LOCUS Exported 11661 bp ds-DNA circular SYN 13-SEP-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11661) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Sep 20, 2019 from SnapGene Viewer 4.3.11 https://www.snapgene.com FEATURES Location/Qualifiers source 1..11661 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(177..765) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(936..1796) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1797..1901) /gene="bla" /label=AmpR promoter misc_feature 1939..2548 /label=ARS7 primer_bind 3003..3019 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 3041..3640 /label=CPAR2_808670p (CpGAPDHp) primer_bind 3430..3449 /label=GAPDHp_FWD misc_feature 3659..3731 /label=tRNA-Ala C. parapsilosis misc_RNA 3754..3829 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 3834..3901 /label=HDV Ribozyme misc_feature 3902..4001 /label=CPAR2_808670t (GAPDHt) primer_bind complement(3920..3943) /label=GAPDHt_REV misc_feature 4023..4701 /label=CpTEF1p CDS complement(4044..4061) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 4712..8815 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 8828..8848 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature 8858..9733 /label=CpTEF1t CDS complement(9076..9090) /codon_start=1 /product="enterokinase recognition and cleavage site" /label=enterokinase site /translation="DDDDK" CDS 9744..9764 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature 9804..11003 /label=CaACT1p CDS 11004..11528 /codon_start=1 /transl_table=12 /label=CaSAT1 /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSPY RKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSL IEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMY WYWFSGAQDDA" misc_feature 11529..11661 /label=CaURA3 ORIGIN 1 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 61 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 1081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 1801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 1861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 1921 ccacctgacg tcgtgtacat aggcgtatca cgaggccctt tcgtctcgcg cgatacctct 1981 tactgatatt tcttgcgttg tgatcgagct tatggatgag gcaatcaccg ggccaatcta 2041 ctacgagaga ccgcgatata tttaattcat tattttgagg gttattgcaa caatctacgg 2101 aggtgttgaa gggcgccatt ctatgaagtt caggaaaaag aaaatgttgc tggttgatgt 2161 gtatcaaggg atgagcatga acgctcgttt aaaaggatat tgttattgat ccaaggggcc 2221 attgtttccc acaaacgtgc tcaacattac tacctgattg ccgtaaaact gatcccttcc 2281 ggaaagccaa ttgaattatt ttgtgtttga tcataaattg acgcaaattt gaaacaaatt 2341 tttgaattta tgataaccac ttaatcgact gctcttagag actgtaactc aagaatccaa 2401 ccacaaaccc tcatggactc ctttcggtgc ctaaccaaga gaatattccc acagattagc 2461 gaagcgtgct agaatcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2521 cccggagacg gtcacagctt gtctgtaagt atcagacgtc taagaaacca ttattatcat 2581 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 2641 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 2701 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 2761 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 2821 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 2881 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 2941 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 3001 ttgtaaaacg acggccagtg aattggagat cggtacttcg gtactttggt gtaactgggg 3061 tattttgccc ggtgataaaa tagaagggac gtgggaatag ttgtgagtgg aaattgttga 3121 tccgatatgt tgaggaaaag tccgatgtga atcgaatgat agaggtatta ctttaaataa 3181 ggaaaagggt gtcggttggg ttgtgagttt gtgcgtctaa gcattgtcag tcgtccgtca 3241 tcatttgcag accgaacccc attgagctag ggtcgtagaa ccacgtaggt agggtgagat 3301 tagcagtgct gtatatccga tggacgatta tgacccggcg gtatgacttt gttgcagcgc 3361 gagtgtgcac tattgtacaa aaagcaaaat taaaaatgaa tatatgctac catatgattt 3421 ttaactacat acgttctact ctcttccgta ttacaaacaa acaactgaca caaaagaaga 3481 caacaactaa tgagaaaaat gtcacgttga cccgaaatct ttttctcctt tctttgccat 3541 tttctgatta tttaaccaat tgaaatttct caacaaattt ttaaatacaa ttcttcttcc 3601 ttccttcctt ccttctttct ttctttcttt tccaaattca aaacaaagag cttaaaatgg 3661 gcgtgtggcg tagttggtag cgcgttccct tagcatggga aaggtcatga gttcgattct 3721 tatctcgtcc aggaagagct cgcgagctct tccgttttag agctagaaat agcaagttaa 3781 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttggccggc 3841 atggtcccag cctcctcgct ggcgccggct gggcaacatg cttcggcatg gcgaatggga 3901 cctatccgtg aagttataag aaggcaatta agagctagct agctagtctt tggttttctt 3961 ttggttttat atatttttcg tatatgagtt tataaataaa gcgaatgcgt cgagataccg 4021 gtacaccatc attgatgatg atcatgatga tggtggtggt gtcagattgc cctccccgag 4081 aaagtctagg ccaaaacaga aatatggcaa attaatggga agcgtgtcgc cactgtcaat 4141 gagaagtaag cttacggatg catccatgac atcaagatca accattatta tggacgatga 4201 agatgacgat gaaagagaag gtactgatgc ggtaggggct gggttaaatt gtgatgtcac 4261 aaacgatgaa gacttgtctt cgcaagttag gtacgaggac gccgagtcaa tgatgaatca 4321 agaacgtctt ttgaagaaat tggaagagga gtcacctcaa tatagacggg aaacgggatt 4381 gactgcaaat ggtagctcaa caaaacggcg taagactaga gggcagtagc cctattacta 4441 cagaccacgt gacctttatc agatctgtta acttcaattt tttgagaaca atttggcata 4501 tctaggatgt ttaaaatata tagcgaacca attaaagacc atcccgctga ccactacact 4561 tgcaagacga tgctaatagc aaatttgaaa ctagaaaggt ggtgtgcttt gaccaattgg 4621 tagaacttac atcttcttac aacgaattct acggtgctct actagtgcat cctattgttg 4681 catcatagac ttagagagtg accggtccgg tatggacaag aagtactcta tcggtttgga 4741 catcggtact aactctgttg gttgggctgt tatcactgac gaatacaagg ttccatctaa 4801 gaagttcaag gttttgggta acactgacag acactctatc aagaagaact tgatcggtgc 4861 tttgttgttc gactctggtg aaactgctga agctactaga ttgaagagaa ctgctagaag 4921 aagatacact agaagaaaga acagaatctg ttacttgcaa gaaatcttct ctaacgaaat 4981 ggctaaggtt gacgactctt tcttccacag attggaagaa tctttcttgg ttgaagaaga 5041 caagaagcac gaaagacacc caatcttcgg taacatcgtt gacgaagttg cttaccacga 5101 aaagtaccca actatctacc acttgagaaa gaagttggtt gactctactg acaaggctga 5161 cttgagattg atctacttgg ctttggctca catgatcaag ttcagaggtc acttcttgat 5221 cgaaggtgac ttgaacccag acaactctga cgttgacaag ttgttcatcc aattggttca 5281 aacttacaac caattgttcg aagaaaaccc aatcaacgct tctggtgttg acgctaaggc 5341 tatcttgtct gctagattgt ctaagtctag aagattggaa aacttgatcg ctcaattgcc 5401 aggtgaaaag aagaacggtt tgttcggtaa cttgatcgct ttgtctttgg gtttgactcc 5461 aaacttcaag tctaacttcg acttggctga agacgctaag ttgcaattgt ctaaggacac 5521 ttacgacgac gacttggaca acttgttggc tcaaatcggt gaccaatacg ctgacttgtt 5581 cttggctgct aagaacttgt ctgacgctat cttgttgtct gacatcttga gagttaacac 5641 tgaaatcact aaggctccat tgtctgcttc tatgatcaag agatacgacg aacaccacca 5701 agacttgact ttgttgaagg ctttggttag acaacaattg ccagaaaagt acaaggaaat 5761 cttcttcgac caatctaaga acggttacgc tggttacatc gacggtggtg cttctcaaga 5821 agaattctac aagttcatca agccaatctt ggaaaagatg gacggtactg aagaattgtt 5881 ggttaagttg aacagagaag acttgttgag aaagcaaaga actttcgaca acggttctat 5941 cccacaccaa atccacttgg gtgaattgca cgctatcttg agaagacaag aagacttcta 6001 cccattcttg aaggacaaca gagaaaagat cgaaaagatc ttgactttca gaatcccata 6061 ctacgttggt ccattggcta gaggtaactc tagattcgct tggatgacta gaaagtctga 6121 agaaactatc actccatgga acttcgaaga agttgttgac aagggtgctt ctgctcaatc 6181 tttcatcgaa agaatgacta acttcgacaa gaacttgcca aacgaaaagg ttttgccaaa 6241 gcactctttg ttgtacgaat acttcactgt ttacaacgaa ttgactaagg ttaagtacgt 6301 tactgaaggt atgagaaagc cagctttctt gtctggtgaa caaaagaagg ctatcgttga 6361 cttgttgttc aagactaaca gaaaggttac tgttaagcaa ttgaaggaag actacttcaa 6421 gaagatcgaa tgtttcgact ctgttgaaat ctctggtgtt gaagacagat tcaacgcttc 6481 tttgggtact taccacgact tgttgaagat catcaaggac aaggacttct tggacaacga 6541 agaaaacgaa gacatcttgg aagacatcgt tttgactttg actttgttcg aagacagaga 6601 aatgatcgaa gaaagattga agacttacgc tcacttgttc gacgacaagg ttatgaagca 6661 attgaagaga agaagataca ctggttgggg tagattgtct agaaagttga tcaacggtat 6721 cagagacaag caatctggta agactatctt ggacttcttg aagtctgacg gtttcgctaa 6781 cagaaacttc atgcaattga tccacgacga ctctttgact ttcaaggaag acatccaaaa 6841 ggctcaagtt tctggtcaag gtgactcttt gcacgaacac atcgctaact tggctggttc 6901 tccagctatc aagaagggta tcttgcaaac tgttaaggtt gttgacgaat tggttaaggt 6961 tatgggtaga cacaagccag aaaacatcgt tatcgaaatg gctagagaaa accaaactac 7021 tcaaaagggt caaaagaact ctagagaaag aatgaagaga atcgaagaag gtatcaagga 7081 attgggttct caaatcttga aggaacaccc agttgaaaac actcaattgc aaaacgaaaa 7141 gttgtacttg tactacttgc aaaacggtag agacatgtac gttgaccaag aattggacat 7201 caacagattg tctgactacg acgttgacca catcgttcca caatctttct tgaaggacga 7261 ctctatcgac aacaaggttt tgactagatc tgacaagaac agaggtaagt ctgacaacgt 7321 tccatctgaa gaagttgtta agaagatgaa gaactactgg agacaattgt tgaacgctaa 7381 gttgatcact caaagaaagt tcgacaactt gactaaggct gaaagaggtg gtttgtctga 7441 attggacaag gctggtttca tcaagagaca attggttgaa actagacaaa tcactaagca 7501 cgttgctcaa atcttggact ctagaatgaa cactaagtac gacgaaaacg acaagttgat 7561 cagagaagtt aaggttatca ctttgaagtc taagttggtt tctgacttca gaaaggactt 7621 ccaattctac aaggttagag aaatcaacaa ctaccaccac gctcacgacg cttacttgaa 7681 cgctgttgtt ggtactgctt tgatcaagaa gtacccaaag ttggaatctg aattcgttta 7741 cggtgactac aaggtttacg acgttagaaa gatgatcgct aagtctgaac aagaaatcgg 7801 taaggctact gctaagtact tcttctactc taacatcatg aacttcttca agactgaaat 7861 cactttggct aacggtgaaa tcagaaagag accattgatc gaaactaacg gtgaaactgg 7921 tgaaatcgtt tgggacaagg gtagagactt cgctactgtt agaaaggttt tgtctatgcc 7981 acaagttaac atcgttaaga agactgaagt tcaaactggt ggtttctcta aggaatctat 8041 cttgccaaag agaaactctg acaagttgat cgctagaaag aaggactggg acccaaagaa 8101 gtacggtggt ttcgactctc caactgttgc ttactctgtt ttggttgttg ctaaggttga 8161 aaagggtaag tctaagaagt tgaagtctgt taaggaattg ttgggtatca ctatcatgga 8221 aagatcttct ttcgaaaaga acccaatcga cttcttggaa gctaagggtt acaaggaagt 8281 taagaaggac ttgatcatca agttgccaaa gtactctttg ttcgaattgg aaaacggtag 8341 aaagagaatg ttggcttctg ctggtgaatt gcaaaagggt aacgaattgg ctttgccatc 8401 taagtacgtt aacttcttgt acttggcttc tcactacgaa aagttgaagg gttctccaga 8461 agacaacgaa caaaagcaat tgttcgttga acaacacaag cactacttgg acgaaatcat 8521 cgaacaaatc tctgaattct ctaagagagt tatcttggct gacgctaact tggacaaggt 8581 tttgtctgct tacaacaagc acagagacaa gccaatcaga gaacaagctg aaaacatcat 8641 ccacttgttc actttgacta acttgggtgc tccagctgct ttcaagtact tcgacactac 8701 tatcgacaga aagagataca cttctactaa ggaagttttg gacgctactt tgatccacca 8761 atctatcact ggtttgtacg aaactagaat cgacttgtct caattgggtg gtgactctag 8821 agctgaccca aagaagaaga gaaaggttta aatcggatcc attatgtagg tgaatggtga 8881 tttgtttttg aacaacttgt tgtttaaaat cctgttcaac acagaatcaa actgtatatt 8941 ctgattaagt tcaggagcat aatgatgata gtcattcaat gcctttttga cggctttggt 9001 tgtcgtcaga ttgccatttt tataaatgtt ttcttggagt tctgcagcag acatccactc 9061 tttagtggtt gcagttttat catcatcatc agaatcagat tcaatttcct gcaacaacat 9121 aaacccatca ccagcacaac aacatcccaa taaatcatgg gtgtttgact tggtatccac 9181 gtcaatttca aacaagtttt tcaagtcgct ataatcaaac acatctgatt tcgatgaagc 9241 atcgttgtca agaaactttg aactcaattt gttcttcatc aactgccgtt ggaatatctt 9301 ctcatcgata caaccagtag tgaaaagcct gtatatataa cacggcttca tttggccatc 9361 tcgatgaatc ctcgacattg attgcaaatc tgtagatgga ttccaatcat tatcgtatag 9421 gattagcctt gacgcaccaa ccaaattgat accagtccca cccgatttcg aggatagtaa 9481 aaacaccaga atcagttcgt ccttgttgaa cttgttgacg agcttgcctc tcatgttgtt 9541 tggtgttgat ccatccaagc gcaagtattg tagattgagc tttttgatca cagtctccaa 9601 caagtccaaa gtagtggtgt agtttgaaat gagcactaat ttctcttttt gtgctgtaat 9661 ctccaacacc aatggtatga gtgcatttag tttacctgag ctagtagata atttcaagct 9721 tccatcggtt ataggatcct gacccaaaga agaagagaaa ggtttaaggg atcccgggcc 9781 cgtcgactgc agaggcctgc atgcgtcaaa actagagaat aataaagaaa acgatctttt 9841 caaaaagaaa aaacctttta gttttccttt gttgttgttg tgggtgtgtg ctatttatat 9901 tatatagttt actcataata ccataaaata ttcggtttga ttaggttatt ttaataagct 9961 aatttgtttc taatcgtgta atttatgctg tgtatattag gtagtgtgtg cactgcccaa 10021 aaatgtttgt tgtttatagt cggttaaaga gaaaaaagaa aaaaagatcc atacacacac 10081 gttaattagt tgttcaacgt aatacactca tattttgttc ttatttgctt tcggtcgctg 10141 ttctcaccaa gatttattgc caacgaaaca attttttttt atatattttc agatttttct 10201 ttttttcctt tcctttcctt ttctaatttt cactcctggt tttctttctt tcttagaaac 10261 attatctcga tattaatatt aaaaaaatat aatcattcaa aatggacggt ggtatgtttt 10321 agtttagctt caattctaat tgattgatta atcagttgat tggtttcaat atgacaaatg 10381 ggtagggtgg gaaaacttca ttttcaattc agatcaaact tttttgttgt cgacataata 10441 tttctcgttt gggatgttac tgtcacatta ataatacaca cacatcagct tataattttg 10501 aaagtaattt atcagatatg ttgtgacgat cagtggaaat ggctaacttc aatgtatctg 10561 ttcttcccct ttttcaaagt tcacgatttt tgattgattg attgatctgt cggcagtggt 10621 ttcaaaacca ttcggtgagt aatcctacca atcaatgtta cgacaaaagg ctcaatattc 10681 aaaattgcaa tgttttatgt tttcctacgt gtacttgtgc aaggcaattg attcaacatt 10741 gcttttggtg tttgacgagt ttctagtttg gacttgtgtt gttatctggg ctatacagat 10801 ttcccggctc actatgaatt ttttttttcg acgctcagtg cacacaacta taaacaacac 10861 aaacacaaac acagcaagaa aaaaaaaaaa cgaacattga attgaaacca agccaactga 10921 aaaattcctt atttaaatga ctgtcatact aacccatttt tatagaagaa gttgctgctt 10981 tagttatcga taacggttct catatgaaaa tttcggtgat ccctgagcag gtggcggaaa 11041 cattggatgc tgagaaccat ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc 11101 aaggctttga actatctacc agaagtgtga gcccctaccg gaaggattac atctcggatg 11161 atgactctga tgaagactct gcttgctatg gcgcattcat cgaccaagag cttgtcggga 11221 agattgaact caactcaaca tggaacgatc tagcctctat cgaacacatt gttgtgtcgc 11281 acacgcaccg aggcaaagga gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac 11341 taagcagaca gctccttggc atacgattag agacacaaac gaacaatgta cctgcctgca 11401 atttgtacgc aaaatgtggc tttactctcg gcggcattga cctcttcacg tataaaacta 11461 gacctcaagt ctcgaacgaa acagcgatgt actggtactg gttctcggga gcacaggatg 11521 acgcctaaca tatgtgaagt gtgaaggggg agattttcac tttattagat ttgtatatat 11581 gtataataaa taaataaata agttaaataa ataattagat aagggtggta attattacta 11641 tttacaatca aaggtggtcc t //