LOCUS       pJD_Donor_eGP2AP_RC     4465 bp ds-DNA     circular     19-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             2352..2948
                     /label="Puromycin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            1569..2285
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     new_primer      complement(2352..2370)
                     /label="RemovedSite_REV"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     attB            1519..1562
                     /label="attB [BxB1]"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3188..3221
                     /label="lox511"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1392..1411
                     /label="RT-PCRRev"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2256..2285
                     /label="Gibson Overlap P2A"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2222..2243
                     /label="EGFP_C_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1366..1385
                     /label="bGHpA Rev"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2952..2971)
                     /label="bGHpA Rev"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    929..948
                     /label="BB Seq Primer"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2952..2971
                     /label="bGHpA For"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      2952..3179
                     /label="bGH PA terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1412..1431
                     /label="Rev"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1752..1781
                     /label="Y66 (EGFP)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1807..1826
                     /label="eGFP-F1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     2474..2493
                     /label="pBobY seq 2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1455..1486)
                     /label="SeqFor"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2266..2285)
                     /label="eGFP R"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(join(4407..4465,1..757))
                     /label="Kan"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2286..2351
                     /label="P2A Var3"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1614..1635)
                     /label="EGFP_N_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2856..2875
                     /label="Puro-C-Seq F"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1455..1474)
                     /label="For"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1567..1585)
                     /label="eGFPR_N"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      complement(1158..1385)
                     /label="bGH PA terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1392..1411)
                     /label="Insert Seq 1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
       61 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      121 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      181 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
      241 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
      301 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
      361 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
      421 attcttctaa tacctggaat gctgttttcc cagggatcgc agtggtgagt aaccatgcat
      481 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
      541 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
      601 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
      661 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
      721 gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt
      781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
      841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
      901 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
      961 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     1021 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     1081 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacA
     1141 AGCTTGCATG CCTGCAGcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
     1201 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     1261 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     1321 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     1381 agtcgACCGG TGACTGACGT AACCACCCTG ATCGACGGGG AGTGTACTAG TATGAGGTCA
     1441 CAAGTGTGAC TGAAACGCGT GCTCTACGAC TATGCTCTTC CCAACTGCTA GCCGTATCAT
     1501 CGAGCTCACG TGAAGCTTcc ggcttgtcga cgacggcggt ctccgtcgtc aggatcatcc
     1561 gggaattcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1621 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1681 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1741 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1801 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1861 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1921 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1981 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2041 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcAgcgtgc
     2101 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2161 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2221 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctGt
     2281 acaagGGAAG CGGAGCGACG AATTTTAGTC TACTGAAACA AGCGGGAGAC GTGGAGGAAA
     2341 ACCCTGGACC Taccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     2401 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     2461 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     2521 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     2581 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     2641 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     2701 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     2761 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     2821 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcactg
     2881 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     2941 gtgcctgaCC Tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3001 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     3061 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     3121 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt
     3181 taattaaATA ACTTCGTATA ATGTATACTA TACGAAGTTA TggcgcgccG TTAACgatat
     3241 ccatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3301 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3361 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3421 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3481 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3541 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3601 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3661 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3721 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3781 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3841 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3901 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3961 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4021 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4081 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4141 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4201 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4261 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4321 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4381 atatatgagt aaacttggtc tgacagttag aaaaactcat cgagcatcaa atgaaactgc
     4441 aatttattca tatcaggatt atcaa
//