LOCUS       Exported                2296 bp ds-DNA     circular SYN 20-MAY-2013
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..330
                     /label=U6 promoter
     misc_feature    331
                     /label=Editing History Insertion
     promoter        332..350
                     /label=BsmBI cassette
     promoter        complement(332..337)
                     /label=BsmBI
     promoter        345..350
                     /label=BsmBI
     misc_RNA        352..443
                     /label=St3Cas9 sgRNA
     misc_feature    445..450
                     /label=Editing History Insertion
     misc_feature    complement(488..1161)
                     /label=pUC origin
     promoter        complement(1306..2166)
                     /label=bla CDS
     misc_feature    complement(2167..2265)
                     /label=bla promoter
     misc_feature    2201..2223
                     /label=oS280 primer
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc cgagacgatt aatgcgtctc ggttttagag
      361 ctgtgttgtt tgttaaaaca acacagcgag ttaaaataag gcttagtccg tactcaactt
      421 gaaaaggtgg caccgattcg gtgttttttt aagcttgggc cgctcgaggt acctctctac
      481 atatgacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      961 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1201 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1261 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1321 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1381 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1441 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1501 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1561 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1621 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1681 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1741 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1801 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1861 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1921 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1981 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2041 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2101 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2161 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2221 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2281 ccgaaaagtg ccacct
//
