LOCUS       Exported                3961 bp ds-DNA     circular SYN 18-SEP-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Clontech
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 18, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     Sequencing of the insert can be found at Seq004
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        1..451
                     /label=TRE3G BI promoter
                     /note="3rd-generation Tet-responsive bidirectional promoter
                     that can be activated by binding of Tet-On 3G"
                     /note="color: #ffffff; direction: BOTH"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    261..279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     CDS             474..899
                     /codon_start=1
                     /product="First part of ProRIP till the internal 
                     inactivation region."
                     /label=alpha-Chain_gePSI
                     /note="This feature has 2 segments:
                        1: 474 .. 896 / #3366ff
                        2: 897 .. 899 / #3366ff"
                     /translation="MKFTEIFPVEDANYPYSAFIASVRKDVIKHCTDHKGIFQPVLPPE
                     KKVPELWLYTELKTRTSSITLAIRMDNLYLVGFRTPGGVWWEFGKDGDTHLLGDNPRWL
                     GFGGRYQDLIGNKGLETVTMGRAEMTRAVNDLAKKKK"
     polyA_signal    1031..1112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(1294..1882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(2053..2913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2053 .. 2844 / #ccffcc
                        2: 2845 .. 2913 / #ccffcc / signal sequence
                     Cleavage site after base 2844"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2914..3018)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    3051..3132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(3252..3947)
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /note="color: #05fd14"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
ORIGIN
        1 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac
       61 ctcgacatac tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
      121 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
      181 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
      241 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
      301 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
      361 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
      421 aactttccgt accacttcct accctcgtaa agtcgacacc ggggcccaga tctatgaagt
      481 tcactgaaat cttccccgtg gaggacgcga actaccctta cagcgccttc atcgcgtcgg
      541 tccggaaaga cgtgatcaaa cactgcaccg accataaagg gatcttccag cccgtgctgc
      601 caccggagaa gaaggtcccg gagctatggt tgtacacaga gctcaaaact aggaccagct
      661 ccatcacgct cgccatacgc atggacaacc tgtacctcgt gggcttcagg accccgggcg
      721 gggtgtggtg ggagttcggc aaggacggcg acacccacct cctcggcgac aaccccaggt
      781 ggctcggctt cggcggcagg taccaggacc tcatcggcaa caagggtctg gagaccgtca
      841 ccatgggccg cgccgaaatg accagggccg tcaacgacct ggcgaagaag aagaagtgag
      901 cggccgccgg cgatatctcc agaggatcat aatcagccat accacatttg tagaggtttt
      961 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1021 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1081 aaatttcaca aataaagcat ttttttcact gccccgagct tcctcgctca ctgactcgct
     1141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     1501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     1741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1921 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1981 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2161 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3001 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg gcagtgaaaa
     3061 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3121 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     3181 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     3241 ctagactgca gtcagggcaa ggcggagccg gaggcgatgg cgtgctcggt caggtgccac
     3301 ttctggttct tggcgtcgct gcggtcctcg cgggtcagct tgtgctggat gaagtgccag
     3361 tcgggcatct tgcggggcac ggacttggcc ttgtacacgg tgtcgaactg gcagcgcaag
     3421 cggccaccgt ccttcagcag caggtacatg ctcacgtcgc ccttcaagat gccctgcttg
     3481 ggcacgggga tgatcttctc gcaggagggc tcccagttgt cggtcatctt cttcatcacg
     3541 gggccgtcgg cggggaagtt cacgccgtag aacttggact cgtggtacat gcagttctcc
     3601 tccacgctca cggtgatgtc ggcgttgcag atgcacacgg cgccgtcctc gaacaggaag
     3661 gagcggtccc aggtgtagcc ggcggggcag gagttcttga agtagtcgac gatgtcctgg
     3721 gggtactcgg tgaacacgcg gttgccgtac atgaaggcgg cggacaagat gtcctcggcg
     3781 aagggcaagg ggccgccctc caccacgcac aggttgatgg cctgcttgcc cttgaagggg
     3841 tagccgatgc cctcgccggt gatcacgaac ttgtggccgt ccacgcagcc ctccatgcgg
     3901 tacttcatgg tcatctcctt ggtcaggccg tgcttggact gggccatggt gaattctcca
     3961 g
//
