LOCUS       Exported                9415 bp ds-DNA     circular SYN 03-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Tumor Cell Biology
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 18, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..513
                     /label=LTR
                     /note="MSCV left LTR, which is a hybrid of PCMV and a mouse
                     endogenous virus."
     repeat_region   1..370
                     /label=U3_PCMV
                     /note="U3 region from PCMV virus, which was published as 
                     modified by passaging to enable more efficient infection of
                     embryonal carcinoma cells. It has lost one of the 75 bp 
                     repeats, but other than that is nearly identical to MPSV 
                     xxwhen you take into account the errors in the genbank 
                     sequencexx."
     misc_feature    1..39
                     /label=LTR sequence found in Reverse PCR
                     /note="LTR sequence found in Reverse PCR"
     repeat_region   1..11
                     /label=MLV_LTR_tip
                     /note="Tip of both MLV and MPSV LTR."
     primer_bind     complement(289..313)
                     /label=SJVL-376_Max=494
                     /note="In MSCV U3, facing left. Max recorded sequencing 
                     read = 494 bases. Perfect match Tm = 60.6 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     AGAAGCGAGA AGCGAACTGA TTGGT. This primer matches this site 
                     completely."
     primer_bind     296..318
                     /label=SJVL-728_Max=
                     /note="mlv pbs primer Max recorded sequencing read = bases.
                     Perfect match Tm = 58.9 C, although for oligos less than 35
                     bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is AGTTCGCTTC
                     TCGCTTCTGT TCG. This primer matches this site perfectly."
     primer_bind     320..336
                     /label=SJVL-783_Max=
                     /note="MLV ECT3- U3 primer facing right. Max recorded 
                     sequencing read = bases. Perfect match Tm = 57.0 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is GCGCTTCTGC TCCCCGA. This primer matches this 
                     site perfectly."
     primer_bind     328..350
                     /label=SJVL-781_Max=
                     /note="MLV U3 facing right. Max recorded sequencing read = 
                     bases. Perfect match Tm = 57.4 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCTCCCCGAG
                     CTCAATAAAA GAG. This primer matches this site perfectly."
     primer_bind     346..366
                     /label=SJVL-780_Max=
                     /note="MLV U3, same as 725 but shifted left a little. Max 
                     recorded sequencing read = bases. Perfect match Tm = 56.3 
                     C, although for oligos less than 35 bp, only the 3 prime 17
                     nt must match to trigger annotation. The actual sequence of
                     the primer is AAGAGCCCAC AACCCCTCAC T. This primer matches 
                     this site perfectly."
     primer_bind     349..368
                     /label=SJVL-903_Max=
                     /note="Biotinylated oligo for MLV vector DNA purification. 
                     Max recorded sequencing read = bases. Perfect match Tm = 
                     58.5 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is AGCCCACAAC CCCTCACTCG . This 
                     primer matches this site perfectly."
     primer_bind     350..368
                     /label=SJVL-725_Max=
                     /note="MLV PBS pcr Max recorded sequencing read = bases. 
                     Perfect match Tm = 57.4 C, although for oligos less than 35
                     bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCCCACAACC
                     CCTCACTCG. This primer matches this site perfectly."
     primer_bind     355..384
                     /label=SJVL-740_Max=
                     /note="MLV U3-R junction mutagenesis plasmid. Creates Asc I
                     site. Max recorded sequencing read = bases. Perfect match 
                     Tm = 80.2 C, although for oligos less than 35 bp, only the 
                     3 prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is CAACCCCTCA CTCGGCGCGC CAGTCCTCCG 
                     . This primer matches this site perfectly."
     repeat_region   371..439
                     /label=R_MoPBSQ
                     /note="R region from MFG-SxxMPSVxxPBSQxx. After the Kpn I 
                     site, it is an endogenous murine virus. Before that, it is 
                     MPSV."
     repeat_region   440..513
                     /label=U5_PBSQ
                     /note="U5 from MFG-SxxMPSV-PBSQxx. Derives from an 
                     endogenous murine virus which contained the PBS-Q."
     repeat_region   complement(503..513)
                     /label=MLV_LTR_tip
                     /note="Tip of both MLV and MPSV LTR."
     primer_bind     complement(516..533)
                     /label=PBS-Q
                     /note="tRNA binding site for MMP vectors, for Glutamine 
                     tRNA."
     misc_feature    534..1403
                     /label=Psi_MSCV
                     /note="Everything from PBS-Q to end of MLV sequence at Psi 
                     position in MSCV vectors. Everything left of Pst I site is 
                     from endogenous murine PBS-Q virus. After that, but for ATG
                     -> AG mutation in gag, it is identical to moloney MLV. This
                     version does not have an Avr II site, and is confirmed in 
                     the MSCV-neo construct."
     primer_bind     582..603
                     /label=SJVL-495_Max=
                     /note="MSCV Psi PCR left. Max recorded sequencing read = 
                     bases. Perfect match Tm = 64.9 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is CTGGCCAGCG
                     GTCGTTTCGT GT. This primer matches this site perfectly."
     primer_bind     complement(677..700)
                     /label=SJVL-711_Max=870
                     /note="MLV sequencing primer. In Psi facing left. Max 
                     recorded sequencing read = 870 bases. Perfect match Tm = 
                     66.3 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is CCACGGGTCC GCCAGATACA GAGC. This 
                     primer matches this site completely."
     primer_bind     complement(716..739)
                     /label=SJVL-496_Max=
                     /note="MSCV Psi PCR right. Max recorded sequencing read = 
                     bases. Perfect match Tm = 72 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is CCAGGGTTGC
                     GGCCGGGTGT TCAG. This primer matches this site completely."
     primer_bind     829..853
                     /label=SJVL-574_Max=
                     /note="MLV csgag real time primer left. May work on MSCV. 
                     Max recorded sequencing read = bases. Perfect match Tm = 
                     54.7 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is TTCTGGTAGG AGACGAGAAC CTAAA. This
                     primer matches this site perfectly."
     primer_bind     855..880
                     /label=SJVL-576_Max=
                     /note="MLV real time probe. May work on MSCV. Max recorded 
                     sequencing read = bases. Perfect match Tm = 64.9 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is CAGTTCCCGC CTCCGTCTGA ATTTTT. This primer 
                     matches this site perfectly."
     primer_bind     complement(882..900)
                     /label=SJVL-629_Max=
                     /note="MSCV csgag real time primer right. Max recorded 
                     sequencing read = bases. Perfect match Tm = 52.6 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is TTCGGTTCCA AACCGAAAG. This primer matches 
                     this site completely."
     CDS             986..1376
                     /label=MA_p15
                     /note="MoMLV matrix protein from gag, without initiator 
                     methionine. As found in MSCV vectors. In wild type virus, 
                     ATG proceeds this sequence."
     primer_bind     1000..1029
                     /label=SJVL-9_Max=823
                     /note="MLV sequencing. Was HGTI-1606. Max recorded 
                     sequencing read = 823 bases. Perfect match Tm = 61.3 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is CCACTCCCTT AAGTTTGACC TTAGGTCACT . This 
                     primer matches this site perfectly."
     primer_bind     complement(1020..1048)
                     /label=SJVL-4_Max=816
                     /note="MLV sequencing. Was HGTI-1067. Max recorded 
                     sequencing read = 816 bases. Perfect match Tm = 65.4 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is ATCCGCTCGA CATCTTTCCA GTGACCTAA. This primer 
                     matches this site completely."
     primer_bind     1364..1392
                     /label=SJVL-358_Max=772
                     /note="MSCV pgk neo sequencing. Max recorded sequencing 
                     read = 772 bases. Perfect match Tm = 65.6 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     ATCCTCCCTT TATCCAGCCC TCACTCCTT. This primer matches this 
                     site perfectly."
     misc_feature    1405..1412
                     /label=NotI
     misc_feature    1413..4328
                     /label=Human CSF1R Y969F L301S
     misc_feature    2313..2315
                     /label=Mut.L301S
     misc_feature    4317..4319
                     /label=Mut.Y969F
     misc_feature    4329..4335
                     /label=NotI
     misc_feature    4364..4925
                     /label=IRES
                     /note="IRES from MSCV iresGFP. Last 6 bp are not from EMCV,
                     and are unique to this vector construction."
     primer_bind     complement(4400..4419)
                     /label=SJVL-366_Max=871
                     /note="in IRES facing left. Max recorded sequencing read = 
                     871 bases. Perfect match Tm = 57.2 C, although for oligos 
                     less than 35 bp, only the 3 prime 17 nt must match to 
                     trigger annotation. The actual sequence of the primer is 
                     CAAACGCACA CCGGCCTTAT . This primer matches this site 
                     completely."
     primer_bind     4784..4805
                     /label=SJVL-367_Max=
                     /note="in IRES facing right. Max recorded sequencing read =
                     bases. Perfect match Tm = 59.1 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is TGCCCAGAAG
                     GTACCCCATT GT. This primer matches this site perfectly."
     CDS             4932..5648
                     /label=GFP
                     /note="EGFP from Clontech pEGFP-N1 and C1, such that both 
                     polylinkers are excluded."
     primer_bind     complement(5011..5036)
                     /label=SJVL-404_Max=836
                     /note="Sequencing Primers for HRST. Max recorded sequencing
                     read = 836 bases. Perfect match Tm = 73.4 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     CTCGCCCTCG CCGGACACGC TGAACT. This primer matches this site
                     completely."
     primer_bind     5331..5354
                     /label=SJVL-497_Max=794
                     /note="GFP PCR left. Max recorded sequencing read = 794 
                     bases. Perfect match Tm = 67 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GACGGCAACA
                     TCCTGGGGCA CAAG. This primer matches this site perfectly."
     primer_bind     complement(5476..5504)
                     /label=SJVL-403_Max=772
                     /note="Sequencing Primers for HRST. Max recorded sequencing
                     read = 772 bases. Perfect match Tm = 71.8 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     GTCGCCGATG GGGGTGTTCT GCTGGTAGT. This primer matches this 
                     site completely."
     repeat_region   5501..5508
                     /label=AAV5_TR_C
                     /note="AAV serotype 5 ITR region C."
     misc_feature    5523..5549
                     /label=GFP end F
     primer_bind     complement(5598..5616)
                     /label=SJVL-498_Max=860
                     /note="GFP PCR right. Max recorded sequencing read = 860 
                     bases. Perfect match Tm = 63.2 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is CGGCGGCGGT
                     CACGAACTC. This primer matches this site completely."
     primer_bind     complement(5627..5652)
                     /label=SJVL-749_Max=
                     /note="Venus YFP pcr right. Max recorded sequencing read = 
                     bases. Perfect match Tm = 79.9 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCGGCCGCTC
                     ACTTGTACAG CTCGTCCATG CCG. This primer does not match this 
                     site completely."
     primer_bind     5753..5782
                     /label=SJVL-12_Max=851
                     /note="MLV sequencing. Was HGTI-1627. Max recorded 
                     sequencing read = 851 bases. Perfect match Tm = 54.4 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is GTACGAGCCA TAGATAAAAT AAAAGATTTT . This 
                     primer does not match this site perfectly."
     misc_feature    5764..5808
                     /label=MSCV_pPu
                     /note="Extent of polypurine tract in MSCV vectors. 1st base
                     is unique-ifier. Also occurs upstream of left LTR."
     repeat_region   5809..6321
                     /label=LTR
                     /note="MSCV left LTR, which is purely PCMV derived."
     repeat_region   5809..6178
                     /label=U3_PCMV
                     /note="U3 region from PCMV virus, which was published as 
                     modified by passaging to enable more efficient infection of
                     embryonal carcinoma cells. It has lost one of the 75 bp 
                     repeats, but other than that is nearly identical to MPSV 
                     xxwhen you take into account the errors in the genbank 
                     sequencexx."
     misc_feature    5809..5847
                     /label=LTR sequence found in reverse PCR
                     /note="LTR sequence found in reverse PCR"
     repeat_region   5809..5819
                     /label=MLV_LTR_tip
                     /note="Tip of both MLV and MPSV LTR."
     primer_bind     complement(6097..6121)
                     /label=SJVL-376_Max=494
                     /note="In MSCV U3, facing left. Max recorded sequencing 
                     read = 494 bases. Perfect match Tm = 60.6 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     AGAAGCGAGA AGCGAACTGA TTGGT. This primer matches this site 
                     completely."
     primer_bind     6104..6126
                     /label=SJVL-728_Max=
                     /note="mlv pbs primer Max recorded sequencing read = bases.
                     Perfect match Tm = 58.9 C, although for oligos less than 35
                     bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is AGTTCGCTTC
                     TCGCTTCTGT TCG. This primer matches this site perfectly."
     primer_bind     6128..6144
                     /label=SJVL-783_Max=
                     /note="MLV ECT3- U3 primer facing right. Max recorded 
                     sequencing read = bases. Perfect match Tm = 57.0 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is GCGCTTCTGC TCCCCGA. This primer matches this 
                     site perfectly."
     primer_bind     6136..6158
                     /label=SJVL-781_Max=
                     /note="MLV U3 facing right. Max recorded sequencing read = 
                     bases. Perfect match Tm = 57.4 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCTCCCCGAG
                     CTCAATAAAA GAG. This primer matches this site perfectly."
     primer_bind     6154..6174
                     /label=SJVL-780_Max=
                     /note="MLV U3, same as 725 but shifted left a little. Max 
                     recorded sequencing read = bases. Perfect match Tm = 56.3 
                     C, although for oligos less than 35 bp, only the 3 prime 17
                     nt must match to trigger annotation. The actual sequence of
                     the primer is AAGAGCCCAC AACCCCTCAC T. This primer matches 
                     this site perfectly."
     primer_bind     6157..6176
                     /label=SJVL-903_Max=
                     /note="Biotinylated oligo for MLV vector DNA purification. 
                     Max recorded sequencing read = bases. Perfect match Tm = 
                     58.5 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is AGCCCACAAC CCCTCACTCG . This 
                     primer matches this site perfectly."
     primer_bind     6158..6176
                     /label=SJVL-725_Max=
                     /note="MLV PBS pcr Max recorded sequencing read = bases. 
                     Perfect match Tm = 57.4 C, although for oligos less than 35
                     bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCCCACAACC
                     CCTCACTCG. This primer matches this site perfectly."
     primer_bind     6163..6192
                     /label=SJVL-740_Max=
                     /note="MLV U3-R junction mutagenesis plasmid. Creates Asc I
                     site. Max recorded sequencing read = bases. Perfect match 
                     Tm = 80.2 C, although for oligos less than 35 bp, only the 
                     3 prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is CAACCCCTCA CTCGGCGCGC CAGTCCTCCG 
                     . This primer matches this site perfectly."
     repeat_region   6179..6246
                     /label=R_MSCV
                     /note="The right R region from MSCV vectors, which is left 
                     half MPSV, and right half MLV, as if they used the Kpn I 
                     site."
     primer_bind     complement(6216..6240)
                     /label=SJVL-768_Max=
                     /note="More MLV LTR primers. Max recorded sequencing read =
                     bases. Perfect match Tm = 58.2 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is TGCAAGAGGG
                     TTTATTGGAT ACACG. This primer matches this site 
                     completely."
     primer_bind     complement(6233..6254)
                     /label=SJVL-769_Max=
                     /note="More MLV LTR primers. Max recorded sequencing read =
                     bases. Perfect match Tm = 55.9 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is AAGTCGGATG
                     CAACTGCAAG AG. This primer matches this site completely."
     primer_bind     6238..6259
                     /label=SJVL-726_Max=
                     /note="MLV pbs pcr Max recorded sequencing read = bases. 
                     Perfect match Tm = 58.6 C, although for oligos less than 35
                     bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GCAGTTGCAT
                     CCGACTTGTG GT. This primer matches this site perfectly."
     repeat_region   6247..6321
                     /label=U5_MLV
                     /note="U5 region of Moloney MLV."
     primer_bind     complement(6256..6275)
                     /label=SJVL-751_Max=580
                     /note="MLV U5 facing left, pcr or sequencing. Max recorded 
                     sequencing read = 580 bases. Perfect match Tm = 57.3 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is CCCAAGGAAC AGCGAGACCA . This primer matches 
                     this site completely."
     primer_bind     complement(6278..6303)
                     /label=SJVL-770_Max=
                     /note="More MLV LTR primers. Max recorded sequencing read =
                     bases. Perfect match Tm = 57.4 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is GGGTAGTCAA
                     TCACTCAGAG GAGACC. This primer matches this site 
                     completely."
     primer_bind     6278..6301
                     /label=SJVL-746_Max=
                     /note="ABI designed B2 PCR left. Max recorded sequencing 
                     read = bases. Perfect match Tm = 50.5 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     GGTCTCCTCT GAGTGATTGA CTAC. This primer matches this site 
                     perfectly."
     repeat_region   complement(6311..6321)
                     /label=MLV_LTR_tip
                     /note="Tip of both MLV and MPSV LTR."
     misc_feature    6322..6477
                     /label=muGenomic
                     /note="Murine genomic DNA flanking Abelson murine leukemia 
                     virus integrated genome, as described in V01541.1"
     misc_feature    complement(6484..6500)
                     /label=lacO
                     /note="lac operator; bound by LacI repressor to repress lac
                     operon expression"
     primer_bind     6527..6557
                     /label=SJVL-343_Max=121
                     /note="AAV 5 vector sequencing. Max recorded sequencing 
                     read = 121 bases. Perfect match Tm = 64.1 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     ATAAAGTGTA AAGCCTGGGG TGCCTAATGA G. This primer matches 
                     this site perfectly."
     primer_bind     complement(6625..6647)
                     /label=SJVL-17_Max=779
                     /note="Cloning vector sequencing primer. Was HGTI-1880. Max
                     recorded sequencing read = 779 bases. Perfect match Tm = 
                     59.4 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is gttggccgat tcattaatgc agc. This 
                     primer matches this site completely."
     misc_feature    complement(6675..7473)
                     /label=ori
                     /note="Origin of replication for some high copy bacterial 
                     plasmids. Loose match to ColE1 plasmid sequence NC_001371, 
                     from 582..1381, which contains the RNA II primer 
                     transcript. Critical t mutation defining high copy 
                     replication is in lower case."
     primer_bind     complement(6943..6963)
                     /label=SJVL-787_Max=527
                     /note="plasmid backbone primer for sequencing scAAVv2-GFP. 
                     Max recorded sequencing read = 527 bases. Perfect match Tm 
                     = 57.9 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is GGGGGAAACG CCTGGTATCT T. This 
                     primer matches this site completely."
     primer_bind     complement(6981..7002)
                     /label=SJVL-788_Max=223
                     /note="plasmid backbone primer for sequencing scAAVv2-GFP. 
                     Max recorded sequencing read = 223 bases. Perfect match Tm 
                     = 62.9 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is GGCAGGGTCG GAACAGGAGA GC. This 
                     primer matches this site completely."
     misc_feature    complement(7282..7325)
                     /label=High_copy
                     /note="Tag for High copy plasmid replication xxpUC18xx"
     misc_feature    complement(7474..8680)
                     /label=Tn3_frag
                     /note="Piece of Tn3 found in some cloning vectors, which 
                     carries beta lactamase gene. 3751..4957 of V00613.1."
     CDS             complement(7624..8481)
                     /label=bla
                     /note="B-lactamase, confers ampicillin resistance, no Pst 
                     I."
     primer_bind     8595..8629
                     /label=SJVL-717_Max=780
                     /note="Sequencing primer in pBR322, reading towards the 
                     EcoR I site. Max recorded sequencing read = 780 bases. 
                     Perfect match Tm = 71.9 C, although for oligos less than 35
                     bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is CCCGAAAAGT
                     GCCACCTGAC GTCTAAGAAA CCATT. This primer matches this site 
                     perfectly."
     misc_feature    complement(8681..8906)
                     /label=Plasmid_Frag
                     /note="Fragment of bacterial plasmid used in some cloning 
                     vectors. Loose match to the ColE1 plasmid NC_001371, 
                     1381..1647. Not sure if functionally significant."
     primer_bind     8789..8814
                     /label=SJVL-120_Max=770
                     /note="AAV-CAGG sequencing primers for TRs. Max recorded 
                     sequencing read = 770 bases. Perfect match Tm = 76.6 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is GCGTCAGCGG GTGTTGGCGG GTGTCG. This primer 
                     matches this site perfectly."
     primer_bind     8966..8985
                     /label=SJVL-977_Max=753
                     /note="RSET Sequencing. Max recorded sequencing read = 753 
                     bases. Perfect match Tm = 56.3 C, although for oligos less 
                     than 35 bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is CGGGCCTCTT
                     CGCTATTACG . This primer matches this site perfectly."
     primer_bind     9033..9056
                     /label=SJVL-21_Max=932
                     /note="Cloning vector sequencing primer, was HGTI-3334. Max
                     recorded sequencing read = 932 bases. Perfect match Tm = 
                     66.1 C, although for oligos less than 35 bp, only the 3 
                     prime 17 nt must match to trigger annotation. The actual 
                     sequence of the primer is cgccagggtt ttcccagtca cgac. This 
                     primer matches this site perfectly."
     primer_bind     9126..9152
                     /label=SJVL-344_Max=142
                     /note="AAV 5 vector sequencing. Max recorded sequencing 
                     read = 142 bases. Perfect match Tm = 73.2 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     GCCTGCCACC ATACCCACGC CGAAACA. This primer matches this 
                     site perfectly."
     misc_feature    9274..9415
                     /label=MLV_env_pPu
                     /note="142 bp upstream of MoMLV right LTR, as junk in 
                     MSCV."
     primer_bind     9300..9321
                     /label=SJVL-778_Max=
                     /note="MLV reading right into LTR. Max recorded sequencing 
                     read = bases. Perfect match Tm = 50.1 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     GATATCAGTG GTCCAGGCTC TA. This primer matches this site 
                     perfectly."
     primer_bind     complement(9316..9341)
                     /label=SJVL-8_Max=820
                     /note="MLV sequecing into genes, downstream. Was HGTI-1293,
                     or JSL-502. Max recorded sequencing read = 820 bases. 
                     Perfect match Tm = 51.1 C, although for oligos less than 35
                     bp, only the 3 prime 17 nt must match to trigger 
                     annotation. The actual sequence of the primer is tgatattgtt
                     gagtcaaaac tagagc. This primer matches this site 
                     completely."
     primer_bind     9355..9377
                     /label=SJVL-779_Max=
                     /note="MLV reading right into LTR. Max recorded sequencing 
                     read = bases. Perfect match Tm = 44.8 C, although for 
                     oligos less than 35 bp, only the 3 prime 17 nt must match 
                     to trigger annotation. The actual sequence of the primer is
                     ATAGAGTACG AGCCATAGAT AAA. This primer matches this site 
                     perfectly."
     primer_bind     9360..9389
                     /label=SJVL-12_Max=851
                     /note="MLV sequencing. Was HGTI-1627. Max recorded 
                     sequencing read = 851 bases. Perfect match Tm = 54.4 C, 
                     although for oligos less than 35 bp, only the 3 prime 17 nt
                     must match to trigger annotation. The actual sequence of 
                     the primer is GTACGAGCCA TAGATAAAAT AAAAGATTTT . This 
                     primer matches this site perfectly."
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggcggcc gcatgggccc gggcgtgctg ctgctgctgc
     1441 tggtggcgac cgcgtggcat ggccagggca ttccggtgat tgaaccgagc gtgccggaac
     1501 tggtggtgaa accgggcgcg accgtgaccc tgcgctgcgt gggcaacggc agcgtggaat
     1561 gggatggccc gccgagcccg cattggaccc tgtatagcga tggcagcagc agcattctga
     1621 gcaccaacaa cgcgaccttt cagaacaccg gcacctatcg ctgcaccgaa ccgggcgatc
     1681 cgctgggcgg cagcgcggcg attcatctgt atgtgaaaga tccggcgcgc ccgtggaacg
     1741 tgctggcgca ggaagtggtg gtgtttgaag atcaggatgc gctgctgccg tgcctgctga
     1801 ccgatccggt gctggaagcg ggcgtgagcc tggtgcgcgt gcgcggccgc ccgctgatgc
     1861 gccataccaa ctatagcttt agcccgtggc atggctttac cattcatcgc gcgaaattta
     1921 ttcagagcca ggattatcag tgcagcgcgc tgatgggcgg ccgcaaagtg atgagcatta
     1981 gcattcgcct gaaagtgcag aaagtgattc cgggcccgcc ggcgctgacc ctggtgccgg
     2041 cggaactggt gcgcattcgc ggcgaagcgg cgcagattgt gtgcagcgcg agcagcgtgg
     2101 atgtgaactt tgatgtgttt ctgcagcata acaacaccaa actggcgatt ccgcagcaga
     2161 gcgattttca taacaaccgc tatcagaaag tgctgaccct gaacctggat caggtggatt
     2221 ttcagcatgc gggcaactat agctgcgtgg cgagcaacgt gcagggcaaa catagcacca
     2281 gcatgttttt tcgcgtggtg gaaagcgcgt attcgaacct gagcagcgaa cagaacctga
     2341 ttcaggaagt gaccgtgggc gaaggcctga acctgaaagt gatggtggaa gcgtatccgg
     2401 gcctgcaggg ctttaactgg acctatctgg gcccgtttag cgatcatcag ccggaaccga
     2461 aactggcgaa cgcgaccacc aaagatacct atcgccatac ctttaccctg agcctgccgc
     2521 gcctgaaacc gagcgaagcg ggccgctata gctttctggc gcgcaacccg ggcggctggc
     2581 gcgcgctgac ctttgaactg accctgcgct atccgccgga agtgagcgtg atttggacct
     2641 ttattaacgg cagcggcacc ctgctgtgcg cggcgagcgg ctatccgcag ccgaacgtga
     2701 cctggctgca gtgcagcggc cataccgatc gctgcgatga agcgcaggtg ctgcaggtgt
     2761 gggatgatcc gtatccggaa gtgctgagcc aggaaccgtt tcataaagtg accgtgcaga
     2821 gcctgctgac cgtggaaacc ctggaacata accagaccta tgaatgccgc gcgcataaca
     2881 gcgtgggcag cggcagctgg gcgtttattc cgattagcgc gggcgcgcat acccatccgc
     2941 cggatgaatt tctgtttacc ccggtggtgg tggcgtgcat gagcattatg gcgctgctgc
     3001 tgctgctgct gctgctgctg ctgtataaat ataaacagaa accgaaatat caggtgcgct
     3061 ggaaaattat tgaaagctat gaaggcaaca gctatacctt tattgatccg acccagctgc
     3121 cgtataacga aaaatgggaa tttccgcgca acaacctgca gtttggcaaa accctgggcg
     3181 cgggcgcgtt tggcaaagtg gtggaagcga ccgcgtttgg cctgggcaaa gaagatgcgg
     3241 tgctgaaagt ggcggtgaaa atgctgaaaa gcaccgcgca tgcggatgaa aaagaagcgc
     3301 tgatgagcga actgaaaatt atgagccatc tgggccagca tgaaaacatt gtgaacctgc
     3361 tgggcgcgtg cacccatggc ggcccggtgc tggtgattac cgaatattgc tgctatggcg
     3421 atctgctgaa ctttctgcgc cgcaaagcgg aagcgatgct gggcccgagc ctgagcccgg
     3481 gccaggatcc ggaaggcggc gtggattata aaaacattca tctggaaaaa aaatatgtgc
     3541 gccgcgatag cggctttagc agccagggcg tggataccta tgtggaaatg cgcccggtga
     3601 gcaccagcag caacgatagc tttagcgaac aggatctgga taaagaagat ggccgcccgc
     3661 tggaactgcg cgatctgctg cattttagca gccaggtggc gcagggcatg gcgtttctgg
     3721 cgagcaaaaa ctgcattcat cgcgatgtgg cggcgcgcaa cgtgctgctg accaacggcc
     3781 atgtggcgaa aattggcgat tttggcctgg cgcgcgatat tatgaacgat agcaactata
     3841 ttgtgaaagg caacgcgcgc ctgccggtga aatggatggc gccggaaagc atttttgatt
     3901 gcgtgtatac cgtgcagagc gatgtgtgga gctatggcat tctgctgtgg gaaattttta
     3961 gcctgggcct gaacccgtat ccgggcattc tggtgaacag caaattttat aaactggtga
     4021 aagatggcta tcagatggcg cagccggcgt ttgcgccgaa aaacatttat agcattatgc
     4081 aggcgtgctg ggcgctggaa ccgacccatc gcccgacctt tcagcagatt tgcagctttc
     4141 tgcaggaaca ggcgcaggaa gatcgccgcg aacgcgatta taccaacctg ccgagcagca
     4201 gccgcagcgg cggcagcggc agcagcagca gcgaactgga agaagaaagc agcagcgaac
     4261 atctgacctg ctgcgaacag ggcgatattg cgcagccgct gctgcagccg aacaactttc
     4321 agttttgcgc ggccgctcac ctcacgtgac gggatcaatt ccgccccccc cctaacgtta
     4381 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4441 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     4501 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     4561 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     4621 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4681 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4741 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4801 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4861 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata
     4921 atacctgtac aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4981 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5041 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5101 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     5161 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5221 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5281 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5341 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5401 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5461 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5521 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5581 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5641 tgtacaagta aactcgaggg atccgtcgac ctgcaggcat gcaagcttca ggtagccggc
     5701 taacgttaac aaccggtacc tctagaacta tagctagcat gcgcaaattt aaagcgctga
     5761 tatcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     5821 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata
     5881 actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca
     5941 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     6001 cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     6061 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     6121 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
     6181 gccagtcctc cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     6241 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     6301 cccgtcagcg ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga
     6361 gggtaggagt cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata
     6421 ctccaatact cctgaaatag ttcattatgg acagcgcaga aagagctggg gagaattgtg
     6481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6661 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7261 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8581 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8641 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     8701 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8761 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     8821 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     8881 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     8941 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     9001 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     9061 taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     9121 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     9181 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     9241 gtgatgccgg ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg
     9301 atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa gcctatagag
     9361 tacgagccat agataaaata aaagatttta tttagtctcc agaaaaaggg gggaa
//
