LOCUS       pTR-UF-12.1_new         6023 bp    DNA     circular      4-JUN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|699884785|
COMMENT     VNTDBDATE|699884996|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTR-UF-12.1_new|
COMMENT     VNTAUTHORNAME|Mingjie Li|
COMMENT     VNTAUTHORTEL|314-747-1123|
COMMENT     VNTAUTHOREML|lim@neuro.wustl.edu|
COMMENT     VNTAUTHORAD1|Department of Neurology|
COMMENT     VNTAUTHORAD2|Washington University School of Medicine|
COMMENT     VNTAUTHORAD3|Campus Box 8111, 660 S. Euclid Ave.|
COMMENT     VNTAUTHORAD4|Saint Louis, MO 63110|
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FEATURES             Location/Qualifiers
     repeat_region   24..156
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     polyA_site      3554..3790
                     /dnas_title="bGHpolyA"
                     /vntifkey="26"
                     /label=bGHpolyA
                     /note="bGHpolyA"
     repeat_region   3825..3946
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     rep_origin      complement(4042..4501)
                     /dnas_title="f1+"
                     /vntifkey="33"
                     /label=f1+
                     /note="f1+"
     exon            complement(4905..5905)
                     /dnas_title="ApR"
                     /vntifkey="61"
                     /label=ApR
                     /note="Ampicillin Resistance"
     rep_origin      complement(6155..6380)
                     /dnas_title="ColE1-ori"
                     /vntifkey="33"
                     /label=ColE1-ori
                     /note="ColE1-ori"
     misc_feature    complement(3556..3579)
                     /dnas_title="AAV-R1 primer"
                     /vntifkey="21"
                     /label=AAV-R1 primer
     source          1..6821
                     /dnas_title="pTR-UF-12.1_new"
     misc_feature    174..537
                     /dnas_title="CMV Enhancer"
                     /vntifkey="21"
                     /label=CMV Enhancer
     misc_feature    173..1115
                     /note="CMV Enhancer & Chick b-actin promoter & SD &
                     intron in 5' UTR"
     promoter        538..820
                     /dnas_title="Chick B-actin promoter"
                     /vntifkey="29"
                     /label=Chick B-actin promoter
                     /note="Chicken B-actin Promoter"
     misc_feature    913..1019
                     /dnas_title="intron in 5'UTR"
                     /vntifkey="21"
                     /label=intron in 5'UTR
     misc_feature    912..913
                     /dnas_title="SD"
                     /vntifkey="21"
                     /label=SD
                     /note="Splicing donor"
     misc_feature    1065..1066
                     /dnas_title="SA"
                     /vntifkey="21"
                     /label=SA
                     /note="Splicing acceptor"
     misc_feature    1023..1047
                     /dnas_title="AAV-F1 primer"
                     /vntifkey="21"
                     /label=AAV-F1 primer
     misc_feature    4945..5805
                     /note="AmpR"
     misc_feature    3814..3957
                     /note="ITR"
     misc_feature    6822..6965
                     /note="ITR"
     misc_feature    1142..1147
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    1142..1147
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    1142..1147
                     /note="MCS"
     polyA_site      3358..3553
                     /dnas_title="SV40 polyA"
                     /vntifkey="26"
                     /label=SV40 polyA
                     /note="SV40 polyA"
     misc_feature    1154..2401
                     /note="G6f"
     PCR_primer      2408..2473
                     /dnas_title="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGAC
                     GTGGAGGAGAACCCTGGACCT"
                     /pair=""
                     /primer="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGG
                     AGGAGAACCCTGGACCT"
                     /current=0
     misc_feature    2408..2473
                     /note="P2A"
     PCR_primer      complement(2450..2473)
                     /dnas_title="AGGTCCAGGGTTCTCCTCCACGTC"
                     /pair=""
                     /primer="AGGTCCAGGGTTCTCCTCCACGTC"
                     /current=0
     PCR_primer      2408..2431
                     /dnas_title="GGAAGCGGAGCTACTAACTTCAGC"
                     /pair=""
                     /primer="GGAAGCGGAGCTACTAACTTCAGC"
                     /current=0
     misc_feature    complement(2480..2485)
                     /note="kozak"
     PCR_primer      2475..2485
                     /dnas_title="GCCACCATGCCAGAGCCAGCGAA"
                     /pair=""
                     /primer="GCCACCATGCCAGAGCCAGCGAA"
                     /current=1
     misc_feature    2486..3355
                     /note="vmn1r237"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagatc tgaattcggt accctagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      601 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
      661 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      721 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      781 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga cgctgccttc
      841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagc
     1021 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1081 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgaagatc cgaaggggtt
     1141 caagcttGCC ACCATGGTCG ACTCATCACG TCGTAAGTGG AATAAGACAG GTCACGCAGT
     1201 CAGAGCTATA GGTCGGCTGA GCTCACTCGA GAACGTCTAT ATCAAGGCCG ACAAGCAGAA
     1261 GAACGGCATC AAGGCGAACT TCAAGATCCG CCACAACATC GAGGACGGCG GCGTGCAGCT
     1321 CGCCTACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
     1381 CCACTACCTG AGCGTGCAGT CCAAACTTTC GAAAGACCCC AACGAGAAGC GCGATCACAT
     1441 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
     1501 GGGCGGTACC GGAGGGAGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC
     1561 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG
     1621 TGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT
     1681 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG
     1741 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACAT
     1801 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA
     1861 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA
     1921 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACCTGCCG GACCAACTGA CTGAAGAGCA
     1981 GATCGCAGAA TTTAAAGAGG AATTCTCCCT ATTTGACAAG GACGGGGATG GGACAATAAC
     2041 AACCAAGGAG CTGGGGACGG TGATGCGGTC TCTGGGGCAG AACCCCACAG AAGCAGAGCT
     2101 GCAGGACATG ATCAATGAAG TAGATGCCGA CGGTGACGGC ACAATCGACT TCCCTGAGTT
     2161 CCTGACAATG ATGGCAAGAA AAATGAAATA CAGGGACACG GAAGAAGAAA TTAGAGAAGC
     2221 GTTCGGTGTG TTTGATAAGG ATGGCAATGG CTACATCAGT GCAGCAGAGC TTCGCCACGT
     2281 GATGACAAAC CTTGGAGAGA AGTTAACAGA TGAAGAGGTT GATGAAATGA TCAGGGAAGC
     2341 AGACATCGAT GGGGATGGTC AGGTAAACTA CGAAGAGTTT GTACAAATGA TGACAGCGAA
     2401 Ggaattcgga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     2461 gaaccctgga cctgatatcg ccaccATGGA AAACAGTGAC ATTGTAATTA GTGTGATCTT
     2521 CTTATCACAG ACTGTAGTTG GGATCCTGGG CAACTCCTTC TTAGTCTACC ATTATCTGAT
     2581 GCTTTACTAC ATGAGGTGCA GACTAAAGTT CACAGACTGG ATTCTGCAGC ACTTGATTGT
     2641 AGCCAACTTC TTAACTCTAC TGTGTAAAGG AATCCCCCAT ACTGTGTCTG CCTTTGGGTT
     2701 GAAAAACTTC CTCGATGACA TTGGTTGTAA ACTGACCTTC TATCTTCACA GAATAGGGAG
     2761 GGGTGTTTCC ATCAGCAGCA CCTGCTTTCT GAGTGTCTTC CAGGCCATCA CCATCAGTCC
     2821 AAGTGCTTCC AGGTGGACAC AGCTTAAAGC CAAGTCTCCC AGTTACATTG CTTCCTGTGT
     2881 GTACATGAGT TGGGCCCTGT CATTCTTAGT AAATATAGCT TTTCCCATGG ACATGAGAGC
     2941 AAGACAAAAC AATAGGAACA TTACACGCCT AAGGGTGTAT GGTATCTGTT CTACTGTTCA
     3001 TCATAACAAG GTCAGTGACA TTATCAATGC AGTATTACTC TCTATTCCTG ATGTGGTGTT
     3061 CATGGTGCTG ATGGTTTGGT CCAGTGGCTA CATGGTTTGT ATTCTTTACA GACATAAGCA
     3121 GAGGATGAAG CACATTCATA GGAGTACCTT TTCATTCAGG TCCTCTGCTG AGTTTAGAGC
     3181 CACAAAGACC ATTCTGCTCT TGGTGAGCAC CTTTGTCTTT TTTTACACAA TTTCTTGCCT
     3241 CTTCCAAATT AATTTGGCTC TTTTCCATAA CCCAACCTCA TTATGGGTGA ACATGGCTTC
     3301 AGTAGTTACT GCATGTTTCC CAACTGTGAG TCCCTTTCTG GTGATCTCCA GTTAAtCcag
     3361 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3421 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3481 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     3541 aggtttttta gtcgactaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     3601 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3661 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3721 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3781 ctggggagag atctcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3841 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3901 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3961 gcctgatgct gcagccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4021 acagttgcgt agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc
     4081 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4141 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4201 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4261 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4321 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4381 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4441 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4501 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4561 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4621 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4681 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     4741 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4801 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5161 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5341 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6121 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6361 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6781 tggccgattc attaatgcag ctgggctgca ggctcacatg t
//

