LOCUS       pBS_PtH                 4729 bp ds-DNA     circular     10-SEP-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(3843..4510)
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#bee0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    930..1952
                     /locus_tag="Hygro"
                     /label="Hygro"
                     /ApEinfo_label="Hygro"
                     /ApEinfo_fwdcolor="#77bdff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 870..922
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#ffb005"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2835..3692
                     /locus_tag="Amp"
                     /label="Amp"
                     /ApEinfo_label="Amp"
                     /ApEinfo_fwdcolor="#eab0fa"
                     /ApEinfo_revcolor="#70ff6a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(2380..2686)
                     /locus_tag="F1ori"
                     /label="F1ori"
                     /ApEinfo_label="F1ori"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#bee0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    918..922
                     /locus_tag="cleavage loc"
                     /label="cleavage loc"
                     /ApEinfo_label="cleavage loc"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    258..854
                     /locus_tag="PAC"
                     /label="PAC"
                     /ApEinfo_label="PAC"
                     /ApEinfo_fwdcolor="#355ffe"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
       61 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
      121 CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGG
      181 TACCGGGCCC CCCCTCGAGG TCGACGGTAT CGATAAGCTT GCATGCCTGC AGGTCGGCCG
      241 CCACGACCGG TGCCACCATG ACTGAATACA AACCAACTGT TCGCCTGGCA ACTCGTGATG
      301 ATGTTCCACG TGCAGTTCGC ACCCTGGCTG CTGCATTTGC TGACTACCCT GCAACCCGTC
      361 ACACTGTGGA CCCAGACCGC CACATTGAAC GTGTGACTGA ACTGCAGGAG CTGTTCCTGA
      421 CCCGTGTGGG CCTGGACATT GGCAAAGTGT GGGTGGCAGA TGATGGTGCT GCTGTGGCAG
      481 TGTGGACCAC CCCTGAATCT GTTGAAGCTG GTGCAGTGTT TGCTGAGATT GGCCCACGCA
      541 TGGCAGAACT GTCTGGCAGC CGCCTGGCAG CACAACAGCA GATGGAAGGT CTGCTGGCAC
      601 CACACCGCCC AAAAGAACCT GCTTGGTTCC TGGCAACTGT GGGTGTGAGC CCTGACCACC
      661 AGGGTAAGGG CCTGGGCTCT GCAGTGGTGC TGCCTGGTGT GGAAGCAGCT GAACGTGCAG
      721 GTGTGCCTGC TTTCCTGGAG ACCTCAGCTC CACGCAACCT GCCTTTCTAT GAACGCCTGG
      781 GCTTCACTGT GACTGCTGAT GTGGAAGTGC CAGAAGGCCC ACGCACTTGG TGCATGACTC
      841 GCAAACCAGG TGCTAGCCTT GGCTCCGGCG AGGGCAGGGG AAGTCTTCTA ACATGCGGGG
      901 ACGTGGAGGA AAATCCCGGC CCACTCGGCA AAAAGCCTGA ACTCACCGCG ACGTCTGTCG
      961 AGAAGTTTCT GATCGAAAAG TTCGACAGCG TCTCCGACCT GATGCAGCTC TCGGAGGGCG
     1021 AAGAATCTCG TGCTTTCAGC TTCGATGTAG GAGGGCGTGG ATATGTCCTG CGGGTAAATA
     1081 GCTGCGCCGA TGGTTTCTAC AAAGATCGTT ATGTTTATCG GCACTTTGCA TCGGCCGCGC
     1141 TCCCGATTCC GGAAGTGCTT GACATTGGGG AATTCAGCGA GAGCCTGACC TATTGCATCT
     1201 CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG ACCTGCCTGA AACCGAACTG CCCGCTGTTC
     1261 TGCAGCCGGT CGCGGAGGCC ATGGATGCGA TCGCTGCGGC CGATCTTAGC CAGACGAGCG
     1321 GGTTCGGCCC ATTCGGACCG CAAGGAATCG GTCAATACAC TACATGGCGT GATTTCATAT
     1381 GCGCGATTGC TGATCCCCAT GTGTATCACT GGCAAACTGT GATGGACGAC ACCGTCAGTG
     1441 CGTCCGTCGC GCAGGCTCTC GATGAGCTGA TGCTTTGGGC CGAGGACTGC CCCGAAGTCC
     1501 GGCACCTCGT GCACGCGGAT TTCGGCTCCA ACAATGTCCT GACGGACAAT GGCCGCATAA
     1561 CAGCGGTCAT TGACTGGAGC GAGGCGATGT TCGGGGATTC CCAATACGAG GTCGCCAACA
     1621 TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA TGGAGCAGCA GACGCGCTAC TTCGAGCGGA
     1681 GGCATCCGGA GCTTGCAGGA TCGCCGCGGC TCCGGGCGTA TATGCTCCGC ATTGGTCTTG
     1741 ACCAACTCTA TCAGAGCTTG GTTGACGGCA ATTTCGATGA TGCAGCTTGG GCGCAGGGTC
     1801 GATGCGACGC AATCGTCCGA TCCGGAGCCG GGACTGTCGG GCGTACACAA ATCGCCCGCA
     1861 GAAGCGCGGC CGTCTGGACC GATGGCTGTG TAGAAGTACT CGCCGATAGT GGAAACCGAC
     1921 GCCCCAGCAC TCGTCCGAGG GCAAAGGAAT AGAGCGGCCG CGACTCCAGT TCGTGGCCGT
     1981 CGATCGTTTA AAGGGAGGTA GTGAGTCGAG ATATCGATAC TAGTTCTAGA GCGGCCGCCA
     2041 CCGCGGTGGA GCTCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC
     2101 GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA
     2161 CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
     2221 CAGTTGCGCA GCCTGAATGG CGAATGGAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG
     2281 CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC
     2341 CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA
     2401 GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG
     2461 ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG
     2521 CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA
     2581 ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG
     2641 TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG
     2701 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA
     2761 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT
     2821 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG
     2881 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG
     2941 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG
     3001 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG
     3061 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT
     3121 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA
     3181 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC
     3241 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC
     3301 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC
     3361 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC
     3421 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG
     3481 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG
     3541 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA
     3601 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG
     3661 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA
     3721 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT
     3781 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC
     3841 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT
     3901 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA
     3961 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG
     4021 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC
     4081 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG
     4141 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA
     4201 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT
     4261 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG
     4321 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC
     4381 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC
     4441 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC
     4501 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG
     4561 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA
     4621 GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC
     4681 ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGA 
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