LOCUS       Exported                3711 bp ds-DNA     circular SYN 04-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Feb 5, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             697..717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             726..743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    772..996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1067..1083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1091..1107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1115..1145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1160..1181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1469..2057)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2228..3088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3089..3193)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(3463..3711,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gggcaacatc
      421 aacgacctga tcagagagat caagaacaag gactacaccg tgaagctgag cggcaccgac
      481 agcaacagca tcacccagct gatcatcaga gtgaacaacg acggcaacga gtacgtgatc
      541 agcgagagcg agaacgagag catcgtggag aagttcatca gcgccttcaa gaacggctgg
      601 aaccaggagt acgaggacga ggaggagttc tacaacgaca tgcagaccat caccctgaag
      661 agcgagctga acggatccgg cggtggaggc agtgggccca agaagaagag gaaagtctga
      721 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
      781 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      841 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      901 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
      961 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     1021 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     1081 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1141 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1201 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1261 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1321 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1381 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1441 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1501 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1561 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1621 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     1681 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1741 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1801 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1861 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1921 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1981 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2041 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2101 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2161 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2221 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2281 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2341 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2401 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2461 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2521 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2581 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2641 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2701 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2761 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2821 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2881 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2941 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3001 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3061 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3121 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3181 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     3241 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     3301 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     3361 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     3421 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     3481 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     3541 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3601 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3661 gggtggacta tttacggtaa actgcccact tggcagtaca tcaagtgtat c
//
