LOCUS       Exported                3401 bp ds-DNA     circular SYN 24-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 24, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     385..401
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        408..426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    508..556
                     /note="synthetic polyadenylation signal"
     misc_feature    570..661
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_binding    674..680
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    688..694
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    698..704
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    707..713
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    716..722
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    724..730
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     primer_bind     746..768
                     /label=SEQ MINIP FWD
     promoter        784..815
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(825..847)
                     /label=SEQ MiniP REV
     promoter        complement(950..968)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(986..1002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1010..1026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1034..1064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1079..1100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1388..1976)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2147..3007)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3008..3112)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(3190..3401,1..244))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gctggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
       61 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
      121 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
      181 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
      241 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      301 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      361 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
      421 tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt
      481 cgattgggcg atgagtggtc tcgggagaat aaaatatctt tattttcatt acatctgtgt
      541 gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa
      601 caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc
      661 tcgctgcaga ctatgagtca aacagcgtga ctcaagttga gtcagttgac tcacttgagt
      721 cactgactca ggcatgctcc ctcgaggata tcaaatctgg cctcggcggc caagcttaga
      781 cactagaggg tatataatgg aagctcgact tccagcttgg caatccggta ctgttggtaa
      841 agccaccaat gcgagaccac tcatcgccca atcactagtg aattcgcggc cgcctgcagg
      901 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
      961 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1021 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1081 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1141 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1201 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     1261 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1321 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1381 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1441 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1501 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1561 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1621 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1681 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1741 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1801 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1861 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1921 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1981 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2041 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2101 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2161 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2221 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2281 taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2341 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2401 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2461 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2521 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2581 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2641 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2701 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2761 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2821 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2881 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2941 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3001 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3061 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3121 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     3181 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3241 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3301 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3361 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg c
//
