LOCUS       pTR-UF-12.1_new         6023 bp    DNA     circular      4-JUN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|699884785|
COMMENT     VNTDBDATE|699884996|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTR-UF-12.1_new|
COMMENT     VNTAUTHORNAME|Mingjie Li|
COMMENT     VNTAUTHORTEL|314-747-1123|
COMMENT     VNTAUTHOREML|lim@neuro.wustl.edu|
COMMENT     VNTAUTHORAD1|Department of Neurology|
COMMENT     VNTAUTHORAD2|Washington University School of Medicine|
COMMENT     VNTAUTHORAD3|Campus Box 8111, 660 S. Euclid Ave.|
COMMENT     VNTAUTHORAD4|Saint Louis, MO 63110|
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FEATURES             Location/Qualifiers
     repeat_region   24..156
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     polyA_site      3626..3862
                     /dnas_title="bGHpolyA"
                     /vntifkey="26"
                     /label=bGHpolyA
                     /note="bGHpolyA"
     repeat_region   3897..4018
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     rep_origin      complement(4114..4573)
                     /dnas_title="f1+"
                     /vntifkey="33"
                     /label=f1+
                     /note="f1+"
     exon            complement(4977..5977)
                     /dnas_title="ApR"
                     /vntifkey="61"
                     /label=ApR
                     /note="Ampicillin Resistance"
     rep_origin      complement(6227..6452)
                     /dnas_title="ColE1-ori"
                     /vntifkey="33"
                     /label=ColE1-ori
                     /note="ColE1-ori"
     misc_feature    complement(3628..3651)
                     /dnas_title="AAV-R1 primer"
                     /vntifkey="21"
                     /label=AAV-R1 primer
     source          1..6893
                     /dnas_title="pTR-UF-12.1_new"
     misc_feature    174..537
                     /dnas_title="CMV Enhancer"
                     /vntifkey="21"
                     /label=CMV Enhancer
     misc_feature    173..1115
                     /note="CMV Enhancer & Chick b-actin promoter & SD &
                     intron in 5' UTR"
     promoter        538..820
                     /dnas_title="Chick B-actin promoter"
                     /vntifkey="29"
                     /label=Chick B-actin promoter
                     /note="Chicken B-actin Promoter"
     misc_feature    913..1019
                     /dnas_title="intron in 5'UTR"
                     /vntifkey="21"
                     /label=intron in 5'UTR
     misc_feature    912..913
                     /dnas_title="SD"
                     /vntifkey="21"
                     /label=SD
                     /note="Splicing donor"
     misc_feature    1065..1066
                     /dnas_title="SA"
                     /vntifkey="21"
                     /label=SA
                     /note="Splicing acceptor"
     misc_feature    1023..1047
                     /dnas_title="AAV-F1 primer"
                     /vntifkey="21"
                     /label=AAV-F1 primer
     misc_feature    5017..5877
                     /note="AmpR"
     misc_feature    3886..4029
                     /note="ITR"
     misc_feature    6894..7037
                     /note="ITR"
     misc_feature    1142..1147
                     /note="MCS"
     PCR_primer      1148..1153
                     /dnas_title="GCCACCATGGGTTCTCATCA"
                     /pair=""
                     /primer="GCCACCATGGGTTCTCATCA"
                     /current=0
     misc_feature    1154..2401
                     /note="G6f"
     PCR_primer      2408..2473
                     /dnas_title="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGAC
                     GTGGAGGAGAACCCTGGACCT"
                     /pair=""
                     /primer="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGG
                     AGGAGAACCCTGGACCT"
                     /current=0
     misc_feature    2408..2473
                     /note="P2A"
     PCR_primer      complement(2450..2473)
                     /dnas_title="AGGTCCAGGGTTCTCCTCCACGTC"
                     /pair=""
                     /primer="AGGTCCAGGGTTCTCCTCCACGTC"
                     /current=0
     PCR_primer      2408..2431
                     /dnas_title="GGAAGCGGAGCTACTAACTTCAGC"
                     /pair=""
                     /primer="GGAAGCGGAGCTACTAACTTCAGC"
                     /current=0
     misc_feature    complement(2480..2485)
                     /note="kozak"
     PCR_primer      2475..2485
                     /dnas_title="GCCACCATGCCAGAGCCAGCGAA"
                     /pair=""
                     /primer="GCCACCATGCCAGAGCCAGCGAA"
                     /current=1
     misc_feature    2486..3427
                     /note="vmn1r78"
     polyA_site      3427..3625
                     /dnas_title="SV40 polyA"
                     /vntifkey="26"
                     /label=SV40 polyA
                     /note="SV40 polyA"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagatc tgaattcggt accctagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      601 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
      661 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      721 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      781 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga cgctgccttc
      841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagc
     1021 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1081 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgaagatc cgaaggggtt
     1141 caagcttGCC ACCATGGTCG ACTCATCACG TCGTAAGTGG AATAAGACAG GTCACGCAGT
     1201 CAGAGCTATA GGTCGGCTGA GCTCACTCGA GAACGTCTAT ATCAAGGCCG ACAAGCAGAA
     1261 GAACGGCATC AAGGCGAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT
     1321 CGCCTACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
     1381 CCACTACCTG AGCGTGCAGT CCAAACTTTC GAAAGACCCC AACGAGAAGC GCGATCACAT
     1441 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
     1501 GGGCGGTACC GGAGGGAGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
     1561 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG
     1621 TGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT
     1681 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG
     1741 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACAT
     1801 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
     1861 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA
     1921 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACCTGCCG GACCAACTGA CTGAAGAGCA
     1981 GATCGCAGAA TTTAAAGAGG AATTCTCCCT ATTTGACAAG GACGGGGATG GGACAATAAC
     2041 AACCAAGGAG CTGGGGACGG TGATGCGGTC TCTGGGGCAG AACCCCACAG AAGCAGAGCT
     2101 GCAGGACATG ATCAATGAAG TAGATGCCGA CGGTGACGGC ACAATCGACT TCCCTGAGTT
     2161 CCTGACAATG ATGGCAAGAA AAATGAAATA CAGGGACACG GAAGAAGAAA TTAGAGAAGC
     2221 GTTCGGTGTG TTTGATAAGG ATGGCAATGG CTACATCAGT GCAGCAGAGC TTCGCCACGT
     2281 GATGACAAAC CTTGGAGAGA AGTTAACAGA TGAAGAGGTT GATGAAATGA TCAGGGAAGC
     2341 AGACATCGAT GGGGATGGTC AGGTAAACTA CGAAGAGTTT GTACAAATGA TGACAGCGAA
     2401 Ggaattcgga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     2461 gaaccctgga cctgatatcg ccaccATGAA AATGACTCCT GCAAGCTTGG CAACGGGAAT
     2521 ATTCTTTTTC TTTCATATTA CAGTGGGAAT CCTTGGTAAT TCCTCAATAC TATTTCATTA
     2581 TGTCTTTATG ATAACCACCA GAAAGCATTT AATGCCCAAA GATGTGATTA TAAAGCACTT
     2641 GACTTTTGCT AACTGCTTAA CTATCATTTC AAAAGGCATT CCAGAGACAC TGTCAGATTT
     2701 GGGATTTAAA TATTTCCTGG ATGATATTGG TTGTAAATTG GTAGTTTATA TTTACCGAAT
     2761 AATGAGGGGG ATGTCCCTGT ATGCTATGTG CCTACTGAGT TGCTTCCAAG CAATCACAAT
     2821 CAGCCCCAGC AACACCAGGT GGATAATGTT TAAACACAGA GCCACCAAGT ACATTGGTCC
     2881 CTCCTGCTCA GTCAGTTGGC TTGTGTACAT GCTTTTAAAC ATCTTGACTC CAGCAAGAGT
     2941 GTTAGGACCC AGTTATAACA AAAATGTAAC TAACATGGTG AATTATGGAT ACTGCTCATG
     3001 GTTTACTTCC AGAAATGTGG CAACTGCTCT GTATATGTTT CTACTCTGCT TCTCTGATGG
     3061 CTTGTGCCTG GGTCTCATGG CCTGCTCAAG TATTTCCATG GTGAGTGTCC TCTACAGACA
     3121 CAAGAGACAA GTCAAGCATA TCCATAGTGC TCAGCATTTT CTAAAAGTCT CTCCTGAGGA
     3181 CCAAGCCACC AAAACTATCC TCATCCTGGT TTGTACATTT GTCATCTCTT ACTCAATCTC
     3241 TTCTATACAT GTAATATTTA CAGTCTACTT CAAAGGCTCA GGACTATGGG GAGTAAGTAT
     3301 GTTTATATTT TTAGAATTAT GTTTTCCAAT ATATTTCCCA TTTGTTCTCA TCACCAGTTT
     3361 CAAGTCTAGT TGCAGCTTCT TTTTATCCTT CTGCAAAAAG AGATGGCTTC CTTCAAATAT
     3421 TGCATGatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3481 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3541 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3601 gggaggtgtg ggaggttttt tagtcgacta gagctcgctg atcagcctcg actgtgcctt
     3661 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     3721 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3781 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3841 atagcaggca tgctggggag agatctcacg tgcggaccga gcggccgcag gaacccctag
     3901 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     3961 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     4021 gcctgcaggg gcgcctgatg ctgcagccag ctggcgtaat agcgaagagg cccgcaccga
     4081 tcgcccttcc caacagttgc gtagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg
     4141 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4201 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4261 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4321 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4381 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4441 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4501 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4561 aatattaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4621 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4681 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4741 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4801 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4861 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5221 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5521 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6181 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6421 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6661 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6721 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6781 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     6841 ctccccgcgc gttggccgat tcattaatgc agctgggctg caggctcaca tgt
//

