LOCUS       pKT-mIDH1-MYC-FLAG-KAT  6307 bp ds-DNA     circular     17-SEP-2019
DEFINITION  .
KEYWORDS    "marker:IR/DR, IR/DR, KanR"                                        
FEATURES             Location/Qualifiers
     rep_origin      complement(5612..6200)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(1198..1216)
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(36..845)
                     /label="KanR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    921..1147
                     /label="IR/DR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     polyA_signal    4890..5011
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2002..3480
                     /label="mIDH1-Myc-FLAG"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             4107..4811
                     /label="TurboFP635"
                     /ApEinfo_revcolor=#a0003c
                     /ApEinfo_fwdcolor=#a0003c
     CDS             3403..3432
                     /label="Myc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5176..5403
                     /label="IR/DR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1704..1927
                     /label="hEF1alpha promoter"
                     /ApEinfo_revcolor=#3366ff
                     /ApEinfo_fwdcolor=#3366ff
     misc_feature    3533..4105
                     /label="IRES"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(3494..3510)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     misc_feature    1281..1703
                     /label="mCMV Enhancer "
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             3451..3474
                     /label="FLAG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        5113..5131
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 TTTTGCCAGT GTTACAACCA ATTAACCAAT TCTGATTAGA AAAACTCATC GAGCATCAAA
       61 TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC
      121 TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG
      181 TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA
      241 AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAAAGT
      301 TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA
      361 CTCGCATCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA
      421 TCGCTGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC
      481 AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT
      541 TTTCCGGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG
      601 ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGTC TGACCATCTC ATCTGTAACA
      661 TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA
      721 TACAAGCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA
      781 TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCCTCG ACGTTTCCCG TTGAATATGG
      841 CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGATGCA
      901 GCTGGATCCA GATCCCTATA CAGTTGAAGT CGGAAGTTTA CATACACTTA AGTTGGAGTC
      961 ATTAAAACTC GTTTTTCAAC TACTCCACAA ATTTCTTGTT AACAAACAAT AGTTTTGGCA
     1021 AGTCAGTTAG GACATCTACT TTGTGCATGA CACAAGTCAT TTTTCCAACA ATTGTTTACA
     1081 GACAGATTAT TTCACTTATA ATTCACTGTA TCACAATTCC AGTGGGTCAG AAGTTTACAT
     1141 ACACTAAGTT GACTGTGCCT TTAAACAGCT TGGAAAATTC CAGAAAATGA TGGTCTCCCT
     1201 ATAGTGAGTC GTATTAGATC CCGGGATTAC TAGTTTAAAC CTAGGATGCA TACCGGTCAA
     1261 GCTAGCGATA ATTCCTGCAG GAGTCAATGG GAAAAACCCA TTGGAGCCAA GTACACTGAC
     1321 TCAATAGGGA CTTTCCATTG GGTTTTGCCC AGTACATAAG GTCAATAGGG GGTGAGTCAA
     1381 CAGGAAAGTC CCATTGGAGC CAAGTACATT GAGTCAATAG GGACTTTCCA ATGGGTTTTG
     1441 CCCAGTACAT AAGGTCAATG GGAGGTAAGC CAATGGGTTT TTCCCATTAC TGACATGTAT
     1501 ACTGAGTCAT TAGGGACTTT CCAATGGGTT TTGCCCAGTA CATAAGGTCA ATAGGGGTGA
     1561 ATCAACAGGA AAGTCCCATT GGAGCCAAGT ACACTGAGTC AATAGGGACT TTCCATTGGG
     1621 TTTTGCCCAG TACAAAAGGT CAATAGGGGG TGAGTCAATG GGTTTTTCCC ATTATTGGCA
     1681 CATACATAAG GTCAATAGGG GTGACTAGTG GAGAAGAGCA TGCTTGAGGG CTGAGTGCCC
     1741 CTCAGTGGGC AGAGAGCACA TGGCCCACAG TCCCTGAGAA GTTGGGGGGA GGGGTGGGCA
     1801 ATTGAACTGG TGCCTAGAGA AGGTGGGGCT TGGGTAAACT GGGAAAGTGA TGTGGTGTAC
     1861 TGGCTCCACC TTTTTCCCCA GGGTGGGGGA GAACCATATA TAAGTGCAGT AGTCTCTGTG
     1921 AACATTCAAG CTTCTGCCTT CTCCCTCCTG TGAGTTTGGT AAGTCACTGA CTGTCTATGC
     1981 CTGGGAAAGG GTGGGCAGGA GATGGGGCAG TGCAGGAAAA GTGGCACTAT GAACCCTGCA
     2041 GCCCTAGGAA TGCATCTAGC GCTACCGGAC TCAGATCTCG AGCTCAAGCT TCGAATTCGT
     2101 CGACTGGATC CGGTACCGAG GAGATCTGCC GCCGCGATCG CCATGTCCAA AAAAATCAGT
     2161 GGCGGTTCTG TGGTAGAGAT GCAAGGAGAT GAAATGACAC GAATCATTTG GGAATTGATT
     2221 AAAGAGAAAC TCATTTTTCC CTACGTGGAA TTGGATCTAC ATAGCTATGA TTTAGGCATA
     2281 GAGAATCGTG ATGCCACCAA CGACCAAGTC ACCAAGGATG CTGCAGAAGC TATAAAGAAG
     2341 CATAATGTTG GCGTCAAATG TGCCACTATC ACTCCTGATG AGAAGAGGGT TGAGGAGTTC
     2401 AAGTTGAAAC AAATGTGGAA ATCACCAAAT GGCACCATAC GAAATATTCT GGGTGGCACG
     2461 GTCTTCAGAG AAGCCATTAT CTGCAAAAAT ATCCCCCGGC TTGTGAGTGG ATGGGTAAAA
     2521 CCTATCATCA TAGGTCATCA TGCTTATGGG GATCAATACA GAGCAACTGA TTTTGTTGTT
     2581 CCTGGGCCTG GAAAAGTAGA GATAACCTAC ACACCAAGTG ACGGAACCCA AAAGGTGACA
     2641 TACCTGGTAC ATAACTTTGA AGAAGGTGGT GGTGTTGCCA TGGGGATGTA TAATCAAGAT
     2701 AAGTCAATTG AAGATTTTGC ACACAGTTCC TTCCAAATGG CTCTGTCTAA GGGTTGGCCT
     2761 TTGTATCTGA GCACCAAAAA CACTATTCTG AAGAAATATG ATGGGCGTTT TAAAGACATC
     2821 TTTCAGGAGA TATATGACAA GCAGTACAAG TCCCAGTTTG AAGCTCAAAA GATCTGGTAT
     2881 GAGCATAGGC TCATCGACGA CATGGTGGCC CAAGCTATGA AATCAGAGGG AGGCTTCATC
     2941 TGGGCCTGTA AAAACTATGA TGGTGACGTG CAGTCGGACT CTGTGGCCCA AGGGTATGGC
     3001 TCTCTCGGCA TGATGACCAG CGTGCTGGTT TGTCCAGATG GCAAGACAGT AGAAGCAGAG
     3061 TCTGCCCACG GGACTGTAAC CCGTCACTAC CGCATGTACC AGAAAGGACA GGAGACGTCC
     3121 ACCAATCCCA TTGCTTCCAT TTTTGCCTGG ACCAGAGGGT TAGCCCACAG AGCAAAGCTT
     3181 GATAACAATA AAGAGCTTGC CTTCTTTGCA AATGCTTTGG AAGAAGTCTC TATTGAGACA
     3241 ATTGAGGCTG GCTTCATGAC CAAGGACTTG GCTGCTTGCA TTAAAGGTTT ACCCAATGTG
     3301 CAACGTTCTG ACTACTTGAA TACATTTGAG TTCATGGATA AACTTGGAGA AAACTTGAAG
     3361 ATCAAACTAG CTCAGGCCAA ACTTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA
     3421 GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA
     3481 ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGCCGCTCGA GGCCCCTCTC
     3541 CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG
     3601 TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG
     3661 GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG
     3721 GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT
     3781 CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC
     3841 AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA
     3901 GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCACCTCAAG CGTATTCAAC AAGGGGCTGA
     3961 AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TGCACATGCT
     4021 TTACATGTGT TTAGTCGAGG TTAAAAAACG TCTAGGCCCC CCGAACCACG GGGACGTGGT
     4081 TTTCCTTTGA AAAACACGAT GATAATATGG TGGGTGAGGA TAGCGTGCTG ATCACCGAGA
     4141 ACATGCACAT GAAACTGTAC ATGGAGGGCA CCGTGAACGA CCACCACTTC AAGTGCACAT
     4201 CCGAGGGCGA AGGCAAGCCC TACGAGGGCA CCCAGACCAT GAAGATCAAG GTGGTCGAGG
     4261 GCGGCCCTCT CCCCTTCGCC TTCGACATCC TGGCTACCAG CTTCATGTAC GGCAGCAAAA
     4321 CCTTTATCAA CCACACCCAG GGCATCCCCG ACTTCTTTAA GCAGTCCTTC CCTGAGGGCT
     4381 TCACATGGGA GAGGATCACC ACATACGAAG ACGGGGGCGT GCTGACCGCT ACCCAGGACA
     4441 CCAGCCTCCA GAACGGCTGC CTCATCTACA ACGTCAAGAT CAACGGGGTG AACTTCCCAT
     4501 CCAACGGCCC TGTGATGCAG AAGAAAACAC TCGGCTGGGA GGCCAGCACC GAGATGCTGT
     4561 ACCCCGCTGA CAGCGGCCTG AGAGGCCATA GCCAGATGGC CCTGAAGCTC GTGGGCGGGG
     4621 GCTACCTGCA CTGCTCCCTC AAGACCACAT ACAGATCCAA GAAACCCGCT AAGAACCTCA
     4681 AGATGCCCGG CTTCTACTTC GTGGACAGGA GACTGGAAAG AATCAAGGAG GCCGACAAAG
     4741 AGACCTACGT CGAGCAGCAC GAGATGGCTG TGGCCAGGTA CTGCGACCTG CCTAGCAAAC
     4801 TGGGGCACAG CTCCGGACTC AGATAACGTA CGTGCGCGCG AATGCATCCA GATCTAGGTA
     4861 CCAGGGCCCA GCTAGCTGGC CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC
     4921 AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT
     4981 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT
     5041 TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG
     5101 TATGGAATTA TCAATTAACC CTCACTAAAG GGAGACCAAG TTAAACAATT TAAAGGCAAT
     5161 GCTACCAAAT ACTAATTGAG TGTATGTAAA CTTCTGACCC ACTGGGAATG TGATGAAAGA
     5221 AATAAAAGCT GAAATGAATC ATTCTCTCTA CTATTATTCT GATATTTCAC ATTCTTAAAA
     5281 TAAAGTGGTG ATCCTAACTG ACCTAAGACA GGGAATTTTT ACTAGGATTA AATGTCAGGA
     5341 ATTGTGAAAA AGTGAGTTTA AATGTATTTG GCTAAGGTGT ATGTAAACTT CCGACTTCAA
     5401 CTGTATAGGG ATCTGGTACC ATTTAAATCT GTTCCGCTTC CTCGCTCACT GACTCGCTGC
     5461 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
     5521 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
     5581 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
     5641 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     5701 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     5761 GATACCTGTC CGCCTTTCTC CCTTCGGGAA CCGTGGCGCT TTCTCATAGC TCACGCTGTA
     5821 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     5881 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     5941 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     6001 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT
     6061 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     6121 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     6181 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
     6241 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT
     6301 AGATCCT
//