LOCUS       GWF303                  8938 bp ds-DNA     circular     15-SEP-2019
DEFINITION  CD95-1_2XPP7__PCP-DNCR2-P2a-BFP.
ACCESSION   urn.local...t-aykyd2m
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     New DNA(6) from 1 to 8308 New DNA(5) from 1 to 8308 New DNA(4) from
            1 to 8308 New DNA(3) from 1 to 8308 New DNA(2) from 1 to 8308 New
            DNA(1) from 1 to 8308 U6_sgRNA_template from 1 to 8308 pMig-w from
            1 to 6471 ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    10..15
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    173..178
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    240..245
                     /Recognition_pattern="A^CGCGT"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     misc_feature    261..266
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    263..826
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pU6 (pMJ177) 5' CMV promoter IE"
     misc_feature    495..500
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    600..605
                     /Recognition_pattern="TAC^GTA"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    600..605
                     /Recognition_pattern="YAC^GTR"
                     /annotation_group="YACGTR"
                     /label="BsaAI, BstBAI, Ppu21I"
                     /note="Geneious type: restriction site"
     misc_feature    622..627
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI, Bsp19I"
                     /note="Geneious type: restriction site"
     misc_feature    650..661
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     misc_feature    844..1024
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV-1_5_LTR"
     misc_feature    845..850
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site"
     misc_feature    complement(977..982)
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="XbaI-SpeI scar"
     misc_feature    982..987
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar"
     misc_feature    complement(982..987)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(5)"
     misc_feature    complement(1018..1023)
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(4)"
     misc_feature    1027..1032
                     /Recognition_pattern="GGC^GCC"
                     /annotation_group="GGCGCC"
                     /label="DinI, EgeI, EheI, SfoI"
                     /note="Geneious type: restriction site"
     misc_feature    1027..1032
                     /Recognition_pattern="GGCGC^C"
                     /annotation_group="GGCGCC"
                     /label="PluTI"
                     /note="Geneious type: restriction site"
     misc_feature    1027..1032
                     /Recognition_pattern="GG^CGCC"
                     /annotation_group="GGCGCC"
                     /label="NarI, Mly113I"
                     /note="Geneious type: restriction site"
     misc_feature    1027..1032
                     /Recognition_pattern="G^GCGCC"
                     /annotation_group="GGCGCC"
                     /label="KasI, SspDI"
                     /note="Geneious type: restriction site"
     misc_feature    1065..1075
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /annotation_group="GAGNNNNNCTC"
                     /label="BplI"
                     /note="Geneious type: restriction site"
     misc_feature    1101..1106
                     /Recognition_pattern="G^CGCGC"
                     /annotation_group="GCGCGC"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1155..1160)
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar(2)"
     misc_feature    1165..1170
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar"
     misc_feature    1301..1306
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(1)"
     misc_feature    complement(1497..1503)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    1535..1542
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    1558..1568
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    1581..1586
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    1695..1928
                     /ApEinfo_fwdcolor="#9cffe3"
                     /ApEinfo_revcolor="#9cffe3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="RRE"
     misc_feature    complement(1815..1821)
                     /Recognition_pattern="CCTCAGC(-5/-2)"
                     /annotation_group="CCTCAGC"
                     /label="BbvCI"
                     /note="Geneious type: restriction site"
     misc_feature    1839..1849
                     /Recognition_pattern="GCANNNN^NTGC"
                     /annotation_group="GCANNNNNTGC"
                     /label="BstAPI"
                     /note="Geneious type: restriction site"
     misc_feature    1965..1974
                     /Recognition_pattern="CACNN^NNGTG"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    1980..1985
                     /Recognition_pattern="GAATGC(1/-1)"
                     /annotation_group="GAATGC"
                     /label="BsmI, Mva1269I, PctI"
                     /note="Geneious type: restriction site"
     misc_feature    1984..1989
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar"
     misc_feature    complement(2218..2228)
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /annotation_group="ACNNNNNCTCC"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     misc_feature    2325..2331
                     /Recognition_pattern="RG^GWCCY"
                     /annotation_group="RGGWCCY"
                     /label="PpuMI, Psp5II, PspPPI"
                     /note="Geneious type: restriction site"
     misc_feature    2325..2331
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2325..2331
                     /Recognition_pattern="RG^GNCCY"
                     /annotation_group="RGGNCCY"
                     /label="EcoO109I"
                     /note="Geneious type: restriction site"
     misc_feature    2380..2385
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(1)"
     misc_feature    complement(2380..2385)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(6)"
     misc_feature    2407..2417
                     /Recognition_pattern="GCANNNN^NTGC"
                     /annotation_group="GCANNNNNTGC"
                     /label="BstAPI"
                     /note="Geneious type: restriction site"
     misc_feature    2425..2430
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI, BspMAI"
                     /note="Geneious type: restriction site"
     misc_feature    2459..2473
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="cPPT"
     misc_feature    2596..2601
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(2)"
     misc_feature    complement(2596..2601)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(7)"
     misc_feature    2617..2622
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2617..2622
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    2621..2626
                     /Recognition_pattern="CCGCTC(-3/-3)"
                     /annotation_group="CCGCTC"
                     /label="BsrBI, AccBSI, MbiI"
                     /note="Geneious type: restriction site"
     misc_feature    2625..2630
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    2630..2635
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(3)"
     misc_feature    complement(2630..2635)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(8)"
     misc_feature    2631..2933
                     /ApEinfo_fwdcolor="#fff81f"
                     /ApEinfo_revcolor="#fff81f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="U6 promoter"
     misc_feature    2686..2698
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    2725..2730
                     /Recognition_pattern="AAT^ATT"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2790..2795)
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar(3)"
     misc_recomb     2825..2858
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LoxP recombination site"
     misc_feature    2855..2860
                     /Recognition_pattern="TTA^TAA"
                     /annotation_group="TTATAA"
                     /label="PsiI, AanI"
                     /note="Geneious type: restriction site"
     misc_RNA        2945..2965
                     /created_by="User"
                     /label="CD95-1"
     misc_feature    2949..2964
                     /Original_Bases="GTTCTCTATCACTGAT"
                     /label="GTTCTCTATCACTGAT"
                     /note="Geneious type: Editing History Replacement"
     ORF             <2966..3130
                     /created_by="User"
                     /label="Insert1-scRNA-2xPP7"
     misc_feature    2966..3051
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="modified stem loop"
     misc_feature    3052..3130
                     /ApEinfo_fwdcolor="#ffa30e"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="2XPP7_ds3"
     misc_feature    3131..3150
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SUP4 terminator"
     misc_feature    3151..3156
                     /ApEinfo_fwdcolor="#7bd5ff"
                     /ApEinfo_revcolor="#7bd5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="PstI"
     misc_feature    3151..3156
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI, BspMAI"
                     /note="Geneious type: restriction site"
     misc_feature    3162..3164
                     /ApEinfo_fwdcolor="#7bd5ff"
                     /ApEinfo_revcolor="#7bd5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Acc65I"
     misc_feature    3165..3170
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
                     /note="Geneious type: restriction site"
     misc_feature    3169..3174
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    3176..3181
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    3187..3194
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    3216..3221
                     /Recognition_pattern="ATGCA^T"
                     /annotation_group="ATGCAT"
                     /label="EcoT22I, Mph1103I, NsiI, Zsp2I"
                     /note="Geneious type: restriction site"
     misc_feature    3222..3804
                     /ApEinfo_fwdcolor="#ffed31"
                     /ApEinfo_revcolor="#ffed31"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="CMV promoter IE"
     misc_feature    3222..3785
                     /ApEinfo_label="pU6 (pMJ177) 5' CMV promoter IE"
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pU6 (pMJ177) 5' CMV promoter IE(1)"
     misc_feature    3454..3459
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    3559..3564
                     /Recognition_pattern="TAC^GTA"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    3559..3564
                     /Recognition_pattern="YAC^GTR"
                     /annotation_group="YACGTR"
                     /label="BsaAI, BstBAI, Ppu21I"
                     /note="Geneious type: restriction site"
     misc_feature    3581..3586
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI, Bsp19I"
                     /note="Geneious type: restriction site"
     misc_feature    3609..3620
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     primer_bind     3787..3811
                     /Sequence="GCTGGTTTAGTGAACCGTCAGATCC"
                     /Hairpin_Tm="57.3"
                     /Tm="63.6"
                     /Self_Dimer_Tm="5.7"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="SecondInsert_seq"
     misc_feature    3806..3811
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(4)"
     misc_feature    complement(3806..3811)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(9)"
     misc_feature    3812..3817
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI, AsuNHI"
                     /note="Geneious type: restriction site"
     misc_feature    3812..3817
                     /Recognition_pattern="GCTAG^C"
                     /annotation_group="GCTAGC"
                     /label="BmtI, BspOI"
                     /note="Geneious type: restriction site"
     ORF             3823..>4224
                     /created_by="User"
                     /label="Insert2-PCP-VP64"
     misc_binding    3826..3846
                     /ApEinfo_fwdcolor="#ff7e07"
                     /ApEinfo_revcolor="#08b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Nuclear Localization Signal (from SV40)"
     misc_feature    3853..4218
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="PP7delFG"
     misc_feature    3934..3939
                     /Recognition_pattern="TT^CGAA"
                     /annotation_group="TTCGAA"
                     /label="AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV,
                     SfuI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3936..3948)
                     /Recognition_pattern="(8/13)GACNNNNNNTTYG(11/6)"
                     /annotation_group="GACNNNNNNTTYG"
                     /label="ArsI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4096..4101)
                     /Recognition_pattern="CACGTC(-3/-3)"
                     /annotation_group="CACGTC"
                     /label="BtrI, AjiI, BmgBI"
                     /note="Geneious type: restriction site"
     misc_feature    4124..4129
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI, Eco147I, PceI, SseBI"
                     /note="Geneious type: restriction site"
     misc_feature    4152..4160
                     /Recognition_pattern="GACN^NNGTC"
                     /annotation_group="GACNNNGTC"
                     /label="Tth111I, PflFI, PsyI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4176..4181)
                     /Recognition_pattern="ACCTGC(4/8)"
                     /annotation_group="ACCTGC"
                     /label="BspMI, Acc36I, BfuAI, BveI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4182..4194)
                     /Recognition_pattern="(8/13)GACNNNNNNTTYG(11/6)"
                     /annotation_group="GACNNNNNNTTYG"
                     /label="ArsI"
                     /note="Geneious type: restriction site"
     misc_feature    4225..4950
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    <4225..4233
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Linker"
     gene            4234..4920
                     /created_by="User"
                     /modified_by="User"
                     /label="DNCR2"
     misc_feature    4306..4312
                     /Recognition_pattern="GC^TNAGC"
                     /annotation_group="GCTNAGC"
                     /label="BlpI, Bpu1102I, Bsp1720I"
                     /note="Geneious type: restriction site"
     misc_feature    4315..4320
                     /Recognition_pattern="A^CCGGT"
                     /annotation_group="ACCGGT"
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     misc_feature    4359..4369
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    4419..4424
                     /Recognition_pattern="AGC^GCT"
                     /annotation_group="AGCGCT"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    4482..4489
                     /Recognition_pattern="CR^CCGGYG"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    4562..4572
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /annotation_group="ACNNNNNCTCC"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     misc_feature    4572..4593
                     /created_by="User"
                     /label="HSinsert_fwd"
     misc_feature    4622..4636
                     /Recognition_pattern="CCANNNNN^NNNNTGG"
                     /annotation_group="CCANNNNNNNNNTGG"
                     /label="XcmI"
                     /note="Geneious type: restriction site"
     misc_feature    4654..4660
                     /Recognition_pattern="G^GTNACC"
                     /annotation_group="GGTNACC"
                     /label="BstEII, BstPI, Eco91I, EcoO65I, PspEI"
                     /note="Geneious type: restriction site"
     misc_feature    4775..4780
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4801..4820)
                     /created_by="User"
                     /label="HSinsert_rev"
     misc_feature    complement(4810..4816)
                     /Recognition_pattern="CCTCAGC(-5/-2)"
                     /annotation_group="CCTCAGC"
                     /label="BbvCI"
                     /note="Geneious type: restriction site"
     misc_feature    4921..4950
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Myc Tag"
     gene            <4951..>5016
                     /created_by="User"
                     /modified_by="User"
                     /label="P2a"
     misc_feature    4952..4964
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    4954..5715
                     /Original_Bases="CGAGCCGATGCTCTCGACGATTTTGACCTCGATATGCTGGG
                     ATCCGACGCTCTCGATGACTTCGACCTTGACATGCTGGGGAGTGACGCTCTGGACGAC
                     TTCGATCTCGACATGCTGGGCTCCGACGCCCTGGATGACTTCGATCTGGACATGCTCA
                     TCAACTGACCCGGGTCGAGCATGCATCTAGGGCGGCCAATTCCGCCCCTCTCCCTCCC
                     CCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTA
                     TATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGC
                     CCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAG
                     GTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAAC
                     GTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGC
                     GGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACG
                     TTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAA
                     GGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGG
                     TGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACC
                     ACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAAATGGTGAGCAAGGGCGAGGA
                     GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCC
                     GTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCA
                     CCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACAT
                     CCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATC
                     CCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACT
                     TCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTT
                     CATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAG
                     AAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCC
                     TGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGA
                     GGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTC
                     AACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACG
                     AACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG"
                     /label="CGAGCCGATGCTCTCGACGATTTTGACCTCGATATGCTGGGATCCGACGC
                     TCTCGATGACTTCGACCTTGACATGCTGGGGAGTGACGCTCTGGACGACTTCGATCTC
                     GACATGCTGGGCTCCGACGCCCTGGATGACTTCGATCTGGACATGCTCATCAACTGAC
                     CCGGGTCGAGCATGCATCTAGGGCGGCCAATTCCGCCCCTCTCCCTCCCCCCCCCCTA
                     ACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATT
                     TTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTC
                     TTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGA
                     ATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGC
                     GACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAG
                     CCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTT
                     GGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAA
                     GGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGC
                     TTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACG
                     TGGTTTTCCTTTGAAAAACACGATGATAAATGGTGAGCAAGGGCGAGGAGGATAACAT
                     GGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGC
                     CACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCG
                     CCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCC
                     TCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTAC
                     TTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACG
                     GCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA
                     GGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACC
                     ATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCG
                     AGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGAC
                     CACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAG
                     TTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCG
                     AGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(4991..4996)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="Esp3I, BsmBI"
                     /note="Geneious type: restriction site"
     gene            <5017..5712
                     /created_by="User"
                     /label="BFP"
     misc_feature    5051..5056
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    5231..5237
                     /Recognition_pattern="CC^CWGGG"
                     /annotation_group="CCCWGGG"
                     /label="PasI"
                     /note="Geneious type: restriction site"
     misc_feature    5266..5272
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     misc_feature    5342..5348
                     /Recognition_pattern="T^CCNGGA"
                     /annotation_group="TCCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     misc_feature    5385..5397
                     /Recognition_pattern="(7/12)GAACNNNNNNTCC(12/7)"
                     /annotation_group="GAACNNNNNNTCC"
                     /label="AloI"
                     /note="Geneious type: restriction site"
     misc_feature    5435..5440
                     /Recognition_pattern="AGG^CCT"
                     /annotation_group="AGGCCT"
                     /label="StuI, Eco147I, PceI, SseBI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5446..5451)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="Esp3I, BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    5637..5646
                     /Recognition_pattern="GACNN^NNGTC"
                     /annotation_group="GACNNNNGTC"
                     /label="PshAI, BoxI, BstPAI"
                     /note="Geneious type: restriction site"
     misc_feature    5666..5671
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     misc_feature    5719..5724
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    5730..5736
                     /Recognition_pattern="RG^GWCCY"
                     /annotation_group="RGGWCCY"
                     /label="PpuMI, Psp5II, PspPPI"
                     /note="Geneious type: restriction site"
     misc_feature    5730..5736
                     /Recognition_pattern="RG^GNCCY"
                     /annotation_group="RGGNCCY"
                     /label="EcoO109I"
                     /note="Geneious type: restriction site"
     misc_recomb     5738..5771
                     /ApEinfo_label="LoxP recombination site"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="LoxP recombination site(1)"
     primer_bind     complement(5764..5790)
                     /Sequence="CCGGAACCCTTAAACATGTATAACTTC"
                     /Hairpin_Tm="None"
                     /Tm="60.2"
                     /Self_Dimer_Tm="None"
                     /%GC="40.7"
                     /created_by="primer3"
                     /label="SecondInsert_rev_seq"
     misc_feature    5772..5777
                     /Recognition_pattern="A^CATGT"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     misc_feature    5817..6417
                     /ApEinfo_fwdcolor="#0060ff"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="wPRE"
     misc_feature    5821..5826
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI"
                     /note="Geneious type: restriction site"
     misc_feature    6062..6072
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI, AccB7I, Van91I"
                     /note="Geneious type: restriction site"
     misc_feature    6100..6106
                     /Recognition_pattern="T^CCNGGA"
                     /annotation_group="TCCNGGA"
                     /label="PfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6327..6332
                     /Recognition_pattern="CCGC^GG"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(6389..6394)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(9)"
     misc_feature    6389..6394
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar"
     misc_feature    6416..6421
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI"
                     /note="Geneious type: restriction site"
     misc_feature    6425..6430
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    6433..6438
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI, Ple19I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(6438..6443)
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site(2)"
     misc_feature    6505..6510
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(2)"
     misc_feature    6548..6554
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     misc_feature    6553..6558
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    6553..6558
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    6582..6587
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    6607..6621
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="cPPT(1)"
     misc_feature    complement(6672..6677)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(10)"
     misc_feature    6672..6677
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(1)"
     misc_feature    6766..6771
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-SpeI scar(1)"
     misc_feature    6944..7124
                     /ApEinfo_label="HIV-1_5_LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIV-1_5_LTR(1)"
     misc_feature    6945..6950
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site(1)"
     misc_feature    complement(7077..7082)
                     /ApEinfo_label="XbaI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="XbaI-SpeI scar(1)"
     misc_feature    7082..7087
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(5)"
     misc_feature    complement(7082..7087)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(10)"
     misc_feature    complement(7118..7123)
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(5)"
     rep_origin      complement(7168..7850)
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1 origin"
     misc_feature    complement(7171..7790)
                     /ApEinfo_fwdcolor="#95928e"
                     /ApEinfo_revcolor="#95928e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="pBR322_origin"
     misc_feature    7227..7238
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /annotation_group="GACNNNNNNGTC"
                     /label="DrdI, AasI, DseDI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(7619..7624)
                     /ApEinfo_label="XbaI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="XbaI-SpeI scar(2)"
     misc_feature    complement(7766..7771)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(11)"
     misc_feature    7766..7771
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(2)"
     misc_feature    7777..7782
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(6)"
     misc_feature    complement(7777..7782)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(11)"
     misc_feature    7845..7850
                     /Recognition_pattern="T^CATGA"
                     /annotation_group="TCATGA"
                     /label="BspHI, CciI, PagI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(7863..7868)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(12)"
     misc_feature    7863..7868
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(3)"
     misc_feature    7875..7880
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(7)"
     misc_feature    complement(7875..7880)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(12)"
     CDS             complement(7948..8607)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="AmpR"
     misc_feature    8013..8023
                     /Recognition_pattern="GACNNN^NNGTC"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8085..8090)
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsaI site(3)"
     misc_feature    8207..8212
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="NheI-XbaI scar(3)"
     misc_feature    8238..8243
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    8243..8248
                     /Recognition_pattern="AA^CGTT"
                     /annotation_group="AACGTT"
                     /label="AclI, Psp1406I"
                     /note="Geneious type: restriction site"
     misc_feature    8385..8390
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI, Ple19I"
                     /note="Geneious type: restriction site"
     misc_feature    8496..8501
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    8534..8545
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)"
                     /annotation_group="CGANNNNNNTGC"
                     /label="BcgI"
                     /note="Geneious type: restriction site"
     misc_feature    8616..8621
                     /Recognition_pattern="AA^CGTT"
                     /annotation_group="AACGTT"
                     /label="AclI, Psp1406I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8643..8648)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(13)"
     misc_feature    8643..8648
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(4)"
     misc_feature    8660..8665
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BglII-BamHI scar(8)"
     misc_feature    complement(8660..8665)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BamHI-BglII scar(13)"
     misc_feature    8820..8825
                     /Recognition_pattern="AAT^ATT"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8847..8875)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="AmpR promoter"
     misc_feature    8853..8858
                     /Recognition_pattern="T^CATGA"
                     /annotation_group="TCATGA"
                     /label="BspHI, CciI, PagI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8857..8862)
                     /Recognition_pattern="CCGCTC(-3/-3)"
                     /annotation_group="CCGCTC"
                     /label="BsrBI, AccBSI, MbiI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttggtacag cagaagcctt tagaagttta agagctatgc tggaaacagc atagcaagtt
     3001 taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cgggagctaa
     3061 ggagtttata tggaaaccct tagcctgctg cgtaaggagt ttatatggaa acccttacgc
     3121 agcagttccc tttttttgtt ttttatgtct ctgcagagtt cggtctcgag tactaggatc
     3181 cattaggcgg ccgcgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
     3241 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     3301 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     3361 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     3421 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     3481 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     3541 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     3601 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3661 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3721 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3781 agcagagctg gtttagtgaa ccgtcagatc cgctagccca ccatgcccaa aaagaaaaga
     3841 aaagtgggta gtatgtccaa aaccatcgtt ctttcggtcg gcgaggctac tcgcactctg
     3901 actgagatcc agtccaccgc agaccgtcag atcttcgaag agaaggtcgg gcctctggtg
     3961 ggtcggctgc gcctcacggc ttcgctccgt caaaacggag ccaagaccgc gtatcgcgtc
     4021 aacctaaaac tggatcaggc ggacgtcgtt gattccggac ttccgaaagt gcgctacact
     4081 caggtatggt cgcacgacgt gacaatcgtt gcgaatagca ccgaggcctc gcgcaaatcg
     4141 ttgtacgatt tgaccaagtc cctcgtcgcg acctcgcagg tcgaagatct tgtcgtcaac
     4201 cttgtgccgc tgggccgtgg ctccggctct ggttcttccg acgaagaaga agcgcgcgag
     4261 ctgatcgagc gtgcgaagga ggctgcggaa cgcgcgcaag aagctgctga gcgtaccggt
     4321 gatccgcgcg tgcgtgaact cgctcgtgag ctgaaacgcc tggctcagga agctgctgaa
     4381 gaagtgaagc gtgacccgtc ttcctccgat gtgaacgaag cgctcaaact catcgtggaa
     4441 gctatcgagg cggctgtgga cgcgctcgaa gcggctgaac gcaccggcga cccggaggtt
     4501 cgtgagctgg cgcgtgagct cgtgcgcctc gcggttgagg ctgcggagga ggtgcaacgt
     4561 aacccatcct cctctgacgt gaatgaggct ctccacagca tagtttacgc tattgaagct
     4621 gccatttttg cattggaagc tgccgaaagg actggtgacc cggaagtgag agaactcgcc
     4681 cgagagcttg taagactggc ggttgaggcg gctgaagaag tccagcgaaa tccgtccagc
     4741 cggaatgtcg agcacgcact gatgaggatt gtgttggcca tttacctggc agaagaaaat
     4801 ctccgcgaag ctgaggagtc tggcgatccg gaaaaacgtg aaaaggctcg cgaacgtgtt
     4861 cgtgaggcgg tggaacgtgc ggaagaggtt cagcgtgatc catccggttg gctgaatcat
     4921 gaacaaaaac ttatttccga agaggatctg ggaagcggag ctactaactt cagcctgctg
     4981 aagcaggctg gagacgtgga ggagaaccct ggacctagcg agctgattaa ggagaacatg
     5041 cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag
     5101 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc
     5161 cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc
     5221 atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca
     5281 tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc
     5341 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac
     5401 ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac gctgtacccc
     5461 gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat
     5521 ctgatcgcaa acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg
     5581 cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa caacgagacc
     5641 tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg
     5701 cacaagctta attgataaga attcgtcgag ggacctaata acttcgtata gcatacatta
     5761 tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga tatcaagctt
     5821 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5881 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5941 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6001 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6061 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6121 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6181 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6241 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6301 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6361 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     6421 taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6481 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     6541 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6601 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     6661 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     6721 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6781 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     6841 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     6901 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     6961 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7021 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7081 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg
     7141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7261 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7381 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7501 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7621 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
