LOCUS       pCD86(1-19)-EGFP-CD86(20-323)-mApple        6509 bp    DNA     circular     27-MAR-2019
ACCESSION   pVec1
VERSION     pVec1.1
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12554951|
COMMENT     VNTDBDATE|-12745041|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD86(1-19)-EGFP-CD86(20-323)-mApple|
COMMENT     VNTAUTHORNAME|Piston lab|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     misc_feature    679..735
                     /vntifkey="21"
                     /label=CD86\1-19
     misc_feature    1489..2400
                     /vntifkey="21"
                     /label=CD86\20-323
     unsure          2401..2463
                     /vntifkey="99"
                     /label=linker
     promoter        1..589
                     /vntifkey="29"
                     /label=CMV
     rep_origin      3328..3378
                     /vntifkey="33"
                     /label=SV40\ori
     misc_feature    4405..5199
                     /vntifkey="21"
                     /label=kan/neo\r
     rep_origin      5784..6427
                     /vntifkey="33"
                     /label=pUC
     unsure          736..756
                     /vntifkey="99"
                     /label=linker
     unsure          1474..1488
                     /vntifkey="99"
                     /label=linker
     misc_feature    757..1473
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    2464..3171
                     /vntifkey="21"
                     /label=mApple
BASE COUNT     1622 a      1741 c      1688 g      1458 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcgccaccat gggactgagt aacattctct ttgtgatggc cttcctgctc 
      721 tctggtgctg ctcctctgaa gattccggtc gccaccatgg tgagcaaggg cgaggagctg 
      781 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 
      841 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 
      901 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 
      961 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 
     1021 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 
     1081 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 
     1141 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 
     1201 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 
     1261 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 
     1321 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 
     1381 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 
     1441 gggatcactc tcggcatgga cgagctgtac aagtccggaa tggcccctct gaagattcaa 
     1501 gcttatttca atgagactgc agacctgcca tgccaatttg caaactctca aaaccaaagc 
     1561 ctgagtgagc tagtagtatt ttggcaggac caggaaaact tggttctgaa tgaggtatac 
     1621 ttaggcaaag agaaatttga cagtgttcat tccaagtata tgggccgcac aagttttgat 
     1681 tcggacagtt ggaccctgag acttcacaat cttcagatca aggacaaggg cttgtatcaa 
     1741 tgtatcatcc atcacaaaaa gcccacagga atgattcgca tccaccagat gaattctgaa 
     1801 ctgtcagtgc ttgctaactt cagtcaacct gaaatagtac caatttctaa tataacagaa 
     1861 aatgtgtaca taaatttgac ctgctcatct atacacggtt acccagaacc taagaagatg 
     1921 agtgttttgc taagaaccaa gaattcaact atcgagtatg atggtattat gcagaaatct 
     1981 caagataatg tcacagaact gtacgacgtt tccatcagct tgtctgtttc attccctgat 
     2041 gttacgagca atatgaccat cttctgtatt ctggaaactg acaagacgcg gcttttatct 
     2101 tcacctttct ctatagagct tgaggaccct cagcctcccc cagaccacat tccttggatt 
     2161 acagctgtac ttccaacagt tattatatgt gtgatggttt tctgtctaat tctatggaaa 
     2221 tggaagaaga agaagcggcc tcgcaactct tataaatgtg gaaccaacac aatggagagg 
     2281 gaagagagtg aacagaccaa gaaaagagaa aaaatccata tacctgaaag atctgatgaa 
     2341 acccagcgtg tttttaaaag ttcgaagaca tcttcatgcg acaaaagtga tacatgtttt 
     2401 ggaggcggac cggtgcccca gtgggaggga ttcgctgcgc tgctggcaac cccggtcgct 
     2461 actatggtga gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc 
     2521 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag 
     2581 ggccgcccct acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg 
     2641 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag 
     2701 cacccagccg acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag 
     2761 cgcgtgatga acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag 
     2821 gacggcgtgt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc 
     2881 gtaatgcaga agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac 
     2941 ggcgccctga agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc 
     3001 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc 
     3061 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac 
     3121 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaagcggc 
     3181 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 
     3241 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 
     3301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     3361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag 
     3421 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 
     3481 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 
     3541 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 
     3601 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 
     3661 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 
     3721 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 
     3781 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 
     3841 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga 
     3901 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     3961 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg 
     4021 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
     4081 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     4141 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     4201 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     4261 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     4321 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa 
     4381 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 
     4441 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 
     4501 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 
     4561 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 
     4621 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 
     4681 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 
     4741 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 
     4801 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 
     4861 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 
     4921 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 
     4981 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 
     5041 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 
     5101 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 
     5161 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 
     5221 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 
     5281 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 
     5341 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg 
     5401 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg 
     5461 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata 
     5521 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc 
     5581 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca 
     5641 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     5701 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     5761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     5821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     5881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     5941 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     6001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     6061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     6121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     6181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     6241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     6301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     6361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     6421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     6481 ctgtggataa ccgtattacc gccatgcat 
//
