LOCUS       pmApple-EGFP-HRas\2        6037 bp    DNA     circular     11-JAN-2019
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12400848|
COMMENT     VNTDBDATE|-12637019|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pmApple-EGFP-HRas 2|
COMMENT     VNTAUTHORNAME|Piston lab|
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FEATURES             Location/Qualifiers
     misc_signal     2008..2010
                     /vntifkey="87"
                     /label=A206
     misc_feature    2119..2685
                     /vntifkey="21"
                     /label=HRas
     misc_signal     1390..2106
                     /vntifkey="87"
                     /label=EGFP
     rep_origin      5312..5955
                     /vntifkey="33"
                     /label=ColE1\ORI
                     /note="ColE1 Origin Of Replication"
     polyA_signal    4728..5183
                     /vntifkey="25"
                     /label=HSV\TK\polyA
                     /note="Herpes simplex virus thymidine kinase polyadenylation signal"
     misc_feature    3573..3811
                     /vntifkey="21"
                     /label=SV40\early\promoter
                     /note="Simian Virus 40 early promoter"
     misc_feature    3749..3884
                     /vntifkey="21"
                     /label=SV40\ORI
                     /note="Simian Virus 40 Origin of Replication"
     misc_feature    3470..3498
                     /vntifkey="21"
                     /label=Bact.\promoter
                     /note="Bacterial promoter for expression Kan R gene"
     rep_origin      2953..3408
                     /vntifkey="33"
                     /label=f1\ORI
                     /note="f1 Origin of Replication (packages the noncoding strand of EGFP"
     polyA_signal    2856..2890
                     /vntifkey="25"
                     /label=SV40\polyA
                     /note="Simian Virus 40 polyadenylation signal"
     misc_marker     3933..4727
                     /vntifkey="22"
                     /label=Kan\/\Neo\resistance
                     /note="Kanamycin / Neomycin resistance"
     promoter        1..589
                     /vntifkey="29"
                     /label=CMV\promoter
                     /note="CMV promoter"
     misc_feature    628..1335
                     /vntifkey="21"
                     /label=mApple
                     /note="mApple"
     unsure          1336..1389
                     /vntifkey="99"
                     /label=linker
     unsure          2107..2118
                     /vntifkey="99"
                     /label=linker
BASE COUNT     1436 a      1665 c      1648 g      1288 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttatg gtgagcaagg gcgaggagaa taacatggcc 
      661 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 
      721 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg 
      781 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 
      841 tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt caagctgtcc 
      901 ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac 
      961 gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa gctgcgcggc 
     1021 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 
     1081 gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa gaggctgaag 
     1141 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc 
     1201 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag 
     1261 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 
     1321 gacgagctgt acaagtccgg actcagatct cgagcggtac cgcgggcccg ggatccaccg 
     1381 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 
     1441 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 
     1501 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 
     1561 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 
     1621 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 
     1681 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 
     1741 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 
     1801 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 
     1861 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 
     1921 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 
     1981 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 
     2041 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 
     2101 tacaagtact cagatctcat gaccgaatac aagcttgttg ttgttggcgc cggcggtgtg 
     2161 ggcaagagtg cgctgaccat ccagctgatc cagaaccatt ttgtggacga atacgacccc 
     2221 actatagagg attcctaccg gaagcaggtg gtcattgatg gggagacgtg cctgttggac 
     2281 atcctggata ccgccggcca ggaggagtac agcgccatgc gggaccagta catgcgcacc 
     2341 ggggagggct tcctgtgtgt gtttgccatc aacaacacca agtcttttga ggacatccac 
     2401 cagtacaggg agcagatcaa acgggtgaag gactcggatg acgtgcccat ggtgctggtg 
     2461 gggaacaagt gtgacctggc tgcacgcact gtggaatctc ggcaggctca ggacctcgcc 
     2521 cgaagctacg gcatccccta catcgagacc tcggccaaga cccggcaggg agtggaggat 
     2581 gccttctaca cgttggtgcg tgagatccgg cagcacaagc tgcggaagct gaaccctcct 
     2641 gatgagagtg gccccggctg catgagctgc aagtgtgtgc tctcctgagg gcccgggatc 
     2701 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc 
     2761 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 
     2821 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     2881 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     2941 atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 
     3001 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 
     3061 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 
     3121 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 
     3181 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 
     3241 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 
     3301 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 
     3361 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt 
     3421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     3481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc 
     3541 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 
     3601 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 
     3661 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     3721 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     3781 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 
     3841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga 
     3901 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 
     3961 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
     4021 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
     4081 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 
     4141 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
     4201 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
     4261 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
     4321 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
     4381 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
     4441 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 
     4501 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
     4561 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
     4621 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
     4681 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 
     4741 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 
     4801 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 
     4861 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac 
     4921 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa 
     4981 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 
     5041 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 
     5101 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 
     5161 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta 
     5221 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     5281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     5341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     5401 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     5461 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 
     5521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     5581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     5641 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     5701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     5761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     5821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     5881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     5941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     6001 ccctgattct gtggataacc gtattaccgc catgcat 
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