LOCUS       Exported               12574 bp ds-DNA     circular SYN 03-OCT-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Lim Lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 2, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    96..7517
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             1231..2091
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1429..2088
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2186..2868
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        3084..3410
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        3229..3425
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      5105..5123
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(5138..5329)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      5199..5330
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      5199..5329
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   7518..7697
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     7577..7598
                     /label=QB2741
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     promoter        7740..8107
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
                     /note="color: #99ccff; direction: RIGHT"
     primer_bind     8127..8170
                     /label=NcadGFP_Fw
                     /note="color: black; sequence: 
                     tgcaattctcacgcgtgccgccaccatgtgccggatagcgggag"
     misc_feature    8152..10870
                     /label=N-cad
                     /note="mouse Cadherin-2"
                     /note="color: #84a4c0; direction: RIGHT"
     primer_bind     complement(8732..8751)
                     /label=NcadRv3
                     /note="color: black; sequence: tggcccagtgacgctgtatc"
     primer_bind     complement(9476..9497)
                     /label=NcadRv2
                     /note="color: black; sequence: ggttttaccactgtgactagcc"
     primer_bind     complement(10216..10237)
                     /label=NcadRv1
                     /note="color: black; sequence: ggatggaaatgttggacttggg"
     primer_bind     10735..10748
                     /label=NcP2A_Fw
                     /note="color: black; sequence: 
                     tgacgactCGCGGccggccagcggcagcggcgccac"
     primer_bind     10863..10898
                     /label=NcP2A_Fw
                     /note="color: black; sequence: 
                     tgacgactCGCGGccggccagcggcagcggcgccac"
     primer_bind     10879..10896
                     /label=GlPBFP_Fw
                     /note="color: black; sequence: ACCCCGAgccagcggcagcggcgcc"
     misc_feature    10879
                     /label=Brn2
                     /note="color: #ff8a29"
     CDS             10894..10950
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(10936..10961)
                     /label=NcP2A_Rv
                     /note="color: black; sequence: gtctcCATatgaggcccggggttttc"
     primer_bind     complement(10937..10957)
                     /label=RhoAP2A_Rv
                     /note="color: black; sequence: 
                     CCCTTGCTCACCATatgaggcccggggtttt"
     primer_bind     complement(10937..10957)
                     /label=DTRP2A_Rv
                     /note="color: black; sequence: 
                     CCCTTGCTCACCATatgaggcccggggtttt"
     primer_bind     complement(10937..10957)
                     /label=Rock2_Rv
                     /note="color: black; sequence: 
                     CCCTTGCTCACCATatgaggcccggggtttt"
     primer_bind     10947..10974
                     /label=PGlig_Fw
                     /note="color: black; sequence: 
                     gcctcatATGgagaccgACACCCTCTtg"
     CDS             10954..11883
                     /codon_start=1
                     /note="color: #000000"
                     /translation="METDTLLLWVLLLWVPGSTGDMVSKGEELFTGVVPILVELDGDVN
                     GHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHD
                     FFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKL
                     EYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY
                     LSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKNAVGQDTQEVIVVPHSLPFKV
                     VVISAILALVVLTIISLIILIMLWQKKPR"
     misc_feature    10954..11016
                     /label=signal sequence (Ig kappa chain V-III region mouse
                     /note="color: #ff0000"
     primer_bind     10954..10967
                     /label=EFGFPlig_Fw
                     /note="color: black; sequence: 
                     aattctcacgcggccgcCACCATGGAGACCGACAC"
     misc_feature    10954..10957
                     /label=mCherry
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    10954
                     /label=eGFP
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    11017..11733
                     /label=EGFP
                     /note="color: #00ff00"
     primer_bind     complement(11342..11363)
                     /label=EGFPmidRvseq
                     /note="color: black; sequence: TCGAACTTAACTTCAGCCCTGG"
     primer_bind     complement(11439..11460)
                     /label=EGFPRvseq_mid
                     /note="color: black; sequence: GCTGTTGTAGTTGTACTCCAGC"
     primer_bind     complement(11487..11506)
                     /label=QB2643
                     /note="color: black; sequence: CCTTGATGCCGTTCTTCTGC"
     primer_bind     11636..11663
                     /label=GmWnt3a_Fw
                     /note="color: black; sequence: 
                     CCCTCTCCAAGGATCCTAACGAAAAAAG"
     primer_bind     complement(11716..11733)
                     /label=mcherry_Rv
                     /note="color: black; sequence: 
                     TCAAGCTTGCATGCCtgcaggTTACTTGTACAGCTCGTCCAT"
     misc_feature    11800..11868
                     /label=transmembrane
                     /note="color: #99ccff"
     primer_bind     complement(11853..11883)
                     /label=GFPligB_Rv
                     /note="color: black; sequence: 
                     ccgctggcTCGGGGTTTTTTTTGCCACAGCATGATAAGT"
     primer_bind     complement(11854..11903)
                     /label=PGlig_Rv
                     /note="color: black; sequence: 
                     GTGggagttgcggccgcttaTCGGGGTTTTTTTTGCCACAGCATGATaag"
     primer_bind     complement(11860..11914)
                     /label=TGFPlig_Rv
                     /note="color: black; sequence: 
                     TCAAGCTTGCATGCCtgcaggTTATCGGGGTTTTTTTTGCCACAGCAT"
     primer_bind     complement(11863..11903)
                     /label=GFPlig_Rv
                     /note="color: black; sequence: 
                     GTGggagttgcggccgcTTATCGGGGTTTTTTTTGCCACAG"
     primer_bind     complement(11868..11900)
                     /label=EFGFPlig_Rv
                     /note="color: black; sequence: 
                     ggagttgcggccTTAattaaTTATCGGGGTTTTTTTTGC"
     misc_feature    11887
                     /label=Brn2
                     /note="color: #ff8a29"
     enhancer        11983..12574
                     /label=WPRE
                     /note="color: #a6acb3"
     primer_bind     complement(12037..12057)
                     /label=QB0112
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cccgtggaat tatcacctcg
     7741 agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga
     7801 gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag tttactccct
     7861 atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac
     7921 agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga
     7981 gaacgtataa gctttgctta tgtaaaccag ggcgcctata aaagagtgct gattttttga
     8041 gtaaacttca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     8101 tcgtaaagaa ttcccgtata caccggtgca attctcacgc gtgccgccac catgtgccgg
     8161 atagcgggag cgccgcggac cctgctgccg cttctggcgg ccttgcttca ggcgtctgtg
     8221 gaggcttctg gtgaaattgc attatgcaag actggatttc ctgaagatgt ttacagcgca
     8281 gtcttaccga aggatgtgca cgaaggacag ccccttctca atgtgaaatt cagcaactgc
     8341 aatagaaaaa ggaaagttca gtatgaaagc agcgagccag cagatttcaa ggtggacgag
     8401 gacggcacgg tgtatgctgt gagaagcttc cctctcactg cagagcaggc aaagttcctg
     8461 atatatgccc aagacaaaga aacccaggaa aagtggcagg tagctgtaaa cctgagccgg
     8521 gagccaaccc tgactgagga gcctatgaag gaaccacatg aaattgaaga aatagtattc
     8581 cctagacaac ttgccaagca cagtggagct ctacaaaggc agaagagaga ctgggtcatc
     8641 ccgccaatca acttgccaga aaactccaga ggaccctttc ctcaagagct tgtcagaatc
     8701 aggtctgata gagataaaaa cctttccctg agatacagcg tcactgggcc aggagctgac
     8761 cagcctccaa cgggcatctt cattatcaac cccatctcag gacagctgtc agtcacaaag
     8821 cctctggatc gagagctgat agcccggttt cacttgagag cacatgcagt ggacatcaat
     8881 ggcaatcaag tggagaaccc cattgacatt gtcatcaatg ttattgacat gaatgataac
     8941 agacctgagt ttctgcacca ggtttggaat gggtctgttc cagagggatc aaagcctggg
     9001 acgtatgtga tgacggtcac tgccattgat gcggatgatc caaatgccct gaatggaatg
     9061 ctgcggtaca ggatcctgtc ccaggcgccc agcacacctt cacccaacat gtttacaatc
     9121 aacaatgaga ctggggacat catcactgtg gcagctggtc tggatcgaga gaaagtgcaa
     9181 cagtatacgt taataattca agccacagac atggaaggca atcccactta tggcctttca
     9241 aacacagcca cagccgtcat cacggtgaca gatgtcaatg acaatcctcc agagtttact
     9301 gccatgactt tctacggaga agtccctgag aacagggtgg acgtcattgt agccaaccta
     9361 actgtcacgg acaaagatca gccccacacg ccggcctgga atgcggcata cagaatcagt
     9421 ggtggagacc ctacaggaag gtttgccatc ctgacagacc ccaacagcaa tgatgggcta
     9481 gtcacagtgg taaaaccaat tgactttgaa acgaatagga tgtttgtcct tactgttgct
     9541 gcagaaaacc aagtgccatt agctaaaggc attcagcacc cacctcagtc gacagccact
     9601 gtgtctgtga cagttattga tgtcaatgaa aatccttatt ttgccccaaa tcctaaaatc
     9661 attcgccaag aggaaggcct ccacgcaggt accatgctga ccacgctcac tgctcaggac
     9721 cccgatcgat atatgcaaca gaatatcaga tacacaaaat tgtctgatcc tgccaactgg
     9781 ctgaaaatag accccgtgaa tgggcagatc actactattg ccgttttgga cagagaatcg
     9841 ccaaatgtaa aaaacaacat ctataatgct accttccttg cttctgacaa tggaatcccg
     9901 cctatgagtg ggacaggaac actgcaaatc tatttacttg atatcaatga caacgcccct
     9961 caggtgttac ctcaagaggc ggagacctgt gaaactccag aacccaactc aattaacatc
    10021 acagcacttg attatgacat agacccaaac gccgggccgt tcgcgtttga tcttccctta
    10081 tctccagtga ctattaaaag aaactggacc atcaaccggc ttaatggtga ttttgctcag
    10141 ctcaatttaa agataaaatt tttggaagct ggtatctatg aagttcccat cattatcaca
    10201 gattcaggga atccccccaa gtccaacatt tccatcctgc gtgtgaaagt ttgtcagtgt
    10261 gactccaatg gagactgcac ggacgtggac aggatcgtgg gtgcagggct tggcacgggc
    10321 gccatcatcg ctatccttct gtgtatcatc atcctgctga tccttgttct catgtttgtg
    10381 gtatggatga aacggcggga taaagagcgc caagccaagc agcttttaat tgacccagaa
    10441 gatgatgtaa gagataatat attgaaatat gatgaagaag gtggaggaga agaagaccag
    10501 gactatgact tgagccagct ccagcaacca gatactgtgg agcctgatgc catcaagccc
    10561 gtgggaatca gacggctaga cgagaggcct atccatgctg agccacagta cccagtccga
    10621 tccgcagccc cacaccctgg ggatattggg gacttcatta atgagggcct taaagctgct
    10681 gacaacgacc ccacggcgcc accgtatgac tccctcttag tctttgacta cgagggcagc
    10741 ggctccacgg ctggctcctt gagctccctc aactcctcca gtagcggtgg ggaccaggac
    10801 tatgactacc tgaatgactg gggaccccgc ttcaagaaac tggcggacat gtacggcggt
    10861 ggtgacgact cgcggccggc cagcggcagc ggcgccacaa acttctctct gctaaagcaa
    10921 gcaggtgatg ttgaagaaaa ccccgggcct catatggaga ccgacaccct cttgctctgg
    10981 gtcctgctgc tgtgggttcc gggtagtacc ggagacatgg tttctaaggg tgaagaattg
    11041 ttcaccggag ttgtgcccat cctggttgag ctggacggag acgttaacgg acacaagttc
    11101 tcagtgagcg gcgaaggaga gggcgacgct acgtacggta aactgacgtt gaagttcatc
    11161 tgtacaacag gaaagctgcc agtgccctgg cctacactcg tgaccaccct cacctacgga
    11221 gtccagtgct tctcccggta ccccgaccac atgaaacagc acgatttctt caagtccgcg
    11281 atgcctgagg gctatgttca ggaacgcacc atattcttca aggacgacgg taactataaa
    11341 accagggctg aagttaagtt cgagggggac acgttggtaa atagaattga gttgaaagga
    11401 atcgatttca aagaagacgg caatattttg ggccacaagc tggagtacaa ctacaacagc
    11461 cacaacgtgt atattatggc tgataagcag aagaacggca tcaaggtgaa cttcaaaatc
    11521 cgacacaata tcgaggatgg ctctgtgcaa ctggccgacc attaccagca aaacacgcct
    11581 atcggcgacg gtccggtttt gctgccagat aaccactacc tctccacgca atcagccctc
    11641 tccaaggatc ctaacgaaaa aagagaccat atggttctgc ttgaattcgt gacggctgct
    11701 gggatcaccc tgggaatgga tgagctgtat aagaacgccg tgggccagga tacgcaggag
    11761 gtgatcgtcg tgccccacag cttgcccttc aaggtcgtgg ttattagtgc tatcctggcg
    11821 ttggtggtgc tgactattat ctcccttatc atacttatca tgctgtggca aaaaaaaccc
    11881 cgataagcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc gcgactctag
    11941 agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
    12001 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    12061 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    12121 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    12181 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    12241 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    12301 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    12361 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    12421 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    12481 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    12541 gagtcggatc tccctttggg ccgcctcccc gcat
//
