LOCUS       D15\AAV\EF1A\dbl        8592 bp    DNA     circular     15-DEC-2009
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|528203166|
COMMENT     VNTDBDATE|537726585|
COMMENT     LSOWNER|
COMMENT     VNTNAME|D15 AAV EF1A dble flox PSD95-EGFP WPRE|
COMMENT     VNTAUTHORNAME|Denise Piscopo|
FEATURES             Location/Qualifiers
     misc_feature    complement(89..122)
                     /vntifkey="21"
                     /label=loxP
     misc_feature    5554..5587
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(5..38)
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    5470..5503
                     /vntifkey="21"
                     /label=lox2722
     misc_feature    137..745
                     /vntifkey="21"
                     /label=WPRE
     promoter        4176..5439
                     /vntifkey="29"
                     /label=EF-1a
     rep_origin      3348..4015
                     /vntifkey="33"
                     /label=pUC\ori
     misc_feature    2340..3197
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      1515..1821
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    1283..1423
                     /vntifkey="21"
                     /label=R-ITR
     polyA_signal    765..1243
                     /vntifkey="25"
                     /label=hGH\polyA
     misc_feature    4021..4161
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    complement(6331..8571)
                     /vntifkey="21"
                     /label=PSD-95
     misc_feature    complement(6319..6324)
                     /vntifkey="21"
                     /label=kozak
BASE COUNT     1706 a      2305 c      2394 g      2187 t 
ORIGIN
        1 tagcataact tcgtataaag tatcctatac gaagttattt gccttaaccc agaaattatc 
       61 actgttattc tttagaatgg tgcaaagaat aacttcgtat aatgtatgct atacgaagtt 
      121 atgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 
      181 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
      241 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
      301 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
      361 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
      421 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
      481 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
      541 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt 
      601 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
      661 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
      721 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg 
      781 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct 
      841 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat 
      901 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg 
      961 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc 
     1021 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc 
     1081 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt 
     1141 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt 
     1201 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac 
     1261 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg 
     1321 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc 
     1381 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat 
     1441 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg 
     1501 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     1561 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     1621 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     1681 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat 
     1741 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     1801 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag 
     1861 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     1921 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct 
     1981 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 
     2041 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 
     2101 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 
     2161 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 
     2221 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     2281 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 
     2341 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     2401 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 
     2461 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 
     2521 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 
     2581 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 
     2641 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 
     2701 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 
     2761 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 
     2821 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 
     2881 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 
     2941 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 
     3001 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 
     3061 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 
     3121 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 
     3181 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 
     3241 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 
     3301 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 
     3361 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 
     3421 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 
     3481 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 
     3541 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 
     3601 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 
     3661 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 
     3721 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 
     3781 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 
     3841 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     3901 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 
     3961 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
     4021 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
     4081 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
     4141 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt 
     4201 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat 
     4261 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 
     4321 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 
     4381 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 
     4441 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc 
     4501 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat 
     4561 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
     4621 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
     4681 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
     4741 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
     4801 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
     4861 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
     4921 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
     4981 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     5041 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     5101 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     5161 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     5221 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     5281 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     5341 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     5401 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc 
     5461 gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga 
     5521 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg 
     5581 aagttatggc gcgccctatt acttgtacag ctcgtccatg ccgagagtga tcccggcggc 
     5641 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc 
     5701 ggactggtgg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt 
     5761 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt 
     5821 gaagttggcc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt 
     5881 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt 
     5941 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta 
     6001 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga 
     6061 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta 
     6121 gctgaaggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa 
     6181 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt 
     6241 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc 
     6301 ctcgcccttg ctcaccatgg tggcgaccgg cagtctctct cgggctggga cccagatgta 
     6361 ggggcctgag aggtcttcaa tgacacgttt cactttgtga tagatctctt caaagctgtc 
     6421 gccctctacg atggctgaga agcactctgt gaactcctgc tccagcttcg tggctctgtc 
     6481 gaaggctttc cgggcttgct cctctgtgat ccgcttattg atctctagca cattctccag 
     6541 ggagcgggga cggatgaaga tggcgatggg gtgcaggtgg gccgcctgca gccgccgcac 
     6601 ggcattggcc gagacatcga ggatgcagtg cttcccctgc tctgctacct ctcgcacaga 
     6661 ctggacgctg gtcccataga ggtggctgtt gtactggcca gcctcaatga acttgtgtgc 
     6721 ctggatgtcc ttctccattt tctcccggga ggagacaaag tggtaatccc ggccgtctat 
     6781 ctcatattcc cgcttaggac gtgtcgtatg agggacacag gatccaaact tgtcggggaa 
     6841 ctcggagaga agatcatcgt tggcacggtc tttggtgggt ccaaggatga tgatgggacg 
     6901 agcatagtgc acttccatct gggtcaccgt ctcatagctc agaaccgagt cttctcgacc 
     6961 ctgtgatcca gagctggagc cccagtcctt ggcctttaac cttgaccact ctcgtcgctc 
     7021 gacccgccgt ttgctgggaa tgaagccaat gtcgtcggtc tcactgtcgg agtggacccg 
     7081 ccgtgcttgc caccactctt cgtcaccagc gtcaatgaca tgaagcacat ccccgaagcg 
     7141 gaagctcagg gcctggctca agaaaccgca gtccttggtc ttgtcgtaat caaacagggc 
     7201 cctaatgtag aagcccctct tggggttgct tcgcaaggat gcagtccctg agcctaggct 
     7261 actattcatg agctgttccc gaagatcatg gatcttggcc tcgaatcgac tatactcttc 
     7321 tggtttatac tgagcgatga tcgtgaccgt ctgacccgca ttcttcaggg caatggcagc 
     7381 ctgttcgtga ctggcattgc ggaggtcaac accattgacc gacaggatct ggtccccctt 
     7441 ccgtagctcc ccactgaggt cggctggacc cccagcaagg atgaaggaga tgaagatgcc 
     7501 ttcaccatcc tcgccgccca caatgttgaa gcccaggccg gtggagcccc gatggatcac 
     7561 gatccgcctt ggttcccggg gaatgtcttc ctcccccagc aggtccttgg ccacagggga 
     7621 gtagcgccga ggggaagtgg gggtcatggc tgtggggtag tcagtgccca agtagctgct 
     7681 atgactgatc tcattgtcca ggtgctgaga atacgaggtt gtgatgtctg ggggagcata 
     7741 gctgtcactc aggtaggcat tgctgggctt ggccaccttt aggtacacaa cgtcatatgt 
     7801 gttcttcagg gctgccacgg catcctcgtg catgacgtcc tccagcccca cactgttgac 
     7861 cgccaggatc ttgtctccaa tctgcaacct gccatccttg tgggcagcac ctccttcgat 
     7921 gatctttgtt acatagatgc tgttatctcc agggatgtgc tggttcccaa cgccccccgc 
     7981 aatgctgaag ccaagtcctt taggcccttt gatgagtttg atctccatga ccttttcggc 
     8041 tgggggtttc cggcgcatga catagaggcg aacgatggaa cctgcctctt tgagggcctc 
     8101 caccgcagct gaatgggtca cctcccgaac atccacttca tttacaaaca ggatgctgtc 
     8161 attgaccctg aggcggccat cctgggctgc agccccacca ggaatgatct tggtgataaa 
     8221 aatggacggg tcgtcaccga tgtgcgggtt gtcagtgcca cctgcgatgc tgaagcccag 
     8281 acctgagtta cccctttcca atgtgatctc ctcatactcc atctccccct ctgttccatt 
     8341 cacctgcaac tcatatcctg gggcttctag ggtgtccgtg ttgacaatca cagggggaga 
     8401 attggcctgg ttggggaggt gggccgggct gtgttccaga gggggcgtgt cttcatcttg 
     8461 gtagcggtat ttcttggttg tcactataca gagacagtcc atgttggggg gcctggccgc 
     8521 ggcggcgagt agggggctct gacttcattg gagttaatcg aattcgaagc tccggtggat 
     8581 cccgggcccg cg 
//
