LOCUS       Exported                1176 bp ds-DNA     linear   SYN 23-SEP-2019
DEFINITION  RH339_T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 23, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             66..83
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /note="color: #cc99b2
                     Cleavage site after base 77"
                     /translation="LVPRGS"
     CDS             573..590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     terminator      657..704
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff"
     rep_origin      741..1121
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
       61 gcggcctggt gccgcgcggc agccatatga tgtccggaat tgccctctct cgcttagctc
      121 aggaacgcaa agcgtggcgc aaagatcacc cgtttgggtt tgttgccgtg cctaccaaaa
      181 atcctgacgg tacgatgaat cttatgaact gggaatgcgc gatccctggc aagaaaggca
      241 caccgtggga aggcggcttg ttcaagctgc gtatgttgtt caaagatgat tacccgtcaa
      301 gtccgcctaa atgtaaattc gagccgccgt tatttcatcc aaacgtgtat cccagcggta
      361 cggtttgcct gagcatcctt gaagaagaca aagattggcg tcccgcaatt accatcaagc
      421 agattctgct cggcattcaa gagctgttaa atgaaccaaa cattcaggac ccagcgcagg
      481 cagaagccta tactatctac tgtcagaacc gtgtagagta tgagaagcgc gtccgtgcac
      541 aagcgaaaaa gtttgccccg tcgtaactcg agcaccacca ccaccaccac tgagatccgg
      601 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      661 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      721 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      781 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      841 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      901 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      961 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1021 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     1081 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     1141 gctgatttaa caaaatttaa cssgaatttt aacaaa
//
