LOCUS       p15A_PB                 3471 bp    DNA        circular     17-JAN-2024
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    196..215
                     /note="Rev primer"
                     /locus_tag="Rev primer(1)"
     misc_feature    216..265
                     /note="Recombineering homology arm"
                     /locus_tag="Recombineering homology"
     misc_feature    265..281
                     /note="M13R"
                     /locus_tag="M13rev"
     misc_feature    302..320
                     /note="T3"
                     /locus_tag="T3"
     misc_feature    380..383
                     /note="TTAA"
                     /locus_tag="TTAA(1)"
     misc_feature    384..396
                     /note="13 bp terminal inverted repeat"
                     /locus_tag="13-bp TR(1)"
     misc_feature    397..427
                     /note="31 bp spacer"
                     /locus_tag="31 bp spacer"
     misc_feature    428..446
                     /note="19 bp internal inverted repeat"
                     /locus_tag="19-bp IR"
     misc_feature    739..752
                     /note="Insert"
                     /locus_tag="Insert"
     misc_feature    1048..1066
                     /note="19 bp internal inverted repeat"
                     /locus_tag="19-bp TR"
     misc_feature    1067..1069
                     /note="3 bp spacer"
                     /locus_tag="3 bp spacer"
     misc_feature    1070..1082
                     /note="13 bp terminal inverted repeat"
                     /locus_tag="13-bp TR"
     misc_feature    1083..1086
                     /note="TTAA"
                     /locus_tag="TTAA"
     misc_feature    1170..1188
                     /note="T7"
                     /locus_tag="T7"
     misc_feature    1195..1211
                     /note="M13F"
                     /locus_tag="M13for"
     misc_feature    1251..1300
                     /note="Recombineering homology arm"
                     /locus_tag="Recombineering homology(1)"
     misc_feature    1301..1320
                     /note="For primer"
                     /locus_tag="For primer(1)"
     rep_origin      1340..2250
                     /note="p15A origin of replication"
                     /locus_tag="p15A origin of replication"
     gene            2579..3394
                     /gene="kan"
                     /locus_tag="kan"
     CDS             2579..3394
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin resistance protein"
                     /db_xref="GI:186703036"
                     /protein_id=ACC91752.1
                     /translation=MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGEGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLV
                     DASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGR
                     VGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
                     /locus_tag="kan(1)"
     misc_feature    706..706
                     /note="A to G mutation"
                     /locus_tag="A to G mutation"
     misc_feature    complement(754..1160)
                     /locus_tag="5' piggyBac sequence"
     misc_feature    complement(331..737)
                     /locus_tag="3' piggyBac sequence"
ORIGIN
        1 ctaactacta agcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
       61 gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
      121 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga
      181 cgcccgccat aaactgccag gcatcaaact aagcacttcc ggctcgtatg ttgtgtggaa
      241 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      301 caattaaccc tcactaaagg gaacctcccc cggtattcac gacagcaggc tgaataataa
      361 aaaaattaga aactattatt taaccctaga aagataatca tattgtgacg tacgttaaag
      421 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      481 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      541 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca aaatttcttc
      601 tataaagtaa caaaactttt aaacattctc tcttttacaa aaataaactt attttgtact
      661 ttaaaaacag tcatgttgta ttataaaata agtaattagc ttaacctata cataatagaa
      721 acaaattata cttattaacc ggtcagtaag cttttttatt atatattagt cacgatatct
      781 ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat
      841 aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac
      901 tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac
      961 gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta
     1021 gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag
     1081 ggttaaaaaa gatttgcgct ttactcgacc taaactttaa acacgtcata gaatcttcgt
     1141 ttgacaaaaa ccacattgtg ttgggtcgcc ctatagtgag tcgtattaca attcactggc
     1201 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1261 agcacatccc cctttcgcca gctggcgtaa tagcgaagag ttatttgcca tccagctgca
     1321 gctgtcaaac atgagaatta caacttatat cgtatggggc tgacttcagg tgctacattt
     1381 gaagagataa attgcactga aatctagaaa tattttatct gattaataag atgatcttct
     1441 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
     1501 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
     1561 ggagcgcagt caccagaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
     1621 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
     1741 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     1801 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     1861 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1921 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     1981 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
     2041 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
     2101 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     2161 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
     2221 acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa gaaggtgttg
     2281 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt
     2341 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg
     2401 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat
     2461 ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2521 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2581 gagccatatt caacgggaaa catcttgctc gaggccgcga ttaaattcca acatggatgc
     2641 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2701 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggcg aaggtagcgt
     2761 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2821 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2881 ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2941 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     3001 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     3061 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     3121 aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     3181 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3241 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3301 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3361 gcagtttcat ttgatgctcg atgagttttt ctaatcagac atgttctttc ctgcgttatc
     3421 ccctgattct gtggataacc gtattaccgc tagcatggat ctcggggacg t
//