LOCUS       Exported                3847 bp ds-DNA     circular SYN 18-SEP-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Clontech
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 18, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
COMMENT     Sequencing of the insert can be found at Seq004
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        1..451
                     /label=TRE3G BI promoter
                     /note="3rd-generation Tet-responsive bidirectional promoter
                     that can be activated by binding of Tet-On 3G"
                     /note="color: #ffffff; direction: BOTH"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    261..279
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     CDS             474..785
                     /codon_start=1
                     /product="Second part of ProRIP from behind the internal 
                     inactivation region"
                     /label=beta-Chain_gePSI
                     /note="This feature has 2 segments:
                        1: 474 .. 476 / #3366ff
                        2: 477 .. 785 / #3366ff"
                     /translation="MAADPQADTKSKLVKLVVMVCEGLRFNTVSRTVDAGFNSQHGVTL
                     TVTQGKQVQKWDRISKAAFEWADHPTAVIPDMQKLGIKDKNEAARIVALVKNQTTAAA"
     polyA_signal    917..998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(1180..1768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(1939..2799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1939 .. 2730 / #ccffcc
                        2: 2731 .. 2799 / #ccffcc / signal sequence
                     Cleavage site after base 2730"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2800..2904)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    2937..3018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(3138..3833)
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /note="color: #05fd14"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
ORIGIN
        1 gcgatctgac ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac
       61 ctcgacatac tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
      121 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
      181 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
      241 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
      301 tatcagtgat agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga
      361 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc
      421 aactttccgt accacttcct accctcgtaa agtcgacacc ggggcccaga tctatggcgg
      481 ctgacccaca ggccgacacg aagagcaagc tggtgaagct ggtggtcatg gtgtgcgagg
      541 ggctgcggtt caacaccgtg tcccgcacgg tggacgcggg gttcaacagc cagcacgggg
      601 tgaccttgac cgtgacgcag gggaagcagg tgcagaagtg ggacaggatc tccaaggcgg
      661 ccttcgagtg ggctgaccac cccaccgctg tgatccccga catgcagaag cttggcatca
      721 aggataagaa cgaagcagcg aggatcgttg cgctcgttaa gaatcaaact actgcggcgg
      781 cttgagcggc cgccggcgat atctccagag gatcataatc agccatacca catttgtaga
      841 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      901 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      961 catcacaaat ttcacaaata aagcattttt ttcactgccc cgagcttcct cgctcactga
     1021 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1081 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1141 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1201 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1261 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1321 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     1381 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1441 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1501 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1561 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1621 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1681 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1741 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1801 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1861 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1921 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1981 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2041 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2101 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2161 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2221 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2281 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2341 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2401 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2461 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2521 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2581 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2641 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2701 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2761 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2821 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2881 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag
     2941 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3001 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3061 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     3121 gatcctctag actgcagtca gggcaaggcg gagccggagg cgatggcgtg ctcggtcagg
     3181 tgccacttct ggttcttggc gtcgctgcgg tcctcgcggg tcagcttgtg ctggatgaag
     3241 tgccagtcgg gcatcttgcg gggcacggac ttggccttgt acacggtgtc gaactggcag
     3301 cgcaagcggc caccgtcctt cagcagcagg tacatgctca cgtcgccctt caagatgccc
     3361 tgcttgggca cggggatgat cttctcgcag gagggctccc agttgtcggt catcttcttc
     3421 atcacggggc cgtcggcggg gaagttcacg ccgtagaact tggactcgtg gtacatgcag
     3481 ttctcctcca cgctcacggt gatgtcggcg ttgcagatgc acacggcgcc gtcctcgaac
     3541 aggaaggagc ggtcccaggt gtagccggcg gggcaggagt tcttgaagta gtcgacgatg
     3601 tcctgggggt actcggtgaa cacgcggttg ccgtacatga aggcggcgga caagatgtcc
     3661 tcggcgaagg gcaaggggcc gccctccacc acgcacaggt tgatggcctg cttgcccttg
     3721 aaggggtagc cgatgccctc gccggtgatc acgaacttgt ggccgtccac gcagccctcc
     3781 atgcggtact tcatggtcat ctccttggtc aggccgtgct tggactgggc catggtgaat
     3841 tctccag
//
