LOCUS       pTR-UF-12.1_new         6023 bp    DNA     circular      4-JUN-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|699884785|
COMMENT     VNTDBDATE|699884996|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTR-UF-12.1_new|
COMMENT     VNTAUTHORNAME|Mingjie Li|
COMMENT     VNTAUTHORTEL|314-747-1123|
COMMENT     VNTAUTHOREML|lim@neuro.wustl.edu|
COMMENT     VNTAUTHORAD1|Department of Neurology|
COMMENT     VNTAUTHORAD2|Washington University School of Medicine|
COMMENT     VNTAUTHORAD3|Campus Box 8111, 660 S. Euclid Ave.|
COMMENT     VNTAUTHORAD4|Saint Louis, MO 63110|
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FEATURES             Location/Qualifiers
     repeat_region   24..156
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     polyA_site      3717..3953
                     /dnas_title="bGHpolyA"
                     /vntifkey="26"
                     /label=bGHpolyA
                     /note="bGHpolyA"
     repeat_region   3988..4109
                     /dnas_title="ITR"
                     /vntifkey="34"
                     /label=ITR
                     /note="ITR"
     rep_origin      complement(4205..4664)
                     /dnas_title="f1+"
                     /vntifkey="33"
                     /label=f1+
                     /note="f1+"
     exon            complement(5068..6068)
                     /dnas_title="ApR"
                     /vntifkey="61"
                     /label=ApR
                     /note="Ampicillin Resistance"
     rep_origin      complement(6318..6543)
                     /dnas_title="ColE1-ori"
                     /vntifkey="33"
                     /label=ColE1-ori
                     /note="ColE1-ori"
     misc_feature    complement(3719..3742)
                     /dnas_title="AAV-R1 primer"
                     /vntifkey="21"
                     /label=AAV-R1 primer
     source          1..6984
                     /dnas_title="pTR-UF-12-new-gcamp5g-p2a-vmn1r58"
     misc_feature    174..537
                     /dnas_title="CMV Enhancer"
                     /vntifkey="21"
                     /label=CMV Enhancer
     misc_feature    173..1115
                     /note="CMV Enhancer & Chick b-actin promoter & SD &
                     intron in 5' UTR"
     promoter        538..820
                     /dnas_title="Chick B-actin promoter"
                     /vntifkey="29"
                     /label=Chick B-actin promoter
                     /note="Chicken B-actin Promoter"
     misc_feature    913..1019
                     /dnas_title="intron in 5'UTR"
                     /vntifkey="21"
                     /label=intron in 5'UTR
     misc_feature    912..913
                     /dnas_title="SD"
                     /vntifkey="21"
                     /label=SD
                     /note="Splicing donor"
     misc_feature    1065..1066
                     /dnas_title="SA"
                     /vntifkey="21"
                     /label=SA
                     /note="Splicing acceptor"
     misc_feature    1023..1047
                     /dnas_title="AAV-F1 primer"
                     /vntifkey="21"
                     /label=AAV-F1 primer
     misc_feature    5108..5968
                     /note="AmpR"
     misc_feature    3977..4120
                     /note="ITR"
     misc_feature    6985..7128
                     /note="ITR"
     misc_feature    1142..1147
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    1142..1147
                     /dnas_title="MCS"
                     /vntifkey="21"
                     /label=MCS
     misc_feature    1142..1147
                     /note="MCS"
     polyA_site      3521..3716
                     /dnas_title="SV40 polyA"
                     /vntifkey="26"
                     /label=SV40 polyA
                     /note="SV40 polyA"
     misc_feature    1154..2503
                     /note="GCaMP5g"
     PCR_primer      2131..2151
                     /dnas_title="TGGGACAATAACAACCAAGGA"
                     /pair=""
                     /primer="TGGGACAATAACAACCAAGGA"
                     /current=0
     PCR_primer      2242..2263
                     /dnas_title="CACAATCGACTTCCCTGAGTTC"
                     /pair=""
                     /primer="CACAATCGACTTCCCTGAGTTC"
                     /current=0
     PCR_primer      2501..2502
                     /dnas_title="AA"
                     /pair=""
                     /primer="AA"
                     /current=0
     PCR_primer      2510..2575
                     /dnas_title="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGAC
                     GTGGAGGAGAACCCTGGACCT"
                     /pair=""
                     /primer="GGAAGCGGAGCTACTAACTTCAGCCTGCTGAAGCAGGCTGGAGACGTGG
                     AGGAGAACCCTGGACCT"
                     /current=0
     misc_feature    2510..2575
                     /note="P2A"
     PCR_primer      2554..2581
                     /dnas_title="CGTGGAGGAGAACCCTGGACCTGGATCCATGATGAAGCACAGAAT
                     AGACTTC"
                     /pair=""
                     /primer="CGTGGAGGAGAACCCTGGACCTGGATCCATGATGAAGCACAGAATAGAC
                     TTC"
                     /current=0
     source          2581..2581
                     /organism="Mus musculus"
                     /mol_type="genomic DNA"
                     /strain="129X1/SvJ; 129S1/SvImJ; DBA/2J; A/J"
                     /db_xref="taxon:10090"
                     /note="Celera shotgun assembly combines reads from
                     several strains"
     gene            2581..2581
                     /dnas_title="V1re2"
                     /gene="V1re2"
     mRNA            2581..2581
                     /dnas_title="V1re2"
                     /gene="V1re2"
                     /product="vomeronasal receptor V1RE2"
     CDS             2581..2581
                     /dnas_title="V1re2"
                     /gene="V1re2"
                     /codon_start=1
                     /product="vomeronasal receptor V1RE2"
                     /protein_id="AAL47907.1"
                     /db_xref="GI:18558675"
                     /translation="MDLRTMIVGIVLSLQTALGILGNVSLLFYYLIIYYNEHTMKKID
                     MILIHVFTSNFLTILSKGLPEVVKAFGWKQLFSDVGCKLTNYFQRLARSMSISTTCFL
                     SVFQAITISPRNSCLKEYKTKTTKFMGLFFSLCWILFILVNMLFPIYTATNRNKKNKT
                     QKRDFEFCHSIGRDKLVDIMYTAFCVFPEVIFSLLIVSSSAFMIFILYGHKKRVQHTL
                     HIHASIRNSAENRATHNILTLVCTFLAFYTLSSILQGYIALSNNPSWWVMNITAIISM
                     CFPTLGPIVISPDFAISRFCFT"
     misc_feature    2581..2581
                     /note="V1re2"
     misc_feature    2582..3514
                     /note="vmn1r58"
     misc_feature    3514..3514
                     /note=""
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagatc tgaattcggt accctagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      601 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
      661 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      721 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      781 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga cgctgccttc
      841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagc
     1021 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1081 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgaagatc cgaaggggtt
     1141 caagcttGCC ACCATGGGTT CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG
     1201 TGGACAGCAA ATGGGTCGGG ATCTGTACGA CGATGACGAT AAGGATCTCG CCACCATGGT
     1261 CGACTCATCA CGTCGTAAGT GGAATAAGAC AGGTCACGCA GTCAGAGCTA TAGGTCGGCT
     1321 GAGCTCACTC GAGAACGTCT ATATCAaGGC CGACAAGCAG AAGAACGGCA TCAAGGCGAA
     1381 CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCTACC ACTACCAGCA
     1441 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCGTGCA
     1501 GTCCAAACTT TCGAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
     1561 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGCGGTA CCGGAGGGAG
     1621 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     1681 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGTGAGGGCG ATGCCACCTA
     1741 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     1801 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
     1861 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC ATCCAGGAGC GCACCATCTT
     1921 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     1981 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     2041 CAAGCTGGAG TACAACCTGC CGGACCAACT GACTGAAGAG CAGATCGCAG AATTTAAAGA
     2101 GGCTTTCTCC CTATTTGACA AGGACGGGGA TGGGACAATA ACAACCAAGG AGCTGGGGAC
     2161 GGTGATGCGG TCTCTGGGGC AGAACCCCAC AGAAGCAGAG CTGCAGGACA TGATCAATGA
     2221 AGTAGATGCC GACGGTGACG GCACAATCGA CTTCCCTGAG TTCCTGACAA TGATGGCAAG
     2281 AAAAATGAAA TACACAGACA GTGAAGAAGA AATTAGAGAA GCGTTCCGTG TGTTTGATAA
     2341 GGATGGCAAT GGCTACATCA GTGCAGCAGA GCTTCGCCAC GTGATGACAA ACCTTGGAGA
     2401 GAAGTTAACA GATGAAGAGG TTGATGAAAT GATCAGGGAA GCAGACATCG ATGGGGATGG
     2461 TCAGGTAAAC TACGAAGAGT TTGTACAAAT GATGACAGCG AAGgaattcg gaagcggagc
     2521 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc
     2581 cATGGAAATG TTGGCTCTTC AGATCCTTTT GCTTTGCCAT GTTGTGGTTG GGACTGTGGG
     2641 CAACATCCTT CTGTTTGTCC ATAACTTCTC TCCAATCTTG ACTGACTCCA GACTGAAGCC
     2701 AATACAGGTC ATTCTAATCA ACTTGGCTGT GGCCAATGCA TTCATGCTCC TTCTGTTTGC
     2761 ATATTCATAT GACCTTACTG ATATTGTTCC CAGGAAGCCT CCAACTGACC TCAAATGTAA
     2821 ACTTGCATAC TTCTTTCACA TGGTGGCACG AGGCACAATC ATGTGCTCTA CCTGTATCCT
     2881 CAGTACATAC CAGTTTGTCA CTCTTGTCCC TGGTACCTGG GCTAGGGTCA TGTTCAGAGA
     2941 AATATCCCCC AAGGTTGTGA GCTATTGTTG TTGCATCTGC TGGTTGTTCA GTGTCTTAAA
     3001 TAATGCTTAC ATCCCAATGA ATGTCAGTGG TCCACAGAAA TCACACAATG ACTCTGATTC
     3061 TAAAGGCAAT TCAATATGCT CCATCTCTGG GGTCAGTGTA GACATGAATA TCTTGCGGTT
     3121 TTCCCATGAC ATCATATTCC TAAGCATCAT GGCCTGGACC AGTGTCTCCA TGGTGATTCA
     3181 CCTACACAGA CACCACCAGA GAATGAACCA CATACATAAG GCCAATCAAA ACAACAGAGG
     3241 CCATGCTGAG ACCAGAGCAG CCCACACCAT CCTGATGCTG GTGGTCACAT TTGTGAGCTT
     3301 ATATATTCTA AATTGTATTA CTATTTTATT TCACATTTCC TTTGTGGAAT CTCGTCTCTG
     3361 GTTGAGGTAT GCCACAAAAC TCCTGGCTCT AAGCTTCCCC ACCATTTCTC CCTTTCTTTT
     3421 GATATTTAGG GATCGTAAGG GTCATTGTTC TCTGCACATC ATTGTCAGTA TGGGAATCCA
     3481 CGTGACGGGG GGAGCCATTA CAGAGGGACA GTAAGGATCC cagacatgat aagatacatt
     3541 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3601 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3661 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttagtcgact
     3721 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3781 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3841 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3901 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga gagatctcac
     3961 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4021 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4081 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gctgcagcca
     4141 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgtagcctga
     4201 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4261 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4321 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4381 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4441 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4501 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4561 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4621 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcctgatgcg
     4681 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     4741 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4801 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4861 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4921 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5341 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5641 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6241 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     6541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6781 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6841 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6901 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6961 cagctgggct gcaggctcac atgt
//

