LOCUS       Exported                9735 bp ds-DNA     circular SYN 09-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Aleksandar Vjestica
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 9, 2019 from SnapGene 4.3.7
            https://www.snapgene.com

FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label=3''region ura4
                     /note="3''region ura4"
     misc_feature    677..1639
                     /label=5'region ura4
                     /note="5'region ura4"
     CDS             1640..2434
                     /label=ORF ura4
                     /note="ORF ura4"
     misc_feature    2435..2869
                     /label=3'region ura4
                     /note="3'region ura4"
     misc_feature    2926..3381
                     /label=F1 ori
                     /note="F1 ori"
     primer_bind     3521..3538
                     /label=M13 forward
                     /note="M13 forward"
     primer_bind     3548..3567
                     /label=T7
                     /note="T7"
     misc_feature    3589..4588
                     /label=Tdh1 Promoter
                     /note="Tdh1 Promoter"
     misc_feature    4595..5305
                     /label=mTagBFP2
                     /note="note=""direct strand 
                     translation""translation=""MVSKGEELIKENMHMKLYMEGTVDNHHFKCTS
                      
                     EGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEG
                      
                     FTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTET
                      
                     LYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEA
                      ""3807..4519 /note=""note=""Fragment extracted from 
                     Ligation #1 [713 nt] : (SpeI[3680] / NheI[4397])"
     misc_feature    5312..6022
                     /label=mTagBFP2
                     /note="note=""direct strand 
                     translation""translation=""MVSKGEELIKENMHMKLYMEGTVDNHHFKCTS
                      
                     EGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEG
                      
                     FTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTET
                      
                     LYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEA
                      ""3807..4519 /note=""note=""Fragment extracted from 
                     Ligation #1 [713 nt] : (SpeI[3680] / NheI[4397])"
     misc_feature    6029..6739
                     /label=mTagBFP2
                     /note="note=""direct strand 
                     translation""translation=""MVSKGEELIKENMHMKLYMEGTVDNHHFKCTS
                      
                     EGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEG
                      
                     FTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTET
                      
                     LYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEA
                      ""3807..4519 /note=""note=""Fragment extracted from 
                     Ligation #1 [713 nt] : (SpeI[3680] / NheI[4397])"
     misc_feature    6748..7447
                     /label=terminator SpTdh1
                     /note="terminator SpTdh1"
     primer_bind     complement(7473..7492)
                     /label=T3
                     /note="T3"
     primer_bind     complement(7510..7530)
                     /label=M13 reverse
                     /note="M13 reverse"
     protein_bind    complement(7536..7558)
                     /label=lacO
                     /note="lacO"
     promoter        complement(7562..7592)
                     /label=lac promoter
                     /note="lac promoter"
     rep_origin      complement(7898..8526)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     CDS             complement(8675..9535)
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(9536..9640)
                     /label=AmpR promoter
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcgctt acataatgag tgagtgtcat tattgaggaa tgccatgtca
      721 gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt tttgcccaag
      781 gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc actcttttta
      841 gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg cgtattggat
      901 atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat gagcaaactg
      961 cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat cgcatggaag
     1021 atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa attttgattc
     1081 catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg ctatatgtat
     1141 gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc tttctctaaa
     1201 taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt atttgtttgt
     1261 atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc atggtgaaaa
     1321 agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag ccttaatgcc
     1381 cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt gaatatattt
     1441 tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt ttgtctttat
     1501 aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat ctctgcacat
     1561 gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg atcgaaatta
     1621 aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag ctagagctga
     1681 ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa agcaaagcaa
     1741 cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg tagataaaat
     1801 tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt tcgaccagga
     1861 tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct ttgaggatcg
     1921 caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg tgtacaaaat
     1981 tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta ttatacaagg
     2041 cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg aaatgtcttc
     2101 caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt ttgagaagca
     2161 taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc aaagcgacta
     2221 cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg gacagcaata
     2281 tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg ttggtcgtgg
     2341 agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag aagctggttg
     2401 gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa aatttttttg
     2461 gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg ttttatgtca
     2521 gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta gcgactaaaa
     2581 tattaactat tatagataaa caccttggga ataaaaagta atttgctata gtaatttatt
     2641 aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg aataagaaaa
     2701 gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa agatgtatgt
     2761 agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa acattggtgt
     2821 tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt gctagggcgg
     2881 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     2941 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     3001 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     3061 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     3121 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     3181 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     3241 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     3301 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     3361 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3421 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3481 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3541 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gagcatgcta aagtatggaa
     3601 aatcaaaatt cctgaatttt tgtaaactcc catacaaata cgattacaca tacatacaaa
     3661 aatgcaaata acaaaatata cacgcacgca tgcacactaa ataaacaaac tgtgcgtcca
     3721 ttccttttat gcacaaacgt tgaagctcag gacatcgcac caacaatcac tcatctcttc
     3781 tctctctacc cactttccac ccacacccac agtctgcatc tgtttgacga ctgtagctca
     3841 agcatcagta gcagccgctg tcatcctcaa ctgctgctgc tgatgctgct tgaatgtacg
     3901 catgatagca acgacgcacc gctctaccac tactaccgaa gcgccatcat cacctcctca
     3961 cctaccatcg caatcgtcct ctcgaaactc ttcgaccgct ttcacccggc ttcctgcagg
     4021 taacaatgcg ctcggcgctt gattaaaaga ggccagaggc gataaatgac tcagggtgca
     4081 tttggcctcg ctacgaaagg accctgtctt gtgatttttc cgtagccaat ggacaaccgc
     4141 cttgtcaacc ttttgacttt ggaatgtgct agcaattcct ccttccccct gacttcaggt
     4201 gacaaggctt gcatcacgct gaggtaggcg tcggaaaaac tttcctcaca aggctgacgt
     4261 catggaatgt gcaatcaaag tcaggagcgg gaacgaggca gattaccaac gaagcaacga
     4321 ccatctcgga acaacttgca aagaaagtca aggaaagttg gcaccacatc acgatcgcgc
     4381 accttctcca atcaccttgc tccttagttg ttccgaatcg agtgcgaggc aattgtggta
     4441 aagatagtcg ggggaaaccc ttaccccatt cacaccacgg ctgatggctg ctgtgtggga
     4501 tggaaaggta taaatagagg caggcggtgc ctagcatcgc ttctgtatag atcattcatc
     4561 catagtattg atttacactt gattcaaacc tagtatggtg tctaagggcg aagagctgat
     4621 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     4681 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     4741 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     4801 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     4861 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     4921 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     4981 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     5041 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     5101 gggcgggagc catctgatcg caaacgccaa gaccacatat agatccaaga aacccgctaa
     5161 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     5221 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     5281 tagcaaactg gggcacaagc ttaatgctag tatggtgtct aagggcgaag agctgattaa
     5341 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
     5401 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     5461 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
     5521 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
     5581 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     5641 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     5701 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
     5761 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
     5821 cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa
     5881 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
     5941 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
     6001 caaactgggg cacaagctta atgctagtat ggtgtctaag ggcgaagagc tgattaagga
     6061 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
     6121 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     6181 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
     6241 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
     6301 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     6361 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
     6421 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
     6481 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
     6541 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
     6601 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
     6661 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
     6721 actggggcac aagcttaatg ctagagcttt tatgattttg ataaatactt tttttcgttt
     6781 tggtttgtga tacgtatagt ttgcctgtct ccttttgtaa atgatcttct tttgtagtct
     6841 tgtgtaatct ctttttcatt tggtcaactt aagtgaattt ttttttgtca gtttccttgt
     6901 aggatattat gtcaaagtct tatttatact atatattatt gctttggtgg tgagtcgcat
     6961 ttgtggggac ggtgttactt tactgatgga tttaggtagg aggcatccac tgtcccccat
     7021 ttcgctagct taagtttaat atgaagatgc acttaagcat gaacgcccgt tttgatcttt
     7081 ttcacttgta ccgcgaagag cttgttttac tgtttttccg tctgcatccc gagaactagt
     7141 tgatctccca atgcacactt tagactttat tcaaaccgaa gtcggttttg taatttaaaa
     7201 atttttaccc actgtaaagt acaaacaatt catcgctatg ttagttgtgt acgctactag
     7261 cgcatcacat gattacatgt taatcaacaa aaacgaaaat gtttattgtc aatctaaaaa
     7321 tacatatgtc cgttaagaca aaagtatgag gctggatgga atgagcaatg gtagcaaaag
     7381 gtttccgaaa tggtcatatc attttttcaa tttttgctga tctcaaatac ctcaacttgt
     7441 tttattcgtt taaaccgagc tccagctttt gttcccttta gtgagggtta attgcgcgct
     7501 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8281 ggattagcaa agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8641 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8701 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8761 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8821 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8881 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8941 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9001 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9061 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9121 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9181 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9241 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9301 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9361 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9421 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9481 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9661 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9721 cgaggccctt tcgtc
//
