LOCUS       Exported               10880 bp ds-DNA     circular SYN 28-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAWH_IRP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Mireia
  TITLE     Direct Submission
  JOURNAL   Exported 23 Sep 2019 from SnapGene 2.6.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1491..1493
                     /codon_start=1
                     /product="start codon for expression in yeast"
                     /note="ATG"
                     /translation="M"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /note="GAL4 activation domain"
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     promoter        1904..1922
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1935..1937
                     /codon_start=1
                     /product="start codon for in vitro 
                     transcription/translation"
                     /note="ATG"
                     /translation="M"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    1968..2017
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             2018
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /note="GAL4 activation domain"
                     /translation=""
     protein_bind    2025..2049
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2052..4714
                     /codon_start=1
                     /product="218..2887
                     /gene=""ACO1""
                     /gene_synonym=""FRP""
                     /EC_number=""4.2.1.3""
                     /note=""IRP1; IRE-BP 1; citrate hydro-lyase; iron
                     regulatory protein 1; iron-responsive element-binding
                     protein 1; ferritin repressor protein""
                     /codon_start=1
                     /product=""cytoplasmic aconitate hydratase""
                     /protein_id=""NP_001075784.1""
                     /db_xref=""GI:126722605""
                     /db_xref=""GeneID:100009154"""
                     /note="IRP1"
                     /note="Vector made by Wickens for positive control in yeast
                     three hybrid. I didn't have the sequence from wikens, so 
                     this sequence has been assembled by partial sequencing and 
                     blasting for IRP gene in NCBI. "
                     /translation="MSNPFAYLAEPLDPAQPGKKFFNLNKLDYSRYGRLPFSIRVLLEA
                     AVRNCDKFLVKKEDIENILNWNVTQHMNIEVPFKPARVILQDFTGVPSVVDFAAMRDAV
                     KKLGGDPEKINPICPVDLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSKA
                     FRNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGVLGWGVG
                     GIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFF
                     GLGVAQLSIADRATIANMCPEYGATATFFPVDEVSIKYLVQTGRDESKVKQIRKYLQAV
                     GMFRDYSDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGF
                     KGFQVAPDHHNDHKTFIYNDSEFTLSHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVD
                     AGLNVKPYVKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPV
                     VEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLG
                     TNAKGQQVFLRDIWPTREEIQAVERQYVIPGMFTEVYQKIETVNASWNALAAPSDKLYL
                     WNPKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAAR
                     YLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNRFLNKQAPQTIHLPSGETLDVF
                     DAAERYQQEGHPLIVLAGKEYGSGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMG
                     VIPLEYLPGENADSLGLTGRERYTIIIPENLTPRMHVQVKLDTGKTFQAVIRFDTDVEL
                     TYLHNGGILNYMIRKM"
     protein_bind    complement(4719..4743)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4754..4771
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    4922..4981
                     /note="H1_overlap_site"
     misc_feature    4978..5038
                     /note="Self_overlap_site"
     terminator      5191..5378
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
     CDS             complement(5495..6589)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /note="LEU2"
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6590..6995)
                     /gene="S. cerevisiae LEU2"
                     /note="LEU2 promoter"
     primer_bind     complement(7037..7053)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7061..7077
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7085..7115)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7130..7151
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    complement(7206..7239)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(7317..7350)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    7379..7439
                     /note="Self_overlap_site"
     misc_feature    7436..7495
                     /note="H2_overlap_site"
     rep_origin      complement(7707..8295)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8466..9326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9327..9431)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      9713..10877
                     /note="2u ori"
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
     1981 aggccagtga attccacccg ggtgggcatc gatacgggat ctccacaagt ttgtacaaaa
     2041 aagcaggctt aatgagcaac ccatttgcat accttgctga gccgttagat cctgcacaac
     2101 caggaaagaa gttcttcaat ttgaataaat tagactattc aagatatgga cgcttaccgt
     2161 tttcaatcag agttctcctg gaggcagccg ttcggaactg tgataagttt ttggtgaaga
     2221 aagaagacat tgaaaatatc ctgaactgga atgtcacgca gcatatgaac atagaagtgc
     2281 cattcaaacc cgcccgtgtc atcctgcagg acttcacggg tgtaccatct gtggttgact
     2341 ttgctgcaat gcgtgatgct gtgaaaaaat tagggggtga tccagagaaa ataaacccta
     2401 tctgtcctgt tgatcttgta atcgatcact ccatccaggt tgatttcaac agaagggctg
     2461 acagcttaca gaagaatcaa gacctagaat ttgaaagaaa tagagaacga tttgaatttt
     2521 taaagtgggg ttccaaggct tttcgaaaca tgcggattat ccctcccggc tcaggaatca
     2581 tccaccaagt gaatctggaa tacttggcca gggtagtgtt tgatcaggat ggatactact
     2641 accccgacag cctcgtgggc accgattcgc acacgaccat gattgacggt ttgggcgttc
     2701 ttggctgggg tgtgggtggc attgaagcag aagctgtcat gctgggtcag cctatcagta
     2761 tggtgcttcc ccaggtgatt ggctacaggc tgatgggaaa gccccaccct ctggtaacat
     2821 ccactgacat cgtgctcacc attaccaagc acctccgcca ggttggggtc gtgggcaaat
     2881 ttgttgagtt cttcgggcta ggagtagccc agttgtccat tgctgacaga gctacaatcg
     2941 ccaacatgtg tccagaatac ggagcaactg ctaccttctt cccagttgat gaagtcagta
     3001 tcaagtactt ggtgcagaca ggtcgggatg aaagcaaagt gaagcagatt agaaagtatc
     3061 ttcaggctgt agggatgttc cgagattaca gtgacccctc tcaagaccca gacttcaccc
     3121 aggttgtgga attagatttg aagaccgtag tgccttgctg cagtggaccc aagaggcctc
     3181 aggacaaagt tgctgtgtct gatatgaaaa aggactttga gagctgcctt ggagccaagc
     3241 aaggatttaa aggattccag gttgctccag accatcataa tgaccataag acgtttatct
     3301 ataatgatag tgaattcacc ctttctcatg gatctgtggt aattgctgcc attactagct
     3361 gcacaaacac cagtaatcca tctgtgatgt taggagcagg actgttagcg aagaaagccg
     3421 tggatgctgg cttgaacgtg aagccctacg tgaagaccag cctgtcccct ggaagtggtg
     3481 tggtcaccta ctacctgcga gagagtggag tcatgcctta cttgtctcag ctggggtttg
     3541 atgtggtggg ctatggctgc atgacctgca tcggcaacag tggaccctta cctgagcctg
     3601 tggtagaagc catcacacag ggagaccttg tagctgtcgg agtactctct gggaacagaa
     3661 attttgaagg tcgagttcat cccaacaccc gggccaacta tttggcctct cctcccttag
     3721 taatagcata tgcaattgct gggaccatca ggatcgactt tgagaaagag ccattgggga
     3781 cgaacgcaaa ggggcagcag gtgtttctga gagatatctg gccaactcga gaggagatcc
     3841 aggcagtgga gcggcagtac gtcatccctg ggatgttcac ggaggtctac cagaaaatag
     3901 agactgtgaa tgcaagctgg aatgccttag cagccccatc agataagctg tatctctgga
     3961 atcccaaatc cacatacatt aagtcaccac cattctttga aaacctgact ttggacctgc
     4021 agccccctaa atctatagtg gatgcctatg tgctgttaaa tttgggagat tcagtaacga
     4081 cggaccacat ctctccagct ggaaatattg caagaaatag ccctgctgct cgctacttaa
     4141 ccaacagagg gctaactcca cgagaattca actcctatgg ctcccgccgg gggaatgatg
     4201 ccatcatggc acgggggacg ttcgccaaca ttcgcttgtt aaacagattt ttgaacaagc
     4261 aggcaccaca gactatacat ctgccttctg gagaaaccct tgatgtgttt gatgcagctg
     4321 agcggtacca gcaggaaggc catcccctga tcgtccttgc tggcaaggag tatggctcag
     4381 gcagctcccg agactgggca gccaagggcc ctttcctgct gggaatcaaa gctgtcctgg
     4441 ctgagagtta cgagcgcatt caccgcagta acctggttgg aatgggggtg atcccccttg
     4501 aatacctccc tggtgagaac gcagactccc tgggactcac aggacgggaa cgatacacca
     4561 tcatcattcc agaaaacctc acaccacgga tgcatgtcca ggtcaagctg gacaccggga
     4621 agaccttcca ggccgtcata cggtttgaca ctgacgtgga gctcacttat ttgcacaatg
     4681 gcggcatact caactacatg atccgcaaga tggcatagac ccagctttct tgtacaaagt
     4741 ggtaagtaac taagatccat cgagctcgag ctgcagatga atcgtagata ctgaaaaacc
     4801 ccgcaagttc acttcaactg tgcatcgtgc accatctcaa tttctttcat ttatacatcg
     4861 ttttgccttc ttttatgtaa ctatactcct ctaagtttca atcttggcca tgtaacctct
     4921 gataacaggg taattggcaa acagctatta tgggtattat gggtgtttaa actagggata
     4981 acagggtaat acattcaagc gcattagtaa tgagtgaata taactataac ggtcctaacg
     5041 agggcttatt cagaagcttt ggacttcttc gccagaggtt tggtcaagtc tccaatcaag
     5101 gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg tcaaatcgtt
     5161 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
     5221 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     5281 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     5341 tagcatgagg tcgctcttat tgaccacacc tctaccggcc ggtcgaaatt cccctaccct
     5401 atgaacatat tccattttgt aatttcgtgt cgtttctatt atgaatttca tttataaagt
     5461 ttatgtacaa atatcataaa aaaagagaat ctttttaagc aaggattttc ttaacttctt
     5521 cggcgacagc atcaccgact tcggtggtac tgttggaacc acctaaatca ccagttctga
     5581 tacctgcatc caaaaccttt ttaactgcat cttcaatggc cttaccttct tcaggcaagt
     5641 tcaatgacaa tttcaacatc attgcagcag acaagatagt ggcgataggg ttgaccttat
     5701 tctttggcaa atctggagca gaaccgtggc atggttcgta caaaccaaat gcggtgttct
     5761 tgtctggcaa agaggccaag gacgcagatg gcaacaaacc caaggaacct gggataacgg
     5821 aggcttcatc ggagatgata tcaccaaaca tgttgctggt gattataata ccatttaggt
     5881 gggttgggtt cttaactagg atcatggcgg cagaatcaat caattgatgt tgaaccttca
     5941 atgtaggaaa ttcgttcttg atggtttcct ccacagtttt tctccataat cttgaagagg
     6001 ccaaaacatt agctttatcc aaggaccaaa taggcaatgg tggctcatgt tgtagggcca
     6061 tgaaagcggc cattcttgtg attctttgca cttctggaac ggtgtattgt tcactatccc
     6121 aagcgacacc atcaccatcg tcttcctttc tcttaccaaa gtaaatacct cccactaatt
     6181 ctctgacaac aacgaagtca gtacctttag caaattgtgg cttgattgga gataagtcta
     6241 aaagagagtc ggatgcaaag ttacatggtc ttaagttggc gtacaattga agttctttac
     6301 ggatttttag taaaccttgt tcaggtctaa cactacctgt accccattta ggaccaccca
     6361 cagcacctaa caaaacggca tcagccttct tggaggcttc cagcgcctca tctggaagtg
     6421 ggacacctgt agcttcgata gcagcaccac caattaaatg attttcgaaa tcgaacttga
     6481 cattggaacg aacatcagaa atagctttaa gaaccttaat ggcttcggct gtgatttctt
     6541 gaccaacgtg gtcacctggc aaaacgacga tcttcttagg ggcagacatt agaatggtat
     6601 atccttgaaa tatatatata tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa
     6661 gacgattgct aaccacctat tggaaaaaac aataggtcct taaataatat tgtcaacttc
     6721 aagtattgtg atgcaagcat ttagtcatga acgcttctct attctatatg aaaagccggt
     6781 tccggcgctc tcacctttcc tttttctccc aatttttcag ttgaaaaagg tatatgcgtc
     6841 aggcgacctc tgaaattaac aaaaaatttc cagtcatcga atttgattct gtgcgatagc
     6901 gcccctgtgt gttctcgtta tgttgaggaa aaaaataatg gttgctaaga gattcgaact
     6961 cttgcatctt acgatacctg agtattccca cagttggggg atctcgactc tagctagagg
     7021 atcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7081 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7141 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7201 agctgataac ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac
     7261 gtccagccaa gctagcttgg ctgcaggtcg agcggccgcg atccggaacc cttaatataa
     7321 cttcgtataa tgtatgctat acgaagttat cagctgcatt aatgaatcgg ccaacgcgat
     7381 aacagggtaa tacattcaag cgcattagta atgagtgaat ataactataa cggtcctaag
     7441 gtagcgaaag aatcaccagt tacatatatg gtgatagtaa ctataacggt cctaacgggg
     7501 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7561 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7621 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8161 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8221 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8281 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8341 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8401 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8461 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8521 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8581 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8641 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8701 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8761 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8821 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8881 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8941 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9001 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9061 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9121 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9181 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9241 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9301 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9361 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9421 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9481 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9541 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9601 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9661 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca taacgcattt
     9721 aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag
     9781 atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag
     9841 cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagctagaa
     9901 agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa
     9961 aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac cgcttccaca
    10021 aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct atataaccta
    10081 cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat tttttatgtt
    10141 tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca tagagtgaat
    10201 cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac aaaatagaag
    10261 aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt tctgttcaca
    10321 aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat cttgaaaaaa
    10381 tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg ctttttttat
    10441 ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct acagtgcaaa
    10501 aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc
    10561 tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag aagtatagat
    10621 tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag
    10681 attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa aaatgaagca
    10741 cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag
    10801 ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc tacaaaatga
    10861 agcacagatg cttcgttgct
//
