LOCUS       Exported                6076 bp ds-DNA     circular SYN 17-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 17, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     LOCUS pET-28 a (+) 5369 bp DNA SYN 17-Apr-2014 DEFINITION pET-28 a 
            (+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial 
            sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(1..129)
                     /label=Untitled
     primer_bind     complement(164..222)
                     /label=50_BsMTS-pET
     primer_bind     164..173
                     /label=50_BsMTS-pET
     misc_feature    complement(179..226)
                     /label=MTS
                     /note="MTS"
     misc_feature    complement(182..226)
                     /label=Bs_MTS
                     /note="Bs_MTS"
     primer_bind     202..256
                     /label=61_BsMTS_mCh_rev
     misc_feature    complement(227..235)
                     /label=GSG
                     /note="GSG"
     misc_feature    complement(236..943)
                     /label=mCherry
                     /note="mCherry"
     primer_bind     complement(926..961)
                     /label=19_mCherry_fw
     misc_feature    complement(944..976)
                     /label=Linker (from pDS277)
                     /note="Linker (from pDS277)"
     primer_bind     944..962
                     /label=20_pET28a_rev
     misc_feature    977..994
                     /label=His-Tag
                     /note="His-Tag"
     promoter        complement(1077..1093)
                     /label=T7-Promotor
                     /note="T7-Promotor"
     gene            complement(1125..1388)
                     /label=tet
                     /note="tet(564-300)"
     misc_feature    1471..2562
                     /label=lacI
                     /note="lacI"
     gene            complement(2621..2896)
                     /label=tet
                     /note="tet(852-576)"
     misc_feature    3371..3562
                     /label=ROP
                     /note="ROP"
     rep_origin      complement(3977..4596)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="pBR322_origin"
     gene            4702..5517
                     /label=KanR2
                     /note="KanR2"
     rep_origin      complement(5742..6048)
                     /direction=LEFT
                     /label=f1_origin
                     /note="f1_origin"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttt
      181 aagaacgaac accgaagaaa gatttgattt tagccatcat acctttgcca gatcccttgt
      241 acagctcgtc catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga
      301 tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg
      361 gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc
      421 cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca
      481 tccgctcgga ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg
      541 ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt
      601 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
      661 cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
      721 tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct
      781 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct
      841 cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact
      901 ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catcctagga cttccacctg
      961 aacctccaga tccaccgtga tgatgatgat gatggctgct gcccatggta tatctccttc
     1021 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
     1081 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
     1141 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1201 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1261 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1321 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1381 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca
     1441 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
     1501 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
     1561 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
     1621 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
     1681 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
     1741 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
     1801 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
     1861 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
     1921 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
     1981 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
     2041 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
     2101 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
     2161 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
     2221 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
     2281 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
     2341 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     2401 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     2461 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2521 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2581 aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc
     2641 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     2701 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     2761 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     2821 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     2881 cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     2941 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     3001 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     3061 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
     3121 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
     3181 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
     3241 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     3301 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac
     3361 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
     3421 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat
     3481 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg
     3541 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     3601 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3661 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     3721 gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata ccgcacagat
     3781 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     3841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3901 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3961 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4021 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4081 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4141 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4201 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4261 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4321 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4381 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     4441 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4501 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4621 ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa taaaactgtc tgcttacata
     4681 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctctaggccg
     4741 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     4801 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
     4861 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     4921 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     4981 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
     5041 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     5101 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     5161 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     5221 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     5281 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     5341 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     5401 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     5461 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaagaa
     5521 ttaattcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5581 gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt
     5641 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     5701 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     5761 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5821 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5881 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5941 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     6001 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     6061 cgcgtcccat tcgcca
//
