LOCUS pTR-UF-12.1_new 6023 bp DNA circular 4-JUN-2014 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|699884785| COMMENT VNTDBDATE|699884996| COMMENT LSOWNER| COMMENT VNTNAME|pTR-UF-12.1_new| COMMENT VNTAUTHORNAME|Mingjie Li| COMMENT VNTAUTHORTEL|314-747-1123| COMMENT VNTAUTHOREML|lim@neuro.wustl.edu| COMMENT VNTAUTHORAD1|Department of Neurology| COMMENT VNTAUTHORAD2|Washington University School of Medicine| COMMENT VNTAUTHORAD3|Campus Box 8111, 660 S. Euclid Ave.| COMMENT VNTAUTHORAD4|Saint Louis, MO 63110| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT #290=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -251 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 38894328 COMMENT (CStringList) 38749468 38749576 (CObList))) FEATURES Location/Qualifiers repeat_region 24..156 /dnas_title="ITR" /vntifkey="34" /label=ITR /note="ITR" polyA_site 3611..3847 /dnas_title="bGHpolyA" /vntifkey="26" /label=bGHpolyA /note="bGHpolyA" repeat_region 3882..4003 /dnas_title="ITR" /vntifkey="34" /label=ITR /note="ITR" rep_origin complement(4099..4558) /dnas_title="f1+" /vntifkey="33" /label=f1+ /note="f1+" exon complement(4962..5962) /dnas_title="ApR" /vntifkey="61" /label=ApR /note="Ampicillin Resistance" rep_origin complement(6212..6437) /dnas_title="ColE1-ori" /vntifkey="33" /label=ColE1-ori /note="ColE1-ori" misc_feature complement(3613..3636) /dnas_title="AAV-R1 primer" /vntifkey="21" /label=AAV-R1 primer source 1..6878 /dnas_title="pTR-UF-12.1_new" misc_feature 174..537 /dnas_title="CMV Enhancer" /vntifkey="21" /label=CMV Enhancer misc_feature 173..1115 /note="CMV Enhancer & Chick b-actin promoter & SD & intron in 5' UTR" promoter 538..820 /dnas_title="Chick B-actin promoter" /vntifkey="29" /label=Chick B-actin promoter /note="Chicken B-actin Promoter" misc_feature 913..1019 /dnas_title="intron in 5'UTR" /vntifkey="21" /label=intron in 5'UTR misc_feature 912..913 /dnas_title="SD" /vntifkey="21" /label=SD /note="Splicing donor" misc_feature 1065..1066 /dnas_title="SA" /vntifkey="21" /label=SA /note="Splicing acceptor" misc_feature 1023..1047 /dnas_title="AAV-F1 primer" /vntifkey="21" /label=AAV-F1 primer misc_feature 5002..5862 /note="AmpR" misc_feature 3871..4014 /note="ITR" misc_feature 6879..7022 /note="ITR" misc_feature 1142..1147 /dnas_title="MCS" /vntifkey="21" /label=MCS misc_feature 1142..1147 /dnas_title="MCS" /vntifkey="21" /label=MCS misc_feature 1142..1147 /note="MCS" misc_feature 1154..2401 /note="G6f" PCR_primer 2408..2473 /dnas_title="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGAC GTGGAGGAGAACCCTGGACCT" /pair="" /primer="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGG AGGAGAACCCTGGACCT" /current=0 misc_feature 2408..2473 /note="P2A" PCR_primer complement(2450..2473) /dnas_title="AGGTCCAGGGTTCTCCTCCACGTC" /pair="" /primer="AGGTCCAGGGTTCTCCTCCACGTC" /current=0 PCR_primer 2408..2431 /dnas_title="GGAAGCGGAGCTACTAACTTCAGC" /pair="" /primer="GGAAGCGGAGCTACTAACTTCAGC" /current=0 misc_feature complement(2480..2485) /note="kozak" PCR_primer 2475..2485 /dnas_title="GCCACCATGCCAGAGCCAGCGAA" /pair="" /primer="GCCACCATGCCAGAGCCAGCGAA" /current=1 misc_feature 2486..3412 /note="vmn1r85" polyA_site 3412..3610 /dnas_title="SV40 polyA" /vntifkey="26" /label=SV40 polyA /note="SV40 polyA" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtagatc tgaattcggt accctagtta 181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 541 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat 601 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg 661 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc 721 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg 781 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga cgctgccttc 841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt 901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg 961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagc 1021 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt 1081 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgaagatc cgaaggggtt 1141 caagcttGCC ACCATGGTCG ACTCATCACG TCGTAAGTGG AATAAGACAG GTCACGCAGT 1201 CAGAGCTATA GGTCGGCTGA GCTCACTCGA GAACGTCTAT ATCAAGGCCG ACAAGCAGAA 1261 GAACGGCATC AAGGCGAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT 1321 CGCCTACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA 1381 CCACTACCTG AGCGTGCAGT CCAAACTTTC GAAAGACCCC AACGAGAAGC GCGATCACAT 1441 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA 1501 GGGCGGTACC GGAGGGAGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC 1561 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG 1621 TGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT 1681 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG 1741 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACAT 1801 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA 1861 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA 1921 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACCTGCCG GACCAACTGA CTGAAGAGCA 1981 GATCGCAGAA TTTAAAGAGG AATTCTCCCT ATTTGACAAG GACGGGGATG GGACAATAAC 2041 AACCAAGGAG CTGGGGACGG TGATGCGGTC TCTGGGGCAG AACCCCACAG AAGCAGAGCT 2101 GCAGGACATG ATCAATGAAG TAGATGCCGA CGGTGACGGC ACAATCGACT TCCCTGAGTT 2161 CCTGACAATG ATGGCAAGAA AAATGAAATA CAGGGACACG GAAGAAGAAA TTAGAGAAGC 2221 GTTCGGTGTG TTTGATAAGG ATGGCAATGG CTACATCAGT GCAGCAGAGC TTCGCCACGT 2281 GATGACAAAC CTTGGAGAGA AGTTAACAGA TGAAGAGGTT GATGAAATGA TCAGGGAAGC 2341 AGACATCGAT GGGGATGGTC AGGTAAACTA CGAAGAGTTT GTACAAATGA TGACAGCGAA 2401 Ggaattcgga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga 2461 gaaccctgga cctgatatcg ccaccATGTT GTTTCCAAGC AACACTATCT TGGGGGTTTT 2521 CGTCATATCT GAGTTTTGTG TGGGAGTCTT AGGGAACTCA TTGCTCCTCA TGCTGTATAT 2581 ATTCAACTTC TGGGTCAAAG CTCGCTTTAA CAGACAGATC GATCCCATTT TCATGCACCT 2641 GATGATAGTC AATATGTTGA CAATCATATT TGCTATGATA CCATATATCA CATCATCCTT 2701 TGGAGTGCCT AGTTTTCTGC ATGATGCTGG CTGTAAGGCC TTTGTGTATA TCTATAGAGT 2761 CTCCCGGGCT ATGTCCATCT CTAGCACTTC TATTCTAAGC ACATTCCAGG CCATCACCAT 2821 CACTCCCAGT AATTCTAAGT GGGCGTGGCT TAAGCCTAAA CTCTCTAAGT GGACTTTTTG 2881 CACCTTCCTG TTTTCCTGGT TCATCAACAT GGTCATATAT GTGCACCTGA TTGAGAATGT 2941 AATAGCAAAA ACTAATTACA CTGGTGTTAA TTATGGATAT TCTCATGTTT ACTGTGAAAG 3001 TAGTCCAGTT GAGTACCCCA ATCCAGGATT TTTCCTCGGC GTTATCATAA CAAGAGACCT 3061 TGTCCTTCTG GTCATCATGA TGTGGACCAG CCTCTCCATG GTGACCCTCC TCTACAGGCA 3121 TCACAAGAGA GCCCAGCATC TCCGCAACCC AAGACTCTCC TCTCAGAAGT CTCCAGAACA 3181 CAAAGCCACT CACACAATCC TGTTGCTTGT AAACTGCTTT GTGTTCTTTT ATTTGTTGAA 3241 CAACTTAATC ACCCTTTATG ATAGGTTTTA TACACGTGGA AGAACAGGAA ATCTGGGGGC 3301 AATTATCACA ATTGTGGCAT CATTTTACCC TACTCTCTGC CCATTTTTAC TAATGAACAG 3361 TAATAAAATG TATTTCTTCC CTTCCTATAT TCAAAGAGCC ATTGGTGACT Gatccagaca 3421 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 3481 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 3541 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg 3601 ttttttagtc gactagagct cgctgatcag cctcgactgt gccttctagt tgccagccat 3661 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 3721 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 3781 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 3841 gggagagatc tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca 3901 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc 3961 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc 4021 tgatgctgca gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 4081 gttgcgtagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc 4141 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 4201 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 4261 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 4321 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 4381 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 4441 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 4501 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac 4561 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg 4621 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 4681 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 4741 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 4801 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 4861 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 4921 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 4981 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 5041 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 5101 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 5161 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 5221 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 5281 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 5341 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 5401 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 5461 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 5521 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 5581 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 5641 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 5701 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 5761 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 5821 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 5881 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 5941 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 6001 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 6061 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 6121 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 6181 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 6241 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 6301 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 6361 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 6421 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 6481 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 6541 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 6601 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 6661 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 6721 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 6781 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 6841 ccgattcatt aatgcagctg ggctgcaggc tcacatgt //