaaa acacacacac acacacacac acacacatca aaaataaaag atgagtttgt
     2341 cagatatttt caatagatgc ttgttttatg taccatactc acctcgccct cgccctcgat
     2401 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
     2461 gatgatggcc atgttatcct cctcgccctt gctggttccg gtgcctccac tttcatcttc
     2521 acctttagtg ataccggctg ccgtcacgaa ctccttcagg accatgtgat cgcgcttctc
     2581 gttcgggtct ttgctcagcg tggtctgggt gctcagatag tggttgtccg gcaggcgcac
     2641 cgggccgtcg ccgatcgggg tgttctgctg tttgtggtcg gcttcctgct ggctgccgtc
     2701 ctcaacgttg tggcggatct tgaagttagc cttgatgccg ttcttctgct tgtcggcttc
     2761 gatacggacg ttgtggctgt tgtggttgta ctccagcttg tgacccagga tgttgccgtc
     2821 ctccttgaag tcgttgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     2881 cacctccgcg cgggtcttgt aggtgccgtc gtccttgaac tcgatggtgc gctcctggac
     2941 gtagccttcc ggcatggcgg acttgaagaa gtcgtgacgc ttcatgtggt ccgggtagcg
     3001 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtcggccacg gcaccggcag
     3061 cttgccggtg gtgcagatga acttcagggt cagcttgccc tcggtggcat cgccctcgcc
     3121 ctcgccacgc acgctgaact tgtggccgtt tacgtcgccg tccagctcga ctttgatagg
     3181 cacagtacct gtaaatagtt cctctccttt actggtacca tcatcaggga atgagcttac
     3241 atttctcctc ttcagcatct ggtcatcctt cttagctttc ctcagctcca cattgacctc
     3301 tattctgcga cgcctcattt ctgtactgtc ttttcccttg ttcttgaatc tgtgaagacg
     3361 ggcagctggt gtattagcat tctcgttggt ggatcccatg gtggcaccgg ccggtgaatt
     3421 ctccaggcga tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca
     3481 cgccacctcg acatactcga gtagttatta atagtaatca attacggggt cattagttca
     3541 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3601 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3661 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3721 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3781 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3841 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3901 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3961 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4021 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     4081 ccgtcagatc cgctagggat cctctagtca gctgacgcgt taccaccggt gccaccatgg
     4141 gaagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     4201 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     4261 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     4321 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     4381 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     4441 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     4501 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     4561 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     4621 ccctgaagct cgtgggcggg agccatctga tcgcaaacgc caagaccaca tatagatcca
     4681 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     4741 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     4801 actgcgacct ccctagcaaa ctggggcaca agcttaattc cggaatgtct gaatatattc
     4861 gggtaaccga agatgagaac gatgagccca ttgaaatacc atcggaagac gatgggacgg
     4921 tgctgctctc cacggttaca gcccagtttc caggggcgtg tgggcttcgc tacaggaatc
     4981 cagtgtctca gtgtatgaga ggtgtccggc tggtagaagg aattctgcat gccccagatg
     5041 ctggctgggg aaatctggtg tatgttgtca actatccaaa agataacaaa agaaaaatgg
     5101 atgagacaga tgcttcatca gcagtgaaag tgaaaagagc agtccagaaa acatcctcca
     5161 atgccgaacc taagcacaat agcaatagac agttagaaag aagtggaaga tttggtggta
     5221 atccaggtgg ctttgggaat cagggtggat ttggtaatag cagagggggt ggagctggtt
     5281 tgggaaacaa tcaaggtagt aatatgggtg gtgggatgaa ctttggtgcg ttcagcatta
     5341 atccagccat gatggctgcc gcccaggcag cactacagag cagttggggt atgatgggca
     5401 tgttagccag ccagcagaac cagtcaggcc catcgggtaa taaccaaaac caaggcaaca
     5461 tgcagaggga gccaaaccag gccttcggtt ctggaaataa ctcttatagt ggctctaatt
     5521 ctggtgcagc aattggttgg ggatcagcat ccaatgcagg gtcgggcagt ggttttaatg
     5581 gaggctttgg ctcaagcatg gattctaagt cttctggctg gggaatgtag acccagcttt
     5641 cttgtacaaa gtggttgatc ctctactagt aagcttgcat gcgatatctc tagactgaga
     5701 acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt gaaaaattca
     5761 tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt
     5821 atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat
     5881 tgtctcctct tattttcttt tcattttctg taactttttt cgttaaactt tagcttgcat
     5941 ttgtaacgaa tttttaaatt cactttcgtt tatttgtcag attgtaagta ctttctctaa
     6001 tcactttttt ttcaaggcaa tcagggtaat tatattgtac ttcagcacag ttttagagaa
     6061 caattgttat aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg
     6121 gaaatattct tattggtaga aacaactaca tcctggtaat catcctgcct ttctctttat
     6181 ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc
     6241 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     6301 ttgttgtgct gtctcatcat tttggcaaag aattcactcc tcaggtgcag gctgcctatc
     6361 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     6421 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctgggtaat
     6481 aaaggaaatt tattttcatt gcaatagtgt gtgggaattt tttgtgtctc tcactcggaa
     6541 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     6601 caacatatgc catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat
     6661 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     6721 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     6781 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     6841 cttctcttat gaactcgact gcattaatga atcggccaac gcgcggggag aggcggtttg
     6901 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6961 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7021 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7081 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7141 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7201 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7261 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     7321 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7381 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7441 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7501 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7561 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7621 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7681 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7741 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7801 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7861 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7921 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7981 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8041 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8101 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8161 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8221 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8281 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8341 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8401 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8461 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8521 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8581 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8641 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8701 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8761 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8821 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     8881 tataaaaata ggcgtatcac gaggcccttt cgtcttcact cgacgctggt cgagctgata
     8941 cttcccgtcc gccaggggac atgccggcga tgctgaaggt cgcgcgcatt cccgatgaag
     9001 aggccggtta ccgcctgttg acctggtggg acgggcaggg cgc
//
