LOCUS       Exported               10824 bp ds-DNA     circular SYN 06-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10824)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 2, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10824
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    550..729
                     /label=cPPT
                     /note="gene=""cPPT"" - vntifkey=""34"" - label=""cPPT"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     550..729"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    576..693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     609..630
                     /label=QB2741
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     misc_feature    705..728
                     /label=cPPT_5'seq
                     /note="vntifkey=""27"" - label=""cPPT_5'seq"" - 
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     705..728"
                     /note="color: #dc143c; direction: RIGHT"
     primer_bind     738..770
                     /label=EcoR1_TetO_Fw
                     /note="color: black; sequence: 
                     gcttgatatcgaattcgttacataacttacggt"
     primer_bind     738..770
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     GCTTGATATCGAATTcgttacataacttacggt"
     primer_bind     738..769
                     /label=EcoTetO_Fw
                     /note="color: black; sequence: 
                     gcttgatatcgaattcgttacataacttacgg"
     enhancer        753..1056
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        1057..1260
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    1262..1280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     primer_bind     complement(1278..1321)
                     /label=TetO_EcoR1_Rv
                     /note="color: black; sequence: 
                     tgacgcgtgagaattcgacgatctctatcactgatagggagatct"
     primer_bind     complement(1279..1312)
                     /label=EcoTetO_Rv
                     /note="color: black; sequence: 
                     gtgtatacgggaattcgacgatctctatcactgatagggagatc"
     protein_bind    1283..1301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     primer_bind     1342..1372
                     /label=CD19_Spe1.For
                     /note="color: black; sequence: 
                     GTACCGCGGGCCCGGATGGAGACAGACACAC"
     primer_bind     1342..1372
                     /label=Fragment 1.FOR (2)
                     /note="color: black; sequence: 
                     GTACCGCGGGCCCGGATGGAGACAGACACAC"
     misc_feature    1357..1419
                     /label=IgK_signal sequence
                     /note="color: #ff0000"
     CDS             1357..1419
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /note="color: #993366
                     Cleavage site after base 1416"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     primer_bind     1357..1379
                     /label=162CD19_Fw
                     /note="color: black; sequence: 
                     ggcctcatATGGAGACAGACACACTCCTGCT"
     CDS             1420..1446
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     misc_feature    1462..2280
                     /label=putative ECD of human CD19
                     /note="color: #00ffff"
     misc_feature    1927
                     /label=mutation c to g in original Open Biosys construct,
                     /note="color: #c0c0c0"
     primer_bind     complement(2263..2288)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: ACAGCATTCTTCCAGCCACCAGTCCT"
     primer_bind     2274..2296
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: CTGGAAGAATGCTGTGGGCCAGG"
     misc_feature    2280
                     /label=c_myc
                     /note="color: #ff0000"
     misc_feature    2281..2430
                     /label=PDGFR TM domain
                     /note="color: #0000ff; direction: RIGHT"
     primer_bind     complement(2410..2430)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     ggcgaattttaCTAACGTGGCTTCTTCTGCCAAAG"
     primer_bind     complement(2413..2444)
                     /label=CD19_Spe1.Rev
                     /note="color: black; sequence: 
                     ccgctggcactagtACGTGGCTTCTTCTGCCA"
     primer_bind     complement(2413..2444)
                     /label=Fragment 1.REV (2)
                     /note="color: black; sequence: ccgctggcACGTGGCTTCTTCTGCCA"
     primer_bind     complement(2420..2446)
                     /label=162CD19_Rv
                     /note="color: black; sequence: tgccgctggcactagtACGTGGCTTCT"
     primer_bind     2424..2454
                     /label=Spe1_P2A_Not1.FOR
                     /note="color: black; sequence: 
                     GCCACGTactagtgccagcggcagcggcgcc"
     primer_bind     2435..2454
                     /label=Fragment 2.FOR (2)
                     /note="color: black; sequence: GCCACGTgccagcggcagcggcgcc"
     CDS             2452..2508
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 2505"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(2493..2517)
                     /label=Spe1_P2A_Not1.REV
                     /note="color: black; sequence: 
                     atggtagctccCCGGgcggccgcaggcccggggttttct"
     primer_bind     complement(2493..2512)
                     /label=Fragment 2.REV (2)
                     /note="color: black; sequence: 
                     atggtagctccCCGGaggcccggggttttct"
     primer_bind     2500..2536
                     /label=mcherry_Fw_AGG
                     /note="color: black; sequence: 
                     cccgggcctgcggccgccATGGTGAGCAAGGGCGAGG"
     primer_bind     2500..2533
                     /label=mcherry_Fw
                     /note="color: black; sequence: 
                     cccgggcctgcggccgccATGGTGAGCAAGGGCG"
     primer_bind     2509..2536
                     /label=EF1amC_Fw
                     /note="color: black; sequence: 
                     aattctcacgcggccgccaccATGGTGAGCAAGGGCgagg"
     misc_feature    2518..3227
                     /label=mCherry
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     complement(2720..2739)
                     /label=QB3350
                     /note="color: black; sequence: GGAGCCGTACATGAACTGAG"
     primer_bind     complement(3205..3228)
                     /label=EF1amC_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaaTTACTTGTACAGCTCGTCCATGCC"
     primer_bind     complement(3208..3249)
                     /label=mcherry_Rv
                     /note="color: black; sequence: 
                     TCAAGCTTGCATGCCtgcaggTTACTTGTACAGCTCGTCCAT"
     primer_bind     complement(3229..3248)
                     /label=pHR_SBfIhomo_mCherry_rev
                     /note="color: black; sequence: 
                     gcttgcatgcctgcaAGCTTGCATGCCTGCAGG"
     misc_feature    3256..3278
                     /label=1167_seq
                     /note="gene=""**"" - vntifkey=""27"" - label=""1167_seq"" -
                     apeinfo_fwdcolor=""pink"" - apeinfo_revcolor=""pink"" - 
                     2158..2180"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    3265..3856
                     /label=WPRE
                     /note="label=WPRE - ApEinfo_fwdcolor=#f56e9a - 
                     ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 12144..12735"
                     /note="color: #dc143c; direction: RIGHT"
     misc_feature    3266..3854
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(3319..3339)
                     /label=QB0112
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(3737..3748)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2"
                     /translation="IEGR"
     LTR             3917..4150
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(4175..4193)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4982..5086
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     gene            5086..5946
                     /label=bla (AmpR)
                     /label=bla\(AmpR)
                     /note="gene=""AmpR"" - note=""CDS "" - vntifkey=""4"" - 
                     label=""bla\(AmpR)"" - apeinfo_fwdcolor=""#9e9100"" - 
                     apeinfo_revcolor=""#9e9100"" - 3989..4849"
                     /note="color: #ff4500; direction: RIGHT"
     CDS             5087..5947
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5087 .. 5155 / #ccffcc / signal sequence
                        2: 5156 .. 5947 / #ccffcc
                     Cleavage site after base 5155"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            5087..5947
                     /label=bla (AmpR)
                     /label=bla\(AmpR)
                     /note="gene=""AmpR"" - note=""CDS "" - vntifkey=""4"" - 
                     label=""bla\(AmpR)"" - apeinfo_fwdcolor=""#9e9100"" - 
                     apeinfo_revcolor=""#9e9100"" - 3989..4849"
                     /note="color: #ff4500; direction: RIGHT"
     rep_origin      6118..6706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        6952..7281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      7132..7267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     intron          8415..8480
                     /label=small t intron
                     /note="simian virus 40 (SV40) small t antigen intron"
                     /note="color: #------"
     CDS             8610..8630
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    9055..9189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             9358..9991
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    10038..10163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    join(10655..10824,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggtc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
      601 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
      661 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
      721 ccagtttgga tcgataagct tgatatcgaa ttcgttacat aacttacggt aaatggcccg
      781 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      841 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      901 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      961 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1021 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1081 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1141 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1201 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1261 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgaat tctcacgcgt
     1321 ctgcaggata tcaagcttgc ggtaccgcgg gcccggatgg agacagacac actcctgcta
     1381 tgggtactgc tgctctgggt tccaggttcc actggtgact atccatatga tgttccagat
     1441 tatgctgggg cccagccggc caggcccgag gaacctctag tggtgaaggt ggaagaggga
     1501 gataacgctg tgctgcagtg cctcaagggg acctcagatg gccccactca gcagctgacc
     1561 tggtctcggg agtccccgct taaacccttc ttaaaactca gcctggggct gccaggcctg
     1621 ggaatccaca tgaggcccct ggccatctgg cttttcatct tcaacgtctc tcaacagatg
     1681 gggggcttct acctgtgcca gccggggccc ccctctgaga aggcctggca gcctggctgg
     1741 acagtcaatg tggagggcag cggggagctg ttccggtgga atgtttcgga cctaggtggc
     1801 ctgggctgtg gcctgaagaa caggtcctca gagggcccca gctccccttc cgggaagctc
     1861 atgagcccca agctgtatgt gtgggccaaa gaccgccctg agatctggga gggagagcct
     1921 ccgtgtctcc caccgaggga cagcctgaac cagagcctca gccaggacct caccatggcc
     1981 cctggctcca cactctggct gtcctgtggg gtaccccctg actctgtgtc caggggcccc
     2041 ctctcctgga cccatgtgca ccccaagggg cctaagtcat tgctgagcct agagctgaag
     2101 gacgatcgcc cggccagaga tatgtgggta atggagacgg gtctgttgtt gccccgggcc
     2161 acagctcaag acgctggaaa gtattattgt caccgtggca acctgaccat gtcattccac
     2221 ctggagatca ctgctcggcc agtactatgg cactggctgc tgaggactgg tggctggaag
     2281 aatgctgtgg gccaggacac gcaggaggtc atcgtggtgc cacactcctt gccctttaag
     2341 gtggtggtga tctcagccat cctggccctg gtggtgctca ccatcatctc ccttatcatc
     2401 ctcatcatgc tttggcagaa gaagccacgt actagtgcca gcggcagcgg cgccacaaac
     2461 ttctctctgc taaagcaagc aggtgatgtt gaagaaaacc ccgggcctgc ggccgccatg
     2521 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2581 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2641 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     2701 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     2761 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     2821 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     2881 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     2941 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3001 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3061 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3121 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3181 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaacc tgcaggcatg
     3241 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     3301 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3361 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3421 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3481 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3541 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3601 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3661 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3721 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3781 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3841 ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg tagatcttag ccacttttta
     3901 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     3961 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4021 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4081 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4141 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     4201 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     4261 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     4321 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     4381 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     4441 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     4501 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     4561 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     4621 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     4681 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctagctctg atgccgcata
     4741 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4801 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4861 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4921 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4981 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5041 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5101 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5161 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5221 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5281 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5341 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5401 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5461 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5521 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5581 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5641 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5701 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5761 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5821 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5881 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5941 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6001 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6061 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6121 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6181 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6241 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6301 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6361 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6421 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6481 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6541 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6601 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6661 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6721 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6781 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6841 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6901 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     6961 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7021 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7081 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7141 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7201 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7261 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     7321 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     7381 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     7441 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     7501 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     7561 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     7621 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     7681 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     7741 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     7801 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     7861 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     7921 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     7981 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     8041 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     8101 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     8161 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     8221 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     8281 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     8341 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     8401 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     8461 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     8521 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     8581 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     8641 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     8701 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     8761 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     8821 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     8881 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     8941 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     9001 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9061 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9121 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9181 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     9241 cctattacca ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga
     9301 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     9361 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     9421 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     9481 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     9541 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     9601 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     9661 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     9721 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     9781 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     9841 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9901 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9961 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
    10021 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
    10081 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
    10141 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
    10201 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
    10261 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
    10321 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
    10381 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
    10441 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
    10501 cggccggtga tcttcagacc tggacgatat atatgaggga caattggaga agtgaattat
    10561 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    10621 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    10681 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    10741 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    10801 atctgttgca actcacagtc tggg
//
