LOCUS       pmApple-GNG2        5250 bp    DNA     circular     22-JUL-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|686939956|
COMMENT     VNTDBDATE|-11336388|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pmApple-GNG2|
COMMENT     VNTAUTHORNAME|Piston lab|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #10=(CFSignal (CObList) "SV40 polyA" 25 0 0 2069 2103 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "SV40 ORI" 21 0 0 2962 3097 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "GNG2" 54 0 0 1327 1539 0 (CStringList) 
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COMMENT                1 2 "?" 0) 
COMMENT          #111=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #114=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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COMMENT          #121=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #122=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #123=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #124=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
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COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 65.2174 65.2174 39 39 0.1 
COMMENT         -5250) 1 1 0 1 1 
COMMENT        (mapper: 20.3445 -11.7419 96.1149 96.1149 0.01 10 -1 5250 5250 1 0 0) 
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COMMENT                            0 -10 8.31297 1.39416 Nil) 
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COMMENT                            (LOGPEN 0 0 153) 1 
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COMMENT                                   "@N" 1 -4.89885 -12.1963 0 -10 6.12808 
COMMENT                                   1.10285 #147#) 
COMMENT                             #149=(CWideArrow 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                    0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                   1 -10.6967 -8.71165 0 -10 3.88077 1.10285 
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COMMENT                                    (LOGPEN 0 0 6723840) 1 
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COMMENT                                   "SV40 early promoter" "@N" 1 -10.8642 -10.454 
COMMENT                                   0 -10 8.29216 1.10285 #151#) 
COMMENT                             #153=(CWideArrow 
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COMMENT                                   12.8892 5.22699 0 -10 3.18369 1.10285 #153#)) 
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COMMENT                                   "Kan / Neo resistance" "@N" 1 -15.3998 
COMMENT                                   -5.22699 0 -10 8.43782 1.10285 #156#)) 
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COMMENT                                   #159#) 
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COMMENT                                   (LOGFONT 111 42 0 0 700 0 0 0 0 3 2 1 34 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #176=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1186 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList #177=(XLATFRAGMENT 612 308 0 1 613 926)) 
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FEATURES             Location/Qualifiers
     promoter        1..589
                     /vntifkey="29"
                     /label=CMV\promoter
                     /note="CMV promoter"
     misc_marker     3146..3940
                     /vntifkey="22"
                     /label=Kan\/\Neo\resistance
                     /note="Kanamycin / Neomycin resistance"
     polyA_signal    2069..2103
                     /vntifkey="25"
                     /label=SV40\polyA
                     /note="Simian Virus 40 polyadenylation signal"
     rep_origin      2166..2621
                     /vntifkey="33"
                     /label=f1\ORI
                     /note="f1 Origin of Replication (packages the noncoding strand of EGFP"
     misc_feature    2683..2711
                     /vntifkey="21"
                     /label=Bact.\promoter
                     /note="Bacterial promoter for expression Kan R gene"
     misc_feature    2962..3097
                     /vntifkey="21"
                     /label=SV40\ORI
                     /note="Simian Virus 40 Origin of Replication"
     misc_feature    2786..3024
                     /vntifkey="21"
                     /label=SV40\early\promoter
                     /note="Simian Virus 40 early promoter"
     polyA_signal    4176..4194
                     /vntifkey="25"
                     /label=HSV\TK\polyA
                     /note="Herpes simplex virus thymidine kinase polyadenylation signal"
     rep_origin      4525..5168
                     /vntifkey="33"
                     /label=ColE1\ORI
                     /note="ColE1 Origin Of Replication"
     mRNA            1327..1539
                     /vntifkey="54"
                     /label=GNG2
     misc_feature    613..1320
                     /vntifkey="21"
                     /label=mApple
BASE COUNT     1301 a      1380 c      1385 g      1184 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagaataaca tggccatcat caaggagttc 
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
      721 gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt gaccaagggt 
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggtc 
      841 tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc cgagggcttc 
      901 aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa ccaggactcc 
      961 tccctgcagg acggcgtgtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccgagga gcggatgtac 
     1081 cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa ggacggcggc 
     1141 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 
     1201 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg 
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 
     1321 tccggaatgg ccagcaacaa caccgccagc atagcacaag ccaggaagct ggtagaacag 
     1381 ctgaagatgg aagccaacat cgacaggata aaggtgtcca aggcagctgc tgacttgatg 
     1441 gcctactgtg aggcacatgc caaggaagac cctctgctga ccccagtccc agcctcagaa 
     1501 aacccctttc gggagaagaa gttcttctgc gccatccttt aagtctctga gaggaggctg 
     1561 aagagtgttg gggctcctgg gacatacatg tagagttcct agcaaagtgg gcgcctttct 
     1621 cgtccacagc atttaaagag aggaaggaga accatcctgg acactccggg ctgtgcatgt 
     1681 ttaaagaaat gtccccttat gagaatgaaa gctgattccg tgtcccaact ttagagatct 
     1741 gaccctgcag accggcctgg aggagggaaa tgtataaaaa atgagaatgg taatcacttc 
     1801 ttttctgctg tccctctaag acatttctgc ttcatattta taaacaaaat aaacattaaa 
     1861 gccggtgtcc tgagctgaga aaaaaaaaaa aaaaaaaggg gcggcccggg atccaccgga 
     1921 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 
     1981 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 
     2041 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     2101 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 
     2161 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
     2221 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
     2281 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
     2341 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
     2401 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
     2461 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
     2521 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
     2581 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 
     2641 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 
     2701 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 
     2761 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
     2821 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 
     2881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 
     2941 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     3001 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     3061 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 
     3121 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 
     3181 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 
     3241 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 
     3301 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 
     3361 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 
     3421 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 
     3481 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 
     3541 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 
     3601 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 
     3661 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 
     3721 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 
     3781 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 
     3841 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 
     3901 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 
     3961 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 
     4021 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 
     4081 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 
     4141 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 
     4201 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 
     4261 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 
     4321 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 
     4381 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 
     4441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 
     4501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     4561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     4621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     4681 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     4741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     4801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     4861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     4921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     4981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     5041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     5101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 
     5161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 
     5221 tctgtggata accgtattac cgccatgcat 
//
