LOCUS       pHD-Attp-3xP3DsR        4081 bp    DNA     circular SYN 07-Aug-2019
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     regulatory      860..951
                     /note="Amp Promoter"
     misc_structure  1236..1284
                     /note="MCS Site 1 (AarI flanked)"
     misc_structure  1289..1310
                     /note="Insert Digestion Sites by Q5 SDM"
     misc_recomb     <1312..1321
                     /note="Remainder of AttP Site after Mutation of
                     pHD-3XP3-DsRed"
     misc_recomb     1322..1355
                     /note="LoxP Site"
     regulatory      1362..1567
                     /note="3xP3 Enhancer + hsp70 min"
     gene            1587..2264
                     /note="DsRed"
     misc_recomb     2513..2546
                     /note="LoxP Site 2"
     misc_structure  2548..2613
                     /note="MCS 2 (SapI flanked)"
     oriT            3368..3956
BASE COUNT      1057 A      1051 C       969 G      1004 T         0 OTHER
ORIGIN      ?
        1 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
       61 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
      121 GCGCTGCGAT GATACCGCGA GAACCACGCT CACCGGCTCC GGATTTATCA GCAATAAACC
      181 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
      241 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
      301 TTGTTGCCAT CGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
      361 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
      421 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
      481 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
      541 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
      601 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
      661 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
      721 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
      781 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
      841 GGAAATGTTG AATACTCATA TTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
      901 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCA
      961 GTGTTACAAC CAATTAACCA ATTCTGAACA TTATCGCGAG CCCATTTATA CCTGAATATG
     1021 GCTCATAACA CCCCTTGTTT GCCTGGCGGC AGTAGCGCGG TGGTCCCACC TGACCCCATG
     1081 CCGAACTCAG AAGTGAAACG CCGTAGCGCC GATGGTAGTG TGGGGACTCC CCATGCGAGA
     1141 GTAGGGAACT GCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT GGGCCTTTCG
     1201 CCCGGGCTAA TTATGGGGTG TCGCCCTTCG CTGAAGCAGG TGGAATTCTT GCATGCTAGC
     1261 GGCCGCGGAC ATATGCACAC CTGCGATCGA CGCGTAGTCG ATCGCGGTAC CGGGGGCGTA
     1321 GATAACTTCG TATAATGTAT GCTATACGAA GTTATCGTAC GGGATCTAAT TCAATTAGAG
     1381 ACTAATTCAA TTAGAGCTAA TTCAATTAGG ATCCAAGCTT ATCGATTTCG AACCCTCGAC
     1441 CGCCGGAGTA TAAATAGAGG CGCTTCGTCT ACGGAGCGAC AATTCAATTC AAACAAGCAA
     1501 AGTGAACACG TCGCTAAGCG AAAGCTAAGC AAATAAACAA GCGCAGCTGA ACAAGCTAAA
     1561 CAATCGGCTC GAAGCCGGTC GCCACCATGG CCTCCTCCGA GGACGTCATC AAGGAGTTCA
     1621 TGCGCTTCAA GGTGCGCATG GAGGGCTCCG TGAACGGCCA CGAGTTCGAG ATCGAGGGCG
     1681 AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA GCTGAAGGTG ACCAAGGGCG
     1741 GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCCCAGTT CCAGTACGGC TCCAAGGTGT
     1801 ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACAAGAAGCT GTCCTTCCCC GAGGGCTTCA
     1861 AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT GACCGTGACC CAGGACTCCT
     1921 CCCTCCAGGA CGGCTCCTTC ATCTACAAGG TGAAGTTCAT CGGCGTGAAC TTCCCCTCCG
     1981 ACGGCCCCGT AATGCAGAAG AAGACTATGG GCTGGGAGGC GTCCACCGAG CGCCTGTACC
     2041 CCCGCGACGG CGTGCTGAAG GGCGAGATCC ACAAGGCCCT GAAGCTGAAG GACGGCGGCC
     2101 ACTACCTGGT GGAGTTCAAG TCCATCTACA TGGCCAAGAA GCCCGTGCAG CTGCCCGGCT
     2161 ACTACTACGT GGACTCCAAG CTGGACATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG
     2221 AGCAGTACGA GCGCGCCGAG GGCCGCCACC ACCTGTTCCT GTAGGGGCCG CGACTCTAGA
     2281 TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC
     2341 TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG
     2401 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
     2461 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACCG GTATAACTTC
     2521 GTATAATGTA TGCTATACGA AGTTATAGAA GAGCACTAGT AAAGATCTCC ATGCATAAGG
     2581 CGCGCCTAGG CCTTCTGCAG CTCGAGGCTC TTCCGTCAAT CGAGTTCAAG GGCGACACAA
     2641 AATTTATTCT AAATGCATAA TAAATACTGA TAACATCTTA TAGTTTGTAT TATATTTTGT
     2701 ATTATCGTTG ACATGTATAA TTTTGATATC AAAAACTGAT TTTCCCTTTA TTATTTTCGA
     2761 GATTTATTTT CTTAATTCTC TTTAACAAAC TAGAAATATT GTATATACAA AAAATCATAA
     2821 ATAATAGATG AATAGTTTAA TTATAGGTGT TCATCAATCG AAAAAGCAAC GTATCTTATT
     2881 TAAAGTGCGT TGCTTTTTTC TCATTTATAA GGTTAAATAA TTCTCATATA TCAAGCAAAG
     2941 TGACAGGCGC CCTTAAATAT TCTGACAAAT GCTCTTTCCC TAAACTCCCC CCATAAAAAA
     3001 ACCCGCCGAA GCGGGTTTTT ACGTTATTTG CGGATTAACG ATTACTCGTT ATCAGAACCG
     3061 CCCAGGGGGC CCGAGCTTAA GACTGGCCGT CGTTTTACAA CACAGAAAGA GTTTGTAGAA
     3121 ACGCAAAAAG GCCATCCGTC AGGGGCCTTC TGCTTAGTTT GATGCCTGGC AGTTCCCTAC
     3181 TCTCGCCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
     3241 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
     3301 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
     3361 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
     3421 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     3481 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     3541 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     3601 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     3661 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     3721 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
     3781 GGTGGGCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
     3841 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     3901 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     3961 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGACGCGCGC GTAACTCACG
     4021 TTAAGGGATT TTGGTCATGA GCTTGCGCCG TCCCGTCAAG TCAGCGTAAT GCTCTGCTTT
     4081 T
//
