LOCUS       Exported                7189 bp ds-DNA     circular SYN 09-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Aleksandar Vjestica
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 9, 2019 from SnapGene 4.3.7
            https://www.snapgene.com

FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label=3''region ura4
                     /note="3''region ura4"
     misc_feature    677..1639
                     /label=5'region ura4
                     /note="5'region ura4"
     CDS             1640..2434
                     /label=ORF ura4
                     /note="ORF ura4"
     misc_feature    2435..2869
                     /label=3'region ura4
                     /note="3'region ura4"
     misc_feature    2926..3381
                     /label=F1 ori
                     /note="F1 ori"
     primer_bind     3521..3538
                     /label=M13 forward
                     /note="M13 forward"
     primer_bind     3548..3567
                     /label=T7
                     /note="T7"
     misc_feature    3575..3705
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     misc_feature    3706..4088
                     /label=Ashbya gossypii TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4089..4664
                     /label=nat ORF
                     /note="nat ORF"
     misc_feature    4665..4897
                     /label=Ashbya gossypii TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4927..4946)
                     /label=T3
                     /note="T3"
     primer_bind     complement(4964..4984)
                     /label=M13 reverse
                     /note="M13 reverse"
     protein_bind    complement(4990..5012)
                     /label=lacO
                     /note="lacO"
     promoter        complement(5016..5046)
                     /label=lac promoter
                     /note="lac promoter"
     rep_origin      complement(5352..5980)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     CDS             complement(6129..6989)
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(6990..7094)
                     /label=AmpR promoter
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcgctt acataatgag tgagtgtcat tattgaggaa tgccatgtca
      721 gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt tttgcccaag
      781 gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc actcttttta
      841 gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg cgtattggat
      901 atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat gagcaaactg
      961 cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat cgcatggaag
     1021 atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa attttgattc
     1081 catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg ctatatgtat
     1141 gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa
     1201 taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt
     1261 atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa
     1321 agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc
     1381 cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt
     1441 tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat
     1501 aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat
     1561 gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta
     1621 aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga
     1681 ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa
     1741 cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat
     1801 tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga
     1861 tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg
     1921 caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat
     1981 tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg
     2041 cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc
     2101 caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca
     2161 taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta
     2221 cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata
     2281 tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg
     2341 agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg
     2401 gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg
     2461 gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca
     2521 gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa
     2581 tattaactat tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt
     2641 aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
     2701 gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt
     2761 agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt
     2821 tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt gctagggcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
     3601 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
     3661 ggccgccacc gcggtggtta attaaggcgc gccagatctg agctcgttta gcttgcctcg
     3721 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     3781 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     3841 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     3901 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     3961 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     4021 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     4081 aaacaaccat gggtaccact cttgacgaca cggcttaccg gtaccgcacc agtgtcccgg
     4141 gggacgccga ggccatcgag gcactggatg ggtccttcac caccgacacc gtcttccgcg
     4201 tcaccgccac cggggacggc ttcaccctgc gggaggtgcc ggtggacccg cccctgacca
     4261 aggtgttccc cgacgacgaa tcggacgacg aatcggacga cggggaggac ggcgacccgg
     4321 actcccggac gttcgtcgcg tacggggacg acggcgacct ggcgggcttc gtggtcatct
     4381 cgtactcggc gtggaaccgc cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc
     4441 gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc ggcgagcggg
     4501 gcgccgggca cctctggctg gaggtcacca acgtcaacgc accggcgatc cacgcgtacc
     4561 ggcggatggg gttcaccctc tgcggcctgg acaccgccct gtacgacggc accgcctcgg
     4621 acggcgagcg gcaggcgctc tacatgagca tgccctgccc ctaatcagta ctgacaataa
     4681 aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct
     4741 attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga
     4801 tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc
     4861 tatactgctg tcgattcgat actaacgccg ccatccacct gcaggtaatc acgtgccagc
     4921 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     4981 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg aagcataaag
     5041 tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg
     5101 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5161 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5221 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5701 gacttatcgc cactggcagc agccactggt aacaggatta gcaaagcgag gtatgtaggc
     5761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6001 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6061 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6121 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6181 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6241 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6301 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6361 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6421 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6481 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6541 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6601 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6661 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6721 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6781 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6841 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6901 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6961 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7021 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7081 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     7141 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
