LOCUS       Exported                8230 bp ds-DNA     circular SYN 15-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Aleksandar Vjestica
  TITLE     Direct Submission
  JOURNAL   Exported Mar 15, 2019 from SnapGene Viewer 4.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pAV0478 
                     pHis5StuI-Ptdh1-NLS-sfGFP-NLS-natMX"
     misc_feature    193..667
                     /label=3''region his5
                     /note="3''region his5"
     misc_feature    668..1177
                     /label=5'region his5
                     /note="5'region his5"
     CDS             1178..1828
                     /label=his5 ORF
                     /note="his5 ORF"
     misc_feature    1829..2206
                     /label=3'region his5
                     /note="3'region his5"
     misc_feature    2262..2717
                     /label=F1 ori
                     /note="F1 ori"
     primer_bind     2857..2874
                     /label=M13 forward
                     /note="M13 forward"
     primer_bind     2884..2903
                     /label=T7
                     /note="T7"
     promoter        2917..3916
                     /note="tdh1 promoter"
     CDS             3936..4730
                     /label=NLS-sfGFP-NLS
                     /note="NLS-sfGFP-NLS"
     misc_feature    3945..3965
                     /label=NLS
                     /note="NLS"
     misc_feature    3969..4676
                     /label=sfGFP
     misc_feature    4680..4700
                     /label=NLS
                     /note="NLS"
     promoter        4792..5134
                     /note="Ashbya gossypii TEF promoter"
     CDS             5135..5710
                     /label=NatMX ORF
                     /note="NatMX ORF"
     terminator      5711..5913
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(5968..5987)
                     /label=T3
                     /note="T3"
     primer_bind     complement(6005..6025)
                     /label=M13 reverse
                     /note="M13 reverse"
     protein_bind    complement(6031..6053)
                     /note="lacO"
     promoter        complement(6057..6087)
                     /note="lac promoter"
     rep_origin      complement(6393..7021)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     CDS             complement(7170..8030)
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(8031..8135)
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggatggaacg attgaatact gtgctactct ttatgtaata taacgaattc
      241 aactgttcaa cgtatgtgac tgctctctgc ttctcggtat atatttactt gaaggaatta
      301 atttttcttt gccaatggtc tcttttacca attgcagtct ggcttaaagc aatgaggttg
      361 taattaaatg gcttgagacc tagaacaaac accttttaca atccttgttt actggttttg
      421 atagtaaatt cggttcgctt gtggtgtaca ttatattttt taggctagtc gtgatttttt
      481 gataatatca atattcttag atttgagaca ttcatgcttt ttaatttgat caatcgtttg
      541 tatcttcatt aattagtagg tggtttgata gaacatttag ttgcttgttt gactacaaat
      601 atctgcagaa tggattcggt taataaaaaa aatgtttgag gaattaattt tcgctttgga
      661 gtggaggcct caattcgatg ttgcagattt tacaagtttt taaaatgtat ttcattatta
      721 ctttttatat gcctaataaa aaagccatag tttaatctat agataacttt ttttccagtg
      781 cactaacgga cgttacattc ccatacaaaa ctgcgtagtt aaagctaagg aaaagttaat
      841 atcatgttaa ttaaatacgc tatttacaat aagacattga actcatttat atcgttgaat
      901 atgaataacc aatttcagcg aatttttaac aaacatcgtt cacctcgttt aaggatatct
      961 tgtgtatggg gtgttgactt gctttatcga ataattaccg tacctgtaat tggcttgctg
     1021 gatatagcgg tagtctaata tctagcaaaa atcttttggg tgaaaaggct tgcaatttca
     1081 cgacaccgaa ctatttgtca ttttttaata aggaagtttt ccataaattc ctgtaattct
     1141 cggttgatct aattgaaaag agtagttttg catcacgatg aggagggctt ttgtagaaag
     1201 aaatacgaac gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc ccttacctga
     1261 agagtcgaat tttattgatg aacttataac ttccaagcat gcaaaccaaa agggagaaca
     1321 agtaatccaa gtagacacgg gaattggatt cttggatcac atgtatcatg cactggctaa
     1381 acatgcaggc tggagcttac gactttactc aagaggtgat ttaatcatcg atgatcatca
     1441 cactgcagaa gatactgcta ttgcacttgg tattgcattc aagcaggcta tgggtaactt
     1501 tgccggcgtt aaaagatttg gacatgctta ttgtccactt gacgaagctc tttctagaag
     1561 cgtagttgac ttgtcgggac ggccctatgc tgttatcgat ttgggattaa agcgtgaaaa
     1621 ggttggggaa ttgtcctgtg aaatgatccc tcacttacta tattcctttt cggtagcagc
     1681 tggaattact ttgcatgtta cctgcttata tggtagtaat gaccatcatc gtgctgaaag
     1741 cgcttttaaa tctctggctg ttgccatgcg cgcggctact agtcttactg gaagttctga
     1801 agtcccaagc acgaagggag tgttgtaaag atgaattgga ttatgtcagg aaaagaacga
     1861 caattttgca tccaaattgt ctaaatttta gagttgcttg aaaacaatag aaccttactt
     1921 gctttataat tacgttaatt agaagcgtta tctcgtgaag gaatatagta cgtagccgta
     1981 taaattgaat tgaatgttca gcttatagaa tagagacact ttgctgttca atgcgtcgtc
     2041 acttaccata ctcactttat tatacgactt taagtataaa ctccgcggtt atggtaaaat
     2101 taatgatgca caaacgtccg attccatatg ggtacactac aattaaatac ttttaagtaa
     2161 cgctgatttc ttatttttta tccttggtgt atttattgtt tataccgcta gggcggtgtg
     2221 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     2281 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2341 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     2401 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     2461 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     2521 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     2581 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     2641 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     2701 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     2761 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     2821 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     2881 gcgtaatacg actcactata gggcgaattg ggtacctaaa gtatggaaaa tcaaaattcc
     2941 tgaatttttg taaactccca tacaaatacg attacacata catacaaaaa tgcaaataac
     3001 aaaatataca cgcacgcatg cacactaaat aaacaaactg tgcgtccatt ccttttatgc
     3061 acaaacgttg aagctcagga catcgcacca acaatcactc atctcttctc tctctaccca
     3121 ctttccaccc acacccacag tctgcatctg tttgacgact gtagctcaag catcagtagc
     3181 agccgctgtc atcctcaact gctgctgctg atgctgcttg aatgtacgca tgatagcaac
     3241 gacgcaccgc tctaccacta ctaccgaagc gccatcatca cctcctcacc taccatcgca
     3301 atcgtcctct cgaaactctt cgaccgcttt cacccggctt cctgcaggta acaatgcgct
     3361 cggcgcttga ttaaaagagg ccagaggcga taaatgactc agggtgcatt tggcctcgct
     3421 acgaaaggac cctgtcttgt gatttttccg tagccaatgg acaaccgcct tgtcaacctt
     3481 ttgactttgg aatgtgctag caattcctcc ttccccctga cttcaggtga caaggcttgc
     3541 atcacgctga ggtaggcgtc ggaaaaactt tcctcacaag gctgacgtca tggaatgtgc
     3601 aatcaaagtc aggagcggga acgaggcaga ttaccaacga agcaacgacc atctcggaac
     3661 aacttgcaaa gaaagtcaag gaaagttggc accacatcac gatcgcgcac cttctccaat
     3721 caccttgctc cttagttgtt ccgaatcgag tgcgaggcaa ttgtggtaaa gatagtcggg
     3781 ggaaaccctt accccattca caccacggct gatggctgct gtgtgggatg gaaaggtata
     3841 aatagaggca ggcggtgcct agcatcgctt ctgtatagat cattcatcca tagtattgat
     3901 ttacacttga ttcaaaccta ggccgcggaa gggggatggg ttcccctaag aagaagcgta
     3961 aggtttccaa gggtgaagag ctatttactg gggttgtacc cattttggta gaactggacg
     4021 gagatgtaaa cggacataaa ttctctgtta gaggtgaggg cgaaggcgat gccaccaatg
     4081 gtaaattgac tctgaagttt atatgcacta cgggtaaatt acctgttcct tggccaaccc
     4141 tagtaacaac tttgacatat ggtgttcaat gtttctcaag atacccagac catatgaaaa
     4201 ggcatgattt ctttaaaagt gctatgccag aaggctacgt gcaagagaga actatctcct
     4261 ttaaggatga cggtacgtat aaaacacgag cagaagtgaa attcgaaggg gatacactag
     4321 ttaatcgcat cgaattaaag ggtatagact ttaaggaaga tggtaatatt ctcggccata
     4381 aacttgagta taatttcaac tcgcataatg tgtacattac agctgacaaa caaaagaacg
     4441 gaattaaagc gaattttaaa atcaggcaca acgtcgaaga tgggtctgtt caacttgccg
     4501 atcattatca gcaaaacacc cctattggtg atggtccagt cttgttaccc gataatcact
     4561 acttaagcac acagtctaga ttgtcaaaag atccgaatga aaagcgtgat cacatggttt
     4621 tattggaatt tgtcaccgct gcaggaataa ctcacggaat ggacgagctt tataaggggc
     4681 ctaagaagaa gcgtaaggtt ggtggtcccg gtggtcatgg atccccgggt taattaaggc
     4741 gcgccagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg
     4801 cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc
     4861 gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata cattttgatg
     4921 gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg
     4981 ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag
     5041 gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat
     5101 acagttctca catcacatcc gaacataaac aaccatgggt accactcttg acgacacggc
     5161 ttaccggtac cgcaccagtg tcccggggga cgccgaggcc atcgaggcac tggatgggtc
     5221 cttcaccacc gacaccgtct tccgcgtcac cgccaccggg gacggcttca ccctgcggga
     5281 ggtgccggtg gacccgcccc tgaccaaggt gttccccgac gacgaatcgg acgacgaatc
     5341 ggacgacggg gaggacggcg acccggactc ccggacgttc gtcgcgtacg gggacgacgg
     5401 cgacctggcg ggcttcgtgg tcatctcgta ctcggcgtgg aaccgccggc tgaccgtcga
     5461 ggacatcgag gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt tgatggggct
     5521 cgcgacggag ttcgccggcg agcggggcgc cgggcacctc tggctggagg tcaccaacgt
     5581 caacgcaccg gcgatccacg cgtaccggcg gatggggttc accctctgcg gcctggacac
     5641 cgccctgtac gacggcaccg cctcggacgg cgagcggcag gcgctctaca tgagcatgcc
     5701 ctgcccctaa tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta
     5761 tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt
     5821 tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc
     5881 gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat
     5941 ccagtttaaa cgagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     6001 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6061 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6181 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6241 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6301 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6361 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6421 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6481 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6541 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6601 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6661 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6721 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6781 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6841 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6901 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6961 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7021 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7081 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7141 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7201 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7261 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7321 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7381 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7441 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7501 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7561 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7621 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7681 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7741 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7801 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7861 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7921 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7981 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8041 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8101 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8161 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8221 ccctttcgtc
//
