LOCUS       Exported                4490 bp ds-DNA     circular SYN 18-SEP-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Maximilian Heumuller
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 18, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          673..678
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        249..607
                     /label=Camk2a
                     /note="color: #00ccff; direction: RIGHT"
     promoter        608..625
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    639..678
                     /label=MCS
                     /note="color: #ffff00"
     CDS             679..1425
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    1441..1443
                     /label=Belongs to bGH
                     /note="color: #a6acb3"
     polyA_signal    1444..1668
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     repeat_region   1753..1893
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      1968..2423
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        2705..2809
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2810..3670
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2810 .. 2878 / #ccffcc / signal sequence
                        2: 2879 .. 3670 / #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3818..4485
                     /direction=RIGHT
                     /label=pUCori
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccaat tcagtcgata actataacgg tcctaaggta
      181 gcgatttaaa tacgcgctct cttaaggtag ccccgggacg cgtcaattgc atgaagaatc
      241 tgcttaggat gtggactaag tttgttcaca tccccttctc caaccccctc agtacatcac
      301 cctgggggaa cagggtccac ttgctcctgg gcccacacag tcctgcagta ttgtgtatat
      361 aaggccaggg caaagaggag caggttttaa agtgaaaggc aggcaggtgt tggggaggca
      421 gttaccgggg caacgggaac agggcgtttc ggaggtggtt gccatgggga cctggatgct
      481 gacgaaggct cgcgaggctg tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt
      541 cagtcaagcc ggttctccgt ttgcactcag gagcacgggc aggcgagtgg cccctagttc
      601 tgggagtaat acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt
      661 ggtaccgagc tcggatccat gtctagactg gacaagagca aagtcataaa ctctgctctg
      721 gaattactca atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
      781 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
      841 ctgccaatcg agatgctgga caggcatcat acccactcct gccccctgga aggcgagtca
      901 tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc
      961 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
     1021 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
     1081 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
     1141 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgaa acaagcaatt
     1201 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
     1261 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat
     1321 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     1381 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaacccgc tgatcagcct
     1441 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1501 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1561 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1621 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     1681 aaagaaccag atcctctctt aaggtagcat cgagatttaa attagggata acagggtaat
     1741 ggcgcgggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     1801 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     1861 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     1921 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     1981 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2041 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2101 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2161 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     2221 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2281 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     2341 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2401 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     2461 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2521 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2581 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     2641 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3781 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3961 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
