LOCUS       GWF180                 15275 bp ds-DNA     circular     15-APR-2019
DEFINITION  DNCR2-VPR-IRES-Puro-WPRE-SV40PA-NS3aH1-BFP-SpdCas9-2xNLS.
ACCESSION   urn.local...p-aykyd2m
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    1..1351
                     /ApEinfo_fwdcolor="#ffff66"
                     /ApEinfo_revcolor="#ffff66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CAG promoter"
     misc_feature    592..600
                     /Recognition_pattern="GCGC^NGCGC"
                     /annotation_group="GCGCNGCGC"
                     /label="MteI"
                     /note="Geneious type: restriction site"
     misc_feature    940..947
                     /Recognition_pattern="CR^CCGGYG"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    940..947
                     /Recognition_pattern="CRCCGGYG"
                     /annotation_group="CRCCGGYG"
                     /label="PsuNI, SvoI"
                     /note="Geneious type: restriction site"
     misc_feature    940..947
                     /Recognition_pattern="CG^CCGGCG"
                     /annotation_group="CGCCGGCG"
                     /label="MreI, Sse232I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1007..1021)
                     /Recognition_pattern="CRAANNNNNNNNCTG"
                     /annotation_group="CRAANNNNNNNNCTG"
                     /label="SpnD39IIIA"
                     /note="Geneious type: restriction site"
     misc_feature    1323..1336
                     /Recognition_pattern="TTANNNNNNNGTCY"
                     /annotation_group="TTANNNNNNNGTCY"
                     /label="EcoDI"
                     /note="Geneious type: restriction site"
     gene            1364..2053
                     /created_by="User"
                     /modified_by="User"
                     /label="DNCR2"
     misc_feature    1364..1366
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Start codon"
     misc_feature    1367..2083
                     /Original_Bases="GGTGGGGGCGCGCCAACTCAAGACGAATTCACCCAACTCTC
                     CCAATCAATCGCCGAGTTCCACACGTACCAACTCGGTAACGGCCGTTGCTCATCTCTC
                     CTAGCTCAGCGAATCCACGCGCCGCCGGAAACAGTATGGTCCGTGGTGAGACGTTTCG
                     ATAGGCCACAGATTTACAAACACTTCATCAAAAGCTGTAACGTGAGTGAAGATTTCGA
                     GATGCGAGTGGGATGCACGCGCGACGTGAACGTGATAAGTGGATTACCGGCGAATACG
                     TCTCGAGAGAGATTAGATCTGTTGGACGATGATCGGAGAGTGACTGGGTTTAGTATAA
                     CCGGTGGTGAACATAGGCTGAGGAATTATAAATCGGTTACGACGGTTCATAGATTTGA
                     GAAAGAAGAAGAAGAAGAAAGGATCTGGACCGTTGTTTTGGAATCTTATGTTGTTGAT
                     GTACCGGAAGGTAATTCGGAGGAAGATACGAGATTGTTTGCTGATACGGTTATTAGAT
                     TGAATCTTCAGAAACTTGCTTCGATCACTGAAGCTATGAAC"
                     /label="GGTGGGGGCGCGCCAACTCAAGACGAATTCACCCAACTCTCCCAATCAAT
                     CGCCGAGTTCCACACGTACCAACTCGGTAACGGCCGTTGCTCATCTCTCCTAGCTCAG
                     CGAATCCACGCGCCGCCGGAAACAGTATGGTCCGTGGTGAGACGTTTCGATAGGCCAC
                     AGATTTACAAACACTTCATCAAAAGCTGTAACGTGAGTGAAGATTTCGAGATGCGAGT
                     GGGATGCACGCGCGACGTGAACGTGATAAGTGGATTACCGGCGAATACGTCTCGAGAG
                     AGATTAGATCTGTTGGACGATGATCGGAGAGTGACTGGGTTTAGTATAACCGGTGGTG
                     AACATAGGCTGAGGAATTATAAATCGGTTACGACGGTTCATAGATTTGAGAAAGAAGA
                     AGAAGAAGAAAGGATCTGGACCGTTGTTTTGGAATCTTATGTTGTTGATGTACCGGAA
                     GGTAATTCGGAGGAAGATACGAGATTGTTTGCTGATACGGTTATTAGATTGAATCTTC
                     AGAAACTTGCTTCGATCACTGAAGCTATGAAC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1705..1726
                     /created_by="User"
                     /label="HSinsert_fwd"
     misc_feature    complement(1934..1953)
                     /created_by="User"
                     /label="HSinsert_rev"
     misc_feature    2054..2083
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Myc Tag"
     misc_feature    2105..2110
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="AclNI, AhlI, BcuI, SpeI"
                     /note="Geneious type: restriction site"
     misc_feature    2111..2223
                     /ApEinfo_fwdcolor="#83ff61"
                     /ApEinfo_revcolor="#83ff61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="VP64 AD domain"
     misc_feature    2225..2266
                     /ApEinfo_label="VP64 AD domain"
                     /ApEinfo_fwdcolor="#83ff61"
                     /ApEinfo_revcolor="#83ff61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="VP64 AD domain(1)"
     misc_feature    2267..2287
                     /ApEinfo_fwdcolor="#ff986a"
                     /ApEinfo_revcolor="#ff986a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NLS signal"
     misc_feature    2288..2300
                     /Recognition_pattern="GGANNNNNNTATC"
                     /annotation_group="GGANNNNNNTATC"
                     /label="Eco1348I, Eco1412I, Eco777I"
                     /note="Geneious type: restriction site"
     misc_feature    2294..3088
                     /ApEinfo_fwdcolor="#55ffa9"
                     /ApEinfo_revcolor="#55ffa9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="p65 AD domain"
     misc_feature    2370..2383
                     /Recognition_pattern="TCANNNNNNNATCG"
                     /annotation_group="TCANNNNNNNATCG"
                     /label="EcoDR3"
                     /note="Geneious type: restriction site"
     misc_feature    2599..2610
                     /Recognition_pattern="(10/12)GCANNNNNNTGC(12/10)"
                     /annotation_group="GCANNNNNNTGC"
                     /label="AlfI"
                     /note="Geneious type: restriction site"
     misc_feature    2871..2881
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    2876..2888
                     /Recognition_pattern="GGTANNNNNNTCG"
                     /annotation_group="GGTANNNNNNTCG"
                     /label="StySBLI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2876..2888)
                     /Recognition_pattern="CGANNNNNNTACC"
                     /annotation_group="CGANNNNNNTACC"
                     /label="StySENI"
                     /note="Geneious type: restriction site"
     misc_feature    3089..3106
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="linker"
     misc_feature    3107..3676
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="RTA"
     misc_feature    3323..3329
                     /Recognition_pattern="CCTCAGC(-5/-2)"
                     /annotation_group="CCTCAGC"
                     /label="AbeI, BbvCI"
                     /note="Geneious type: restriction site"
     misc_feature    3323..3329
                     /Recognition_pattern="CCTCAGC"
                     /annotation_group="CCTCAGC"
                     /label="PmdI"
                     /note="Geneious type: restriction site"
     misc_feature    3680..3687
                     /Recognition_pattern="ATTT^AAAT"
                     /annotation_group="ATTTAAAT"
                     /label="BstRZ246I, BstSWI, MspSWI, SmiI, SwaI"
                     /note="Geneious type: restriction site"
     misc_feature    3688..4279
                     /SerialCloner_Color="&hC00E0E"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /ApEinfo_fwdcolor="#3456cb"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="IRES"
     misc_feature    3845..3850
                     /Recognition_pattern="C^CTAGG"
                     /annotation_group="CCTAGG"
                     /label="AspA2I, AvrBII, AvrII, BlnI, BspA2I, XmaJI"
                     /note="Geneious type: restriction site"
     misc_feature    4135..4140
                     /Recognition_pattern="GGTACC"
                     /annotation_group="GGTACC"
                     /label="BspJ106I, Eco149I, Esp19I, KpnK14I, MvsAI, MvsBI,
                     MvsCI, MvsDI, MvsEI, MvsI, NmiI, Sau10I, SthAI, SthBI,
                     SthCI, SthDI, SthEI, SthFI, SthGI, SthHI, SthJI, SthKI,
                     SthLI, SthMI, SthNI, Uba1201I, Uba76I, Uba85I, Uba86I,
                     Uba87I"
                     /note="Geneious type: restriction site"
     misc_feature    4135..4140
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    4135..4140
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, AhaB8I, Asp718I, SthI"
                     /note="Geneious type: restriction site"
     misc_feature    4298..4900
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Puro"
     misc_feature    4340..4348
                     /Recognition_pattern="GACNNNGTC"
                     /annotation_group="GACNNNGTC"
                     /label="FsuI, NtaI, SmaAII, SpaPI, SplAII, SplII, TrsKTII,
                     TspI, TteI, TtrI"
                     /note="Geneious type: restriction site"
     misc_feature    4340..4348
                     /Recognition_pattern="GACN^NNGTC"
                     /annotation_group="GACNNNGTC"
                     /label="AspI, AtsI, BgiI, PflFI, PsyI, TelI, Tth111I"
                     /note="Geneious type: restriction site"
     misc_feature    4416..4422
                     /Recognition_pattern="CG^GWCCG"
                     /annotation_group="CGGWCCG"
                     /label="CpoI, CspI, Rsr2I, RsrII"
                     /note="Geneious type: restriction site"
     misc_feature    4435..4441
                     /Recognition_pattern="GGTNACC"
                     /annotation_group="GGTNACC"
                     /label="Bse59I, Bsp1591I, Bst31I, BstDI, Cfr19I, Cfr7I,
                     KoxI, SciAI, Uba1291I"
                     /note="Geneious type: restriction site"
     misc_feature    4435..4441
                     /Recognition_pattern="G^GTNACC"
                     /annotation_group="GGTNACC"
                     /label="AcrII, AspAI, Bse64I, BseT10I, BseT9I, BsiKI,
                     BstEII, BstPI, BstT10I, BstT9I, EcaI, Eci125I, Eco91I,
                     EcoO128I, EcoO65I, NspSAII, PspEI"
                     /note="Geneious type: restriction site"
     misc_feature    4868..4874
                     /Recognition_pattern="A^CCWGGT"
                     /annotation_group="ACCWGGT"
                     /label="CsiI, MabI, SexAI"
                     /note="Geneious type: restriction site"
     misc_feature    4901..4908
                     /Recognition_pattern="GTTT^AAAC"
                     /annotation_group="GTTTAAAC"
                     /label="MssI, PmeI"
                     /note="Geneious type: restriction site"
     enhancer        4937..5528
                     /vntifkey=9
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE"
     misc_feature    5230..5242
                     /Recognition_pattern="CCCNNNNNRTTGY"
                     /annotation_group="CCCNNNNNRTTGY"
                     /label="PacII"
                     /note="Geneious type: restriction site"
     misc_feature    5531..5661
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SV40_PA_terminator"
     misc_feature    5662..5669
                     /Recognition_pattern="CCTGCA^GG"
                     /annotation_group="CCTGCAGG"
                     /label="SbfI, SdaI, Sse8387I"
                     /note="Geneious type: restriction site"
     misc_feature    5671..6196
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="PGK Promoter"
     misc_feature    5930
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SpeI mutation"
     misc_feature    6197..6202
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="BspMKI, HgiCIII, HgiDII, NopI, RflFI, Rrh4273I,
                     Rtr63I, RtrI, SalI, XciI"
                     /note="Geneious type: restriction site"
     misc_feature    6197..6202
                     /Recognition_pattern="GTCGAC"
                     /annotation_group="GTCGAC"
                     /label="Acs1371I, Acs1372I, Acs1373I, Acs1421I, Acs1422I,
                     BbrUII, BstZ16I, BtgAI, CglAII, KoyI, Psp32I, Psp33I,
                     Psp89I, RheI, RhpI, RrhI, RroI, Uba89I, XamI"
                     /note="Geneious type: restriction site"
     misc_feature    6197..6202
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HinJCI, HincII, HindII"
                     /note="Geneious type: restriction site"
     misc_feature    6197..6202
                     /Recognition_pattern="GTYRAC"
                     /annotation_group="GTYRAC"
                     /label="ChuEII, Hin1160II, Hin1161II, MnnI"
                     /note="Geneious type: restriction site"
     misc_feature    <6212..6799
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NS3_opt_CatAct_tri_chimera GBlock"
     misc_feature    6213..6799
                     /Original_Bases="CGCGTGTGCCTTTGTATGGTTTTACTTCGATTTGTGGAAGA
                     AGACCTGAGATGGAAGCTGCTGTTTCGACTATACCAAGATTCCTTCAATCTTCCTCTG
                     GTTCGATGTTAGATGGTCGGTTTGATCCTCAATCCGCCGCTCATTTCTTCGGTGTTTA
                     CGACGGCCATGGCGGTTCTCAGGTAGCGAACTATTGTAGAGAGAGGATGCATTTGGCT
                     TTGGCGGAGGAGATAGCTAAGGAGAAACCGATGCTCTGCGATGGTGATACGTGGCTGG
                     AGAAGTGGAAGAAAGCTCTTTTCAACTCGTTCCTGAGAGTTGACTCGGAGATTGAGTC
                     AGTTGCGCCGGAGACGGTTGGGTCAACGTCGGTGGTTGCCGTTGTTTTCCCGTCTCAC
                     ATCTTCGTCGCTAACTGCGGTGACTCTAGAGCCGTTCTTTGCCGCGGCAAAACTGCAC
                     TTCCATTATCCGTTGACCATAAACCGGATAGAGAAGATGAAGCTGCGAGGATTGAAGC
                     CGCAGGAGGGAAAGTGATTCAGTGGAATGGAGCTCGTGTTTTCGGTGTTCTCGCCATG
                     TCGAGATCCATTGGCGATAGATACTTGAAACCATCCATCATTCCTGATCCGGAAGTGA
                     CGGCTGTGAAGAGAGTAAAAGAAGATGATTGTCTGATTTTGGCGAGTGACGGGGTTTG
                     GGATGTAATGACGGATGAAGAAGCGTGTGAGATGGCAAGGAAGCGGATTCTCTTGTGG
                     CACAAGAAAAACGCGGTGGCTGGGGATGCATCGTTGCTCGCGGATGAGCGGAGAAAGG
                     AAGGGAAAGATCCTGCGGCGATGTCCGCGGCTGAGTATTTGTCAAAGCTGGCGATACA
                     GAGAGGAAGCAAAGACAACATAAGTGTGGTGGTGGTTGATTTGAAG"
                     /label="CGCGTGTGCCTTTGTATGGTTTTACTTCGATTTGTGGAAGAAGACCTGAG
                     ATGGAAGCTGCTGTTTCGACTATACCAAGATTCCTTCAATCTTCCTCTGGTTCGATGT
                     TAGATGGTCGGTTTGATCCTCAATCCGCCGCTCATTTCTTCGGTGTTTACGACGGCCA
                     TGGCGGTTCTCAGGTAGCGAACTATTGTAGAGAGAGGATGCATTTGGCTTTGGCGGAG
                     GAGATAGCTAAGGAGAAACCGATGCTCTGCGATGGTGATACGTGGCTGGAGAAGTGGA
                     AGAAAGCTCTTTTCAACTCGTTCCTGAGAGTTGACTCGGAGATTGAGTCAGTTGCGCC
                     GGAGACGGTTGGGTCAACGTCGGTGGTTGCCGTTGTTTTCCCGTCTCACATCTTCGTC
                     GCTAACTGCGGTGACTCTAGAGCCGTTCTTTGCCGCGGCAAAACTGCACTTCCATTAT
                     CCGTTGACCATAAACCGGATAGAGAAGATGAAGCTGCGAGGATTGAAGCCGCAGGAGG
                     GAAAGTGATTCAGTGGAATGGAGCTCGTGTTTTCGGTGTTCTCGCCATGTCGAGATCC
                     ATTGGCGATAGATACTTGAAACCATCCATCATTCCTGATCCGGAAGTGACGGCTGTGA
                     AGAGAGTAAAAGAAGATGATTGTCTGATTTTGGCGAGTGACGGGGTTTGGGATGTAAT
                     GACGGATGAAGAAGCGTGTGAGATGGCAAGGAAGCGGATTCTCTTGTGGCACAAGAAA
                     AACGCGGTGGCTGGGGATGCATCGTTGCTCGCGGATGAGCGGAGAAAGGAAGGGAAAG
                     ATCCTGCGGCGATGTCCGCGGCTGAGTATTTGTCAAAGCTGGCGATACAGAGAGGAAG
                     CAAAGACAACATAAGTGTGGTGGTGGTTGATTTGAAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6290..6307
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Hydrophobic Helix"
     misc_feature    6441..6446
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    6601..6606
                     /Recognition_pattern="TAGGAG"
                     /annotation_group="TAGGAG"
                     /label="Bce3081I"
                     /note="Geneious type: restriction site"
     misc_feature    6656..6661
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII, BfrI, BspTI, Bst98I, BstAFI, BstPZ740I,
                     Esp4I, MspCI, Vha464I"
                     /note="Geneious type: restriction site"
     misc_feature    6656..6661
                     /Recognition_pattern="CTTAAG"
                     /annotation_group="CTTAAG"
                     /label="BsaFI, BscLI, Cfr92I, TrsTII, Uba1266I, Uba1299I,
                     Uba1312I, Uba1313I, Uba1331I, Uba1374I, Uba1420I,
                     Uba1426I, Uba1443I, VfiI"
                     /note="Geneious type: restriction site"
     misc_feature    6656..6661
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII"
                     /note="Geneious type: restriction site"
     misc_feature    6668..6670
                     /ApEinfo_fwdcolor="#80ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Catalytic Serine"
     misc_feature    6800..6805
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="FauNDI, NdeI, PfaAII"
                     /note="Geneious type: restriction site"
     misc_feature    6800..6805
                     /Recognition_pattern="CATATG"
                     /annotation_group="CATATG"
                     /label="TrsKTIII"
                     /note="Geneious type: restriction site"
     misc_feature    6827..7525
                     /SerialCloner_Color="&h1D7D29"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="tagBFP"
     misc_feature    7450..7459
                     /Recognition_pattern="GACNN^NNGTC"
                     /annotation_group="GACNNNNGTC"
                     /label="BoxI, BstPAI, PshAI"
                     /note="Geneious type: restriction site"
     misc_feature    7547..11650
                     /SerialCloner_Color="&h5E06C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Sp dCas9"
     misc_feature    complement(8153..8164)
                     /Recognition_pattern="CACNNNNNTGGC"
                     /annotation_group="CACNNNNNTGGC"
                     /label="Eco912I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(9454..9466)
                     /Recognition_pattern="GGCANNNNNNTTC"
                     /annotation_group="GGCANNNNNNTTC"
                     /label="KpnAI"
                     /note="Geneious type: restriction site"
     misc_feature    10923..10929
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI, SanDI, Sse1825I"
                     /note="Geneious type: restriction site"
     misc_feature    11041..11050
                     /Recognition_pattern="GATNNNNATC"
                     /annotation_group="GATNNNNATC"
                     /label="Bco631I, Bco63I, Bse631I, UbaN20I, UbaN6I"
                     /note="Geneious type: restriction site"
     misc_feature    11041..11050
                     /Recognition_pattern="GATNN^NNATC"
                     /annotation_group="GATNNNNATC"
                     /label="BsaBI, Bse8I, BseJI, Bsh1365I, BsiBI, BsrBRI,
                     MamI"
                     /note="Geneious type: restriction site"
     misc_feature    11654..11680
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="HA tag"
     misc_feature    11699..11719
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SV40 NLS"
     misc_feature    11726..11746
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SV40 NLS(1)"
     misc_feature    11776..11783
                     /Recognition_pattern="VC^TCGAGB"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    11777..11782
                     /Recognition_pattern="CTC^GAG"
                     /annotation_group="CTCGAG"
                     /label="SciI"
                     /note="Geneious type: restriction site"
     misc_feature    11777..11782
                     /Recognition_pattern="CTCGAG"
                     /annotation_group="CTCGAG"
                     /label="AehI, AerAI, AhyAI, AlwFII, Asp15I, Asp47I,
                     Asp703I, BadI, BbfI, BbiIII, Bsp129I, Bsp139I, Bsp140I,
                     Bsp141I, Bsp142I, Bsp92I, BstHI, BstLI, BsuMI, BthI, CjaI,
                     DdeII, DrdDI, Eae2I, McaI, MecI, MhaI, MpuI, MrhI, MscAI,
                     MsiI, Msp23II, OcoI, Pfl67I, PflNI, PflWI, Psp4I, PspNI,
                     R1.BsuMI, R2.BsuMI, R3.BsuMI, Sal1974I, Sca1827I,
                     Sci1831I, SciBI, ScuI, SdiAI, SexI, Sfu1762I, SgaI, SgoI,
                     Sgr1841I, Shy1766I, SluI, SpaI, Sph1719I, SrifpI, Ssp4I,
                     StaAI, Sve194I, Uba1130I, Uba1148I, Uba1154I, Uba1166I,
                     Uba1237I, Uba1248I, Uba1271I, Uba1298I, Uba1335I,
                     Uba1397I, Uba1448I"
                     /note="Geneious type: restriction site"
     misc_feature    11777..11782
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="AbrI, BluI, BspAAI, BssHI, BstVI, CcrI, EscI,
                     MavI, MlaAI, PaeR7I, PanI, Sau3239I, SauLPII, Sbi68I,
                     Sfr274I, SlaI, Sol10179I, StrI, TliI, XhoI, XpaI"
                     /note="Geneious type: restriction site"
     promoter        11788..12035
                     /gene="3' PB"
                     /note="PROMOTER 3' PB repeat"
                     /note="3' PB repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="3' PB"
     misc_feature    12046..12053
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="CciNI, CsiBI, CspBI, MchAI, NotI"
                     /note="Geneious type: restriction site"
     misc_feature    12054..12061
                     /Recognition_pattern="TTAAT^TAA"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     promoter        complement(12076..12094)
                     /gene="T3p"
                     /note="PROMOTER T3 promoter promoter for bacteriophage T3
                     R NA polymerase"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T3p"
     misc_feature    complement(12115..12131)
                     /gene="M13R"
                     /note="M13 rev common sequencing primer, one of multiple
                     si milar variants"
                     /note="common sequencing primer, one of multiple similar
                     va riants"
                     /ApEinfo_fwdcolor="#cd3249"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13R"
     prot_bind       12139..12155
                     /gene="LacI"
                     /note="PROTEIN_BIND lac repressor encoded by lacI lac
                     opera tor The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved b y adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhib it transcription in E. coli. This inhibition can be
                     relieve d by adding lactose or
                     isopropyl-beta-D-thiogalactopyranosi de (IPTG)."
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LacI"
     promoter        complement(12163..12193)
                     /gene="LACp"
                     /note="PROMOTER lac promoter promoter for the E. coli lac
                     o peron"
                     /note="promoter for the E. coli lac operon"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LACp"
     prot_bind       12208..12229
                     /gene="CAPbs"
                     /note="PROTEIN_BIND E. coli catabolite activator protein
                     CA P binding site CAP binding activates transcription in
                     the p resence of cAMP."
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presenceof cAMP."
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CAPbs"
     misc_feature    12333..12339
                     /Recognition_pattern="GCTCTTC"
                     /annotation_group="GCTCTTC"
                     /label="Sgr13350III"
                     /note="Geneious type: restriction site"
     misc_feature    12333..12339
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="BspQI, LguI, PciSI, SapI, VpaK32I"
                     /note="Geneious type: restriction site"
     rep_origin      complement(12517..13105)
                     /gene="ORI"
                     /note="REP_ORIGIN ori high-copy-number
                     ColE1/pMB1/pBR322/pU C origin of replication"
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     rep lication"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ORI"
     CDS             complement(13276..14136)
                     /gene="AmpR"
                     /note="CDS beta-lactamase AmpR confers resistance to
                     ampici llin, carbenicillin, and related antibiotics"
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGAS LIKHW"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="beta-lactamase"
     promoter        complement(14137..14241)
                     /gene="AmpRp"
                     /note="PROMOTER AmpR promoter"
                     /note="AmpR promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpRp"
     rep_origin      complement(14267..14722)
                     /gene="ORI"
                     /note="REP_ORIGIN f1 ori f1 bacteriophage origin of
                     replica tion; arrow indicates direction of (+) strand
                     synthesis"
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indica tes direction of (+) strand synthesis"
                     /ApEinfo_label="ORI"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ORI(1)"
     misc_feature    14864..14880
                     /gene="M13F"
                     /note="M13 fwd common sequencing primer, one of multiple
                     si milar variants"
                     /note="common sequencing primer, one of multiple similar
                     va riants"
                     /ApEinfo_fwdcolor="#cd3249"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="M13F"
     misc_feature    complement(14870..14882)
                     /Recognition_pattern="TCANNNNNNGTCG"
                     /annotation_group="TCANNNNNNGTCG"
                     /label="EcoDR2"
                     /note="Geneious type: restriction site"
     promoter        14890..14908
                     /gene="T7p"
                     /note="PROMOTER T7 promoter promoter for bacteriophage T7
                     R NA polymerase"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="T7p"
     misc_feature    14918..14925
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     promoter        14934..15255
                     /gene="5' PB"
                     /note="PROMOTER 5' PB repeat"
                     /note="5' PB repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="5' PB"
     misc_feature    complement(15073..15079)
                     /Recognition_pattern="CRTTGAC(21/19)"
                     /annotation_group="CRTTGAC"
                     /label="MaqI"
                     /note="Geneious type: restriction site"
     misc_feature    15256..15261
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="AsuNHI, LlaG2I, NheI, PstNHI"
                     /note="Geneious type: restriction site"
     misc_feature    15256..15261
                     /Recognition_pattern="GCTAG^C"
                     /annotation_group="GCTAGC"
                     /label="AceII, BmtI, BspOI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
       61 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      121 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      181 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      241 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg
      301 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      361 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      421 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
      481 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
      541 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
      601 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
      661 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
      721 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
      781 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
      841 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
      901 cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
      961 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     1021 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     1081 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     1141 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     1201 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     1261 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1321 ggttattgtg ctgtctcatc attttggcaa agaattcgcc accatgtctt ccgacgaaga
     1381 agaagcgcgc gagctgatcg agcgtgcgaa ggaggctgcg gaacgcgcgc aagaagctgc
     1441 tgagcgtacc ggtgatccgc gcgtgcgtga actcgctcgt gagctgaaac gcctggctca
     1501 ggaagctgct gaagaagtga agcgtgaccc gtcttcctcc gatgtgaacg aagcgctcaa
     1561 actcatcgtg gaagctatcg aggcggctgt ggacgcgctc gaagcggctg aacgcaccgg
     1621 cgacccggag gttcgtgagc tggcgcgtga gctcgtgcgc ctcgcggttg aggctgcgga
     1681 ggaggtgcaa cgtaacccat cctcctctga cgtgaatgag gctctccaca gcatagttta
     1741 cgctattgaa gctgccattt ttgcattgga agctgccgaa aggactggtg acccggaagt
     1801 gagagaactc gcccgagagc ttgtaagact ggcggttgag gcggctgaag aagtccagcg
     1861 aaatccgtcc agccggaatg tcgagcacgc actgatgagg attgtgttgg ccatttacct
     1921 ggcagaagaa aatctccgcg aagctgagga gtctggcgat ccggaaaaac gtgaaaaggc
     1981 tcgcgaacgt gttcgtgagg cggtggaacg tgcggaagag gttcagcgtg atccatccgg
     2041 ttggctgaat catgaacaaa aacttatttc cgaagaggat ctggaattct caagcgctgc
     2101 aggtactagt gatgctttag acgattttga cttagatatg cttggttcag acgcgttaga
     2161 cgacttcgac ctagacatgt taggctcaga tgcattggac gacttcgatt tagatatgtt
     2221 gggctccgat gccctagatg actttgatct agatatgcta ggtagtccca aaaagaagag
     2281 gaaagtggga tcccagtatc tgcccgacac agatgataga caccgaatcg aagagaaacg
     2341 caagcgaacg tatgaaacct tcaaatcgat catgaagaaa tcgcccttct cgggtccgac
     2401 cgatcccagg cccccaccga gaaggattgc ggtcccgtcc cgctcgtcgg ccagcgtgcc
     2461 gaagcctgcg ccgcagccct accccttcac gtcgagcctg agcacaatca attatgacga
     2521 gttcccgacg atggtgttcc cctcgggaca aatctcacaa gcctcggcgc tcgcaccagc
     2581 gcctccccaa gtccttccgc aagcgcctgc cccagcgcct gcaccggcaa tggtgtccgc
     2641 cctcgcacag gcccctgcgc ccgtccccgt gctcgcgcct ggaccgcccc aggcggtcgc
     2701 tccaccggct ccgaagccga cgcaggccgg agagggaaca ctctccgaag cacttcttca
     2761 actccagttt gatgacgagg atcttggagc actccttgga aactcgacag accctgcggt
     2821 gtttaccgac ctcgcgtcag tagataactc cgaatttcag cagcttttga accagggtat
     2881 cccggtcgcg ccacatacaa cggagcccat gttgatggaa taccccgaag caatcacgag
     2941 acttgtgacg ggagcgcagc ggcctcccga tcccgcaccc gcacctttgg gggcacctgg
     3001 cctccctaac ggacttttga gcggcgacga ggatttctcc tccatcgccg atatggattt
     3061 ctcagccttg ctgtcacaga tttccagcgg ctctggcagc ggcagccggg attccaggga
     3121 agggatgttt ttgccgaagc ctgaggccgg ctccgctatt agtgacgtgt ttgagggccg
     3181 cgaggtgtgc cagccaaaac gaatccggcc atttcatcct ccaggaagtc catgggccaa
     3241 ccgcccactc cccgccagcc tcgcaccaac accaaccggt ccagtacatg agccagtcgg
     3301 gtcactgacc ccggcaccag tccctcagcc actggatcca gcgcccgcag tgactcccga
     3361 ggccagtcac ctgttggagg atcccgatga agagacgagc caggctgtca aagcccttcg
     3421 ggagatggcc gatactgtga ttccccagaa ggaagaggct gcaatctgtg gccaaatgga
     3481 cctttcccat ccgcccccaa ggggccatct ggatgagctg acaaccacac ttgagtccat
     3541 gaccgaggat ctgaacctgg actcacccct gaccccggaa ttgaacgaga ttctggatac
     3601 cttcctgaac gacgagtgcc tcttgcatgc catgcatatc agcacaggac tgtccatctt
     3661 cgacacatct ctgttttgaa tttaaataat tcgcccctct ccctcccccc cccctaacgt
     3721 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3781 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3841 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     3901 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     3961 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4021 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4081 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4141 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacgtgtg tttagtcgag
     4201 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4261 tgataatatg gccacaaccg gatccggagc taccatgacc atgaccgagt acaagcccac
     4321 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4381 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4441 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4501 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4561 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4621 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4681 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4741 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4801 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4861 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtttaaacca tgcaagcttg
     4921 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4981 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5041 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5101 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5161 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5221 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5281 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5341 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5401 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5461 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5521 ccccgcatcc ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5641 tgtatcttat catgtctgga tcctgcaggt aattctaccg ggtaggggag gcgcttttcc
     5701 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     5761 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     5821 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     5881 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagg ctcgtgcaga
     5941 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     6001 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     6061 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     6121 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     6181 cctgcagccc aagcttgtcg acgccaccat gaaaaagaaa ggttctgttg tcatcgttgg
     6241 acgcataaat ctctctggcg acaccgcata ttctcagcag acgcgaggtc tggaagggtg
     6301 tcaagagacc tcacaaaccg ggagggacaa gaatcaagta gaaggggagg tccaggtggt
     6361 atctacggca acccaatcct tcttggcgac atctatcaat ggagtcctgt ggacggttta
     6421 tcatggtgcg ggcactcgga caattgcctc tccaaaaggt ccagttaccc agatgtatac
     6481 taatgtagac aaagatcttg tcggatggca agcaccacaa gggtctcgga gcctcactcc
     6541 ctgtacgtgt ggaagttcag acctttatct cgtaactaga cacgcggatg ttatccccgt
     6601 taggagaaga ggagatagta ggggatccct gctttcacca cgcccaatat cctatcttaa
     6661 gggttcttca ggtggtccct tgttgtgtcc ggcgggtcac gcggtaggta tattccgcgc
     6721 ggccgtatct actcggggcg tggctaaggc ggtcgatttc ataccggttg agtccttgga
     6781 aacaacgatg cggagccccc atatgtcaag cgctgcaggt gctaccatga gcgagctgat
     6841 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     6901 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     6961 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     7021 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     7081 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     7141 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     7201 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     7261 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     7321 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     7381 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     7441 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     7501 tagcaaactg gggcacaagc ttaattcaag cgctgcaggt gccaccatgg acaagaagta
     7561 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
     7621 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
     7681 gaacctgatc ggcgccctgc tgttcgacag cggagaaaca gccgaggcca cccggctgaa
     7741 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
     7801 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
     7861 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
     7921 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
     7981 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
     8041 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
     8101 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
     8161 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
     8221 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag
     8281 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
     8341 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
     8401 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
     8461 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
     8521 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
     8581 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acatcgatgg
     8641 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
     8701 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
     8761 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
     8821 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
     8881 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
     8941 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
     9001 cgccagcgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
     9061 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtaca acgagctgac
     9121 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
     9181 aaaagccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
     9241 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
     9301 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
     9361 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
     9421 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
     9481 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
     9541 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
     9601 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
     9661 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
     9721 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
     9781 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
     9841 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
     9901 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
     9961 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
    10021 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag
    10081 ctttctgaag gacgactcca tcgataacaa agtgctgact cggagcgaca agaaccgggg
    10141 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcgcca
    10201 gctgctgaat gccaagctga ttacccagag gaagttcgac aatctgacca aggccgagag
    10261 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
    10321 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
    10381 gaacgacaaa ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
    10441 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
    10501 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
    10561 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
    10621 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
    10681 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
    10741 aaacggcgaa acaggcgaga tcgtgtggga taagggccgg gactttgcca ccgtgcggaa
    10801 agtgctgtct atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
    10861 cagcaaagag tctatcctgc ccaagaggaa cagcgacaag ctgatcgcca gaaagaagga
    10921 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
    10981 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg
    11041 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
    11101 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
    11161 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
    11221 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
    11281 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaaacacta
    11341 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
    11401 taatctggac aaggtgctga gcgcctacaa caagcacaga gacaagccta tcagagagca
    11461 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
    11521 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
    11581 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
    11641 gggaggcgac gcctatccct atgacgtgcc cgattatgcc agcctgggca gcggctcccc
    11701 caagaaaaaa cgcaaggtgg aagatcctaa gaaaaagcgg aaagtggacg gcattggtag
    11761 tgggagcaac ggatgactcg agctagttaa aagttttgtt actttataga agaaattttg
    11821 agtttttgtt tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta
    11881 ttagtatgta agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct
    11941 cgatatacag accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg
    12001 tcacaatatg attatctttc tagggttaat ctagtatacg cgtatgcggc cgcttaatta
    12061 atccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
    12121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    12181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    12241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    12301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    12361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    12421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    12481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    12541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    12601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    12661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    12721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    12961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    13021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    13081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    13141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    13201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    13261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    13321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    13381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    13441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    13501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    13561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    13621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    14041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    14101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    14161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    14221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
    14281 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
    14341 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
    14401 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
    14461 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
    14521 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
    14581 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
    14641 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
    14701 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
    14761 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    14821 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    14881 gagcgcgcgt aatacgactc actatagggc gaattggggc gcgccattct agattaaccc
    14941 tagaaagata gtctgcgtaa aattgacgca tgcattcttg aaatattgct ctctctttct
    15001 aaatagcgcg aatccgtcgc tgtgcattta ggacatctca gtcgccgctt ggagctcccg
    15061 tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg aactataacg accgcgtgag
    15121 tcaaaatgac gcatgattat cttttacgtg acttttaaga tttaactcat acgataatta
    15181 tattgttatt tcatgttcta cttacgtgat aacttattat atatatattt tcttgttata
    15241 gatatcaact agaatgctag catgggccca gatct
//
