LOCUS       ___10_PB_CAG_L_3        8615 bp ds-DNA     circular     20-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
COMMENT     LOCUS GS56925 pBSK-seq56925 3680 bp DNA circular SYN 19-DEC-2014
            DEFINITION Ligation of inverted seq56925 into modified pBSK
            ACCESSION GS56925 pBSK-seq56925 KEYWORDS . SOURCE Unknown. ORGANISM
            Unknown Unclassified. REFERENCE 1 (bases 1 to 3680) AUTHORS Self
            JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager,
            Scientific & Educational Software COMMENT SECNOTES|Vector molecule:
            modified pBSK Fragment ends: SmaI Fragment size: 2848 Insert
            molecule: seq56925 Fragment ends: blunt Fragment size: 830
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             129..986
                     /locus_tag="Amp+"
                     /label="Amp+"
                     /ApEinfo_label="Amp+"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1137..1804)
                     /locus_tag="pUC origin"
                     /label="pUC origin"
                     /ApEinfo_label="pUC origin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2200..2606)
                     /note="3' piggyBac TR"
                     /locus_tag="3' piggyBac TR"
                     /label="3' piggyBac TR"
                     /ApEinfo_label="3' piggyBac TR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     enhancer        2620..2997
                     /note="hCMV enhancer"
                     /locus_tag="hCMV enhancer"
                     /label="hCMV enhancer"
                     /ApEinfo_label="hCMV enhancer"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(2943..2964)
                     /note="HN853"
                     /locus_tag="HN853"
                     /label="HN853"
                     /ApEinfo_label="HN853"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        3003..4344
                     /note="AG hybrid promoter"
                     /locus_tag="AG hybrid promoter"
                     /label="AG hybrid promoter"
                     /ApEinfo_label="AG hybrid promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3003..3280
                     /note="Chicken b-actin promoter"
                     /locus_tag="Chicken b-actin promoter"
                     /label="Chicken b-actin promoter"
                     /ApEinfo_label="Chicken b-actin promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3190..3194
                     /note="CCATT box"
                     /locus_tag="CCATT box"
                     /label="CCATT box"
                     /ApEinfo_label="CCATT box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3252..3259
                     /note="TATA box"
                     /locus_tag="TATA box"
                     /label="TATA box"
                     /ApEinfo_label="TATA box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3264..3280
                     /note="TSS"
                     /locus_tag="TSS"
                     /label="TSS"
                     /ApEinfo_label="TSS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3281..3371
                     /note="Chicken b-actin exon 1"
                     /locus_tag="Chicken b-actin exon 1"
                     /label="Chicken b-actin exon 1"
                     /ApEinfo_label="Chicken b-actin exon 1"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3372..4296
                     /note="Chicken b-actin rabbit globin hybrid intron"
                     /locus_tag="Chicken b-actin rabbit globin hybrid"
                     /label="Chicken b-actin rabbit globin hybrid"
                     /ApEinfo_label="Chicken b-actin rabbit globin hybrid"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3372..4247
                     /note="Chicken b-actin intron"
                     /locus_tag="Chicken b-actin intron"
                     /label="Chicken b-actin intron"
                     /ApEinfo_label="Chicken b-actin intron"
                     /ApEinfo_fwdcolor="#eb606c"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4248..4344
                     /note="Rabbit b-globin splice acceptor"
                     /locus_tag="Rabbit b-globin splice acceptor"
                     /label="Rabbit b-globin splice acceptor"
                     /ApEinfo_label="Rabbit b-globin splice acceptor"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4261..4281
                     /note="HN737"
                     /locus_tag="HN737"
                     /label="HN737"
                     /ApEinfo_label="HN737"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4305..4324
                     /note="HN382"
                     /locus_tag="HN382"
                     /label="HN382"
                     /ApEinfo_label="HN382"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(4356..4389)
                     /note="loxP"
                     /locus_tag="loxP"
                     /label="loxP"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4416..4643
                     /note="SV40 pA"
                     /locus_tag="SV40 pA"
                     /label="SV40 pA"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4649..4876
                     /note="SV40 pA"
                     /locus_tag="SV40 pA(1)"
                     /label="SV40 pA(1)"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4882..5109
                     /note="SV40 pA"
                     /locus_tag="SV40 pA(2)"
                     /label="SV40 pA(2)"
                     /ApEinfo_label="SV40 pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            complement(5132..5165)
                     /note="loxP"
                     /locus_tag="loxP(1)"
                     /label="loxP(1)"
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5175..6635
                     /note="tdGFP"
                     /locus_tag="tdGFP"
                     /label="tdGFP"
                     /ApEinfo_label="tdGFP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5175..5888
                     /note="superfolder GFP"
                     /locus_tag="superfolder GFP"
                     /label="superfolder GFP"
                     /ApEinfo_label="superfolder GFP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5889..5918
                     /note="linker"
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6644..7229
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7250..7551
                     /note="bGH pA"
                     /locus_tag="bGH pA"
                     /label="bGH pA"
                     /ApEinfo_label="bGH pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7558..7964
                     /note="5' piggyBac TR"
                     /locus_tag="5' piggyBac TR"
                     /label="5' piggyBac TR"
                     /ApEinfo_label="5' piggyBac TR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             8001..8156
                     /locus_tag="lacZ"
                     /label="lacZ"
                     /ApEinfo_label="lacZ"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(8289..8595)
                     /locus_tag="f1 origin"
                     /label="f1 origin"
                     /ApEinfo_label="f1 origin"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacctccccc ggtattcacg acagcaggct
     2221 gaataataaa aaaattagaa actattattt aaccctagaa agataatcat attgtgacgt
     2281 acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
     2341 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
     2401 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa
     2461 aatttcttct ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta
     2521 ttttgtactt taaaaacagt catgttgtat tataaaataa gtaattagct taacctatac
     2581 ataatagaaa caaattatac ttattaaccg gtcatggtcg acattgatta ttgactagtt
     2641 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2701 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2761 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2821 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2881 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2941 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3001 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     3061 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
     3121 ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg
     3181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
     3241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc
     3301 cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     3361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     3421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     3481 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     3541 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     3601 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     3661 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     3721 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     3781 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3901 gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     3961 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     4021 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     4081 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     4141 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     4201 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     4261 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     4321 gtgctgtctc atcattttgg caaagaattg ctagcataac ttcgtatagc atacattata
     4381 cgaagttata gccaccgcgg tggcggccgc tctagaatca taatcagcca taccacattt
     4441 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     4501 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     4561 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4621 tccaaactca tcaatgtatc ttaactagaa tcataatcag ccataccaca tttgtagagg
     4681 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4741 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4861 tcatcaatgt atcttaacta gaatcataat cagccatacc acatttgtag aggttttact
     4921 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4981 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5041 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5101 tgtatcttaa ctagtggatc tcatgcctag gataacttcg tatagcatac attatacgaa
     5161 gttataggcg cgccatgagc aaaggagaag aacttttcac tggagttgtc ccaattcttg
     5221 ttgaattaga tggtgatgtt aatgggcaca aattttctgt ccgtggagag ggtgaaggtg
     5281 atgctacaaa cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc
     5341 cgtggccaac acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg
     5401 atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
     5461 gcactatatc tttcaaagat gacgggacct acaagacgcg tgctgaagtc aagtttgaag
     5521 gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca
     5581 ttcttggaca caaactcgaa tacaacttta actcacacaa tgtatacatc acggcagaca
     5641 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacgttgaa gatggttccg
     5701 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     5761 cagacaacca ttacctgtcg acacaatctg tcctttcgaa agatcccaac gaaaagcgtg
     5821 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
     5881 tctacaaagg ttctgctgga tctgcagcag gatctggtat gagcaaagga gaagaacttt
     5941 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     6001 ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa actcaccctt aaatttattt
     6061 gcactactgg aaaactacct gttccgtggc caacacttgt cactactctg acctatggtg
     6121 ttcaatgctt ttcccgttat ccggatcaca tgaaacggca tgactttttc aagagtgcca
     6181 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg acctacaaga
     6241 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaagggta
     6301 ttgattttaa agaagatgga aacattcttg gacacaaact cgaatacaac tttaactcac
     6361 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
     6421 gccacaacgt tgaagatggt tccgttcaac tagcagacca ttatcaacaa aatactccaa
     6481 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgtccttt
     6541 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
     6601 ggattacaca tggcatggat gagctctaca aatgattaat taaaacctct ggattacaaa
     6661 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6721 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6781 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6841 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6901 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6961 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7021 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7081 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7141 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7201 cggatctccc tttgggccgc ctccccgcag gatccactag agcggccgcc tagagctcgc
     7261 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7321 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7381 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7441 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     7501 tctgaggcgg aaagaaccag ctggggctcg actagagctt gcggaaccct taagcttttt
     7561 tattatatat tagtcacgat atctataaca agaaaatata tatataataa gttatcacgt
     7621 aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa
     7681 aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt
     7741 accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc
     7801 acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca
     7861 attttacgca gactatcttt ctagggttaa aaaagatttg cgctttactc gacctaaact
     7921 ttaaacacgt catagaatct tcgtttgaca aaaaccacat tgtgttgggt cgccctatag
     7981 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8041 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8101 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat
     8161 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     8221 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     8281 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     8341 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     8401 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     8461 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     8521 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     8581 cgccgcgctt aatgcgccgc tacagggcgc gtcag  
//
