LOCUS       GWF314                  8718 bp ds-DNA     circular     15-SEP-2019
DEFINITION  CXCR4_6_1Xcom__com-GNCR1-P2a-BFP.
ACCESSION   urn.local...u-aykyd2n
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     New DNA(6) from 1 to 8308 New DNA(5) from 1 to 8308 New DNA(4) from
            1 to 8308 New DNA(3) from 1 to 8308 New DNA(2) from 1 to 8308 New
            DNA(1) from 1 to 8308 U6_sgRNA_template from 1 to 8308 pMig-w from
            1 to 6471 ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    263..826
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pU6 (pMJ177) 5' CMV promoter IE"
     misc_feature    844..1024
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV-1_5_LTR"
     misc_feature    845..850
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site"
     misc_feature    complement(977..982)
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="XbaI-SpeI scar"
     misc_feature    982..987
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar"
     misc_feature    complement(982..987)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(5)"
     misc_feature    complement(1018..1023)
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(4)"
     misc_feature    complement(1155..1160)
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar(2)"
     misc_feature    1165..1170
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar"
     misc_feature    1301..1306
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(1)"
     misc_feature    1695..1928
                     /ApEinfo_fwdcolor="#9cffe3"
                     /ApEinfo_revcolor="#9cffe3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="RRE"
     misc_feature    1984..1989
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar"
     misc_feature    2380..2385
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(1)"
     misc_feature    complement(2380..2385)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(6)"
     misc_feature    2459..2473
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="cPPT"
     misc_feature    2596..2601
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(2)"
     misc_feature    complement(2596..2601)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(7)"
     misc_feature    2630..2635
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(3)"
     misc_feature    complement(2630..2635)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(8)"
     misc_feature    2631..2933
                     /ApEinfo_fwdcolor="#fff81f"
                     /ApEinfo_revcolor="#fff81f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="U6 promoter"
     misc_feature    complement(2790..2795)
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar(3)"
     misc_recomb     2825..2858
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LoxP recombination site"
     misc_feature    2945..2967
                     /ApEinfo_fwdcolor="#ffb005"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CXCR4-6"
     misc_feature    2946..2967
                     /Original_Bases="TACGTTCTCTATCACTGATA"
                     /label="TACGTTCTCTATCACTGATA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2968..3053
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="modified stem loop"
     misc_feature    3054..3072
                     /ApEinfo_fwdcolor="#ff8f17"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="1Xcom"
     misc_feature    3073..3092
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SUP4 terminator"
     misc_feature    3093..3098
                     /ApEinfo_fwdcolor="#7bd5ff"
                     /ApEinfo_revcolor="#7bd5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="PstI"
     motif           3107..3112
                     /created_by="User"
                     /label="XhoI"
     misc_feature    3164..3746
                     /ApEinfo_fwdcolor="#ffed31"
                     /ApEinfo_revcolor="#ffed31"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMV promoter IE"
     misc_feature    3164..3727
                     /ApEinfo_label="pU6 (pMJ177) 5' CMV promoter IE"
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pU6 (pMJ177) 5' CMV promoter IE(1)"
     misc_feature    3748..3753
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(4)"
     misc_feature    complement(3748..3753)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(9)"
     misc_binding    3768..3788
                     /ApEinfo_fwdcolor="#ff7e07"
                     /ApEinfo_revcolor="#08b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Nuclear Localization Signal (from SV40)"
     misc_feature    3795..3980
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="com protein"
     misc_feature    3795..3980
                     /ApEinfo_fwdcolor="#ff68aa"
                     /ApEinfo_revcolor="#ff68aa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="COM protein"
     misc_feature    3987..3992
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    <3993..4001
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Linker"
     misc_feature    3995..5495
                     /Original_Bases="ACGAGCCGATGCTCTCGACGATTTTGACCTCGATATGCTGG
                     GATCCGACGCTCTCGATGACTTCGACCTTGACATGCTGGGGAGTGACGCTCTGGACGA
                     CTTCGATCTCGACATGCTGGGCTCCGACGCCCTGGATGACTTCGATCTGGACATGCTC
                     ATCAACTGACCCGGGTCGAGCATGCATCTAGGGCGGCCAATTCCGCCCCTCTCCCTCC
                     CCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCT
                     ATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGG
                     CCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAA
                     GGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAA
                     CGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTG
                     CGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCAC
                     GTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACA
                     AGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCG
                     GTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAAC
                     CACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAAATGGTGAGCAAGGGCGAGG
                     AGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTC
                     CGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGC
                     ACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACA
                     TCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACAT
                     CCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAAC
                     TTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGT
                     TCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCA
                     GAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCC
                     CTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTG
                     AGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGT
                     CAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTAC
                     GAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG"
                     /label="ACGAGCCGATGCTCTCGACGATTTTGACCTCGATATGCTGGGATCCGACG
                     CTCTCGATGACTTCGACCTTGACATGCTGGGGAGTGACGCTCTGGACGACTTCGATCT
                     CGACATGCTGGGCTCCGACGCCCTGGATGACTTCGATCTGGACATGCTCATCAACTGA
                     CCCGGGTCGAGCATGCATCTAGGGCGGCCAATTCCGCCCCTCTCCCTCCCCCCCCCCT
                     AACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTAT
                     TTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTT
                     CTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTG
                     AATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAG
                     CGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAA
                     GCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGT
                     TGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGA
                     AGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATG
                     CTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGAC
                     GTGGTTTTCCTTTGAAAAACACGATGATAAATGGTGAGCAAGGGCGAGGAGGATAACA
                     TGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGG
                     CCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACC
                     GCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCC
                     CTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTA
                     CTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGAC
                     GGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACA
                     AGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGAC
                     CATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGC
                     GAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGA
                     CCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAA
                     GTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCC
                     GAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4002..4700
                     /created_by="User"
                     /modified_by="User"
                     /label="GNCR1"
     misc_feature    4701..4730
                     /ApEinfo_fwdcolor="#ccff66"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Myc Tag_C-Term"
     gene            <4731..>4796
                     /created_by="User"
                     /modified_by="User"
                     /label="P2a"
     gene            <4797..5492
                     /created_by="User"
                     /label="BFP"
     misc_recomb     5518..5551
                     /ApEinfo_label="LoxP recombination site"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LoxP recombination site(1)"
     misc_feature    5597..6197
                     /ApEinfo_fwdcolor="#0060ff"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="wPRE"
     misc_feature    complement(6169..6174)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(9)"
     misc_feature    6169..6174
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar"
     misc_feature    complement(6218..6223)
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site(2)"
     misc_feature    6285..6290
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(2)"
     misc_feature    6387..6401
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="cPPT(1)"
     misc_feature    complement(6452..6457)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(10)"
     misc_feature    6452..6457
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(1)"
     misc_feature    6546..6551
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar(1)"
     misc_feature    6724..6904
                     /ApEinfo_label="HIV-1_5_LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV-1_5_LTR(1)"
     misc_feature    6725..6730
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site(1)"
     misc_feature    complement(6857..6862)
                     /ApEinfo_label="XbaI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="XbaI-SpeI scar(1)"
     misc_feature    6862..6867
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(5)"
     misc_feature    complement(6862..6867)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(10)"
     misc_feature    complement(6898..6903)
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(5)"
     rep_origin      complement(6948..7630)
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1 origin"
     misc_feature    complement(6951..7570)
                     /ApEinfo_fwdcolor="#95928e"
                     /ApEinfo_revcolor="#95928e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pBR322_origin"
     misc_feature    complement(7399..7404)
                     /ApEinfo_label="XbaI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="XbaI-SpeI scar(2)"
     misc_feature    complement(7546..7551)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(11)"
     misc_feature    7546..7551
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(2)"
     misc_feature    7557..7562
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(6)"
     misc_feature    complement(7557..7562)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(11)"
     misc_feature    complement(7643..7648)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(12)"
     misc_feature    7643..7648
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(3)"
     misc_feature    7655..7660
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(7)"
     misc_feature    complement(7655..7660)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(12)"
     CDS             complement(7728..8387)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="AmpR"
     misc_feature    complement(7865..7870)
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site(3)"
     misc_feature    7987..7992
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(3)"
     misc_feature    complement(8423..8428)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(13)"
     misc_feature    8423..8428
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(4)"
     misc_feature    8440..8445
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(8)"
     misc_feature    complement(8440..8445)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(13)"
     misc_feature    complement(8627..8655)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="AmpR promoter"
ORIGIN      
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttggcagac gcgaggaagg agggcgcgtt taagagctat gctggaaaca gcatagcaag
     3001 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcctgaatg
     3061 cctgcgagca tctttttttg ttttttatgt ctctgcagag ttcggtctcg agtactagga
     3121 tccattaggc ggccgcgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     3181 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3541 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3661 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3721 taagcagagc tggtttagtg aaccgtcaga tccgctagcc caccatgccc aaaaagaaaa
     3781 gaaaagtggg tagtatgaaa tcaattcgct gtaaaaactg caacaaactg ttatttaagg
     3841 cggatagttt tgatcacatt gaaatcaggt gtccgcgttg caaacgtcac atcataatgc
     3901 tgaatgcctg cgagcatccc acggagaaac attgtgggaa aagagaaaaa atcacgcatt
     3961 ctgacgaaac cgtgcgttat ggctccggta gcggctctgg tgatatcgag aagctttgca
     4021 aaaaagctga agaagaggca aaggaggcgc aagagaaagc tgatgagttg cggcaacggc
     4081 accccgacag ccaggctgct gaggatgctg aggacttggc aaatctggcg gtggccgctg
     4141 tcttgacggc ttgcttgttg gcccaagaac atccaaacgc tgacattgca aagctttgca
     4201 ttaaggccgc gtccgaagcg gctgaagcag cttctaaggc agccgagctg gcgcaacggc
     4261 atcctgactc ccaagcggcg agggacgcta taaaactcgc atcacaagca gccagagctg
     4321 taatcctcgc tattatgctc gcagccgaaa acccaaacgc tgatattgcc aaactgtgta
     4381 taaaggcggc tagtgaagcc gcagaagctg caagcaaagc agcagaactt gcccaacgcc
     4441 accccgactc ccaggcggct agagatgcta taaaacttgc ttctcaggct gcggaggcgg
     4501 tagaacgagc gatctggctg gcagcagaga accccaacgc tgacattgcc aaaaagtgta
     4561 tcaaggcggc ttccgaagcc gccgaggagg ccagcaaagc ggcggaagag gcacagagac
     4621 atccggactc acagaaagca cgcgacgaga ttaaagaggc tagtcaaaaa gcagaggagg
     4681 taaaagaacg ctgtaagtcc gagcaaaaac tcatatctga ggaggacttg ggaagcggag
     4741 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctagcg
     4801 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     4861 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     4921 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     4981 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     5041 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     5101 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     5161 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     5221 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     5281 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     5341 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     5401 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     5461 acctccctag caaactgggg cacaagctta attgataaga attcgtcgag ggacctaata
     5521 acttcgtata gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg
     5581 tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     5641 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5701 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5761 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5821 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5881 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5941 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6001 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6061 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6121 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6181 gggccgcctc cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca
     6241 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     6301 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     6361 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6421 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     6481 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     6541 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     6601 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     6661 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     6721 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6781 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6841 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6901 agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7381 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7441 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7501 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7561 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7621 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7681 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7741 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7801 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7861 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7921 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7981 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8041 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8101 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8161 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8221 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8281 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8341 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8401 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8461 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8521 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8581 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8641 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8701 cgaaaagtgc cacctgac
//
