LOCUS       Exported                7902 bp ds-DNA     circular SYN 09-SEP-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Aleksandar Vjestica
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 9, 2019 from SnapGene 4.3.7
            https://www.snapgene.com

FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label=3''region ura4
                     /note="3''region ura4"
     misc_feature    677..1639
                     /label=5'region ura4
                     /note="5'region ura4"
     CDS             1640..2434
                     /label=ORF ura4
                     /note="ORF ura4"
     misc_feature    2435..2869
                     /label=3'region ura4
                     /note="3'region ura4"
     misc_feature    2926..3381
                     /label=F1 ori
                     /note="F1 ori"
     primer_bind     3521..3538
                     /label=M13 forward
                     /note="M13 forward"
     primer_bind     3548..3567
                     /label=T7
                     /note="T7"
     misc_feature    3575..3580
                     /label=KpnI site
                     /note="KpnI site"
     misc_feature    3581..3586
                     /label=ApaI site
                     /note="ApaI site"
     misc_feature    3587..3594
                     /label=SwaI site
                     /note="SwaI site"
     promoter        3595..4594
                     /label=promoter tdh1
                     /label=Tdh1 Promoter
                     /note="promoter tdh1"
     misc_feature    4595..4600
                     /label=XhoI site
                     /note="XhoI site"
     misc_feature    4601..4606
                     /label=SalI site
                     /note="SalI site"
     misc_feature    4607..4612
                     /label=EcoRI site
                     /note="EcoRI site"
     misc_feature    4613..4620
                     /label=PacI site
                     /note="PacI site"
     CDS             4622..4630
                     /codon_start=1
                     /label=START-Ala-Ser-
                     /note="START-Ala-Ser-"
                     /translation="MAS"
     CDS             4631..5341
                     /codon_start=1
                     /label=sfGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    5342..5347
                     /label=XmaI/SmaI site
                     /note="XmaI/SmaI site"
     misc_feature    5348..5355
                     /label=NotI site
                     /note="NotI site"
     misc_feature    5357..5359
                     /label=STOP codon
                     /note="STOP codon"
     misc_feature    5360..5367
                     /label=AscI site
                     /note="AscI site"
     misc_feature    5368..5617
                     /label=terminator ScCYC1
                     /note="terminator ScCYC1"
     misc_feature    5618..5623
                     /label=BglII site
                     /note="BglII site"
     misc_feature    5624..5629
                     /label=SacI site
                     /note="SacI site"
     primer_bind     complement(5640..5659)
                     /label=T3
                     /note="T3"
     primer_bind     complement(5677..5697)
                     /label=M13 reverse
                     /note="M13 reverse"
     protein_bind    complement(5703..5725)
                     /label=lacO
                     /note="lacO"
     promoter        complement(5729..5759)
                     /label=lac promoter
                     /note="lac promoter"
     rep_origin      complement(6065..6693)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     CDS             complement(6842..7702)
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(7703..7807)
                     /label=AmpR promoter
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcgctt acataatgag tgagtgtcat tattgaggaa tgccatgtca
      721 gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt tttgcccaag
      781 gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc actcttttta
      841 gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg cgtattggat
      901 atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat gagcaaactg
      961 cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat cgcatggaag
     1021 atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa attttgattc
     1081 catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg ctatatgtat
     1141 gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa
     1201 taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt
     1261 atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa
     1321 agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc
     1381 cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt
     1441 tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat
     1501 aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat
     1561 gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta
     1621 aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga
     1681 ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa
     1741 cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat
     1801 tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga
     1861 tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg
     1921 caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat
     1981 tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg
     2041 cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc
     2101 caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca
     2161 taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta
     2221 cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata
     2281 tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg
     2341 agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg
     2401 gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg
     2461 gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca
     2521 gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa
     2581 tattaactat tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt
     2641 aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
     2701 gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt
     2761 agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt
     2821 tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt gctagggcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccattt aaattaaagt
     3601 atggaaaatc aaaattcctg aatttttgta aactcccata caaatacgat tacacataca
     3661 tacaaaaatg caaataacaa aatatacacg cacgcatgca cactaaataa acaaactgtg
     3721 cgtccattcc ttttatgcac aaacgttgaa gctcaggaca tcgcaccaac aatcactcat
     3781 ctcttctctc tctacccact ttccacccac acccacagtc tgcatctgtt tgacgactgt
     3841 agctcaagca tcagtagcag ccgctgtcat cctcaactgc tgctgctgat gctgcttgaa
     3901 tgtacgcatg atagcaacga cgcaccgctc taccactact accgaagcgc catcatcacc
     3961 tcctcaccta ccatcgcaat cgtcctctcg aaactcttcg accgctttca cccggcttcc
     4021 tgcaggtaac aatgcgctcg gcgcttgatt aaaagaggcc agaggcgata aatgactcag
     4081 ggtgcatttg gcctcgctac gaaaggaccc tgtcttgtga tttttccgta gccaatggac
     4141 aaccgccttg tcaacctttt gactttggaa tgtgctagca attcctcctt ccccctgact
     4201 tcaggtgaca aggcttgcat cacgctgagg taggcgtcgg aaaaactttc ctcacaaggc
     4261 tgacgtcatg gaatgtgcaa tcaaagtcag gagcgggaac gaggcagatt accaacgaag
     4321 caacgaccat ctcggaacaa cttgcaaaga aagtcaagga aagttggcac cacatcacga
     4381 tcgcgcacct tctccaatca ccttgctcct tagttgttcc gaatcgagtg cgaggcaatt
     4441 gtggtaaaga tagtcggggg aaacccttac cccattcaca ccacggctga tggctgctgt
     4501 gtgggatgga aaggtataaa tagaggcagg cggtgcctag catcgcttct gtatagatca
     4561 ttcatccata gtattgattt acacttgatt caaactcgag gtcgacgaat tcttaattaa
     4621 catggcaagc tccaagggtg aagagctatt tactggggtt gtacccattt tggtagaact
     4681 ggacggagat gtaaacggac ataaattctc tgttagaggt gagggcgaag gcgatgccac
     4741 caatggtaaa ttgactctga agtttatatg cactacgggt aaattacctg ttccttggcc
     4801 aaccctagta acaactttga catatggtgt tcaatgtttc tcaagatacc cagaccatat
     4861 gaaaaggcat gatttcttta aaagtgctat gccagaaggc tacgtgcaag agagaactat
     4921 ctcctttaag gatgacggta cgtataaaac acgagcagaa gtgaaattcg aaggggatac
     4981 actagttaat cgcatcgaat taaagggtat agactttaag gaagatggta atattctcgg
     5041 ccataaactt gagtataatt tcaactcgca taatgtgtac attacagctg acaaacaaaa
     5101 gaacggaatt aaagcgaatt ttaaaatcag gcacaacgtc gaagatgggt ctgttcaact
     5161 tgccgatcat tatcagcaaa acacccctat tggtgatggt ccagtcttgt tacccgataa
     5221 tcactactta agcacacagt ctagattgtc aaaagatccg aatgaaaagc gtgatcacat
     5281 ggttttattg gaatttgtca ccgctgcagg aataactcac ggaatggacg agctttataa
     5341 gcccggggcg gccgcttaag gcgcgcctca tgtaattagt tatgtcacgc ttacattcac
     5401 gccctcctcc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     5461 tccctattta ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat
     5521 ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct
     5581 tgagaaggtt ttgggacgct cgaaggcttt aatttgcaga tctgagctcc agcttttgtt
     5641 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5701 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5761 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5821 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5881 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5941 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6421 cgccactggc agcagccact ggtaacagga ttagcaaagc gaggtatgta ggcggtgcta
     6481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6841 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6901 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6961 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7021 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7081 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7141 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7201 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7261 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7321 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7381 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7441 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7501 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7561 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7621 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7681 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7741 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7801 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     7861 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
