LOCUS       pTR-UF-12.1_new         6023 bp    DNA     circular      4-JUN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|699884785|
COMMENT     VNTDBDATE|699884996|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTR-UF-12.1_new|
COMMENT     VNTAUTHORNAME|Mingjie Li|
COMMENT     VNTAUTHORTEL|314-747-1123|
COMMENT     VNTAUTHOREML|lim@neuro.wustl.edu|
COMMENT     VNTAUTHORAD1|Department of Neurology|
COMMENT     VNTAUTHORAD2|Washington University School of Medicine|
COMMENT     VNTAUTHORAD3|Campus Box 8111, 660 S. Euclid Ave.|
COMMENT     VNTAUTHORAD4|Saint Louis, MO 63110|
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FEATURES             Location/Qualifiers
     repeat_region   24..156
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     polyA_site      3729..3965
                     /dnas_title="bGHpolyA"
                     /vntifkey="26"
                     /label=bGHpolyA
                     /note="bGHpolyA"
     repeat_region   4000..4121
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     rep_origin      complement(4217..4676)
                     /dnas_title="f1+"
                     /vntifkey="33"
                     /label=f1+
                     /note="f1+"
     exon            complement(5080..6080)
                     /dnas_title="ApR"
                     /vntifkey="61"
                     /label=ApR
                     /note="Ampicillin Resistance"
     rep_origin      complement(6330..6555)
                     /dnas_title="ColE1-ori"
                     /vntifkey="33"
                     /label=ColE1-ori
                     /note="ColE1-ori"
     misc_feature    complement(3731..3754)
                     /dnas_title="AAV-R1 primer"
                     /vntifkey="21"
                     /label=AAV-R1 primer
     source          1..6996
                     /dnas_title="pTR-UF-12-new-gcamp5g-p2a-vmn1r89"
     misc_feature    174..537
                     /dnas_title="CMV Enhancer"
                     /vntifkey="21"
                     /label=CMV Enhancer
     misc_feature    173..1115
                     /note="CMV Enhancer & Chick b-actin promoter & SD &
                     intron in 5' UTR"
     promoter        538..820
                     /dnas_title="Chick B-actin promoter"
                     /vntifkey="29"
                     /label=Chick B-actin promoter
                     /note="Chicken B-actin Promoter"
     misc_feature    913..1019
                     /dnas_title="intron in 5'UTR"
                     /vntifkey="21"
                     /label=intron in 5'UTR
     misc_feature    912..913
                     /dnas_title="SD"
                     /vntifkey="21"
                     /label=SD
                     /note="Splicing donor"
     misc_feature    1065..1066
                     /dnas_title="SA"
                     /vntifkey="21"
                     /label=SA
                     /note="Splicing acceptor"
     misc_feature    1023..1047
                     /dnas_title="AAV-F1 primer"
                     /vntifkey="21"
                     /label=AAV-F1 primer
     misc_feature    5120..5980
                     /note="AmpR"
     misc_feature    3989..4132
                     /note="ITR"
     misc_feature    6997..7140
                     /note="ITR"
     misc_feature    1142..1147
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    1142..1147
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    1142..1147
                     /note="MCS"
     polyA_site      3533..3728
                     /dnas_title="SV40 polyA"
                     /vntifkey="26"
                     /label=SV40 polyA
                     /note="SV40 polyA"
     misc_feature    1154..2503
                     /note="GCaMP5g"
     PCR_primer      2131..2151
                     /dnas_title="TGGGACAATAACAACCAAGGA"
                     /pair=""
                     /primer="TGGGACAATAACAACCAAGGA"
                     /current=0
     PCR_primer      2242..2263
                     /dnas_title="CACAATCGACTTCCCTGAGTTC"
                     /pair=""
                     /primer="CACAATCGACTTCCCTGAGTTC"
                     /current=0
     PCR_primer      2501..2502
                     /dnas_title="AA"
                     /pair=""
                     /primer="AA"
                     /current=0
     PCR_primer      2510..2575
                     /dnas_title="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGAC
                     GTGGAGGAGAACCCTGGACCT"
                     /pair=""
                     /primer="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGG
                     AGGAGAACCCTGGACCT"
                     /current=0
     misc_feature    2510..2575
                     /note="P2A"
     source          2581..2581
                     /organism="Mus musculus"
                     /mol_type="genomic DNA"
                     /strain="129X1/SvJ; 129S1/SvImJ; DBA/2J; A/J"
                     /db_xref="taxon:10090"
                     /note="Celera shotgun assembly combines reads from
                     several strains"
     gene            2581..2581
                     /dnas_title="V1re2"
                     /gene="V1re2"
     mRNA            2581..2581
                     /dnas_title="V1re2"
                     /gene="V1re2"
                     /product="vomeronasal receptor V1RE2"
     CDS             2581..2581
                     /dnas_title="V1re2"
                     /gene="V1re2"
                     /codon_start=1
                     /product="vomeronasal receptor V1RE2"
                     /protein_id="AAL47907.1"
                     /db_xref="GI:18558675"
                     /translation="MDLRTMIVGIVLSLQTALGILGNVSLLFYYLIIYYNEHTMKKID
                     MILIHVFTSNFLTILSKGLPEVVKAFGWKQLFSDVGCKLTNYFQRLARSMSISTTCFL
                     SVFQAITISPRNSCLKEYKTKTTKFMGLFFSLCWILFILVNMLFPIYTATNRNKKNKT
                     QKRDFEFCHSIGRDKLVDIMYTAFCVFPEVIFSLLIVSSSAFMIFILYGHKKRVQHTL
                     HIHASIRNSAENRATHNILTLVCTFLAFYTLSSILQGYIALSNNPSWWVMNITAIISM
                     CFPTLGPIVISPDFAISRFCFT"
     misc_feature    2581..2581
                     /note="V1re2"
     misc_feature    2582..3532
                     /note="v1rj2"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagatc tgaattcggt accctagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      601 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
      661 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      721 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      781 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga cgctgccttc
      841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagc
     1021 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1081 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgaagatc cgaaggggtt
     1141 caagcttGCC ACCATGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG
     1201 TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCTCG CCACCATGGT
     1261 CGACTCATCA CGTCGTAAGT GGAATAAGAC AGGTCACGCA GTCAGAGCTA TAGGTCGGCT
     1321 GAGCTCACTC GAGAACGTCT ATATCAaGGC CGACAAGCAG AAGAACGGCA TCAAGGCGAA
     1381 CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCTACC ACTACCAGCA
     1441 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCGTGCA
     1501 GTCCAAACTT TCGAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
     1561 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGGTA CCGGAGGGAG
     1621 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     1681 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGTGAGGGCG ATGCCACCTA
     1741 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     1801 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
     1861 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC ATCCAGGAGC GCACCATCTT
     1921 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     1981 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     2041 CAAGCTGGAG TACAACCTGC CGGACCAACT GACTGAAGAG CAGATCGCAG AATTTAAAGA
     2101 GGCTTTCTCC CTATTTGACA AGGACGGGGA TGGGACAATA ACAACCAAGG AGCTGGGGAC
     2161 GGTGATGCGG TCTCTGGGGC AGAACCCCAC AGAAGCAGAG CTGCAGGACA TGATCAATGA
     2221 AGTAGATGCC GACGGTGACG GCACAATCGA CTTCCCTGAG TTCCTGACAA TGATGGCAAG
     2281 AAAAATGAAA TACACAGACA GTGAAGAAGA AATTAGAGAA GCGTTCCGTG TGTTTGATAA
     2341 GGATGGCAAT GGCTACATCA GTGCAGCAGA GCTTCGCCAC GTGATGACAA ACCTTGGAGA
     2401 GAAGTTAACA GATGAAGAGG TTGATGAAAT GATCAGGGAA GCAGACATCG ATGGGGATGG
     2461 TCAGGTAAAC TACGAAGAGT TTGTACAAAT GATGACAGCG AAGgaattcg gaagcggagc
     2521 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc
     2581 catgttttca agtgacacat tcttccagat ctttctcata ttccagtttt gtcttggtgt
     2641 gatagggaac tcatcactgt taatgttgta tgtatacact ttcttcttca agcctcattt
     2701 taaaaagttg gtagattcag ttttcatgca cctgacaata gttaatatgt tgacaatcgc
     2761 attcacattg ataaaagata tcatgttatc ctttggagta ccaaattttc tggatgatat
     2821 cggttgtaag acagttttgt tttcattcag agtcagccgg ggtctgtcca tctgcaccac
     2881 ctctgttcta agcacgttcc aagtcatcac catcactccc agtaactcta agtgggcctc
     2941 ctggctcaag cctagactct ctacatggat tttttcttcc ttactttgct cctggcttat
     3001 taacctagtc atctacgggt atatgcttga catggtaata gccaaaacca ataaaaccca
     3061 tgttggcaat ggatattcag atggttactg tcaaaacaaa cactttggga accacaattc
     3121 agggtcattt tttagccttc tcctcactca cgatctcttc tatgtggctg tcatggtgtg
     3181 ggccagtctc tacatggtga tttttctcta cagacactac aagagatccc agcatctcca
     3241 cagtagaagc ctttcctccc agtcatctcc tgagcgcaaa gccactcata acatcctgtc
     3301 tctggtgagc tgctttgtgt taatttattg cttgaacaac tccatcaccc tttatggctt
     3361 ttatacaaaa gaaaaaattc caagattgga gtcaattaat gcaattttaa caacatgcta
     3421 cccaactgtc tgtccttttt tcctcatgaa gaataataaa gttattttgc aattcacttc
     3481 ttcattttcg gtactgagga tgacatgtca tcaaagttca ctgcatggct gacagacatg
     3541 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3601 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3661 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     3721 ttttagtcga ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3781 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3841 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3901 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3961 gagagatctc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4021 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4081 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4141 atgctgcagc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4201 tgcgtagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg
     4261 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4321 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4381 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4441 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4501 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4561 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4621 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4681 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     4741 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4801 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4861 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4921 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4981 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5041 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5101 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5161 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5221 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5281 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5341 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5401 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5461 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5521 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5581 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5641 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5701 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5761 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5821 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5881 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5941 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6001 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6061 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6121 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6181 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6241 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6301 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6361 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6421 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6481 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6541 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6601 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6661 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6721 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6781 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6841 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6901 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6961 gattcattaa tgcagctggg ctgcaggctc acatgt
//

