LOCUS Exported 5185 bp ds-DNA circular SYN 18-SEP-2019 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5185) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Sep 18, 2019 from SnapGene 4.3.11 https://www.snapgene.com FEATURES Location/Qualifiers source 1..5185 /organism="synthetic DNA construct" /mol_type="other DNA" /note="color: #ffffff" source 990..2118 /organism="synthetic DNA construct" /mol_type="other DNA" /note="color: #ffffff" source 1225..1233 /organism="synthetic DNA construct" /mol_type="other DNA" /note="color: #ffffff" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" /note="color: #ffe4c4" CDS 272..926 /label=CMV /note="color: #993366; direction: RIGHT" promoter 926..944 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff; direction: RIGHT" rep_origin 958..989 /direction=RIGHT /label=MCS /note="color: #ffff00" misc_feature 990..2118 /label=Fragment synthesized /note="color: #ff0000" misc_feature 990..1122 /label=FLEx /note="color: #ff6600" protein_bind complement(990..1023) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." /note="color: #31849b; direction: LEFT" protein_bind complement(1086..1119) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /note="color: #31849b; direction: LEFT" CDS complement(1135..1161) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /note="color: #cc99b2" /translation="YPYDVPDYA" CDS complement(1168..1194) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /note="color: #cc99b2" /translation="YPYDVPDYA" CDS complement(1198..1224) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /note="color: #cc99b2" /translation="YPYDVPDYA" CDS complement(1234..1977) /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /label=Tet-On(R) 3G /note="color: #993366" /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" misc_feature 1978..2118 /label=FLEx /note="color: #ff6600" protein_bind 1981..2014 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites." /note="color: #31849b; direction: RIGHT" protein_bind 2085..2118 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /note="color: #31849b; direction: RIGHT" misc_feature 2136..2138 /label=Belongs to bGH /note="color: #a6acb3" polyA_signal 2139..2363 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" /note="color: #a6acb3" repeat_region 2448..2588 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" /note="color: #ffe4c4" rep_origin 2663..3118 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00" promoter 3400..3504 /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: RIGHT" CDS 3505..4365 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 3505 .. 3573 / #ccffcc / signal sequence 2: 3574 .. 4365 / #ccffcc Cleavage site after base 3573" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4513..5180 /direction=RIGHT /label=pUCori /note="color: #ffff00" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccaat tcagtcgata actataacgg tcctaaggta 181 gcgatttaaa tacgcgctct cttaaggtag ccccgggacg cgtcaattgc atgaagaatc 241 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 301 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 361 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 421 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 481 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 541 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 601 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 661 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 721 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 781 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 841 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac 901 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct 961 agcgtttaaa cttaagcttg gtaccgagca taacttcgta taaagtatcc tatacgaagt 1021 tatatcaaaa taggaagacc aatgcttcac catcgacccg aattgccaag catcaccatc 1081 gacccataac ttcgtataat gtatgctata cgaagttata cttcatctag aaccagcgta 1141 atctggaacg tcataaggat aggctcctgc atagtccggc acgtcatacg ggtagcccgc 1201 atagtcagga acatcgtatg ggtatccgct tcccccgggg agcatgtcaa ggtcaaaatc 1261 gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag 1321 catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg 1381 tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg 1441 ccggtcgaac agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt 1501 ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa 1561 gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag 1621 gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg 1681 cactttagcc ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg 1741 cagaaagtct tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag 1801 catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt 1861 aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac 1921 tccattgagt aattccagag cagagtttat gactttgctc ttgtccagtc tagacatagt 1981 ataacttcgt ataggatact ttatacgaag ttatcattgg gattcttcct attttgatcc 2041 aagcatcacc atcgaccctc tagtccagat ctcaccatcg acccataact tcgtatagca 2101 tacattatac gaagttataa cccgctgatc agcctcgact gtgccttcta gttgccagcc 2161 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 2221 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 2281 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 2341 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagatcct ctcttaaggt 2401 agcatcgaga tttaaattag ggataacagg gtaatggcgc gggccgcagg aacccctagt 2461 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa 2521 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg 2581 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 2641 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 2701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 2761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 2821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg 2881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 2941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 3001 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 3061 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta 3121 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 3181 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 3241 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 3301 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 3361 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 3421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 3481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 3541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 3601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 3661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 3721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 3781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 3841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 3901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 3961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 4021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 4081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 4141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 4201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 4261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 4321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 4381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 4441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 4501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 4561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 4621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 4681 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 4741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 4801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 4861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 4921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 4981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 5041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 5101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 5161 ttttgctggc cttttgctca catgt //