LOCUS       Exported                8072 bp ds-DNA     circular SYN 09-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Aleksandar Vjestica
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 9, 2019 from SnapGene 4.3.7
            https://www.snapgene.com

FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label=3''region ura4
                     /note="3''region ura4"
     misc_feature    677..1639
                     /label=5'region ura4
                     /note="5'region ura4"
     CDS             1640..2434
                     /label=ORF ura4
                     /note="ORF ura4"
     misc_feature    2435..2869
                     /label=3'region ura4
                     /note="3'region ura4"
     misc_feature    2926..3381
                     /label=F1 ori
                     /note="F1 ori"
     primer_bind     3521..3538
                     /label=M13 forward
                     /note="M13 forward"
     primer_bind     3548..3567
                     /label=T7
                     /note="T7"
     misc_feature    3575..3580
                     /label=KpnI site
                     /note="KpnI site"
     misc_feature    3581..3586
                     /label=ApaI site
                     /note="ApaI site"
     misc_feature    3587..3594
                     /label=SwaI site
                     /note="SwaI site"
     promoter        3595..4764
                     /label=promoter nmt81
                     /note="pnmt81 promoter"
     misc_feature    4765..4770
                     /label=XhoI site
                     /note="XhoI site"
     misc_feature    4771..4776
                     /label=SalI site
                     /note="SalI site"
     misc_feature    4777..4782
                     /label=EcoRI site
                     /note="EcoRI site"
     misc_feature    4783..4790
                     /label=PacI site
                     /note="PacI site"
     CDS             4792..4800
                     /codon_start=1
                     /label=START-Ala-Ser-
                     /note="START-Ala-Ser-"
                     /translation="MAS"
     CDS             4801..5511
                     /codon_start=1
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    5512..5517
                     /label=XmaI/SmaI site
                     /note="XmaI/SmaI site"
     misc_feature    5518..5525
                     /label=NotI site
                     /note="NotI site"
     misc_feature    5527..5529
                     /label=STOP codon
                     /note="STOP codon"
     misc_feature    5530..5537
                     /label=AscI site
                     /note="AscI site"
     misc_feature    5538..5787
                     /label=terminator ScCYC1
                     /note="terminator ScCYC1"
     misc_feature    5788..5793
                     /label=BglII site
                     /note="BglII site"
     misc_feature    5794..5799
                     /label=SacI site
                     /note="SacI site"
     primer_bind     complement(5810..5829)
                     /label=T3
                     /note="T3"
     primer_bind     complement(5847..5867)
                     /label=M13 reverse
                     /note="M13 reverse"
     protein_bind    complement(5873..5895)
                     /label=lacO
                     /note="lacO"
     promoter        complement(5899..5929)
                     /label=lac promoter
                     /note="lac promoter"
     rep_origin      complement(6235..6863)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     CDS             complement(7012..7872)
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(7873..7977)
                     /label=AmpR promoter
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcgctt acataatgag tgagtgtcat tattgaggaa tgccatgtca
      721 gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt tttgcccaag
      781 gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc actcttttta
      841 gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg cgtattggat
      901 atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat gagcaaactg
      961 cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat cgcatggaag
     1021 atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa attttgattc
     1081 catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg ctatatgtat
     1141 gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa
     1201 taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt
     1261 atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa
     1321 agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc
     1381 cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt
     1441 tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat
     1501 aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat
     1561 gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta
     1621 aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga
     1681 ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa
     1741 cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat
     1801 tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga
     1861 tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg
     1921 caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat
     1981 tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg
     2041 cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc
     2101 caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca
     2161 taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta
     2221 cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata
     2281 tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg
     2341 agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg
     2401 gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg
     2461 gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca
     2521 gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa
     2581 tattaactat tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt
     2641 aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
     2701 gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt
     2761 agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt
     2821 tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt gctagggcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccattt aaatgatcag
     3601 aaaattatcg ccataaaaga cagaataagt catcagcggt tgtttcattt cctatatttt
     3661 ttttttattt ttttattttt taataaggga aaatttaacg tctaaggata cagaagattg
     3721 ttagcacatt aaagtaataa aggcttaagt agtaagtgcc ttagcatgtt attgtatttc
     3781 aaaggacata atctaaaata ataacaatat catttctcac aagttattca attttctttt
     3841 ttttttctaa taatatcaag aatgtattat ttgtttgaca taagtcaact aatttattta
     3901 atatgctgga ttaatcttgc agacatgtaa attaacaagt tttagtcaaa taacgttgaa
     3961 gtttcaatga actcaaataa tttctctttt tttttatata accatatgtc taatctgatt
     4021 tatattttcc gcagggatca actgaagtta tgacatttgg attggatcac ttataacctt
     4081 ggtcgccaaa taatacaaaa atcagcgtta taaaacaaag aaggtttttg ttaagaaatt
     4141 aatcctcttt cttgataaga aagttgaacc gaaattgcag atactgatat atgaaaataa
     4201 tacccacaat tttgggaata gcgcaagcct caatttaaac aataggtgag gacacatgat
     4261 aatgacctca atgattgtta gaagaaaaga gcctcattac aaaatcgaaa aatgaatggt
     4321 tgggtacaag tttccaaaac atggtaaagt ggactttgcg tatgagacgt aaatagaaaa
     4381 aaacacttgt tatatgtttt ctagaattat tgttgtctct ttatggttgg atgatgcaaa
     4441 atagtaattt cggttagttg ctgtaaaaca ccacgagaca aatagatatg gatatttatt
     4501 aaatcaggaa aaacgtaact ctcggctact ggatggttca gtcacccaac gattactggg
     4561 gagagaaaac agggcaaaag caaagcttaa aggaatccga ttgtcattcg gcaatgtgca
     4621 gcgaaactaa aaaccggata atggacctgt taatcgaaac attgaagatg gaagaggaat
     4681 cctggcatat catcaattga ataagttgaa ttaattattt caatctcatt ctcactttct
     4741 gacttatagt cgctttgtta aatcctcgag gtcgacgaat tcttaattaa catggcaagc
     4801 tccaagggtg aagagctatt tactggggtt gtacccattt tggtagaact ggacggagat
     4861 gtaaacggac ataaattctc tgttagaggt gagggcgaag gcgatgccac caatggtaaa
     4921 ttgactctga agtttatatg cactacgggt aaattacctg ttccttggcc aaccctagta
     4981 acaactttga catatggtgt tcaatgtttc tcaagatacc cagaccatat gaaaaggcat
     5041 gatttcttta aaagtgctat gccagaaggc tacgtgcaag agagaactat ctcctttaag
     5101 gatgacggta cgtataaaac acgagcagaa gtgaaattcg aaggggatac actagttaat
     5161 cgcatcgaat taaagggtat agactttaag gaagatggta atattctcgg ccataaactt
     5221 gagtataatt tcaactcgca taatgtgtac attacagctg acaaacaaaa gaacggaatt
     5281 aaagcgaatt ttaaaatcag gcacaacgtc gaagatgggt ctgttcaact tgccgatcat
     5341 tatcagcaaa acacccctat tggtgatggt ccagtcttgt tacccgataa tcactactta
     5401 agcacacagt ctagattgtc aaaagatccg aatgaaaagc gtgatcacat ggttttattg
     5461 gaatttgtca ccgctgcagg aataactcac ggaatggacg agctttataa gcccggggcg
     5521 gccgcttaag gcgcgcctca tgtaattagt tatgtcacgc ttacattcac gccctcctcc
     5581 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     5641 ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     5701 tttctgtaca aacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     5761 ttgggacgct cgaaggcttt aatttgcaga tctgagctcc agcttttgtt ccctttagtg
     5821 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5941 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6001 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6601 agcagccact ggtaacagga ttagcaaagc gaggtatgta ggcggtgcta cagagttctt
     6661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7021 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7081 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7141 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7201 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7261 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7321 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7381 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7441 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7501 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7741 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7981 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     8041 taaaaatagg cgtatcacga ggccctttcg tc
//
