LOCUS       pEGFP-GNB1        5784 bp    DNA     circular     19-SEP-2018
ACCESSION   pVec1
VERSION     pVec1.1
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12472766|
COMMENT     VNTDBDATE|-12472766|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEGFP-GNB1|
COMMENT     VNTAUTHORNAME|Piston lab|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     misc_signal     613..1329
                     /vntifkey="87"
                     /label=EGFP
     misc_feature    1384..2406
                     /vntifkey="21"
                     /label=GNB1
     promoter        1..589
                     /vntifkey="29"
                     /label=CMV\promoter
     unsure          1330..1383
                     /vntifkey="99"
                     /label=linker
     rep_origin      2700..3155
                     /vntifkey="33"
                     /label=f1\ORI
     misc_feature    3320..3558
                     /vntifkey="21"
                     /label=SV40\early\promoter
     misc_feature    3496..3631
                     /vntifkey="21"
                     /label=SV40\ORI
     misc_marker     3680..4474
                     /vntifkey="22"
                     /label=kan/neo\resistance
     rep_origin      5059..5702
                     /vntifkey="33"
                     /label=ColE1\ORI
BASE COUNT     1377 a      1591 c      1524 g      1292 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcactagt gattgccgcc 
     1381 accatgagtg agcttgacca gttacggcag gaggccgagc aacttaagaa ccagattcga 
     1441 gacgccagga aagcatgtgc agatgcaact ctctctcaga tcacaaacaa catcgaccca 
     1501 gtgggaagaa tccaaatgcg cacgaggagg acactgcggg ggcacctggc caagatctac 
     1561 gccatgcact ggggcacaga ctccaggctt ctcgtcagtg cctcgcagga tggtaaactt 
     1621 atcatctggg acagctacac caccaacaag gtccacgcca tccctctgcg ctcctcctgg 
     1681 gtcatgacct gtgcatatgc cccttctggg aactatgtgg cctgcggtgg cctggataac 
     1741 atttgctcca tttacaatct gaaaactcgt gaggggaacg tgcgcgtgag tcgtgagctg 
     1801 gcaggacaca caggttacct gtcctgctgc cgattcctgg atgacaatca gatcgtcacc 
     1861 agctctggag acaccacgtg tgccctgtgg gacatcgaga ccggccagca gacgaccacg 
     1921 tttaccggac acactggaga tgtcatgagc ctttctcttg ctcctgacac cagactgttc 
     1981 gtctctggtg cttgtgatgc ttcagccaaa ctctgggatg tgcgagaagg catgtgccgg 
     2041 cagaccttca ctggccacga gtctgacatc aatgccattt gcttctttcc aaatggcaat 
     2101 gcatttgcca ctggctcaga cgacgccacc tgcaggctgt ttgaccttcg tgctgaccag 
     2161 gagctcatga cttactccca tgacaacatc atctgcggga tcacctctgt ctccttctcc 
     2221 aagagcgggc gcctcctcct tgctgggtac gacgacttca actgcaacgt ctgggatgca 
     2281 ctcaaagccg accgggcagg tgtcttggct gggcatgaca accgcgtcag ctgcctgggc 
     2341 gtgactgacg atggcatggc tgtggcgaca gggtcctggg atagcttcct caagatctgg 
     2401 aactaagaat cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga 
     2461 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 
     2521 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 
     2581 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     2641 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 
     2701 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 
     2761 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 
     2821 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 
     2881 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 
     2941 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 
     3001 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 
     3061 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 
     3121 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 
     3181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     3241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 
     3301 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 
     3361 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 
     3421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 
     3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     3541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 
     3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 
     3661 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 
     3721 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 
     3781 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 
     3841 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 
     3901 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 
     3961 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 
     4021 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 
     4081 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 
     4141 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 
     4201 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 
     4261 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 
     4321 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 
     4381 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 
     4441 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 
     4501 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 
     4561 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 
     4621 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 
     4681 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 
     4741 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 
     4801 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 
     4861 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 
     4921 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 
     4981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 
     5041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 
     5101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 
     5161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 
     5221 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 
     5281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 
     5341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 
     5401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 
     5461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 
     5521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 
     5581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 
     5641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 
     5701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 
     5761 gataaccgta ttaccgccat gcat 
//
