LOCUS       GWF297                  8453 bp ds-DNA     circular     15-SEP-2019
DEFINITION  TRE3G_2xMS2_wtf6_mcp-ANR-ANR-P2a-BFP.
ACCESSION   urn.local...s-aykyd2m
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     New DNA(6) from 1 to 8308 New DNA(5) from 1 to 8308 New DNA(4) from
            1 to 8308 New DNA(3) from 1 to 8308 New DNA(2) from 1 to 8308 New
            DNA(1) from 1 to 8308 U6_sgRNA_template from 1 to 8308 pMig-w from
            1 to 6471 ApEinfo:methylated:1.
FEATURES             Location/Qualifiers
     misc_feature    173..178
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    218..223
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /label="NruI, Bsp68I, BtuMI, RruI"
                     /note="Geneious type: restriction site"
     misc_feature    240..245
                     /Recognition_pattern="A^CGCGT"
                     /annotation_group="ACGCGT"
                     /label="MluI"
                     /note="Geneious type: restriction site"
     misc_feature    261..266
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    263..826
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pU6 (pMJ177) 5' CMV promoter IE"
     misc_feature    495..500
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    600..605
                     /Recognition_pattern="TAC^GTA"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    650..661
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     misc_feature    844..1024
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="HIV-1_5_LTR"
     misc_feature    845..850
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BsaI site"
     misc_feature    complement(977..982)
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="XbaI-SpeI scar"
     misc_feature    982..987
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar"
     misc_feature    complement(982..987)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(5)"
     misc_feature    complement(1018..1023)
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-XbaI scar(4)"
     misc_feature    1101..1106
                     /Recognition_pattern="G^CGCGC"
                     /annotation_group="GCGCGC"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1155..1160)
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-SpeI scar(2)"
     misc_feature    1165..1170
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-XbaI scar"
     misc_feature    1221..1226
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /label="NruI, Bsp68I, BtuMI, RruI"
                     /note="Geneious type: restriction site"
     misc_feature    1301..1306
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-XbaI scar(1)"
     misc_feature    complement(1497..1503)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    1535..1542
                     /Recognition_pattern="GGCCGG^CC"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    1558..1568
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    1581..1586
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    1695..1928
                     /ApEinfo_fwdcolor="#9cffe3"
                     /ApEinfo_revcolor="#9cffe3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="RRE"
     misc_feature    1984..1989
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-SpeI scar"
     misc_feature    2325..2331
                     /Recognition_pattern="RG^GWCCY"
                     /annotation_group="RGGWCCY"
                     /label="PpuMI, Psp5II, PspPPI"
                     /note="Geneious type: restriction site"
     misc_feature    2325..2331
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2380..2385
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(1)"
     misc_feature    complement(2380..2385)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(6)"
     misc_feature    2459..2473
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="cPPT"
     misc_feature    2596..2601
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(2)"
     misc_feature    complement(2596..2601)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(7)"
     misc_feature    2617..2622
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2617..2622
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    2625..2630
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    2630..2635
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(3)"
     misc_feature    complement(2630..2635)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(8)"
     misc_feature    2631..2933
                     /ApEinfo_fwdcolor="#fff81f"
                     /ApEinfo_revcolor="#fff81f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="U6 promoter"
     misc_feature    2725..2730
                     /Recognition_pattern="AAT^ATT"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2790..2795)
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-SpeI scar(3)"
     misc_recomb     2825..2858
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LoxP recombination site"
     misc_feature    2855..2860
                     /Recognition_pattern="TTA^TAA"
                     /annotation_group="TTATAA"
                     /label="PsiI, AanI"
                     /note="Geneious type: restriction site"
     motif           2945
                     /created_by="User"
                     /label="BstXI"
     misc_feature    2945..2965
                     /ApEinfo_fwdcolor="#800080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="sgTet3g"
     misc_feature    2946..2965
                     /Original_Bases="CAGACGCGAGGAAGGAGGGCGC"
                     /label="CAGACGCGAGGAAGGAGGGCGC"
                     /note="Geneious type: Editing History Replacement"
     misc_RNA        2966..3051
                     /created_by="User"
                     /label="modified_stem_loop"
     misc_RNA        3058..3076
                     /created_by="User"
                     /label="MS2"
     misc_feature    3079..3102
                     /Original_Bases="CACGAGCGACATGAGGATCACCCATGTCGCTCGTG"
                     /label="CACGAGCGACATGAGGATCACCCATGTCGCTCGTG"
                     /note="Geneious type: Editing History Replacement"
     misc_RNA        3084..3097
                     /created_by="User"
                     /label="f6"
     misc_RNA        3108..3127
                     /created_by="User"
                     /label="terminator"
     misc_feature    3128..3133
                     /ApEinfo_fwdcolor="#7bd5ff"
                     /ApEinfo_revcolor="#7bd5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="PstI"
     misc_feature    3139..3141
                     /ApEinfo_fwdcolor="#7bd5ff"
                     /ApEinfo_revcolor="#7bd5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Acc65I"
     misc_feature    3142..3147
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
                     /note="Geneious type: restriction site"
     misc_feature    3146..3151
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    3164..3171
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    3199..3781
                     /ApEinfo_fwdcolor="#ffed31"
                     /ApEinfo_revcolor="#ffed31"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="CMV promoter IE"
     misc_feature    3199..3762
                     /ApEinfo_label="pU6 (pMJ177) 5' CMV promoter IE"
                     /ApEinfo_fwdcolor="#fffc15"
                     /ApEinfo_revcolor="#fffc15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pU6 (pMJ177) 5' CMV promoter IE(1)"
     misc_feature    3431..3436
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    3536..3541
                     /Recognition_pattern="TAC^GTA"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    3586..3597
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     primer_bind     3764..3788
                     /Sequence="GCTGGTTTAGTGAACCGTCAGATCC"
                     /Hairpin_Tm="57.3"
                     /Tm="63.6"
                     /Self_Dimer_Tm="5.7"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="SecondInsert_seq"
     misc_feature    3783..3788
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(4)"
     misc_feature    complement(3783..3788)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(9)"
     misc_feature    3789..3794
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI, AsuNHI"
                     /note="Geneious type: restriction site"
     misc_feature    3789..3794
                     /Recognition_pattern="GCTAG^C"
                     /annotation_group="GCTAGC"
                     /label="BmtI, BspOI"
                     /note="Geneious type: restriction site"
     misc_binding    3803..3823
                     /ApEinfo_fwdcolor="#ff7e07"
                     /ApEinfo_revcolor="#08b0ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Nuclear Localization Signal (from SV40)"
     misc_feature    3830..4180
                     /gene="mcp1"
                     /SECDrawAs="Region"
                     /ApEinfo_fwdcolor="#ecff24"
                     /ApEinfo_revcolor="#f06bff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="MCPdelFG"
     misc_feature    3988..3993
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    4084..4089
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    4190..5230
                     /Original_Bases="CGGGCTGACGCATTGGACGATTTTGATCTGGATATGCTGGG
                     AAGTGACGCCCTCGATGATTTTGACCTTGACATGCTTGGTTCGGATGCCCTTGATGAC
                     TTTGACCTCGACATGCTCGGCAGTGACGCCCTTGATGATTTCGACCTGGACATGCTGA
                     TTAACTCTAGAAGTTCCGGATCTCCGAAAAAGAAACGCAAAGTTGGTAGCCAGTACCT
                     GCCCGACACCGACGACCGGCACCGGATCGAGGAAAAGCGGAAGCGGACCTACGAGACA
                     TTCAAGAGCATCATGAAGAAGTCCCCCTTCAGCGGCCCCACCGACCCTAGACCTCCAC
                     CTAGAAGAATCGCCGTGCCCAGCAGATCCAGCGCCAGCGTGCCAAAACCTGCCCCCCA
                     GCCTTACCCCTTCACCAGCAGCCTGAGCACCATCAACTACGACGAGTTCCCTACCATG
                     GTGTTCCCCAGCGGCCAGATCTCTCAGGCCTCTGCTCTGGCTCCAGCCCCTCCTCAGG
                     TGCTGCCTCAGGCTCCTGCTCCTGCACCAGCTCCAGCCATGGTGTCTGCACTGGCTCA
                     GGCACCAGCACCCGTGCCTGTGCTGGCTCCTGGACCTCCACAGGCTGTGGCTCCACCA
                     GCCCCTAAACCTACACAGGCCGGCGAGGGCACACTGTCTGAAGCTCTGCTGCAGCTGC
                     AGTTCGACGACGAGGATCTGGGAGCCCTGCTGGGAAACAGCACCGATCCTGCCGTGTT
                     CACCGACCTGGCCAGCGTGGACAACAGCGAGTTCCAGCAGCTGCTGAACCAGGGCATC
                     CCTGTGGCCCCTCACACCACCGAGCCCGTGCTGATGGAATACCCCGAGGCCATCACCC
                     GGCTCGTGACAGGCGCTCAGAGGCCTCCTGATCCAGCTCCTGCCCCTCTGGGAGCACC
                     AGGCCTGCCTAATGGACTGCTGTCTGGCGACGAGGACTTCAGCTCTATCGCCGATATG
                     GATTTCTCAGCCTTGCTGGGCTCTGGCAGCGGCAGCCGGGATTCCAGGGAAGGGATGT
                     TTTTGCCGAAGCCTGAGGCCGGCTCCGCTATTAGTGACGTGTTTGAGGGCCGCGAGGT
                     GTGCCAGCCAAAACGAATCCGGCCATTTCATCCTCCAGGAAGTCCATGGGCCAACCGC
                     CCACTCCCCGCCAGCCTCGCACCAACACCAACCGGTCCAGTACATGAGCCAGTCGGGT
                     CACTGACCCCGGCACCAGTCCCTCAGCCACTGGATCCAGCGCCCGCAGTGACTCCCGA
                     GGCCAGTCACCTGTTGGAGGATCCCGATGAAGAGACGAGCCAGGCTGTCAAAGCCCTT
                     CGGGAGATGGCCGATACTGTGATTCCCCAGAAGGAAGAGGCTGCAATCTGTGGCCAAA
                     TGGACCTTTCCCATCCGCCCCCAAGGGGCCATCTGGATGAGCTGACAACCACACTTGA
                     GTCCATGACCGAGGATCTGAACCTGGACTCACCCCTGACCCCGGAATTGAACGAGATT
                     CTGGATACCTTCCTGAACGACGAGTGCCTCTTGCATGCCATGCATATCAGCACAGGAC
                     TGTCCATCTTCGACACATCTCTGTTTTGACCCGGGTCGAGCATGCATCTAGGGCGGCC
                     AATTCCGCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATA
                     AGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAAT
                     GTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCC
                     CTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGA
                     AGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCA
                     CCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGG
                     CGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCT
                     CTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATG
                     GGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAA
                     AACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATA
                     AATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTC
                     AAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCG
                     AGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCC
                     CCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTAC
                     GTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCA
                     AGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC
                     CTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCC
                     TCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGA
                     TGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGA
                     CGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAG
                     CTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACT
                     ACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGA
                     CGAGCTGTACAAG"
                     /label="CGGGCTGACGCATTGGACGATTTTGATCTGGATATGCTGGGAAGTGACGC
                     CCTCGATGATTTTGACCTTGACATGCTTGGTTCGGATGCCCTTGATGACTTTGACCTC
                     GACATGCTCGGCAGTGACGCCCTTGATGATTTCGACCTGGACATGCTGATTAACTCTA
                     GAAGTTCCGGATCTCCGAAAAAGAAACGCAAAGTTGGTAGCCAGTACCTGCCCGACAC
                     CGACGACCGGCACCGGATCGAGGAAAAGCGGAAGCGGACCTACGAGACATTCAAGAGC
                     ATCATGAAGAAGTCCCCCTTCAGCGGCCCCACCGACCCTAGACCTCCACCTAGAAGAA
                     TCGCCGTGCCCAGCAGATCCAGCGCCAGCGTGCCAAAACCTGCCCCCCAGCCTTACCC
                     CTTCACCAGCAGCCTGAGCACCATCAACTACGACGAGTTCCCTACCATGGTGTTCCCC
                     AGCGGCCAGATCTCTCAGGCCTCTGCTCTGGCTCCAGCCCCTCCTCAGGTGCTGCCTC
                     AGGCTCCTGCTCCTGCACCAGCTCCAGCCATGGTGTCTGCACTGGCTCAGGCACCAGC
                     ACCCGTGCCTGTGCTGGCTCCTGGACCTCCACAGGCTGTGGCTCCACCAGCCCCTAAA
                     CCTACACAGGCCGGCGAGGGCACACTGTCTGAAGCTCTGCTGCAGCTGCAGTTCGACG
                     ACGAGGATCTGGGAGCCCTGCTGGGAAACAGCACCGATCCTGCCGTGTTCACCGACCT
                     GGCCAGCGTGGACAACAGCGAGTTCCAGCAGCTGCTGAACCAGGGCATCCCTGTGGCC
                     CCTCACACCACCGAGCCCGTGCTGATGGAATACCCCGAGGCCATCACCCGGCTCGTGA
                     CAGGCGCTCAGAGGCCTCCTGATCCAGCTCCTGCCCCTCTGGGAGCACCAGGCCTGCC
                     TAATGGACTGCTGTCTGGCGACGAGGACTTCAGCTCTATCGCCGATATGGATTTCTCA
                     GCCTTGCTGGGCTCTGGCAGCGGCAGCCGGGATTCCAGGGAAGGGATGTTTTTGCCGA
                     AGCCTGAGGCCGGCTCCGCTATTAGTGACGTGTTTGAGGGCCGCGAGGTGTGCCAGCC
                     AAAACGAATCCGGCCATTTCATCCTCCAGGAAGTCCATGGGCCAACCGCCCACTCCCC
                     GCCAGCCTCGCACCAACACCAACCGGTCCAGTACATGAGCCAGTCGGGTCACTGACCC
                     CGGCACCAGTCCCTCAGCCACTGGATCCAGCGCCCGCAGTGACTCCCGAGGCCAGTCA
                     CCTGTTGGAGGATCCCGATGAAGAGACGAGCCAGGCTGTCAAAGCCCTTCGGGAGATG
                     GCCGATACTGTGATTCCCCAGAAGGAAGAGGCTGCAATCTGTGGCCAAATGGACCTTT
                     CCCATCCGCCCCCAAGGGGCCATCTGGATGAGCTGACAACCACACTTGAGTCCATGAC
                     CGAGGATCTGAACCTGGACTCACCCCTGACCCCGGAATTGAACGAGATTCTGGATACC
                     TTCCTGAACGACGAGTGCCTCTTGCATGCCATGCATATCAGCACAGGACTGTCCATCT
                     TCGACACATCTCTGTTTTGACCCGGGTCGAGCATGCATCTAGGGCGGCCAATTCCGCC
                     CCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTG
                     TGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCC
                     CGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCA
                     AAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTG
                     AAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGAC
                     AGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAAC
                     CCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTCAAG
                     CGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGAT
                     CTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAG
                     GCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAAATGGTGAG
                     CAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCAC
                     ATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCC
                     CCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTT
                     CGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCAC
                     CCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGC
                     GCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCA
                     GGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGC
                     CCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCG
                     AGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCA
                     CTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGC
                     GCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCG
                     TGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTA
                     CAAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4190..4264
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Linker"
     misc_feature    4265..4327
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ANR"
     misc_feature    4328..4402
                     /ApEinfo_label="Linker"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Linker(1)"
     misc_feature    4403..4465
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ANR"
     gene            <4466..>4531
                     /created_by="User"
                     /modified_by="User"
                     /label="P2a"
     gene            <4532..5227
                     /created_by="User"
                     /label="BFP"
     misc_feature    5234..5239
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    5245..5251
                     /Recognition_pattern="RG^GWCCY"
                     /annotation_group="RGGWCCY"
                     /label="PpuMI, Psp5II, PspPPI"
                     /note="Geneious type: restriction site"
     misc_recomb     5253..5286
                     /ApEinfo_label="LoxP recombination site"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="LoxP recombination site(1)"
     primer_bind     complement(5279..5305)
                     /Sequence="CCGGAACCCTTAAACATGTATAACTTC"
                     /Hairpin_Tm="None"
                     /Tm="60.2"
                     /Self_Dimer_Tm="None"
                     /%GC="40.7"
                     /created_by="primer3"
                     /label="SecondInsert_rev_seq"
     misc_feature    5287..5292
                     /Recognition_pattern="A^CATGT"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     misc_feature    5332..5932
                     /ApEinfo_fwdcolor="#0060ff"
                     /ApEinfo_revcolor="#0060ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="wPRE"
     misc_feature    5336..5341
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI"
                     /note="Geneious type: restriction site"
     misc_feature    5842..5847
                     /Recognition_pattern="CCGC^GG"
                     /annotation_group="CCGCGG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5904..5909)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(9)"
     misc_feature    5904..5909
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar"
     misc_feature    5931..5936
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI"
                     /note="Geneious type: restriction site"
     misc_feature    5948..5953
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI, Ple19I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5953..5958)
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BsaI site(2)"
     misc_feature    6020..6025
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-XbaI scar(2)"
     misc_feature    6068..6073
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    6068..6073
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    6122..6136
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="cPPT(1)"
     misc_feature    complement(6187..6192)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(10)"
     misc_feature    6187..6192
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(1)"
     misc_feature    6281..6286
                     /ApEinfo_label="NheI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-SpeI scar(1)"
     misc_feature    6459..6639
                     /ApEinfo_label="HIV-1_5_LTR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="HIV-1_5_LTR(1)"
     misc_feature    6460..6465
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BsaI site(1)"
     misc_feature    complement(6592..6597)
                     /ApEinfo_label="XbaI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="XbaI-SpeI scar(1)"
     misc_feature    6597..6602
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(5)"
     misc_feature    complement(6597..6602)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(10)"
     misc_feature    complement(6633..6638)
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-XbaI scar(5)"
     rep_origin      complement(6683..7365)
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ColE1 origin"
     misc_feature    complement(6686..7305)
                     /ApEinfo_fwdcolor="#95928e"
                     /ApEinfo_revcolor="#95928e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pBR322_origin"
     misc_feature    complement(7134..7139)
                     /ApEinfo_label="XbaI-SpeI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="XbaI-SpeI scar(2)"
     misc_feature    complement(7281..7286)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(11)"
     misc_feature    7281..7286
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(2)"
     misc_feature    7292..7297
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(6)"
     misc_feature    complement(7292..7297)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(11)"
     misc_feature    complement(7378..7383)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(12)"
     misc_feature    7378..7383
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(3)"
     misc_feature    7390..7395
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(7)"
     misc_feature    complement(7390..7395)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(12)"
     CDS             complement(7463..8122)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     misc_feature    7528..7538
                     /Recognition_pattern="GACNNN^NNGTC"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(7600..7605)
                     /ApEinfo_label="BsaI site"
                     /ApEinfo_fwdcolor="#b81bff"
                     /ApEinfo_revcolor="#ff8dff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BsaI site(3)"
     misc_feature    7722..7727
                     /ApEinfo_label="NheI-XbaI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="NheI-XbaI scar(3)"
     misc_feature    7753..7758
                     /Recognition_pattern="TGC^GCA"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    7900..7905
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI, Ple19I"
                     /note="Geneious type: restriction site"
     misc_feature    8011..8016
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    8049..8060
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)"
                     /annotation_group="CGANNNNNNTGC"
                     /label="BcgI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8158..8163)
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(13)"
     misc_feature    8158..8163
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(4)"
     misc_feature    8175..8180
                     /ApEinfo_label="BglII-BamHI scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BglII-BamHI scar(8)"
     misc_feature    complement(8175..8180)
                     /ApEinfo_label="BamHI-BglII scar"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BamHI-BglII scar(13)"
     misc_feature    8335..8340
                     /Recognition_pattern="AAT^ATT"
                     /annotation_group="AATATT"
                     /label="SspI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8362..8390)
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR promoter"
ORIGIN      
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt
     2941 gttggtacgt tctctatcac tgatagttta agagctatgc tggaaacagc atagcaagtt
     3001 taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cgggagcaca
     3061 tgaggatcac ccatgtgcga ctcccacagt cactggggag tcttcccttt ttttgttttt
     3121 tatgtctctg cagagttcgg tctcgagtac taggatccat taggcggccg cgtggataac
     3181 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     3241 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3301 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3361 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3421 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3481 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3541 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3601 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     3661 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     3721 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     3781 tcagatccgc tagcccacca tgcccaaaaa gaaaagaaaa gtgggtagta tggcttctaa
     3841 ctttactcag ttcgttctcg tcgacaatgg cggaactggc gacgtgactg tcgccccaag
     3901 caacttcgct aacgggatcg ctgaatggat cagctctaac tcgcgttcac aggcttacaa
     3961 agtaacctgt agcgttcgtc agagctctgc gcagaatcgc aaatacacca tcaaagtcga
     4021 ggtgcctaaa ggcgcctggc gttcgtactt aaatatggaa ctaaccattc caattttcgc
     4081 cacgaattcc gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa
     4141 cccgattccc tcagcaatcg cagcaaactc cggcatctac ggctccggag gttccgggac
     4201 gggatctggt actggttctg gtaccgggac aacgtccgga acagggacag ggggctccac
     4261 tgggggtgaa ttggacgagt tggtttatct gctcgacggg cctggttatg atcccatcca
     4321 ttccgatgga agcggtactg gtagcggcac aggctccgga acgggcacga cgagcggtac
     4381 aggaacaggt ggtagtacag gtggggagct tgatgagctt gtctacctcc ttgacggccc
     4441 cggttacgac cctatccata gcgacggaag cggagctact aacttcagcc tgctgaagca
     4501 ggctggagac gtggaggaga accctggacc tagcgagctg attaaggaga acatgcacat
     4561 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     4621 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     4681 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     4741 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     4801 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     4861 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     4921 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     4981 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     5041 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     5101 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     5161 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     5221 gcttaattga taagaattcg tcgagggacc taataacttc gtatagcata cattatacga
     5281 agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga
     5341 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5401 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5461 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5521 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5581 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5641 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5701 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5761 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5821 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5881 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     5941 tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6001 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6061 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6121 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6181 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     6241 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     6301 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     6361 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     6421 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     6481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc
     6661 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6721 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6781 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6841 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6901 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6961 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7021 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7081 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7141 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7201 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7261 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7321 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7381 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7441 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7501 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7561 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7621 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7681 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7741 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7801 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7861 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7921 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7981 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8041 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8101 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8161 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8221 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8281 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8341 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8401 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
