LOCUS       pBS_PB_GS56925          3680 bp    DNA        circular     17-JAN-2024
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    262..278
                     /note="M13R"
                     /locus_tag="M13rev"
     misc_feature    299..317
                     /note="T3"
                     /locus_tag="T3"
     misc_feature    complement(328..734)
                     /note="3' piggyBac sequence"
                     /locus_tag="3' piggyBac sequence"
     misc_feature    377..380
                     /note="TTAA"
                     /locus_tag="TTAA(1)"
     misc_feature    381..393
                     /note="13 bp terminal inverted repeat"
                     /locus_tag="13-bp TR(1)"
     misc_feature    394..424
                     /note="31 bp spacer"
                     /locus_tag="31 bp spacer"
     misc_feature    425..443
                     /note="19 bp internal inverted repeat"
                     /locus_tag="19-bp IR"
     misc_feature    703..703
                     /note="A to G mutation"
                     /locus_tag="A to G mutation"
     misc_feature    736..749
                     /note="Insert"
                     /locus_tag="Insert"
     misc_feature    complement(751..1157)
                     /note="5' piggyBac sequence"
                     /locus_tag="5' piggyBac sequence"
     misc_feature    1045..1063
                     /note="19 bp internal inverted repeat"
                     /locus_tag="19-bp TR"
     misc_feature    1064..1066
                     /note="3 bp spacer"
                     /locus_tag="3 bp spacer"
     misc_feature    1067..1079
                     /note="13 bp terminal inverted repeat"
                     /locus_tag="13-bp TR"
     misc_feature    1080..1083
                     /note="TTAA"
                     /locus_tag="TTAA"
     misc_feature    1167..1185
                     /note="T7"
                     /locus_tag="T7"
     misc_feature    1192..1208
                     /note="M13F"
                     /locus_tag="M13for"
     CDS             complement(1482..1788)
                     /locus_tag="f1 origin"
     CDS             1937..2794
                     /locus_tag="Amp+"
     misc_feature    complement(2945..3612)
                     /locus_tag="pUC origin"
ORIGIN
        1 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
       61 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      121 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      181 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      241 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
      301 ttaaccctca ctaaagggaa cctcccccgg tattcacgac agcaggctga ataataaaaa
      361 aattagaaac tattatttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata
      421 atcatgcgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa
      481 tttgaataga tattaagttt tattatattt acacttacat actaataata aattcaacaa
      541 acaatttatt tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat
      601 aaagtaacaa aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta
      661 aaaacagtca tgttgtatta taaaataagt aattagctta acctatacat aatagaaaca
      721 aattatactt attaaccggt cagtaagctt ttttattata tattagtcac gatatctata
      781 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
      841 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
      901 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
      961 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     1021 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     1081 taaaaaagat ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg
     1141 acaaaaacca cattgtgttg ggtcgcccta tagtgagtcg tattacaatt cactggccgt
     1201 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1261 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1321 acagttgcgc agcctgaatg gcgaatggca aattgtaagc gttaatattt tgttaaaatt
     1381 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     1441 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     1501 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     1561 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     1621 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     1681 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     1741 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     1801 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1861 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1921 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1981 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2041 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     2101 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     2161 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     2221 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     2281 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     2341 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     2401 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     2461 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     2521 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     2581 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2641 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2701 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2761 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3661 cgcctttgag tgagctgata    
//