LOCUS       pUC19_TTE1564_UU        3710 bp ds-DNA     circular     15-SEP-2019
DEFINITION  Cloning vector pUC19c, complete sequence.
ACCESSION   L09137 X02514
VERSION     L09137.2  GI:20141090
KEYWORDS    .
SOURCE      Cloning vector pUC19c
  ORGANISM  Cloning vector pUC19c other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Yanisch-Perron,C., Vieira,J. and Messing,J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
   PUBMED   2985470
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
  TITLE     The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
            to facilitate the use of sonicated DNA for nucleotide sequencing
  JOURNAL   Gene 68 (1), 139-149 (1988)
   PUBMED   2851488
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Department of Biochemistry, University of
            Minnesota, St. Paul, MN 55108, USA
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2002) Rutgers, The State University of New
            Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
            Piscataway, NJ 08854-8020, USA
  REMARK    Sequence update by submitter
COMMENT     pUC19_COG1564_C15U_M1mut from 1 to 3716
COMMENT     pUC19_COG1564 from 1 to 3716
COMMENT     Alignment and annotation to NCBI reference for Tte MB4 genome -
            http://tinyurl.com/bn2w7cq  On Apr 11, 2002 this sequence version
            replaced gi:209213. These data and their annotation were supplied
            to GenBank by Will Gilbert under the auspices of the GenBank
            Currator Program. pUC19c - Cloning vector (beta-galactosidase mRNA
            on complementary strand) ENTRY PUC19C                    #TYPE DNA
            CIRCULAR TITLE pUC19c - Cloning vector (beta-galactosidase mRNA on
            complementary strand) DATE 03-FEB-1986    #sequence  16-DEC-1986
            ACCESSION VB0033 SOURCE artificial COLLECTION  ATCC 37254 REFERENCE
            #number    #authors Norrander J., Kempe T., Messing J.    #journal
            Gene (1983) 26: 101-106 REFERENCE    #number  1    #authors
            Yanisch-Perron C., Vieira J., Messing J.    #journal Gene (1985)
            33: 103-119 #comment shows the complete compiled sequence REFERENCE
            #number 2    #authors  Chambers,S.P., et al.    #journal Gene
            (1988) 68: 139-149    #describes mutation at nt1308 and its effect
            on copy number REFERENCE    #number    #authors Pouwels P.H.,
            Enger-Valk B.E., Brammar W.J.    #book    Cloning Vectors, Elsvier
            1985 and supplements    #comment  vector I-A-iv-20 COMMENT    This
            Sequence was obtained 3-MAR-1986 from J. Messing, Waksman
            Institute, NJ on floppy disk.    Revised 16-DEC-1986 by F.
            Pfeiffer:    1062/3 'AT' to 'TA' to match revised sequence of
            PBR322    The beta-galactosidase mRNA sequence including the
            multiple    cloning site of M13mp19 is on the strand complementary
            to that    shown. KEYWORDS CROSSREFERENCE    #complement
            VecBase(3):pUC19 #prerevised      GenBank(50):M11662,
            EMBL(11):ARPuc19    #parent VecBase(3):pUC13, VecBase(3):M13mp19,
            VecSource(3):bGal19 PARENT Features of pUC19c (2686 bp) residue
            source       1- 137 2074-2210 pBR322     138- 237 2252-2351 pBR322
            238- 395 1461-1304 (c) Lac-Operon     396- 452 57-   1 (c)
            polylinker of M13mp19     455- 682  1298-1071 (c) Lac-Operon
            683-2686 2352-4355 pBR322    Conflict (cfl) and Mutations (mut):
            pUC19c  source    mut 1308  A  G  2977   pBR322 linked to increased
            copy number    mut 1942  A  G  3611   pBR322 mut 2243 T  C  3912
            pBR322 FEATURE    1629-2417 789-1 (c) Ap-R; b-lactamase POLYLINKER
            HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI SELECTION
            #resistance  Ap    #indicator beta-galactosidase SUMMARY  pUC19c
            #length 2686   #checksum 4465.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     423..442
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1488..1505)
                     /locus_tag="M13-rev (DNA Core)"
                     /label="M13-rev (DNA Core)"
                     /ApEinfo_label="M13-rev (DNA Core)"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1873..2555)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label="LacZ alpha"
                     /ApEinfo_label="LacZ alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(1511..1533)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(2653..3312)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(29..51)
                     /locus_tag="pGEX 3 primer"
                     /label="pGEX 3 primer"
                     /ApEinfo_label="pGEX 3 primer"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          join(442..460,504..1471)
                     /locus_tag="NC_003869.1 -1523276..-1522241"
                     /label="NC_003869.1 -1523276..-1522241"
                     /ApEinfo_label="NC_003869.1 -1523276..-1522241"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            962..1380
                     /locus_tag="GeneID 995665"
                     /label="GeneID 995665"
                     /ApEinfo_label="GeneID 995665"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             976..1380
                     /locus_tag="COG1563 7-cyano-7-deazaguanine reductase"
                     /label="COG1563 7-cyano-7-deazaguanine reductase"
                     /ApEinfo_label="COG1563 7-cyano-7-deazaguanine reductase"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            504..986
                     /locus_tag="GeneID 995666"
                     /label="GeneID 995666"
                     /ApEinfo_label="GeneID 995666"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             504..986
                     /note="Best Blastp hit =
                     gi|12723715|gb|AAK04893.1|AE006312_13 (AE006312)
                     HYPOTHETICAL PROTEIN [Lactococcus lactis subsp. lactis],
                     score 71.6, E-value 6.00E-12"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_623164.1"
                     /db_xref="GI:20807993"
                     /db_xref="GeneID:995666"
                     /locus_tag="COG1564 Hypothetical protein"
                     /label="COG1564 Hypothetical protein"
                     /ApEinfo_label="COG1564 Hypothetical protein"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          461..469
                     /locus_tag="NC_003869.1 -1523276..-1522241(1)"
                     /label="NC_003869.1 -1523276..-1522241(1)"
                     /ApEinfo_label="NC_003869.1 -1523276..-1522241"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          474..503
                     /locus_tag="NC_003869.1 -1523276..-1522241(2)"
                     /label="NC_003869.1 -1523276..-1522241(2)"
                     /ApEinfo_label="NC_003869.1 -1523276..-1522241"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="#ffffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            491..503
                     /locus_tag="GeneID 995666(1)"
                     /label="GeneID 995666(1)"
                     /ApEinfo_label="GeneID 995666"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1519..1536)
                     /locus_tag="LacO_01F"
                     /label="LacO_01F"
                     /ApEinfo_label="LacO_01F"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     378..395
                     /locus_tag="M13FORW (DNA Core)"
                     /label="M13FORW (DNA Core)"
                     /ApEinfo_label="M13FORW (DNA Core)"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     variation       470..473
                     /locus_tag="UUCG tetraloop"
                     /label="UUCG tetraloop"
                     /ApEinfo_label="UUCG tetraloop"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     361..378
                     /locus_tag="pUC19_Univ_01F"
                     /label="pUC19_Univ_01F"
                     /ApEinfo_label="pUC19_Univ_01F"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctaatacga ctcactatag ggcagtgagc aacaaaatgc tcacctgggT TCGcccagtt
      481 aacaaaacaa gggaggtaat tttGTGCCCA AAAAAAGAAT AAAAGATTTA GCTGAAATTG
      541 CTCTTGTTGC AGCAATTTAT TTCGCACTCA CAATTATATT TTCGTCCATT TCGTTTTTAC
      601 CCGTTCAATT TCGAATCGGG GAAATTACGA AATCCATTGT AGTATTCAAT AAAAAATATG
      661 CTATTTCCAT GATGATAGGA AATTTTTTTG CAAATTTGTT TAGCCCATTT GCTGGTGCAA
      721 TGGAATTAAT TTTTATGCCT CTTTCGAACT TAATAGGCTG TACAATTGGA TACTACATTG
      781 GAAGACTTAC TCACAAAGCG ATAGGAGCTA TATTCATAGC CCTTTGGATT GCAGCATCAG
      841 TTGCAATTAC TTTAAAGGTT TCTGCAGGCA TACCATTTAT TCCGACTTTC TTAAGCGTGG
      901 GAGTAGCGGA AACTGTACTT TTGGTAACTG GATATTTTTT GCTTTTCACA ATTGAAAAGA
      961 AAGGAGTTGT GAAATTTGAC AGATAAATAT AAAGAGAGAA GATTTGACAT TTACGGTTAC
     1021 GAAAAAATTG ACAAAGAAGT TCTAGAATCT ATTGAATATG AGTATCCTGA AAAAAATACT
     1081 ATCGTGGAGT ATATTACCGA TGAATTTTCT TCTGTTTGCC CTTGGACAGG ATTACCTGAC
     1141 AATGCAAAAC TTACTATAAG GTATATACCC CACAAAAAAC TTGTAGAACT TAAATCCTTA
     1201 AAATATTACC TTACATCTTA TAGGAATGTA GGTATATTGC AAGAACATGC AATAAACAGA
     1261 ATTTTAGATG ATTTGGTGGA ATTCCTGCAG CCAAAATTTA TGGAAATAAT AGGCGAATTT
     1321 CAGGAAAGAG GAGGAATAGC TACAAGAATT ATAGCAAGGT ATGAAAAAGA GGAGTATTAA
     1381 acttaaaagg ctgcctaaaa ttttgtaggc agctttttta ttcattttag tttttcttca
     1441 aaatgagttt acagagaacg atgaggaagc aagcttggcg taatcatggt catagctgtt
     1501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     3661 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
//
