LOCUS       Exported               11641 bp ds-DNA     circular SYN 12-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 18, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          6..3092
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     misc_feature    6..2842
                     /label=FastRed Cassette
                     /note="FastRed Cassette"
                     /note="From pEDO97. FastRed replaced Kanamycin resistance 
                     cassette"
     promoter        6..1387
                     /label=OLE1prom (-1382)
                     /note="OLE1prom (-1382)"
                     /note="As in pEDO97 (FastRed cassette)"
     misc_feature    1388..2146
                     /label=OLE1genome
                     /note="OLE1genome"
                     /note="As in pEDO97 (FastRed cassette)"
     exon            2153..2842
                     /label=tagRFP
                     /note="tagRFP"
                     /note="As in pEDO97 (FastRed cassette)"
     terminator      2841..3093
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3188..3212
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3740..4531
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4775..5363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5549..5689
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      6033..6227
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6293..7360)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(7794..8423)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9723..9747
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9836..9852
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9860..9983
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        10009..10039
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10093..10752
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             11094..11399
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(11440..11564)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(11583..11599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtacccttca agtgtatgta ggtatagtaa catgaacaag aacagactca agtacaagat
       61 cgcatacgaa aatggaaatg gcaatgtcac ttccacataa tcaaacacga atcctcatat
      121 caacaaggcc tgagattcta actagctcat aacaacttag ccaatagtta cttgagacta
      181 ccaaatgtat gtagaactaa agactaaggg acagagagtt cgtctaaaca ggtgaatcta
      241 gtcgttgtta tctaataaac aattcagccc caaatgcaga acacacatag agctctctat
      301 tgattcaaat tacgatctga tactgataac gtctagattt ttagggttaa agcaatcaat
      361 cacctgacga ttcaaggtgg ttggatcatg acgattccag aaaacatcaa gcaagctctc
      421 aaagctacac tctttgggat catactgaac tctaacaacc tcgttatgtc ccgtagtgcc
      481 agtacagaca tcctcgtaac tcggattgtg cacgatgcca tggctatacc caacctcggt
      541 cttggtcaca ccaggaactc tctggtaagc tagctccact ccccagaaac aaccggcgcc
      601 aaattgcgcg aattgctgac ctgaagacgg aacatcatcg tcgggtcctt gggcgattgc
      661 ggcggaagat gggtcagctt gggcttgagg acgagacccg aatccgagtc tgttgaaaag
      721 gttgttcatt ggggatttgt atacggagat tggtcgtcga gaggtttgag ggaaaggaca
      781 aatgggtttg gctctggaga aagagagtgc ggctttagag agagaattga gaggtttaga
      841 gagagatgcg gcggcgatga gcggaggaga gacgacgagg acctgcatta tcaaagcagt
      901 gacgtggtga aatttggaac ttttaagagg cagatagatt tattatttgt atccattttc
      961 ttcattgttc tagaatgtcg cggaacaaat tttaaaacta aatcctaaat ttttctaatt
     1021 ttgttgccaa tagtggatat gtgggccgta tagaaggaat ctattgaagg cccaaaccca
     1081 tactgacgag cccaaaggtt cgttttgcgt tttatgtttc ggttcgatgc caacgccaca
     1141 ttctgagcta ggcaaaaaac aaacgtgtct ttgaatagac tcctctcgtt aacacatgca
     1201 gcggctgcat ggtgacgcca ttaacacgtg gcctacaatt gcatgatgtc tccattgaca
     1261 cgtgacttct cgtctccttt cttaatatat ctaacaaaca ctcctacctc ttccaaaata
     1321 tatacacatc tttttgatca atctctcatt caaaatctca ttctctctag taaacaagaa
     1381 caaaaaaatg gcggatacag ctagaggaac ccatcacgat atcatcggca gagaccagta
     1441 cccgatgatg ggccgagacc gagaccagta ccagatgtcc ggacgaggat ctgactactc
     1501 caagtctagg cagattgcta aagctgcaac tgctgtcaca gctggtggtt ccctccttgt
     1561 tctctccagc cttacccttg ttggaactgt catagctttg actgttgcaa cacctctgct
     1621 cgttatcttc agcccaatcc ttgtcccggc tctcatcaca gttgcactcc tcatcaccgg
     1681 ttttctttcc tctggagggt ttggcattgc cgctataacc gttttctctt ggatttacaa
     1741 gtaagcacac atttatcatc ttacttcata attttgtgca atatgtgcat gcatgtgttg
     1801 agccagtagc tttggatcaa tttttttggt cgaataacaa atgtaacaat aagaaattgc
     1861 aaattctagg gaacatttgg ttaactaaat acgaaatttg acctagctag cttgaatgtg
     1921 tctgtgtata tcatctatat aggtaaaatg cttggtatga tacctattga ttgtgaatag
     1981 gtacgcaacg ggagagcacc cacagggatc agacaagttg gacagtgcaa ggatgaagtt
     2041 gggaagcaaa gctcaggatc tgaaagacag agctcagtac tacggacagc aacatactgg
     2101 tggggaacat gaccgtgacc gtactcgtgg tggccagcac actactatga gcgagctgat
     2161 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     2221 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     2281 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     2341 cagcagaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     2401 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     2461 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     2521 cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga
     2581 gatgctgtac cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt
     2641 gggcgggggc cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa
     2701 gaacctcaag atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc
     2761 cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
     2821 tagcaaactg gggcacaagt gatctagtaa catagatgac accgcgcgcg ataatttatc
     2881 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     2941 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     3001 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     3061 taagaaactt tattgccaaa tgtttgaacg atggtaccct gcatgcaagc tgataattca
     3121 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     3181 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     3241 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     3301 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     3361 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     3421 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     3481 tcgttcggtt cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga
     3541 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat
     3601 gacatgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3661 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3721 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3781 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3841 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3901 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3961 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     4021 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     4081 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     4141 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     4201 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     4261 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     4321 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     4381 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     4441 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     4501 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     4561 gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc
     4621 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     4681 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4921 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5161 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5341 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5401 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5461 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5521 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     5581 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5641 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     5701 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5761 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5821 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     5881 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     5941 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     6001 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     6061 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     6121 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     6181 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     6241 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     6301 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     6361 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     6421 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     6481 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     6541 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     6601 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     6661 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     6721 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     6781 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     6841 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     6901 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     6961 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     7021 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     7081 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     7141 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
     7201 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     7261 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     7321 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc
     7381 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     7441 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     7501 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     7561 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     7621 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     7681 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     7741 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     7801 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     7861 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
     7921 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
     7981 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
     8041 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
     8101 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
     8161 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
     8221 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
     8281 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
     8341 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
     8401 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
     8461 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
     8521 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
     8581 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
     8641 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
     8701 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
     8761 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
     8821 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
     8881 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
     8941 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
     9001 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
     9061 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
     9121 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
     9181 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
     9241 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
     9301 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
     9361 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
     9421 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
     9481 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
     9541 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
     9601 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
     9661 ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct cttttctctt
     9721 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg
     9781 acaatctgat ccaagctcaa gctaagcttg agctcgtttt cccagtcacg acgttgtaaa
     9841 acgacggcca gtgaattatc aactatgtat aataaagttg aacgagaaac gtaaaatgat
     9901 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     9961 acacaacata tccagtcact atggcggccg cattaggcac ccccaggctt tacactttat
    10021 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
    10081 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
    10141 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
    10201 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
    10261 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
    10321 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
    10381 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
    10441 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
    10501 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
    10561 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
    10621 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
    10681 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
    10741 ggcggggcgt aatctagagg atccggctta ctaaaagcca gataacagta tgcgtatttg
    10801 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
    10861 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
    10921 tcaaggcata tatgatgtca atatctccgg ttcggtaagc acaaccatgc agaatgaagc
    10981 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
    11041 ccggtttatt gaaatgaacg gctcttttgc cgacgagaac aggggctggt gaaatgcagt
    11101 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
    11161 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
    11221 cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
    11281 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
    11341 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
    11401 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
    11461 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
    11521 atatcatttt acgtttctcg ttcaactttt ctatacaaag ttgatagctt ggcgtaatca
    11581 tggtcatagc tgtttcctgt gtgaaattgt tatccgctcg ggccctctag aggatccccg
    11641 g
//
