LOCUS       Exported                2284 bp ds-DNA     circular SYN 20-MAY-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          352..431
                     /organism="synthetic DNA construct"
     misc_feature    13..330
                     /label=U6 promoter
     misc_feature    331
                     /label=Editing History Insertion
     promoter        332..350
                     /label=BsmBI cassette
     promoter        complement(332..337)
                     /label=BsmBI
     promoter        345..350
                     /label=BsmBI
     misc_feature    352..431
                     /label=Extracted region from St1Cas9_LMD-9_sgRNA_pUC19...
     misc_RNA        352..431
                     /label=St1Cas9 sgRNA
     misc_feature    433..438
                     /label=Editing History Insertion
     misc_feature    complement(476..1149)
                     /label=pUC origin
     promoter        complement(1294..2154)
                     /label=bla CDS
     misc_feature    complement(2155..2253)
                     /label=bla promoter
     misc_feature    2189..2211
                     /label=oS280 primer
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc cgagacgatt aatgcgtctc ggtctttgta
      361 ctctggtacc agaagctaca aagataaggc ttcatgccga aatcaacacc ctgtcatttt
      421 atggcagggt gtttttttaa gcttgggccg ctcgaggtac ctctctacat atgacatgtg
      481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      541 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      601 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      661 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      721 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      781 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      841 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      901 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      961 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1021 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1081 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1141 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1201 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1261 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1321 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1381 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1441 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1501 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1561 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1621 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1681 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1741 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1801 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1861 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1921 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1981 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2041 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2101 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2161 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2221 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2281 acct
//
