LOCUS       Exported               10588 bp ds-DNA     circular SYN 09-SEP-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Aleksandar Vjestica
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 9, 2019 from SnapGene 4.3.7
            https://www.snapgene.com

FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..567
                     /label=3''region ade6
                     /note="3''region ade6"
     misc_feature    568..836
                     /label=5'region ade6
                     /note="5'region ade6"
     CDS             837..2495
                     /label=ORF ade6
                     /note="ORF ade6"
     misc_feature    2496..2641
                     /label=3'region ade6
                     /note="3'region ade6"
     misc_feature    2697..3152
                     /label=F1 ori
                     /note="F1 ori"
     primer_bind     3292..3309
                     /label=M13 forward
                     /note="M13 forward"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7"
     promoter        3358..4004
                     /label=promoter atb2
                     /note="promoter atb2"
     CDS             4014..4727
                     /codon_start=1
                     /label=sfGFP
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             4734..6083
                     /codon_start=1
                     /label=atb2 ORF
                     /note="atb2 ORF"
                     /translation="MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNS
                     DGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYA
                     RGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTK
                     KSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERP
                     SYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKA
                     FHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRT
                     IQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMY
                     SKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSMEVDYMEEEY"
     misc_feature    6084..6643
                     /label=3'region atb2
                     /note="3'region atb2"
     misc_feature    6084..6499
                     /label=3'UTR atb2
                     /note="3'UTR atb2"
     misc_feature    6650..7032
                     /label=Ashbya gossypii TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7033..8061
                     /label=hph ORF
                     /note="hph ORF"
     misc_feature    8062..8294
                     /label=Ashbya gossypii TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(8326..8345)
                     /label=T3
                     /note="T3"
     primer_bind     complement(8363..8383)
                     /label=M13 reverse
                     /note="M13 reverse"
     protein_bind    complement(8389..8411)
                     /label=lacO
                     /note="lacO"
     promoter        complement(8415..8445)
                     /label=lac promoter
                     /note="lac promoter"
     rep_origin      complement(8751..9379)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     CDS             complement(9528..10388)
                     /label=AmpR
                     /note="AmpR"
     promoter        complement(10389..10493)
                     /label=AmpR promoter
                     /note="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccaaa
     3361 taaatcaaaa ctttttccgc gtaactctag tataagccgg aacaaggcta agaaacaaat
     3421 aaatgcaacg tttttatatt tcgatctatc aaatcctcaa ttacggaagg gcaattagta
     3481 gcgacacaag agatatacgc tgctgacaag tttttcgtcg taataaaaga gcttttgaag
     3541 agaaaaaaat tacggcggag cgttgcaatt cgttatatat aatgtgctat gcataaactc
     3601 tacttttgct aggaattctc cttgccctta tgtaatattt tatatacttt taaaaagatt
     3661 tacatgagga tatacctgtt ctataaagga gcacatgttg agttttaatg gaaatttacc
     3721 aatccaatct ttcacttaca aattgagaaa ataaaaggtg aaattgatga agatcctaat
     3781 ctttttatga ttagaaagct taaaacacct gactgagtaa gcttcttttt gcaagcaatg
     3841 cattatttta atgtctattt acatgtttag catacttcct tccgtatcat caataaatat
     3901 cgttagactt ctttattaag gaattgtttt gattaagtgt acaccgcact tcactacaaa
     3961 ccaaacacca aaaacctcgt agaaaccctg gaaagacgtt cgaattaatt aaaatgtcca
     4021 agggtgaaga gctatttact ggggttgtac ccattttggt agaactggac ggagatgtaa
     4081 acggacataa attctctgtt agaggtgagg gcgaaggcga tgccaccaat ggtaaattga
     4141 ctctgaagtt tatatgcact acgggtaaat tacctgttcc ttggccaacc ctagtaacaa
     4201 ctttgacata tggtgttcaa tgtttctcaa gatacccaga ccatatgaaa aggcatgatt
     4261 tctttaaaag tgctatgcca gaaggctacg tgcaagagag aactatctcc tttaaggatg
     4321 acggtacgta taaaacacga gcagaagtga aattcgaagg ggatacacta gttaatcgca
     4381 tcgaattaaa gggtatagac tttaaggaag atggtaatat tctcggccat aaacttgagt
     4441 ataatttcaa ctcgcataat gtgtacatta cagctgacaa acaaaagaac ggaattaaag
     4501 cgaattttaa aatcaggcac aacgtcgaag atgggtctgt tcaacttgcc gatcattatc
     4561 agcaaaacac ccctattggt gatggtccag tcttgttacc cgataatcac tacttaagca
     4621 cacagtctag attgtcaaaa gatccgaatg aaaagcgtga tcacatggtt ttattggaat
     4681 ttgtcaccgc tgcaggaata actcacggaa tggacgagct ttataaggtc gacatgagag
     4741 agatcatttc cattcacgtt ggccaagcag gaactcaaat cggaaatgct tgttgggaac
     4801 tgtattgttt agaacatggt attcaaccaa atggttacat gaatcctgag actgcatccc
     4861 aaaatagtga tggcgggttt tctacttttt tctcagaaac tggtcaagga aaatacgttc
     4921 ctagaagtat ttatgtagat cttgaaccga atgtgatcga ccaagttcgt actggcccat
     4981 accgtgatct tttccatcca gagcaattaa ttacgggcaa ggaagatgca tcaaacaatt
     5041 atgctcgtgg tcactacact gttggaaaag agctggttga tgaagtaact gacaagattc
     5101 gtcgtattgc cgataattgc tccggccttc agggctttct cgtcttccat tcgtttggag
     5161 gtggtaccgg ttcaggcttt ggtgcccttt tacttgaacg cttggccatg gaatacacga
     5221 aaaaatcaaa gcttcaattt tctgtgtatc cggctcctca agttagtact tctgtagttg
     5281 aaccttataa ctccgttttg actacccatg ctacgttgga ccttgctgac tgtacattta
     5341 tggttgacaa cgaatcgtgc tatgatattt gccgtagaaa cttagatatt gagcgtcctt
     5401 cctatgagaa tctcaataga ttgatcgctc aggttgtttc ttccatcacc gcctctcttc
     5461 gcttcgaggg cagcttgaat gttgatttgg cagaattcca aacgaatttg gtaccttatc
     5521 ctcgtattca tttccctctc gtcacttatg caccaattgt tagtgccgcc aaggccttcc
     5581 atgaatctaa cagcgttcaa gaaatcacca atcaatgttt tgaaccctac aaccaaatgg
     5641 ttaaatgtga tcctcgtgcc ggtcgctaca tggcaacgtg tttgctgtat cgtggtgatg
     5701 ttatccctcg cgatgttcaa gctgctgtaa cgaccattaa ggccaagcgt acaattcagt
     5761 tcgttgactg gtgtcctact ggattcaaaa tcggtatttg tgatcgtcca ccccaacaca
     5821 ttgaaggtag tgaaattgct aaggttgacc gtgccgtctg tatgctctcc aatactacct
     5881 ctattgcgga ggcttggtca aggctcgatc acaaatttga tctcatgtat agcaagcgtg
     5941 cttttgttca ttggtatgtt ggtgaaggta tggaagaagg cgagttttct gaagctcgtg
     6001 aggatcttgc tgctcttgaa cgcgattatg aggaagttgg tcaagattcg atggaagtcg
     6061 attacatgga agaagagtac taaagaattt cgtttatatg ctttaaagat tttcatttaa
     6121 taaccttctt ttatacgtat tatatttttt ttttactttt tctcaatctc tctcacactc
     6181 tctacatgac atatacaaaa ttgctaatac tacttttctt ttaccttaca aatgttgcaa
     6241 tcggtgaacg ctgtcaaagt ttattcacaa taactagaag tataaatttt cttttgacat
     6301 tcccatgcaa ctatgcattc cattccttta cgtgtctttc tttctggttg catcgtcatt
     6361 agattaaact tgtgctatta tggttgcaac cacttgttta ctgcactctt ttttggttct
     6421 acagctttcc ccgattagtt tacattttca atacccatat ttttttatcc tattagatat
     6481 ccgtaaactg ttttgaaact aatttctctt ccgagtcgtg ttaagaatag aaatagccgt
     6541 ttaacacttg cgtaagcata tactttctgt ttgtaaagct tggggatatt taattggaat
     6601 tttttgtata taataattgt gagattgcta aacgaaataa tgtgagctcg tttagcttgc
     6661 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
     6721 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
     6781 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
     6841 ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
     6901 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
     6961 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
     7021 acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     7081 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     7141 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     7201 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     7261 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     7321 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     7381 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     7441 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     7501 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     7561 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     7621 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     7681 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     7741 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     7801 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     7861 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     7921 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     7981 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     8041 cgtccgaggg caaaggaata atcagtactg acaataaaaa gattcttgtt ttcaagaact
     8101 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
     8161 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
     8221 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
     8281 aacgccgcca tccacctgca ggtaatcaca cgtgccagct tttgttccct ttagtgaggg
     8341 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8401 ctcacaattc cacacaacat aggagccgga agcataaagt gtaaagcctg gggtgcctaa
     8461 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8521 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8581 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8761 ctggcgtttt tccataggct cggcccccct gacgagcatc acaaaaatcg acgctcaagt
     8821 cagaggtggc gaaacccgac aggactataa agataccagg cgttcccccc tggaagctcc
     8881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8941 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     9001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9181 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactg
     9601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9901 caacgatcaa ggcgagttac atgatccccc atgttgtgaa aaaaagcggt tagctccttc
     9961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10501 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    10561 aataggcgta tcacgaggcc ctttcgtc
//
