LOCUS       pBX_The3_BbsISit        6010 bp ds-DNA     circular     01-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBX The3 BbsI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 19, 2016 from SnapGene 3.1.0
            http://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2649..2800
                     /label=seq_C9_OK
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             458..1252
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     P
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     S
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     Q
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     A LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /locus_tag="aminoglycoside phosphotransferase from Tn5"
                     /label=aminoglycoside phosphotransferase from Tn5
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2270..2316
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /locus_tag="rrnB T1 terminator"
                     /label=rrnB T1 terminator
                     /ApEinfo_fwdcolor=#9d1b1c
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      1600..1646
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /locus_tag="rrnB T1 terminator"
                     /label=rrnB T1 terminator(1)
                     /ApEinfo_label=rrnB T1 terminator
                     /ApEinfo_fwdcolor=#9d1b1c
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2740..2783
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /locus_tag="Escherichia coli rrnB"
                     /label=Escherichia coli rrnB
                     /ApEinfo_fwdcolor=#9d1b1c
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1979..2064
                     /note="The3 sgRNA"
                     /locus_tag="The3 sgRNA"
                     /label=The3 sgRNA
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2613..2628
                     /label=reverse consensus primer_XylA_R
                     /ApEinfo_fwdcolor=#e6e6e6
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2070..2113
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /locus_tag="Escherichia coli rrnB"
                     /label=Escherichia coli rrnB(1)
                     /ApEinfo_label=Escherichia coli rrnB
                     /ApEinfo_fwdcolor=#9d1b1c
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2373..2392
                     /label=JDP213
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2256..2269)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="SK primer"
                     /label=SK primer
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(2804..2822)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2599..2628
                     /label=CRISPR_plasmid_R
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2832..2848)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label=M13 fwd
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3556..4218)
                     /codon_start=1
                     /product="replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     H
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYV
                     V
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGE
                     E
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
                     /locus_tag="replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=replication protein for the broad-host-range
                     plasmid pBBR1 from Bordetella bronchiseptica
                     /ApEinfo_fwdcolor=#e9d024
                     /ApEinfo_revcolor=#e9d024
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2765..2794
                     /label=dual_insert_short_dwn_R
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4219..4988
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
                     /locus_tag="pBBR1 oriV"
                     /label=pBBR1 oriV
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2629..2647)
                     /label=blaA
                     /ApEinfo_fwdcolor=#66ccff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1661..1958
                     /label=pxyl
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2629..2648)
                     /label=full_guide1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2629..2648
                     /label=ultramer_blaA2
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2239..2269
                     /label=pBXMCS-2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1979..2008
                     /label=ultramer_cpaA
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2795..3257
                     /label=pBXMCS-2(1)
                     /ApEinfo_label=pBXMCS-2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1459..1480
                     /label=MGOP224
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2848..2867
                     /label=MGP053
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2649..2734
                     /note="The3 sgRNA"
                     /locus_tag="The3 sgRNA"
                     /label=The3 sgRNA(1)
                     /ApEinfo_label=The3 sgRNA
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2331..2628
                     /label=pxyl(1)
                     /ApEinfo_label=pxyl
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2599..2628
                     /label=ultramer_cpaA(1)
                     /ApEinfo_label=ultramer_cpaA
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2649..2678
                     /label=ultramer_cpaA(2)
                     /ApEinfo_label=ultramer_cpaA
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1959..1978
                     /label=ultramer_cpaA(3)
                     /ApEinfo_label=ultramer_cpaA
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#cccccc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1959..1978
                     /label=guide1cpaA
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatata agctttcagt
     1561 cggcgcgaat gctccacgcc gcgcccagcg gcgcggatcc aaataaaacg aaaggctcag
     1621 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgc tgcagccagc cgtggtcggg
     1681 cagcaggtag aaggcgccct cgtcctgatc ctcgcccgaa acctccagcc cccggtcgat
     1741 ggcttcgacg acatagccgg ccgcgcggca ggtgtcggtg agcgcggcca gcagggcggc
     1801 ttcctggtca ggggtcaggt cggtcatggg caagaggtcc aggtcgtggt ttgtcggcgg
     1861 cttctagcat ggaccgcccg cgcccgtgag gccgaggatt tcgcgctggt cagacaacct
     1921 acttgccgtc cccacatgtt agcgctacca agtgccgaGA GCGTGACGCG AGTGGCTTgt
     1981 tttagagctg tgaaaacagc gagttaaaat aaggcttagt ccgtactcaa cttgaaaagg
     2041 tggcaccgat tcggtgtttt ttttttttga taaaacgaaa ggcccagtct ttcgactgag
     2101 cctttcgttt tataaacggg tcttgagggg ttttttaagc ttctgcagct ggatccatgg
     2161 atatctagat ttccgcaaaa taaaaaaggg gacctctagg gtccccaatt aattagctaa
     2221 agccgctagc ccatgggaat tcctgcagcc cgggggatcc actagttctc aaataaaacg
     2281 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgc tgcagccagc
     2341 cgtggtcggg cagcaggtag aaggcgccct cgtcctgatc ctcgcccgaa acctccagcc
     2401 cccggtcgat ggcttcgacg acatagccgg ccgcgcggca ggtgtcggtg agcgcggcca
     2461 gcagggcggc ttcctggtca ggggtcaggt cggtcatggg caagaggtcc aggtcgtggt
     2521 ttgtcggcgg cttctagcat ggaccgcccg cgcccgtgag gccgaggatt tcgcgctggt
     2581 cagacaacct acttgccgtc cccacatgtt agcgctacca agtgccgaCC AGGATCAGGC
     2641 GCTTCATGgt tttagagctg tgaaaacagc gagttaaaat aaggcttagt ccgtactcaa
     2701 cttgaaaagg tggcaccgat tcggtgtttt ttttttttga taaaacgaaa ggcccagtct
     2761 ttcgactgag cctttcgttt tataaacggg tcttccaatt cgccctatag tgagtcgtat
     2821 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2881 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2941 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
     3001 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3061 aggccgtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     3121 ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
     3181 cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag ccgcttatgt
     3241 ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt gcttctcaaa
     3301 tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg atatgatcat
     3361 ttattctgcc tcccagagcc tgataaaaac ggtgaatccg ttagcgaggt gccgccggct
     3421 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     3481 aggtataggg cggcgaggcg gctacagccg atagtctgga acagcgcact tacgggttgc
     3541 tgcgcaaccc aagtgctacc ggcgcggcag cgtgacccgt gtcggcggct ccaacggctc
     3601 gccatcgtcc agaaaacacg gctcatcggg catcggcagg cgctgctgcc cgcgccgttc
     3661 ccattcctcc gtttcggtca aggctggcag gtctggttcc atgcccggaa tgccgggctg
     3721 gctgggcggc tcctcgccgg ggccggtcgg tagttgctgc tcgcccggat acagggtcgg
     3781 gatgcggcgc aggtcgccat gccccaacag cgattcgtcc tggtcgtcgt gatcaaccac
     3841 cacggcggca ctgaacaccg acaggcgcaa ctggtcgcgg ggctggcccc acgccacgcg
     3901 gtcattgacc acgtaggccg acacggtgcc ggggccgttg agcttcacga cggagatcca
     3961 gcgctcggcc accaagtcct tgactgcgta ttggaccgtc cgcaaagaac gtccgatgag
     4021 cttggaaagt gttttctggc tgaccaccac ggcgttctgg tggcccatct gcgccacgag
     4081 gtgatgcagc agcattgccg ccgtgggttt cctcgcaata agcccggccc acgcctcatg
     4141 cgctttgcgt tccgtttgca cccagtgacc gggcttgttc ttggcttgaa tgccgatttc
     4201 tctggactgc gtggccatgc ttatctccat gcggtagggt gccgcacggt tgcggcacca
     4261 tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg
     4321 cagtcaatta cagattttct ttaacctacg caatgagcta ttgcgggggg tgccgcaatg
     4381 agctgttgcg tacccccctt ttttaagttg ttgattttta agtctttcgc atttcgccct
     4441 atatctagtt ctttggtgcc caaagaaggg cacccctgcg gggttccccc acgccttcgg
     4501 cgcggctccc cctccggcaa aaagtggccc ctccggggct tgttgatcga ctgcgcggcc
     4561 ttcggccttg cccaaggtgg cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc
     4621 tcggggggca ggcgggcggg cttcgccttc gactgccccc actcgcatag gcttgggtcg
     4681 ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc gcgagccttg acccgccttc
     4741 cacttggtgt ccaaccggca agcgaagcgc gcaggccgca ggccggaggc ttttccccag
     4801 agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc gcagttggag ccggtgggta
     4861 tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc
     4921 agcctgtcca tcagcttgtc cagcagggtt gtccacgggc cgagcgaagc gagccagccg
     4981 gtggccgctc gcggccatcg tccacatatc cacgggctgg caagggagcg cagcgaccgc
     5041 gcagggcgaa gcccggagag caagcccgta gggcgccgca gccgccgtag gcggtcacga
     5101 ctttgcgaag caaagtctag tgagtatact caagcattga gtggcccgcc ggaggcaccg
     5161 ccttgcgctg cccccgtcga gccggttgga caccaaaagg gaggggcagg catggcggca
     5221 tacgcgatca tgcgatgcaa gaagctggcg aaaatgggca acgtggcggc cagtctcaag
     5281 cacgcctacc gcgagcgcga gacgcccaac gctgacgcca gcaggacgcc agagaacgag
     5341 cactgggcgg ccagcagcac cgatgaagcg atgggccgac tgcgcgagtt gctgccagag
     5401 aagcggcgca aggacgctgt gttggcggtc gagtacgtca tgacggccag cccggaatgg
     5461 tggaagtcgg ccagccaaga acagcaggcg gcgttcttcg agaaggcgca caagtggctg
     5521 gcggacaagt acggggcgga tcgcatcgtg acggccagca tccaccgtga cgaaaccagc
     5581 ccgcacatga ccgcgttcgt ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag
     5641 ttcatcggca acaaagcgca gatgacccgc gaccagacca cgtttgcggc cgctgtggcc
     5701 gatctagggc tgcaacgggg catcgagggc agcaaggcac gtcacacgcg cattcaggcg
     5761 ttctacgagg ccctggagcg gccaccagtg ggccacgtca ccatcagccc gcaagcggtc
     5821 gagccacgcg cctatgcacc gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt
     5881 gagacgccgg aagccgtggc cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct
     5941 gccctacagg ccgccgcagg agcgcgtgag atgcgcaaga aggccgatca agcccaagag
     6001 acggcccgag     
//
